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Protein

Guanine nucleotide exchange factor LTE1

Gene

LTE1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

GDP-GTP exchange factor for TEM1, a Ras-like protein, component of the mitotic exit network (MEN). Activation of TEM1 by LTE1 in the bud ultimately leads to activation of CDC15 followed by the release of CDC14 from the nucleolus, which then inactivates cyclin-dependent kinases (CDKs) activity by several mechanism. Required for TEM1 localization to the bud cortex during mitotic exit. Fine-tunes the timing of the mitotic exit and couples this event with cytokinesis.
Involved in proprotein-processing like proalpha factor-processing in the secretory pathway.

Miscellaneous

Present with 304 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • Ras guanyl-nucleotide exchange factor activity Source: SGD

GO - Biological processi

  • asymmetric protein localization to new mitotic spindle pole body Source: SGD
  • cell division Source: UniProtKB-KW
  • mitotic spindle orientation checkpoint Source: SGD
  • regulation of exit from mitosis Source: SGD
  • small GTPase mediated signal transduction Source: InterPro
  • vesicle-mediated transport Source: SGD

Keywordsi

Molecular functionGuanine-nucleotide releasing factor
Biological processCell cycle, Cell division, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-28835-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide exchange factor LTE1
Alternative name(s):
Low temperature essential protein 1
Gene namesi
Name:LTE1
Synonyms:EIS4, MSI2
Ordered Locus Names:YAL024C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome I

Organism-specific databases

SGDiS000000022 LTE1

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000688841 – 1435Guanine nucleotide exchange factor LTE1Add BLAST1435

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei271PhosphoserineCombined sources1
Modified residuei559PhosphoserineCombined sources1
Modified residuei689PhosphoserineCombined sources1
Modified residuei691PhosphothreonineCombined sources1
Modified residuei808PhosphoserineCombined sources1
Modified residuei810PhosphoserineCombined sources1
Modified residuei1028PhosphoserineCombined sources1
Modified residuei1109PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated by CDC28 in a cell cycle-dependent manner and in response to nocodazole. Dephosphorylion by CDC14 triggers LTE1 release from bud cortex during the exit of mitosis.4 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP07866
PaxDbiP07866
PRIDEiP07866

PTM databases

CarbonylDBiP07866
iPTMnetiP07866

Interactioni

Subunit structurei

Interacts with CDC24, CDC42, KEL1, KEL2, RAS2 and TEM1.3 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi31742, 596 interactors
DIPiDIP-337N
IntActiP07866, 58 interactors
MINTiP07866
STRINGi4932.YAL024C

Structurei

3D structure databases

ProteinModelPortaliP07866
SMRiP07866
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 157N-terminal Ras-GEFPROSITE-ProRule annotationAdd BLAST133
Domaini1194 – 1434Ras-GEFPROSITE-ProRule annotationAdd BLAST241

Sequence similaritiesi

Belongs to the LTE1 family.Curated

Phylogenomic databases

HOGENOMiHOG000066134
InParanoidiP07866
KOiK06681
OMAiPFILMYD
OrthoDBiEOG092C043A

Family and domain databases

CDDicd00155 RasGEF, 1 hit
cd06224 REM, 1 hit
Gene3Di1.10.840.10, 1 hit
InterProiView protein in InterPro
IPR030704 Lte1
IPR008937 Ras-like_GEF
IPR000651 Ras-like_Gua-exchang_fac_N
IPR019804 Ras_G-nucl-exch_fac_CS
IPR023578 Ras_GEF_dom_sf
IPR001895 RASGEF_cat_dom
IPR036964 RASGEF_cat_dom_sf
PANTHERiPTHR23113 PTHR23113, 2 hits
PTHR23113:SF244 PTHR23113:SF244, 2 hits
PfamiView protein in Pfam
PF00617 RasGEF, 1 hit
PF00618 RasGEF_N, 1 hit
SMARTiView protein in SMART
SM00147 RasGEF, 1 hit
SM00229 RasGEFN, 1 hit
SUPFAMiSSF48366 SSF48366, 1 hit
PROSITEiView protein in PROSITE
PS00720 RASGEF, 1 hit
PS50009 RASGEF_CAT, 1 hit
PS50212 RASGEF_NTER, 1 hit

Sequencei

Sequence statusi: Complete.

