UniProtKB - P07834 (CDC4_YEAST)
Protein
Cell division control protein 4
Gene
CDC4
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds to phosphorylated target proteins. Directs ubiquitination of the phosphorylated CDK inhibitor SIC1. Involved in the degradation of CDC6 together with CDC34/UBC3 and CDC53, and in restricting the degradation of FAR1 to the nucleus. Is essential for initiation of DNA replication and separation of the spindle pole bodies to form the poles of the mitotic spindle. It also plays a role in bud development, fusion of zygotic nuclei after conjugation and various aspects of sporulation. Required for HTA1-HTB1 locus transcription activation. Required for G1/S and G2/M transition.9 Publications
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
GO - Molecular functioni
- phosphoserine residue binding Source: SGD
- U3 snoRNA binding Source: GO_Central
- ubiquitin binding Source: SGD
GO - Biological processi
- cell division Source: UniProtKB-KW
- endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: GO_Central
- endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: GO_Central
- G1/S transition of mitotic cell cycle Source: SGD
- G2/M transition of mitotic cell cycle Source: SGD
- meiotic cell cycle Source: SGD
- protein ubiquitination Source: SGD
- SCF-dependent proteasomal ubiquitin-dependent protein catabolic process Source: SGD
- sporulation resulting in formation of a cellular spore Source: UniProtKB-KW
- ubiquitin-dependent protein catabolic process Source: SGD
Keywordsi
Biological process | Cell cycle, Cell division, Mitosis, Sporulation, Ubl conjugation pathway |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: Cell division control protein 4Alternative name(s): E3 ubiquitin ligase complex SCF subunit CDC4 F-box protein CDC4 |
Gene namesi | Name:CDC4 Ordered Locus Names:YFL009W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YFL009W |
SGDi | S000001885, CDC4 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Nucleus
- nuclear matrix Source: SGD
- nuclear SCF ubiquitin ligase complex Source: SGD
- nucleolus Source: GO_Central
- nucleus Source: SGD
Other locations
- 90S preribosome Source: GO_Central
- SCF ubiquitin ligase complex Source: SGD
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 82 | K → A: Prevents nuclear localization; when associated with A-83 and A-85. | 1 | |
Mutagenesisi | 83 | R → A: Prevents nuclear localization; when associated with A-82 and A-85. | 1 | |
Mutagenesisi | 83 | R → G: Prevents nuclear localization. | 1 | |
Mutagenesisi | 85 | K → A: Prevents nuclear localization; when associated with A-82 and A-83. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000050899 | 1 – 779 | Cell division control protein 4Add BLAST | 779 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 104 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P07834 |
PaxDbi | P07834 |
PRIDEi | P07834 |
PTM databases
iPTMneti | P07834 |
Interactioni
Subunit structurei
Interacts with DCD53 and SKP1.
Component of the SCF(CDC4) complex containing CDC53, SKP1, RBX1 and CDC4. CDC34.
Interacts with CDC6 and CIC1.
Interacts with SIC1; the interaction involves a SIC1 double phosphorylated motif (degron). Homodimerizes; the dimerization increases SIC1 ubiquitination in vitro.
11 PublicationsBinary interactionsi
Hide detailsP07834
GO - Molecular functioni
- phosphoserine residue binding Source: SGD
- ubiquitin binding Source: SGD
Protein-protein interaction databases
BioGRIDi | 31138, 610 interactors |
ComplexPortali | CPX-3234, SCF-Cdc4 ubiquitin ligase complex |
DIPi | DIP-1625N |
IntActi | P07834, 14 interactors |
MINTi | P07834 |
STRINGi | 4932.YFL009W |
Miscellaneous databases
RNActi | P07834, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P07834 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P07834 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 272 – 319 | F-boxPROSITE-ProRule annotationAdd BLAST | 48 | |
Repeati | 380 – 408 | WD 1Add BLAST | 29 | |
Repeati | 420 – 449 | WD 2Add BLAST | 30 | |
Repeati | 461 – 493 | WD 3Add BLAST | 33 | |
Repeati | 528 – 556 | WD 4Add BLAST | 29 | |
Repeati | 568 – 598 | WD 5Add BLAST | 31 | |
Repeati | 630 – 658 | WD 6Add BLAST | 29 | |
Repeati | 669 – 698 | WD 7Add BLAST | 30 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 82 – 85 | Nuclear localization signal | 4 |
Keywords - Domaini
Repeat, WD repeatPhylogenomic databases
eggNOGi | KOG0274, Eukaryota |
GeneTreei | ENSGT00940000174696 |
HOGENOMi | CLU_000288_103_3_1 |
InParanoidi | P07834 |
OMAi | CLQHDDE |
Family and domain databases
Gene3Di | 1.10.12.50, 1 hit 2.130.10.10, 1 hit |
InterProi | View protein in InterPro IPR031740, Cdc4_D IPR042084, Cdc4_dim_sf IPR036047, F-box-like_dom_sf IPR001810, F-box_dom IPR020472, G-protein_beta_WD-40_rep IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR019775, WD40_repeat_CS IPR017986, WD40_repeat_dom IPR036322, WD40_repeat_dom_sf |
Pfami | View protein in Pfam PF16856, CDC4_D, 1 hit PF12937, F-box-like, 1 hit PF00400, WD40, 5 hits |
PRINTSi | PR00320, GPROTEINBRPT |
SMARTi | View protein in SMART SM00256, FBOX, 1 hit SM00320, WD40, 7 hits |
SUPFAMi | SSF50978, SSF50978, 1 hit SSF81383, SSF81383, 1 hit |
PROSITEi | View protein in PROSITE PS50181, FBOX, 1 hit PS00678, WD_REPEATS_1, 4 hits PS50082, WD_REPEATS_2, 5 hits PS50294, WD_REPEATS_REGION, 1 hit |
i Sequence
Sequence statusi: Complete.
