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UniProtKB - P07897 (PGCA_RAT)

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Protein

Aggrecan core protein

Gene

Acan

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region. May play a regulatory role in the matrix assembly of the cartilage.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi1975Calcium 11
Metal bindingi1979Calcium 11
Metal bindingi1979Calcium 31
Metal bindingi1999Calcium 21
Metal bindingi2001Calcium 21
Metal bindingi2002Calcium 11
Metal bindingi2008Calcium 1; via carbonyl oxygen1
Metal bindingi2008Calcium 21
Metal bindingi2009Calcium 11
Metal bindingi2009Calcium 31
Metal bindingi2022Calcium 21
Metal bindingi2023Calcium 21
Metal bindingi2023Calcium 2; via carbonyl oxygen1

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

GO - Biological processi

  • cell adhesion Source: InterPro
  • cellular response to growth factor stimulus Source: RGD
  • central nervous system development Source: RGD
  • chondroblast differentiation Source: RGD
  • multicellular organism aging Source: RGD
  • negative regulation of cell migration Source: RGD
  • ossification Source: RGD
  • response to acidic pH Source: RGD
  • response to drug Source: RGD
  • response to glucose Source: RGD
  • response to gravity Source: RGD
  • response to mechanical stimulus Source: RGD
  • response to organic cyclic compound Source: RGD
  • response to radiation Source: RGD
  • spinal cord development Source: RGD
View the complete GO annotation on QuickGO ...

Keywordsi

LigandLectin, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Aggrecan core protein
Alternative name(s):
Cartilage-specific proteoglycan core protein
Short name:
CSPCP
Gene namesi
Name:Acan
Synonyms:Agc, Agc1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi68358 Acan

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 191 PublicationAdd BLAST19
ChainiPRO_000001750820 – 2124Aggrecan core proteinAdd BLAST2105

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi51 ↔ 133By similarity
Glycosylationi126N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi175 ↔ 246By similarity
Disulfide bondi199 ↔ 220By similarity
Glycosylationi239N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi273 ↔ 348By similarity
Disulfide bondi297 ↔ 318By similarity
Glycosylationi333N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi371O-linked (Xyl...) (keratan sulfate) threonineBy similarity1
Glycosylationi376O-linked (Xyl...) (keratan sulfate) threonineBy similarity1
Glycosylationi387N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi509 ↔ 580By similarity
Disulfide bondi533 ↔ 554By similarity
Disulfide bondi607 ↔ 682By similarity
Glycosylationi611N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi631 ↔ 652By similarity
Glycosylationi667N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1842N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi1914 ↔ 1925By similarity
Disulfide bondi1942 ↔ 20341 Publication
Disulfide bondi2010 ↔ 20261 Publication
Disulfide bondi2041 ↔ 2084By similarity
Disulfide bondi2070 ↔ 2097By similarity

Post-translational modificationi

Contains mostly chondroitin sulfate, but also keratan sulfate chains, N-linked and O-linked oligosaccharides.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Proteoglycan

Proteomic databases

PaxDbiP07897
PRIDEiP07897

PTM databases

iPTMnetiP07897

Interactioni

Subunit structurei

Interacts with FBLN1 and COMP.By similarity

Protein-protein interaction databases

IntActiP07897, 1 interactor
STRINGi10116.ENSRNOP00000042691

Structurei

Secondary structure

12124
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi1919 – 1921Combined sources3
Beta strandi1924 – 1933Combined sources10
Helixi1935 – 1944Combined sources10
Helixi1955 – 1965Combined sources11
Beta strandi1969 – 1974Combined sources6
Beta strandi1976 – 1978Combined sources3
Turni2005 – 2007Combined sources3
Beta strandi2008 – 2013Combined sources6
Turni2015 – 2019Combined sources5
Beta strandi2021 – 2025Combined sources5
Beta strandi2030 – 2036Combined sources7

3D structure databases

ProteinModelPortaliP07897
SMRiP07897
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07897

