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Entry version 143 (11 Dec 2019)
Sequence version 3 (11 Jan 2001)
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Protein

Alginate biosynthesis protein AlgA

Gene

algA

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Produces a precursor for alginate polymerization. The alginate layer provides a protective barrier against host immune defenses and antibiotics.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the ‘Function’ section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Co2+1 PublicationNote: Co2+ (for PMI).1 Publication

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

  1. KM=3.03 mM for D-mannose 6-phosphate1 Publication
  2. KM=20.5 µM for D-mannose 1-phosphate1 Publication
  3. KM=29.5 µM for GTP1 Publication
  4. KM=14.2 µM for GDP-D-mannose1 Publication
  1. Vmax=830 nmol/min/mg enzyme for the PMI forward reaction1 Publication
  2. Vmax=5680 nmol/min/mg enzyme for the GMP forward reaction1 Publication
  3. Vmax=5170 nmol/min/mg enzyme for the GMP reverse reaction1 Publication

pH dependencei

Optimum pH is 7.0 for PMI activity, and 7.6 for GMP activity.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: GDP-alpha-D-mannose biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes GDP-alpha-D-mannose from alpha-D-mannose 1-phosphate (GTP route).
Proteins known to be involved in this subpathway in this organism are:
  1. Alginate biosynthesis protein AlgA (algA)
This subpathway is part of the pathway GDP-alpha-D-mannose biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes GDP-alpha-D-mannose from alpha-D-mannose 1-phosphate (GTP route), the pathway GDP-alpha-D-mannose biosynthesis and in Nucleotide-sugar biosynthesis.

Pathwayi: GDP-alpha-D-mannose biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes alpha-D-mannose 1-phosphate from D-fructose 6-phosphate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Alginate biosynthesis protein AlgA (algA)
  2. Phosphomannomutase/phosphoglucomutase (algC)
This subpathway is part of the pathway GDP-alpha-D-mannose biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes alpha-D-mannose 1-phosphate from D-fructose 6-phosphate, the pathway GDP-alpha-D-mannose biosynthesis and in Nucleotide-sugar biosynthesis.

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

  • alginic acid biosynthetic process Source: PseudoCAP
  • GDP-mannose biosynthetic process Source: GO_Central

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionIsomerase, Multifunctional enzyme, Nucleotidyltransferase, Transferase
Biological processAlginate biosynthesis
LigandCobalt, GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
MetaCyc:MONOMER-13384
PAER208964:G1FZ6-3619-MONOMER

BRENDA Comprehensive Enzyme Information System

More...
BRENDAi
2.7.7.13 5087

SABIO-RK: Biochemical Reaction Kinetics Database

More...
SABIO-RKi
P07874

UniPathway: a resource for the exploration and annotation of metabolic pathways

More...
UniPathwayi
UPA00126;UER00423
UPA00126;UER00930

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Alginate biosynthesis protein AlgA
Including the following 2 domains:
Mannose-6-phosphate isomerase (EC:5.3.1.8)
Alternative name(s):
Phosphohexomutase
Phosphomannose isomerase
Short name:
PMI
Mannose-1-phosphate guanylyltransferase (EC:2.7.7.13)
Alternative name(s):
GDP-mannose pyrophosphorylase
Short name:
GMP
Short name:
GMPP
GTP--mannose-1-phosphate guanylyltransferase
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:algA
Synonyms:pmi
Ordered Locus Names:PA3551
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri208964 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002438 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome

Organism-specific databases

Pseudomonas genome database

More...
PseudoCAPi
PA3551

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001942481 – 481Alginate biosynthesis protein AlgAAdd BLAST481

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P07874

PRoteomics IDEntifications database

More...
PRIDEi
P07874

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Monomer.

1 Publication

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

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SMRi
P07874

Database of comparative protein structure models

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ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
ENOG4105CJT Bacteria
COG0662 LUCA
COG0836 LUCA

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
HOG000153549

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P07874

KEGG Orthology (KO)

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KOi
K16011

Identification of Orthologs from Complete Genome Data

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OMAi
PATFACC

Database for complete collections of gene phylogenies

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PhylomeDBi
P07874

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
2.60.120.10, 1 hit
3.90.550.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR006375 Man1P_GuaTrfase/Man6P_Isoase
IPR001538 Man6P_isomerase-2_C
IPR005835 NTP_transferase_dom
IPR029044 Nucleotide-diphossugar_trans
IPR014710 RmlC-like_jellyroll
IPR011051 RmlC_Cupin_sf

