UniProtKB - P07866 (LTE1_YEAST)
Guanine nucleotide exchange factor LTE1
LTE1
Functioni
GDP-GTP exchange factor for TEM1, a Ras-like protein, component of the mitotic exit network (MEN). Activation of TEM1 by LTE1 in the bud ultimately leads to activation of CDC15 followed by the release of CDC14 from the nucleolus, which then inactivates cyclin-dependent kinases (CDKs) activity by several mechanism. Required for TEM1 localization to the bud cortex during mitotic exit. Fine-tunes the timing of the mitotic exit and couples this event with cytokinesis.
Involved in proprotein-processing like proalpha factor-processing in the secretory pathway.
Miscellaneous
GO - Molecular functioni
- guanyl-nucleotide exchange factor activity Source: SGD
GO - Biological processi
- asymmetric protein localization to new mitotic spindle pole body Source: SGD
- cell cycle Source: UniProtKB-KW
- cell division Source: UniProtKB-KW
- positive regulation of exit from mitosis Source: SGD
- positive regulation of GTPase activity Source: GO_Central
- Ras protein signal transduction Source: GO_Central
- vesicle-mediated transport Source: SGD
Keywordsi
Molecular function | Guanine-nucleotide releasing factor |
Biological process | Cell cycle, Cell division, Mitosis |
Enzyme and pathway databases
Reactomei | R-SCE-354192, Integrin signaling R-SCE-392517, Rap1 signalling R-SCE-416482, G alpha (12/13) signalling events R-SCE-8980692, RHOA GTPase cycle R-SCE-9013148, CDC42 GTPase cycle |
Names & Taxonomyi
Protein namesi | Recommended name: Guanine nucleotide exchange factor LTE1Alternative name(s): Low temperature essential protein 1 |
Gene namesi | Name:LTE1 Synonyms:EIS4, MSI2 Ordered Locus Names:YAL024C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000000022, LTE1 |
VEuPathDBi | FungiDB:YAL024C |
Subcellular locationi
Plasma Membrane
- plasma membrane Source: GO_Central
Other locations
- cellular bud Source: SGD
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000068884 | 1 – 1435 | Guanine nucleotide exchange factor LTE1Add BLAST | 1435 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 271 | PhosphoserineCombined sources | 1 | |
Modified residuei | 559 | PhosphoserineCombined sources | 1 | |
Modified residuei | 689 | PhosphoserineCombined sources | 1 | |
Modified residuei | 691 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 808 | PhosphoserineCombined sources | 1 | |
Modified residuei | 810 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1028 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1109 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P07866 |
PaxDbi | P07866 |
PRIDEi | P07866 |
PTM databases
CarbonylDBi | P07866 |
iPTMneti | P07866 |
Interactioni
Subunit structurei
Interacts with CDC24, CDC42, KEL1, KEL2, RAS2 and TEM1.
3 PublicationsBinary interactionsi
Protein-protein interaction databases
BioGRIDi | 31742, 601 interactors |
DIPi | DIP-337N |
IntActi | P07866, 58 interactors |
MINTi | P07866 |
STRINGi | 4932.YAL024C |
Miscellaneous databases
RNActi | P07866, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 25 – 157 | N-terminal Ras-GEFPROSITE-ProRule annotationAdd BLAST | 133 | |
Domaini | 1194 – 1434 | Ras-GEFPROSITE-ProRule annotationAdd BLAST | 241 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 235 – 256 | DisorderedSequence analysisAdd BLAST | 22 | |
Regioni | 338 – 365 | DisorderedSequence analysisAdd BLAST | 28 | |