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini34 – 147Ig-like V-typeAdd BLAST114
Domaini153 – 248Link 1PROSITE-ProRule annotationAdd BLAST96
Domaini254 – 350Link 2PROSITE-ProRule annotationAdd BLAST97
Domaini487 – 582Link 3PROSITE-ProRule annotationAdd BLAST96
Domaini588 – 684Link 4PROSITE-ProRule annotationAdd BLAST97
Domaini1910 – 2036C-type lectinPROSITE-ProRule annotationAdd BLAST127
Domaini2039 – 2099SushiPROSITE-ProRule annotationAdd BLAST61

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni48 – 140G1-AAdd BLAST93
Regioni152 – 247G1-BAdd BLAST96
Regioni253 – 349G1-B'Add BLAST97
Regioni486 – 580G2-BAdd BLAST95
Regioni587 – 682G2-B'Add BLAST96
Regioni685 – 798KSAdd BLAST114
Regioni801 – 1226CS-1Add BLAST426
Regioni1227 – 1909CS-2Add BLAST683
Regioni1910 – 2124G3Add BLAST215

Domaini

Two globular domains, G1 and G2, comprise the N-terminus of the proteoglycan, while another globular region, G3, makes up the C-terminus. G1 contains Link domains and thus consists of three disulfide-bonded loop structures designated as the A, B, B' motifs. G2 is similar to G1. The keratan sulfate (KS) and the chondroitin sulfate (CS) attachment domains lie between G2 and G3.

Sequence similaritiesi

Belongs to the aggrecan/versican proteoglycan family.Curated

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Sushi

Phylogenomic databases

eggNOGiENOG410IJP2 Eukaryota
ENOG410XRES LUCA
HOGENOMiHOG000168421
HOVERGENiHBG007982
InParanoidiP07897
KOiK06792

