LTE1

Functionⁱ

GDP-GTP exchange factor for TEM1, a Ras-like protein, component of the mitotic exit network (MEN). Activation of TEM1 by LTE1 in the bud ultimately leads to activation of CDC15 followed by the release of CDC14 from the nucleolus, which then inactivates cyclin-dependent kinases (CDKs) activity by several mechanism. Required for TEM1 localization to the bud cortex during mitotic exit. Fine-tunes the timing of the mitotic exit and couples this event with cytokinesis.

Involved in proprotein-processing like proalpha factor-processing in the secretory pathway.

Miscellaneous

Present with 304 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functionⁱ

Ras guanyl-nucleotide exchange factor activity
Source: SGD
Inferred from sequence or structural similarityⁱ
- 7985422

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

asymmetric protein localization to new mitotic spindle pole body
Source: SGD
Inferred from mutant phenotypeⁱ
- 19948498
cell division Source: UniProtKB-KW
mitotic spindle orientation checkpoint
Source: SGD
Traceable author statementⁱ
- 16039591
regulation of exit from mitosis
Source: SGD
Traceable author statementⁱ
- 16039591
- 10219244
small GTPase mediated signal transduction Source: InterPro
vesicle-mediated transport
Source: SGD
Inferred from genetic interactionⁱ
- 17121813

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Guanine-nucleotide releasing factor
Biological process	Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

BioCycⁱ	YEAST:G3O-28835-MONOMER

BioCycⁱ

YEAST:G3O-28835-MONOMER

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Guanine nucleotide exchange factor LTE1 Alternative name(s): Low temperature essential protein 1
Gene namesⁱ	Name:LTE1 Synonyms:EIS4, MSI2 Ordered Locus Names:YAL024C
Organismⁱ	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifierⁱ	559292 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae
Proteomesⁱ	UP000002311 Componentⁱ: Chromosome I

Organism-specific databases

EuPathDBⁱ	FungiDB:YAL024C
Saccharomyces Genome Database More...SGDⁱ	S000000022 LTE1

Keywords - Cellular componentⁱ

Cytoplasm

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000068884	1 – 1435	Guanine nucleotide exchange factor LTE1Add BLAST		1435

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	271	PhosphoserineCombined sources	1
Modified residueⁱ	559	PhosphoserineCombined sources	1
Modified residueⁱ	689	PhosphoserineCombined sources	1
Modified residueⁱ	691	PhosphothreonineCombined sources	1
Modified residueⁱ	808	PhosphoserineCombined sources	1
Modified residueⁱ	810	PhosphoserineCombined sources	1
Modified residueⁱ	1028	PhosphoserineCombined sources	1
Modified residueⁱ	1109	PhosphoserineCombined sources	1

Post-translational modificationⁱ

Phosphorylated by CDC28 in a cell cycle-dependent manner and in response to nocodazole. Dephosphorylion by CDC14 triggers LTE1 release from bud cortex during the exit of mitosis.4 Publications

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P07866
PaxDbⁱ	P07866
PRIDEⁱ	P07866

PTM databases

CarbonylDBⁱ	P07866
iPTMnetⁱ	P07866

Interactionⁱ

Subunit structureⁱ

Interacts with CDC24, CDC42, KEL1, KEL2, RAS2 and TEM1.3 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
KEL1	P38853	3	EBI-10243,EBI-9619
RAS2	P01120	5	EBI-10243,EBI-14838

Show more details

GO - Molecular functionⁱ

Ras guanyl-nucleotide exchange factor activity
Source: SGD
Inferred from sequence or structural similarityⁱ
- 7985422

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	31742, 596 interactors
Database of interacting proteins More...DIPⁱ	DIP-337N
IntActⁱ	P07866, 58 interactors
Molecular INTeraction database More...MINTⁱ	P07866
STRINGⁱ	4932.YAL024C

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P07866
SMRⁱ	P07866
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	25 – 157	N-terminal Ras-GEFPROSITE-ProRule annotationAdd BLAST		133
Domainⁱ	1194 – 1434	Ras-GEFPROSITE-ProRule annotationAdd BLAST		241

Sequence similaritiesⁱ

Belongs to the LTE1 family.Curated

Phylogenomic databases

HOGENOMⁱ	HOG000066134
InParanoidⁱ	P07866
KEGG Orthology (KO) More...KOⁱ	K06681
OMAⁱ	FILMYDS
Database of Orthologous Groups More...OrthoDBⁱ	EOG092C043A