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF01050 MannoseP_isomer, 1 hit
PF00483 NTP_transferase, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF51182 SSF51182, 1 hit
SSF53448 SSF53448, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR01479 GMP_PMI, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P07874-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MIPVILSGGS GSRLWPLSRK QYPKQFLALT GDDTLFQQTI KRLAFDGMQA
60 70 80 90 100
PLLVCNKEHR FIVQEQLEAQ NLASQAILLE PFGRNTAPAV AIAAMKLVAE
110 120 130 140 150
GRDELLLILP ADHVIEDQRA FQQALALATN AAEKGEMVLF GIPASRPETG
160 170 180 190 200
YGYIRASADA QLPEGVSRVQ SFVEKPDEAR AREFVAAGGY YWNSGMFLFR
210 220 230 240 250
ASRYLEELKK HDADIYDTCL LALERSQHDG DLVNIDAATF ECCPDNSIDY
260 270 280 290 300
AVMEKTSRAC VVPLSAGWND VGSWSSIWDV HAKDANGNVT KGDVLVHDSH
310 320 330 340 350
NCLVHGNGKL VSVIGLEDIV VVETKDAMMI AHKDRVQDVK HVVKDLDAQG
360 370 380 390 400
RSETQNHCEV YRPWGSYDSV DMGGRFQVKH ITVKPGARLS LQMHHHRAEH
410 420 430 440 450
WIVVSGTAQV TCDDKTFLLT ENQSTYIPIA SVHRLANPGK IPLEIIEVQS
460 470 480
GSYLGEDDIE RLEDVYGRTA EPALQVVAGS R
Length:481
Mass (Da):53,128
Last modified:January 11, 2001 - v3
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i2D497AF9059AEF97
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti26 – 27FL → LV in AAA25972 (PubMed:3089876).Curated2
Sequence conflicti115I → L in AAA25972 (PubMed:3089876).Curated1
Sequence conflicti157S → T in AAA25972 (PubMed:3089876).Curated1
Sequence conflicti359 – 360EV → DL in AAA25972 (PubMed:3089876).Curated2

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
M14037 Genomic DNA Translation: AAA25972.1
AE004091 Genomic DNA Translation: AAG06939.1

Protein sequence database of the Protein Information Resource

More...
PIRi
A38598
B83201

NCBI Reference Sequences

More...
RefSeqi
NP_252241.1, NC_002516.2
WP_003092113.1, NZ_QZGE01000001.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
AAG06939; AAG06939; PA3551

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
879142

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
pae:PA3551

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|208964.12.peg.3716

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14037 Genomic DNA Translation: AAA25972.1
AE004091 Genomic DNA Translation: AAG06939.1
PIRiA38598
B83201
RefSeqiNP_252241.1, NC_002516.2
WP_003092113.1, NZ_QZGE01000001.1

3D structure databases

SMRiP07874
ModBaseiSearch...

Proteomic databases

PaxDbiP07874
PRIDEiP07874

Genome annotation databases

EnsemblBacteriaiAAG06939; AAG06939; PA3551
GeneIDi879142
KEGGipae:PA3551
PATRICifig|208964.12.peg.3716

Organism-specific databases

PseudoCAPiPA3551

Phylogenomic databases

eggNOGiENOG4105CJT Bacteria
COG0662 LUCA
COG0836 LUCA
HOGENOMiHOG000153549
InParanoidiP07874
KOiK16011
OMAiPATFACC
PhylomeDBiP07874

Enzyme and pathway databases

UniPathwayiUPA00126;UER00423
UPA00126;UER00930
BioCyciMetaCyc:MONOMER-13384
PAER208964:G1FZ6-3619-MONOMER
BRENDAi2.7.7.13 5087
SABIO-RKiP07874

Family and domain databases

Gene3Di2.60.120.10, 1 hit
3.90.550.10, 1 hit
InterProiView protein in InterPro
IPR006375 Man1P_GuaTrfase/Man6P_Isoase
IPR001538 Man6P_isomerase-2_C
IPR005835 NTP_transferase_dom
IPR029044 Nucleotide-diphossugar_trans
IPR014710 RmlC-like_jellyroll
IPR011051 RmlC_Cupin_sf
PfamiView protein in Pfam
PF01050 MannoseP_isomer, 1 hit
PF00483 NTP_transferase, 1 hit
SUPFAMiSSF51182 SSF51182, 1 hit
SSF53448 SSF53448, 1 hit
TIGRFAMsiTIGR01479 GMP_PMI, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiALGA_PSEAE
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P07874
Secondary accession number(s): Q9HY66
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: January 11, 2001
Last modified: December 11, 2019
This is version 143 of the entry and version 3 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
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