P07866-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEIFSQKDYY PTPSSNVISY ESDCVSKPVN SADLPALIVH LSSPLEGVDY
60 70 80 90 100
NASADFFLIY RNFITPQDLH DLLIYRFRWC IREITTNAAK AKRRRIGEVA
110 120 130 140 150
LVRTFVLLRH SILNYFVQDF LPNITLRLRL IEFLNDKHIE QYPKIISSCI
160 170 180 190 200
INLKKNWVHC SKLVWENIEL NEPDKLDFDA WLHYSLKDFT QLESLHKRGS
210 220 230 240 250
RLSIYARQSF ASPDFRNQSV LSLYKTSDVF RLPEKLQSSN SSKNQRSPSM
260 270 280 290 300
LLFPDNTSNV YSKHRIAKEP SVDNESEDMS DSKQKISHLS KVTLVSTLMK
310 320 330 340 350
GVDYPSSYAV DKIMPPTPAK KVEFILNSLY IPEDLNEQSG TLQGTSTTSS
360 370 380 390 400
LDNNSNSNSR SNTSSMSVLH RSAIGLLAKW MKNHNRHDSS NDKKFMSAIK
410 420 430 440 450
PANQKPEMDA FVKYVVSISS LNRKSSKEEE EEFLNSDSSK FDILSARTID
460 470 480 490 500
EVESLLHLQN QLIEKVQTHS NNNRGPTVNV DCERREHIHD IKILQQNSFK
510 520 530 540 550
PSNDNFSAMD NLDLYQTVSS IAQSVISLTN TLNKQLQNNE SNMQPSPSYD
560 570 580 590 600
ALQRRKVKSL TTAYYNKMHG SYSAESMRLF DKDNSSSRTD ENGPQRLLFH
610 620 630 640 650
ETDKTNSEAI TNMTPRRKNH SQSQKSMTSS PLKNVLPDLK ESSPLNDSRE
660 670 680 690 700
DTESITYSYD SELSSSSPPR DTVTKKSRKV RNIVNNTDSP TLKTKTGFLN
710 720 730 740 750
LREFTFEDTK SLDEKKSTID GLEKNYDNKE NQESEYESTK KLDNSLDASS
760 770 780 790 800
EANNYDITTR KKHSSCNHKI KQAVVRPASG RISISRVQSI AITPTKELSI
810 820 830 840 850
VDPEQNKSNS VIEEISEIEP LNLEYNKKSA LYSDTSSTVI SISTSKLFES
860 870 880 890 900
AQNSPLKQTQ NPQREFPNGT SVSETNRIRL SIAPTIESVV SDLNSITTGS
910 920 930 940 950
TVETFETSRD LPVPHQRIIN LREEYQRGNQ DIISNTSSLH ELKTIDLSDS
960 970 980 990 1000
NNDLESPSTH AKNNKYFFSP DDGSIDVASP MKNVEELKSK FLKNESETNS
1010 1020 1030 1040 1050
NISGSVLTMD DIDINDTSSA RNTRRANSES AFTGSLNKKN LNEIANMLDD
1060 1070 1080 1090 1100
SINDDPITVA LMKLEGTYEK IPEKPENTKS SDAIGIKTSK LADEVEMLNL
1110 1120 1130 1140 1150
NNLPSFQNSP AEKRKSLLIE RRRQTIMNIP FTPDQSEKEG FTSSSPEKID
1160 1170 1180 1190 1200
VSANVDVAVQ AAQIQELIGQ YRIHDSRLMI SNNESHVPFI LMYDSLSVAQ
1210 1220 1230 1240 1250
QMTLIEKEIL GEIDWKDLLD LKMKHEGPQV ISWLQLLVRN ETLSGIDLAI
1260 1270 1280 1290 1300
SRFNLTVDWI ISEILLTKSS KMKRNVIQRF IHVADHCRTF QNFNTLMEII
1310 1320 1330 1340 1350
LALSSSVVQK FTDAWRLIEP GDLLTWEELK KIPSLDRNYS TIRNLLNSVN
1360 1370 1380 1390 1400
PLVGCVPFIV VYLSDLSANA EKKDWILEDK VVNYNKFDTN VQIVKNFIQR
1410 1420 1430
VQWSKFYTFK VNHELLSKCV YISTLTQEEI NELST
Length:1,435
Mass (Da):163,150
Last modified:October 1, 1994 - v2
Checksum:iEED7E5150BECA3DE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti998 – 1009TNSNI…SVLTM → LIVHIRKCIDN in BAA04820 (PubMed:7941731).CuratedAdd BLAST12
Sequence conflicti1161 – 1163AAQ → GE in AAA34746 (PubMed:3332963).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L20125 Genomic DNA Translation: AAA50468.1
U12980 Genomic DNA Translation: AAC05008.1
D21354 Genomic DNA Translation: BAA04820.1
M16076 Genomic DNA Translation: AAA34746.1
J03852 Genomic DNA Translation: AAA34751.1
BK006935 Genomic DNA Translation: DAA06964.1
PIRiS51997 BVBYL1
RefSeqiNP_009378.1, NM_001178169.1