Family and domain databases

CDDicd00033 CCP, 1 hit
cd03588 CLECT_CSPGs, 1 hit
Gene3Di2.60.40.10, 1 hit
3.10.100.10, 5 hits
InterProiView protein in InterPro
IPR001304 C-type_lectin-like
IPR016186 C-type_lectin-like/link_sf
IPR018378 C-type_lectin_CS
IPR033987 CSPG_CTLD
IPR016187 CTDL_fold
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR003006 Ig/MHC_CS
IPR003599 Ig_sub
IPR013106 Ig_V-set
IPR000538 Link_dom
IPR035976 Sushi/SCR/CCP_sf
IPR000436 Sushi_SCR_CCP_dom
PfamiView protein in Pfam
PF00059 Lectin_C, 1 hit
PF00084 Sushi, 1 hit
PF07686 V-set, 1 hit
PF00193 Xlink, 4 hits
PRINTSiPR01265 LINKMODULE
SMARTiView protein in SMART
SM00032 CCP, 1 hit
SM00034 CLECT, 1 hit
SM00409 IG, 1 hit
SM00406 IGv, 1 hit
SM00445 LINK, 4 hits
SUPFAMiSSF48726 SSF48726, 1 hit
SSF56436 SSF56436, 5 hits
SSF57535 SSF57535, 1 hit
PROSITEiView protein in PROSITE
PS00615 C_TYPE_LECTIN_1, 1 hit
PS50041 C_TYPE_LECTIN_2, 1 hit
PS50835 IG_LIKE, 1 hit
PS00290 IG_MHC, 1 hit
PS01241 LINK_1, 4 hits
PS50963 LINK_2, 4 hits
PS50923 SUSHI, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P07897-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTTLLLVFVT LRVIAAVISE EVPDHDNSLS VSIPQPSPLK ALLGTSLTIP 
        60         70         80         90        100
CYFIDPMHPV TTAPSTAPLT PRIKWSRVSK EKEVVLLVAT EGQVRVNSIY 
       110        120        130        140        150
QDKVSLPNYP AIPSDATLEI QNLRSNDSGI YRCEVMHGIE DSEATLEVIV 
       160        170        180        190        200
KGIVFHYRAI STRYTLDFDR AQRACLQNSA IIATPEQLQA AYEDGFHQCD 
       210        220        230        240        250
AGWLADQTVR YPIHTPREGC YGDKDEFPGV RTYGIRDTNE TYDVYCFAEE 
       260        270        280        290        300
MEGEVFYATS PEKFTFQEAA NECRTVGARL ATTGQLYLAW QGGMDMCSAG 
       310        320        330        340        350
WLADRSVRYP ISKARPNCGG NLLGVRTVYL HANQTGYPDP SSRYDAICYT 
       360        370        380        390        400
GEDFVDIPEN FFGVGGEEDI TIQTVTWPDL ELPLPRNITE GEARGNVILT 
       410        420        430        440        450
AKPIFDMSPT VSEPGEALTL APEVGTTVFP EAGERTEKTT RPWGFPEEAT 
       460        470        480        490        500
RGPDSATAFA SEDLVVRVTI SPGAVEVPGQ PRLPGGVVFH YRPGSTRYSL 
       510        520        530        540        550
TFEEAQQACI RTGAAIASPE QLQAAYEAGY EQCDAGWLQD QTVRYPIVSP 
       560        570        580        590        600
RTPCVGDKDS SPGVRTYGVR PSSETYDVYC YVDKLEGEVF FATQMEQFTF 
       610        620        630        640        650
QEAQAFCAAQ NATLASTGQL YAAWSQGLDK CYAGWLADGT LRYPIVNPRP 
       660        670        680        690        700
ACGGDKPGVR TVYLYPNQTG LPDPLSKHHA FCFRGVSVVP SPGGTPTSPS 
       710        720        730        740        750
DIEDWIVTRV EPGVDAVPLE PETTEVPYFT TEPEKQTEWE PAYTPVGTSP 
       760        770        780        790        800
LPGIPPTWLP TVPAAEEHTE SPSASQEPSA SQVPSTSEEP YTPSLAVPSG 
       810        820        830        840        850
TELPSSGDTS GAPDLSGDFT GSTDTSGRLD SSGEPSGGSE SGLPSGDLDS 
       860        870        880        890        900
SGLGPTVSSG LPVESGSASG DGEIPWSSTP TVDRLPSGGE SLEGSASASG 
       910        920        930        940        950
TGDLSGLPSG GEITETSASG TEEISGLPSG GDDLETSTSG IDGASVLPTG 
       960        970        980        990       1000
RGGLETSASG VEDLSGLPSG EEGSETSTSG IEDISVLPTG ESPETSASGV 
      1010       1020       1030       1040       1050
GDLSGLPSGG ESLETSASGV EDVTQLPTER GGLETSASGI EDITVLPTGR 
      1060       1070       1080       1090       1100
ENLETSASGV EDVSGLPSGK EGLETSASGI EDISVFPTEA EGLETSASGG 
      1110       1120       1130       1140       1150
YVSGIPSGED GTETSTSGVE GVSGLPSGGE GLETSASGVE DLGLPTRDSL 
      1160       1170       1180       1190       1200
ETSASGVDVT GYPSGREDTE TSVPGVGDDL SGLPSGQEGL ETSASGAEDL 
      1210       1220       1230       1240       1250
GGLPSGKEDL VGSASGALDF GKLPSGTLGS GQTPEASGLP SGFSGEYSGV 
      1260       1270       1280       1290       1300
DIGSGPSSGL PDFSGLPSGF PTVSLVDSTL VEVITATTAS ELEGRGTISV 
      1310       1320       1330       1340       1350
SGSGEESGPP LSELDSSADI SGLPSGTELS GQTSGSLDVS GETSGFFDVS 
      1360       1370       1380       1390       1400
GQPFGSSGTG EGTSGIPEVS GQAVRSPDTT EISELSGLSS GQPDVSGEGS 
      1410       1420       1430       1440       1450
GILFGSGQSS GITSVSGETS GISDLSGQPS GFPVLSGTTP GTPDLASGAM 
      1460       1470       1480       1490       1500
SGSGDSSGIT FVDTSLIEVT PTTFREEEGL GSVELSGLPS GETDLSGTSG 
      1510       1520       1530       1540       1550
MVDVSGQSSG AIDSSGLISP TPEFSGLPSG VAEVSGEVSG VETGSSLSSG 
      1560       1570       1580       1590       1600
AFDGSGLVSG FPTVSLVDRT LVESITLAPT AQEAGEGPSS ILEFSGAHSG 
      1610       1620       1630       1640       1650
TPDISGDLSG SLDQSTWQPG WTEASTEPPS SPYFSGDFSS TTDASGESIT 
      1660       1670       1680       1690       1700
APTGSGETSG LPEVTLITSE LVEGVTEPTV SQELGHGPSM TYTPRLFEAS 
      1710       1720       1730       1740       1750
GEASASGDLG GPVTIFPGSG VEASVPEGSS DPSAYPEAGV GVSAAPEASS 
      1760       1770       1780       1790       1800
QLSEFPDLHG ITSASRETDL EMTTPGTEVS SNPWTFQEGT REGSAAPEVS 
      1810       1820       1830       1840       1850
GESSTTSDID AGTSGVPFAT PMTSGDRTEI SGEWSDHTSE VNVTVSTTVP 
      1860       1870       1880       1890       1900
ESRWAQSTQH PTETLQEIGS PNPSYSGEET QTAETAKSLT DTPTLASPEG 
      1910       1920       1930       1940       1950
SGETESTAAD QEQCEEGWTK FQGHCYRHFP DRETWVDAER RCREQQSHLS 
      1960       1970       1980       1990       2000
SIVTPEEQEF VNKNAQDYQW IGLNDRTIEG DFRWSDGHSL QFEKWRPNQP 
      2010       2020       2030       2040       2050
DNFFATGEDC VVMIWHERGE WNDVPCNYQL PFTCKKGTVA CGEPPAVEHA 
      2060       2070       2080       2090       2100
RTLGQKKDRY EISSLVRYQC TEGFVQRHVP TIRCQPSADW EEPRITCTDP 
      2110       2120 
NTYKHRLQKR TMRPTRRSRP SMAH
Length:2,124
Mass (Da):221,118
Last modified:July 1, 1989 - v2
Checksum:iE30BBE61593A34B1
GO
Isoform 2 (identifier: P07897-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1909-1909: A → ADIDECLSSPCLNGATCVDALDTFTCLCLPSYRGDLCEI