Regioni | 582 – 689 | DisorderedSequence analysisAdd BLAST | 108 | |
Regioni | 723 – 747 | DisorderedSequence analysisAdd BLAST | 25 | |
Regioni | 851 – 871 | DisorderedSequence analysisAdd BLAST | 21 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 582 – 603 | Basic and acidic residuesSequence analysisAdd BLAST | 22 | |
Compositional biasi | 604 – 636 | Polar residuesSequence analysisAdd BLAST | 33 | |
Compositional biasi | 657 – 671 | Polar residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 723 – 744 | Basic and acidic residuesSequence analysisAdd BLAST | 22 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3417, Eukaryota |
HOGENOMi | CLU_004883_0_0_1 |
InParanoidi | P07866 |
OMAi | PFILMYD |
Family and domain databases
CDDi | cd00155, RasGEF, 1 hit cd06224, REM, 1 hit |
Gene3Di | 1.10.840.10, 1 hit |
InterProi | View protein in InterPro IPR008937, Ras-like_GEF IPR000651, Ras-like_Gua-exchang_fac_N IPR019804, Ras_G-nucl-exch_fac_CS IPR023578, Ras_GEF_dom_sf IPR001895, RASGEF_cat_dom IPR036964, RASGEF_cat_dom_sf |
PANTHERi | PTHR23113, PTHR23113, 1 hit |
Pfami | View protein in Pfam PF00617, RasGEF, 1 hit PF00618, RasGEF_N, 1 hit |
SMARTi | View protein in SMART SM00147, RasGEF, 1 hit SM00229, RasGEFN, 1 hit |
SUPFAMi | SSF48366, SSF48366, 1 hit |
PROSITEi | View protein in PROSITE PS00720, RASGEF, 1 hit PS50009, RASGEF_CAT, 1 hit PS50212, RASGEF_NTER, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MEIFSQKDYY PTPSSNVISY ESDCVSKPVN SADLPALIVH LSSPLEGVDY
60 70 80 90 100
NASADFFLIY RNFITPQDLH DLLIYRFRWC IREITTNAAK AKRRRIGEVA
110 120 130 140 150
LVRTFVLLRH SILNYFVQDF LPNITLRLRL IEFLNDKHIE QYPKIISSCI
160 170 180 190 200
INLKKNWVHC SKLVWENIEL NEPDKLDFDA WLHYSLKDFT QLESLHKRGS
210 220 230 240 250
RLSIYARQSF ASPDFRNQSV LSLYKTSDVF RLPEKLQSSN SSKNQRSPSM
260 270 280 290 300
LLFPDNTSNV YSKHRIAKEP SVDNESEDMS DSKQKISHLS KVTLVSTLMK
310 320 330 340 350
GVDYPSSYAV DKIMPPTPAK KVEFILNSLY IPEDLNEQSG TLQGTSTTSS
360 370 380 390 400
LDNNSNSNSR SNTSSMSVLH RSAIGLLAKW MKNHNRHDSS NDKKFMSAIK
410 420 430 440 450
PANQKPEMDA FVKYVVSISS LNRKSSKEEE EEFLNSDSSK FDILSARTID
460 470 480 490 500
EVESLLHLQN QLIEKVQTHS NNNRGPTVNV DCERREHIHD IKILQQNSFK
510 520 530 540 550
PSNDNFSAMD NLDLYQTVSS IAQSVISLTN TLNKQLQNNE SNMQPSPSYD
560 570 580 590 600
ALQRRKVKSL TTAYYNKMHG SYSAESMRLF DKDNSSSRTD ENGPQRLLFH
610 620 630 640 650
ETDKTNSEAI TNMTPRRKNH SQSQKSMTSS PLKNVLPDLK ESSPLNDSRE
660 670 680 690 700
DTESITYSYD SELSSSSPPR DTVTKKSRKV RNIVNNTDSP TLKTKTGFLN
710 720 730 740 750
LREFTFEDTK SLDEKKSTID GLEKNYDNKE NQESEYESTK KLDNSLDASS
760 770 780 790 800
EANNYDITTR KKHSSCNHKI KQAVVRPASG RISISRVQSI AITPTKELSI
810 820 830 840 850
VDPEQNKSNS VIEEISEIEP LNLEYNKKSA LYSDTSSTVI SISTSKLFES
860 870 880 890 900
AQNSPLKQTQ NPQREFPNGT SVSETNRIRL SIAPTIESVV SDLNSITTGS
910 920 930 940 950
TVETFETSRD LPVPHQRIIN LREEYQRGNQ DIISNTSSLH ELKTIDLSDS
960 970 980 990 1000
NNDLESPSTH AKNNKYFFSP DDGSIDVASP MKNVEELKSK FLKNESETNS
1010 1020 1030 1040 1050
NISGSVLTMD DIDINDTSSA RNTRRANSES AFTGSLNKKN LNEIANMLDD
1060 1070 1080 1090 1100
SINDDPITVA LMKLEGTYEK IPEKPENTKS SDAIGIKTSK LADEVEMLNL
1110 1120 1130 1140 1150
NNLPSFQNSP AEKRKSLLIE RRRQTIMNIP FTPDQSEKEG FTSSSPEKID
1160 1170 1180 1190 1200
VSANVDVAVQ AAQIQELIGQ YRIHDSRLMI SNNESHVPFI LMYDSLSVAQ
1210 1220 1230 1240 1250
QMTLIEKEIL GEIDWKDLLD LKMKHEGPQV ISWLQLLVRN ETLSGIDLAI
1260 1270 1280 1290 1300
SRFNLTVDWI ISEILLTKSS KMKRNVIQRF IHVADHCRTF QNFNTLMEII
1310 1320 1330 1340 1350