Genome annotation databases

EnsemblFungiiYAL024C; YAL024C; YAL024C
GeneIDi851209
KEGGisce:YAL024C

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L20125 Genomic DNA Translation: AAA50468.1
U12980 Genomic DNA Translation: AAC05008.1
D21354 Genomic DNA Translation: BAA04820.1
M16076 Genomic DNA Translation: AAA34746.1
J03852 Genomic DNA Translation: AAA34751.1
BK006935 Genomic DNA Translation: DAA06964.1
PIRiS51997 BVBYL1
RefSeqiNP_009378.1, NM_001178169.1

3D structure databases

ProteinModelPortaliP07866
SMRiP07866
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31742, 596 interactors
DIPiDIP-337N
IntActiP07866, 58 interactors
MINTiP07866
STRINGi4932.YAL024C

PTM databases

CarbonylDBiP07866
iPTMnetiP07866

Proteomic databases

MaxQBiP07866
PaxDbiP07866
PRIDEiP07866

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYAL024C; YAL024C; YAL024C
GeneIDi851209
KEGGisce:YAL024C

Organism-specific databases

SGDiS000000022 LTE1

Phylogenomic databases

HOGENOMiHOG000066134
InParanoidiP07866
KOiK06681
OMAiPFILMYD
OrthoDBiEOG092C043A

Enzyme and pathway databases

BioCyciYEAST:G3O-28835-MONOMER

Miscellaneous databases

PROiPR:P07866

Family and domain databases

CDDicd00155 RasGEF, 1 hit
cd06224 REM, 1 hit
Gene3Di1.10.840.10, 1 hit
InterProiView protein in InterPro
IPR030704 Lte1
IPR008937 Ras-like_GEF
IPR000651 Ras-like_Gua-exchang_fac_N
IPR019804 Ras_G-nucl-exch_fac_CS
IPR023578 Ras_GEF_dom_sf
IPR001895 RASGEF_cat_dom
IPR036964 RASGEF_cat_dom_sf
PANTHERiPTHR23113 PTHR23113, 2 hits
PTHR23113:SF244 PTHR23113:SF244, 2 hits
PfamiView protein in Pfam
PF00617 RasGEF, 1 hit
PF00618 RasGEF_N, 1 hit
SMARTiView protein in SMART
SM00147 RasGEF, 1 hit
SM00229 RasGEFN, 1 hit
SUPFAMiSSF48366 SSF48366, 1 hit
PROSITEiView protein in PROSITE
PS00720 RASGEF, 1 hit
PS50009 RASGEF_CAT, 1 hit
PS50212 RASGEF_NTER, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiLTE1_YEAST
AccessioniPrimary (citable) accession number: P07866
Secondary accession number(s): D6VPJ4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: October 1, 1994
Last modified: November 7, 2018
This is version 180 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome I
    Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names
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Main funding by: National Institutes of Health

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