Show »
Length:2,162
Mass (Da):225,171
Checksum:iAE948F63BAC16612
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti38P → W AA sequence (PubMed:3693371).Curated1
Sequence conflicti61T → E AA sequence (PubMed:3693371).Curated1
Sequence conflicti149I → L AA sequence (PubMed:3693371).Curated1
Sequence conflicti239N → S AA sequence (PubMed:3693371).Curated1
Sequence conflicti241T → A AA sequence (PubMed:3693371).Curated1
Sequence conflicti275 – 276TV → RL in AAA21000 (PubMed:3693370).Curated2
Sequence conflicti374T → H AA sequence (PubMed:3693371).Curated1
Sequence conflicti377W → E AA sequence (PubMed:3693371).Curated1
Sequence conflicti380L → V AA sequence (PubMed:3693371).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0391961909A → ADIDECLSSPCLNGATCVDA LDTFTCLCLPSYRGDLCEI in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03485 mRNA Translation: AAA21000.1
M13518 mRNA Translation: AAA41836.1
PIRiA92623 A28452
RefSeqiNP_071526.1, NM_022190.1
UniGeneiRn.54503

Genome annotation databases

GeneIDi58968
KEGGirno:58968
UCSCiRGD:68358 rat [P07897-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPGCA_RAT
AccessioniPrimary (citable) accession number: P07897
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: July 1, 1989
Last modified: June 20, 2018
This is version 159 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

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