LALSSSVVQK FTDAWRLIEP GDLLTWEELK KIPSLDRNYS TIRNLLNSVN
1360 1370 1380 1390 1400
PLVGCVPFIV VYLSDLSANA EKKDWILEDK VVNYNKFDTN VQIVKNFIQR
1410 1420 1430
VQWSKFYTFK VNHELLSKCV YISTLTQEEI NELST
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 998 – 1009 | TNSNI…SVLTM → LIVHIRKCIDN in BAA04820 (PubMed:7941731).CuratedAdd BLAST | 12 | |
Sequence conflicti | 1161 – 1163 | AAQ → GE in AAA34746 (PubMed:3332963).Curated | 3 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L20125 Genomic DNA Translation: AAA50468.1 U12980 Genomic DNA Translation: AAC05008.1 D21354 Genomic DNA Translation: BAA04820.1 M16076 Genomic DNA Translation: AAA34746.1 J03852 Genomic DNA Translation: AAA34751.1 BK006935 Genomic DNA Translation: DAA06964.1 |
PIRi | S51997, BVBYL1 |
RefSeqi | NP_009378.1, NM_001178169.1 |
Genome annotation databases
EnsemblFungii | YAL024C_mRNA; YAL024C; YAL024C |
GeneIDi | 851209 |
KEGGi | sce:YAL024C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L20125 Genomic DNA Translation: AAA50468.1 U12980 Genomic DNA Translation: AAC05008.1 D21354 Genomic DNA Translation: BAA04820.1 M16076 Genomic DNA Translation: AAA34746.1 J03852 Genomic DNA Translation: AAA34751.1 BK006935 Genomic DNA Translation: DAA06964.1 |
PIRi | S51997, BVBYL1 |
RefSeqi | NP_009378.1, NM_001178169.1 |
3D structure databases
AlphaFoldDBi | P07866 |
SMRi | P07866 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 31742, 601 interactors |
DIPi | DIP-337N |
IntActi | P07866, 58 interactors |
MINTi | P07866 |
STRINGi | 4932.YAL024C |
PTM databases
CarbonylDBi | P07866 |
iPTMneti | P07866 |
Proteomic databases
MaxQBi | P07866 |
PaxDbi | P07866 |
PRIDEi | P07866 |
Genome annotation databases
EnsemblFungii | YAL024C_mRNA; YAL024C; YAL024C |
GeneIDi | 851209 |
KEGGi | sce:YAL024C |
Organism-specific databases
SGDi | S000000022, LTE1 |
VEuPathDBi | FungiDB:YAL024C |
Phylogenomic databases
eggNOGi | KOG3417, Eukaryota |
HOGENOMi | CLU_004883_0_0_1 |
InParanoidi | P07866 |
OMAi | PFILMYD |
Enzyme and pathway databases
Reactomei | R-SCE-354192, Integrin signaling R-SCE-392517, Rap1 signalling R-SCE-416482, G alpha (12/13) signalling events R-SCE-8980692, RHOA GTPase cycle R-SCE-9013148, CDC42 GTPase cycle |
Miscellaneous databases
PROi | PR:P07866 |
RNActi | P07866, protein |
Family and domain databases
CDDi | cd00155, RasGEF, 1 hit cd06224, REM, 1 hit |
Gene3Di | 1.10.840.10, 1 hit |
InterProi | View protein in InterPro IPR008937, Ras-like_GEF IPR000651, Ras-like_Gua-exchang_fac_N IPR019804, Ras_G-nucl-exch_fac_CS IPR023578, Ras_GEF_dom_sf IPR001895, RASGEF_cat_dom IPR036964, RASGEF_cat_dom_sf |
PANTHERi | PTHR23113, PTHR23113, 1 hit |
Pfami | View protein in Pfam PF00617, RasGEF, 1 hit PF00618, RasGEF_N, 1 hit |
SMARTi | View protein in SMART SM00147, RasGEF, 1 hit SM00229, RasGEFN, 1 hit |
SUPFAMi | SSF48366, SSF48366, 1 hit |
PROSITEi | View protein in PROSITE PS00720, RASGEF, 1 hit PS50009, RASGEF_CAT, 1 hit PS50212, RASGEF_NTER, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | LTE1_YEAST | |
Accessioni | P07866Primary (citable) accession number: P07866 Secondary accession number(s): D6VPJ4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1988 |
Last sequence update: | October 1, 1994 | |
Last modified: | May 25, 2022 | |
This is version 198 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome I
Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names - SIMILARITY comments
Index of protein domains and families