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00155 RasGEF, 1 hit cd06224 REM, 1 hit
Gene3Dⁱ	1.10.840.10, 1 hit
InterProⁱ	View protein in InterPro IPR000651 Ras-like_Gua-exchang_fac_N IPR019804 Ras_G-nucl-exch_fac_CS IPR023578 Ras_GEF_dom_sf IPR001895 RASGEF_cat_dom IPR036964 RASGEF_cat_dom_sf
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00617 RasGEF, 1 hit PF00618 RasGEF_N, 1 hit
SMARTⁱ	View protein in SMART SM00147 RasGEF, 1 hit SM00229 RasGEFN, 1 hit
SUPFAMⁱ	SSF48366 SSF48366, 2 hits
PROSITEⁱ	View protein in PROSITE PS00720 RASGEF, 1 hit PS50009 RASGEF_CAT, 1 hit PS50212 RASGEF_NTER, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P07866-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MEIFSQKDYY PTPSSNVISY ESDCVSKPVN SADLPALIVH LSSPLEGVDY 
        60         70         80         90        100
NASADFFLIY RNFITPQDLH DLLIYRFRWC IREITTNAAK AKRRRIGEVA 
       110        120        130        140        150
LVRTFVLLRH SILNYFVQDF LPNITLRLRL IEFLNDKHIE QYPKIISSCI 
       160        170        180        190        200
INLKKNWVHC SKLVWENIEL NEPDKLDFDA WLHYSLKDFT QLESLHKRGS 
       210        220        230        240        250
RLSIYARQSF ASPDFRNQSV LSLYKTSDVF RLPEKLQSSN SSKNQRSPSM 
       260        270        280        290        300
LLFPDNTSNV YSKHRIAKEP SVDNESEDMS DSKQKISHLS KVTLVSTLMK 
       310        320        330        340        350
GVDYPSSYAV DKIMPPTPAK KVEFILNSLY IPEDLNEQSG TLQGTSTTSS 
       360        370        380        390        400
LDNNSNSNSR SNTSSMSVLH RSAIGLLAKW MKNHNRHDSS NDKKFMSAIK 
       410        420        430        440        450
PANQKPEMDA FVKYVVSISS LNRKSSKEEE EEFLNSDSSK FDILSARTID 
       460        470        480        490        500
EVESLLHLQN QLIEKVQTHS NNNRGPTVNV DCERREHIHD IKILQQNSFK 
       510        520        530        540        550
PSNDNFSAMD NLDLYQTVSS IAQSVISLTN TLNKQLQNNE SNMQPSPSYD 
       560        570        580        590        600
ALQRRKVKSL TTAYYNKMHG SYSAESMRLF DKDNSSSRTD ENGPQRLLFH 
       610        620        630        640        650
ETDKTNSEAI TNMTPRRKNH SQSQKSMTSS PLKNVLPDLK ESSPLNDSRE 
       660        670        680        690        700
DTESITYSYD SELSSSSPPR DTVTKKSRKV RNIVNNTDSP TLKTKTGFLN 
       710        720        730        740        750
LREFTFEDTK SLDEKKSTID GLEKNYDNKE NQESEYESTK KLDNSLDASS 
       760        770        780        790        800
EANNYDITTR KKHSSCNHKI KQAVVRPASG RISISRVQSI AITPTKELSI 
       810        820        830        840        850
VDPEQNKSNS VIEEISEIEP LNLEYNKKSA LYSDTSSTVI SISTSKLFES 
       860        870        880        890        900
AQNSPLKQTQ NPQREFPNGT SVSETNRIRL SIAPTIESVV SDLNSITTGS 
       910        920        930        940        950
TVETFETSRD LPVPHQRIIN LREEYQRGNQ DIISNTSSLH ELKTIDLSDS 
       960        970        980        990       1000
NNDLESPSTH AKNNKYFFSP DDGSIDVASP MKNVEELKSK FLKNESETNS 
      1010       1020       1030       1040       1050
NISGSVLTMD DIDINDTSSA RNTRRANSES AFTGSLNKKN LNEIANMLDD 
      1060       1070       1080       1090       1100
SINDDPITVA LMKLEGTYEK IPEKPENTKS SDAIGIKTSK LADEVEMLNL 
      1110       1120       1130       1140       1150
NNLPSFQNSP AEKRKSLLIE RRRQTIMNIP FTPDQSEKEG FTSSSPEKID 
      1160       1170       1180       1190       1200
VSANVDVAVQ AAQIQELIGQ YRIHDSRLMI SNNESHVPFI LMYDSLSVAQ 
      1210       1220       1230       1240       1250
QMTLIEKEIL GEIDWKDLLD LKMKHEGPQV ISWLQLLVRN ETLSGIDLAI 
      1260       1270       1280       1290       1300
SRFNLTVDWI ISEILLTKSS KMKRNVIQRF IHVADHCRTF QNFNTLMEII 
      1310       1320       1330       1340       1350
LALSSSVVQK FTDAWRLIEP GDLLTWEELK KIPSLDRNYS TIRNLLNSVN 
      1360       1370       1380       1390       1400
PLVGCVPFIV VYLSDLSANA EKKDWILEDK VVNYNKFDTN VQIVKNFIQR 
      1410       1420       1430 
VQWSKFYTFK VNHELLSKCV YISTLTQEEI NELST

