UniProtKB - P07846 (DHSO_SHEEP)
Sorbitol dehydrogenase
SORD
Functioni
Polyol dehydrogenase that catalyzes the reversible NAD+-dependent oxidation of various sugar alcohols. Is mostly active with xylitol, L-iditol and D-sorbitol (D-glucitol) as substrates, leading to the C2-oxidized products D-xylulose, L-sorbose and D-fructose, respectively (PubMed:1459146).
Is a key enzyme in the polyol pathway that interconverts glucose and fructose via sorbitol, which constitutes an important alternate route for glucose metabolism (By similarity).
May play a role in sperm motility by using sorbitol as an alternative energy source for sperm motility (By similarity).
By similarity1 PublicationCatalytic activityi
Cofactori
Kineticsi
- KM=3.6 mM for D-sorbitol (at pH 7.4)1 Publication
- KM=0.90 mM for xylitol (at pH 7.4)1 Publication
- KM=3.3 mM for L-iditol (at pH 7.4)1 Publication
- KM=51 mM for ribitol (at pH 7.4)1 Publication
- KM=145 mM for D-mannitol (at pH 7.4)1 Publication
- KM=680 mM for galactitol (at pH 7.4)1 Publication
- KM=1.8 mM for D-sorbitol (at pH 9.9)1 Publication
- KM=0.70 mM for xylitol (at pH 9.9)1 Publication
- KM=2.5 mM for L-iditol (at pH 9.9)1 Publication
- KM=5.6 mM for ribitol (at pH 9.9)1 Publication
- KM=95 mM for D-mannitol (at pH 9.9)1 Publication
- KM=285 mM for galactitol (at pH 9.9)1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 43 | Zinc; catalytic1 Publication | 1 | |
Binding sitei | 49 | Substrate1 Publication | 1 | |
Metal bindingi | 67 | Zinc; catalytic1 Publication | 1 | |
Metal bindingi | 68 | Zinc; catalytic1 Publication | 1 | |
Binding sitei | 153 | Substrate1 Publication | 1 | |
Binding sitei | 181 | NAD; via amide nitrogenBy similarity | 1 | |
Binding sitei | 201 | NADBy similarity | 1 | |
Binding sitei | 206 | NADBy similarity | 1 | |
Binding sitei | 296 | Substrate1 Publication | 1 | |
Binding sitei | 297 | Substrate1 Publication | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 270 – 272 | NADBy similarity | 3 | |
Nucleotide bindingi | 294 – 296 | NADBy similarity | 3 |
GO - Molecular functioni
- D-xylulose reductase activity Source: UniProtKB-EC
- L-iditol 2-dehydrogenase activity Source: UniProtKB
- NAD binding Source: AgBase
- zinc ion binding Source: UniProtKB
GO - Biological processi
- flagellated sperm motility Source: UniProtKB
- fructose biosynthetic process Source: AgBase
- sorbitol catabolic process Source: AgBase
Keywordsi
Molecular function | Oxidoreductase |
Ligand | Metal-binding, NAD, Zinc |
Enzyme and pathway databases
SABIO-RKi | P07846 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:SORD |
Organismi | Ovis aries (Sheep) |
Taxonomic identifieri | 9940 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Caprinae › Ovis |
Proteomesi |
|
Subcellular locationi
Mitochondrion
- Mitochondrion membrane By similarity; Peripheral membrane protein By similarity
Other locations
- flagellum By similarity
Note: Associated with mitochondria of the midpiece and near the plasma membrane in the principal piece of the flagellum. Also found in the epididymosome, secreted by the epididymal epithelium and that transfers proteins from the epididymal fluid to the sperm surface.By similarity
Mitochondrion
- mitochondrial membrane Source: UniProtKB-SubCell
Other locations
- motile cilium Source: UniProtKB
- protein-containing complex Source: AgBase
Keywords - Cellular componenti
Cell projection, Cilium, Flagellum, Membrane, MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000160819 | 1 – 354 | Sorbitol dehydrogenaseAdd BLAST | 354 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 208 | PhosphoserineBy similarity | 1 | |
Modified residuei | 222 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinExpressioni
Tissue specificityi
Interactioni
Subunit structurei
Homotetramer.
1 PublicationProtein-protein interaction databases
STRINGi | 9940.ENSOARP00000022632 |
Structurei
Secondary structure
3D structure databases
SMRi | P07846 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P07846 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0024, Eukaryota |
Family and domain databases
CDDi | cd05285, sorbitol_DH, 1 hit |
InterProi | View protein in InterPro IPR013149, ADH_C IPR013154, ADH_N IPR002328, ADH_Zn_CS IPR011032, GroES-like_sf IPR036291, NAD(P)-bd_dom_sf IPR020843, PKS_ER IPR045306, SDH-like |
Pfami | View protein in Pfam PF08240, ADH_N, 1 hit PF00107, ADH_zinc_N, 1 hit |
SMARTi | View protein in SMART SM00829, PKS_ER, 1 hit |
SUPFAMi | SSF50129, SSF50129, 1 hit SSF51735, SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00059, ADH_ZINC, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
AKPAAENLSL VVHGPGDLRL ENYPIPEPGP NEVLLKMHSV GICGSDVHYW
60 70 80 90 100
QGRIGDFVVK KPMVLGHEAS GTVVKVGSLV RHLQPGDRVA IQPGAPRQTD
110 120 130 140 150
EFCKIGRYNL SPTIFFCATP PDDGNLCRFY KHNANFCYKL PDNVTFEEGA
160 170 180 190 200
LIEPLSVGIH ACRRAGVTLG NKVLVCGAGP IGLVNLLAAK AMGAAQVVVT
210 220 230 240 250
DLSASRLSKA KEVGADFILE ISNESPEEIA KKVEGLLGSK PEVTIECTGV
260 270 280 290 300
ETSIQAGIYA THSGGTLVLV GLGSEMTSVP LVHAATREVD IKGVFRYCNT
310 320 330 340 350
WPMAISMLAS KSVNVKPLVT HRFPLEKALE AFETSKKGLG LKVMIKCDPS
DQNP
Sequence databases
PIRi | S10065 |
Similar proteinsi
Cross-referencesi
Sequence databases
PIRi | S10065 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3QE3 | X-ray | 1.90 | A | 1-354 | [»] | |
SMRi | P07846 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 9940.ENSOARP00000022632 |
Chemistry databases
ChEMBLi | CHEMBL1075154 |
Phylogenomic databases
eggNOGi | KOG0024, Eukaryota |
Enzyme and pathway databases
SABIO-RKi | P07846 |
Miscellaneous databases
EvolutionaryTracei | P07846 |
Family and domain databases
CDDi | cd05285, sorbitol_DH, 1 hit |
InterProi | View protein in InterPro IPR013149, ADH_C IPR013154, ADH_N IPR002328, ADH_Zn_CS IPR011032, GroES-like_sf IPR036291, NAD(P)-bd_dom_sf IPR020843, PKS_ER IPR045306, SDH-like |
Pfami | View protein in Pfam PF08240, ADH_N, 1 hit PF00107, ADH_zinc_N, 1 hit |
SMARTi | View protein in SMART SM00829, PKS_ER, 1 hit |
SUPFAMi | SSF50129, SSF50129, 1 hit SSF51735, SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00059, ADH_ZINC, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DHSO_SHEEP | |
Accessioni | P07846Primary (citable) accession number: P07846 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1988 |
Last sequence update: | August 1, 1988 | |
Last modified: | February 23, 2022 | |
This is version 134 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families