Length:1,435

Mass (Da):163,150

Last modified:October 1, 1994 - v2

Checksum:ⁱEED7E5150BECA3DE

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	998 – 1009	TNSNI…SVLTM → LIVHIRKCIDN in BAA04820 (PubMed:7941731).CuratedAdd BLAST		12
Sequence conflictⁱ	1161 – 1163	AAQ → GE in AAA34746 (PubMed:3332963).Curated		3

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	L20125 Genomic DNA Translation: AAA50468.1 U12980 Genomic DNA Translation: AAC05008.1 D21354 Genomic DNA Translation: BAA04820.1 M16076 Genomic DNA Translation: AAA34746.1 J03852 Genomic DNA Translation: AAA34751.1 BK006935 Genomic DNA Translation: DAA06964.1
PIRⁱ	S51997 BVBYL1
NCBI Reference Sequences More...RefSeqⁱ	NP_009378.1, NM_001178169.1

Genome annotation databases

EnsemblFungiⁱ	YAL024C; YAL024C; YAL024C
GeneIDⁱ	851209
KEGGⁱ	sce:YAL024C

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length
P07866	Low temperature essential protein		YEAS1		1435
LTE1p Protein like GDP/GTP exchange factors		Saccharomyces sp. 'boulardii'		1435
Lte1p		YEASC		1435
K7_Lte1p		YEASK		1435
Lte1p		YEAS8		1435
+4

Protein	Similar proteins		Species	Score	Length
P07866	Low temperature essential protein		YEAS1		1435
LTE1p Protein like GDP/GTP exchange factors		Saccharomyces sp. 'boulardii'		1435
Lte1p		YEASC		1435
K7_Lte1p		YEASK		1435
Low temperature essential protein		YEAS7		1435
+7

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	L20125 Genomic DNA Translation: AAA50468.1 U12980 Genomic DNA Translation: AAC05008.1 D21354 Genomic DNA Translation: BAA04820.1 M16076 Genomic DNA Translation: AAA34746.1 J03852 Genomic DNA Translation: AAA34751.1 BK006935 Genomic DNA Translation: DAA06964.1
PIRⁱ	S51997 BVBYL1
NCBI Reference Sequences More...RefSeqⁱ	NP_009378.1, NM_001178169.1

3D structure databases

ProteinModelPortalⁱ	P07866
SMRⁱ	P07866
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	31742, 596 interactors
Database of interacting proteins More...DIPⁱ	DIP-337N
IntActⁱ	P07866, 58 interactors
Molecular INTeraction database More...MINTⁱ	P07866
STRINGⁱ	4932.YAL024C

PTM databases

CarbonylDBⁱ	P07866
iPTMnetⁱ	P07866

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P07866
PaxDbⁱ	P07866
PRIDEⁱ	P07866

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblFungiⁱ	YAL024C; YAL024C; YAL024C
GeneIDⁱ	851209
KEGGⁱ	sce:YAL024C

Organism-specific databases

EuPathDBⁱ	FungiDB:YAL024C
Saccharomyces Genome Database More...SGDⁱ	S000000022 LTE1

Phylogenomic databases

HOGENOMⁱ	HOG000066134
InParanoidⁱ	P07866
KEGG Orthology (KO) More...KOⁱ	K06681
OMAⁱ	FILMYDS
Database of Orthologous Groups More...OrthoDBⁱ	EOG092C043A

Enzyme and pathway databases

BioCycⁱ	YEAST:G3O-28835-MONOMER

BioCycⁱ

YEAST:G3O-28835-MONOMER

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P07866

PROⁱ

PR:P07866

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00155 RasGEF, 1 hit cd06224 REM, 1 hit
Gene3Dⁱ	1.10.840.10, 1 hit
InterProⁱ	View protein in InterPro IPR000651 Ras-like_Gua-exchang_fac_N IPR019804 Ras_G-nucl-exch_fac_CS IPR023578 Ras_GEF_dom_sf IPR001895 RASGEF_cat_dom IPR036964 RASGEF_cat_dom_sf
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00617 RasGEF, 1 hit PF00618 RasGEF_N, 1 hit
SMARTⁱ	View protein in SMART SM00147 RasGEF, 1 hit SM00229 RasGEFN, 1 hit
SUPFAMⁱ	SSF48366 SSF48366, 2 hits
PROSITEⁱ	View protein in PROSITE PS00720 RASGEF, 1 hit PS50009 RASGEF_CAT, 1 hit PS50212 RASGEF_NTER, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	LTE1_YEAST
Accessionⁱ	P07866Primary (citable) accession number: P07866 Secondary accession number(s): D6VPJ4
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
	Last sequence update:	October 1, 1994
	Last modified:	June 20, 2018
	This is version 177 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Fungal Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
Yeast chromosome I
Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

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UniProtKB - P07866 (LTE1_YEAST)

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Guanine nucleotide exchange factor LTE1

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

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Other locations

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Proteomic databases

PTM databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

PTM / Processingⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