P07834-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGSFPLAEFP LRDIPVPYSY RVSGGIASSG SVTALVTAAG THRNSSTAKT
60 70 80 90 100
VETEDGEEDI DEYQRKRAAG SGESTPERSD FKRVKHDNHK TLHPVNLQNT
110 120 130 140 150
GAASVDNDGL HNLTDISNDA EKLLMSVDDG SAAPSTLSVN MGVASHNVAA
160 170 180 190 200
PTTVNAATIT GSDVSNNVNS ATINNPMEEG ALPLSPTASS PGTTTPLAKT
210 220 230 240 250
TKTINNNNNI ADLIESKDSI ISPEYLSDEI FSAINNNLPH AYFKNLLFRL
260 270 280 290 300
VANMDRSELS DLGTLIKDNL KRDLITSLPF EISLKIFNYL QFEDIINSLG
310 320 330 340 350
VSQNWNKIIR KSTSLWKKLL ISENFVSPKG FNSLNLKLSQ KYPKLSQQDR
360 370 380 390 400
LRLSFLENIF ILKNWYNPKF VPQRTTLRGH MTSVITCLQF EDNYVITGAD
410 420 430 440 450
DKMIRVYDSI NKKFLLQLSG HDGGVWALKY AHGGILVSGS TDRTVRVWDI
460 470 480 490 500
KKGCCTHVFK GHNSTVRCLD IVEYKNIKYI VTGSRDNTLH VWKLPKESSV
510 520 530 540 550
PDHGEEHDYP LVFHTPEENP YFVGVLRGHM ASVRTVSGHG NIVVSGSYDN
560 570 580 590 600
TLIVWDVAQM KCLYILSGHT DRIYSTIYDH ERKRCISASM DTTIRIWDLE
610 620 630 640 650
NIWNNGECSY ATNSASPCAK ILGAMYTLQG HTALVGLLRL SDKFLVSAAA
660 670 680 690 700
DGSIRGWDAN DYSRKFSYHH TNLSAITTFY VSDNILVSGS ENQFNIYNLR
710 720 730 740 750
SGKLVHANIL KDADQIWSVN FKGKTLVAAV EKDGQSFLEI LDFSKASKIN
760 770
YVSNPVNSSS SSLESISTSL GLTRTTIIP
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 460 | K → E in CAA29113 (PubMed:3309335).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X05625 Genomic DNA Translation: CAA29113.1 D50617 Genomic DNA Translation: BAA09229.1 Z46255 Genomic DNA Translation: CAA86341.1 BK006940 Genomic DNA Translation: DAA12431.1 |
PIRi | S56245 |
RefSeqi | NP_116585.1, NM_001179957.1 |
Genome annotation databases
EnsemblFungii | YFL009W_mRNA; YFL009W; YFL009W |
GeneIDi | 850539 |
KEGGi | sce:YFL009W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X05625 Genomic DNA Translation: CAA29113.1 D50617 Genomic DNA Translation: BAA09229.1 Z46255 Genomic DNA Translation: CAA86341.1 BK006940 Genomic DNA Translation: DAA12431.1 |
PIRi | S56245 |
RefSeqi | NP_116585.1, NM_001179957.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1NEX | X-ray | 2.70 | B/D | 263-744 | [»] | |
2P63 | X-ray | 2.67 | A/B/C/D | 222-273 | [»] | |
3MKS | X-ray | 2.60 | B/D | 263-744 | [»] | |
3V7D | X-ray | 2.31 | B/D | 263-744 | [»] | |
SMRi | P07834 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 31138, 610 interactors |
ComplexPortali | CPX-3234, SCF-Cdc4 ubiquitin ligase complex |
DIPi | DIP-1625N |
IntActi | P07834, 14 interactors |
MINTi | P07834 |
STRINGi | 4932.YFL009W |
PTM databases
iPTMneti | P07834 |
Proteomic databases
MaxQBi | P07834 |
PaxDbi | P07834 |
PRIDEi | P07834 |
Genome annotation databases
EnsemblFungii | YFL009W_mRNA; YFL009W; YFL009W |
GeneIDi | 850539 |
KEGGi | sce:YFL009W |
Organism-specific databases
EuPathDBi | FungiDB:YFL009W |
SGDi | S000001885, CDC4 |
Phylogenomic databases
eggNOGi | KOG0274, Eukaryota |
GeneTreei | ENSGT00940000174696 |
HOGENOMi | CLU_000288_103_3_1 |
InParanoidi | P07834 |
OMAi | CLQHDDE |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Miscellaneous databases
EvolutionaryTracei | P07834 |
PROi | PR:P07834 |
RNActi | P07834, protein |
Family and domain databases
Gene3Di | 1.10.12.50, 1 hit 2.130.10.10, 1 hit |
InterProi | View protein in InterPro IPR031740, Cdc4_D IPR042084, Cdc4_dim_sf IPR036047, F-box-like_dom_sf IPR001810, F-box_dom IPR020472, G-protein_beta_WD-40_rep IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR019775, WD40_repeat_CS IPR017986, WD40_repeat_dom IPR036322, WD40_repeat_dom_sf |
Pfami | View protein in Pfam PF16856, CDC4_D, 1 hit PF12937, F-box-like, 1 hit PF00400, WD40, 5 hits |
PRINTSi | PR00320, GPROTEINBRPT |
SMARTi | View protein in SMART SM00256, FBOX, 1 hit SM00320, WD40, 7 hits |
SUPFAMi | SSF50978, SSF50978, 1 hit SSF81383, SSF81383, 1 hit |
PROSITEi | View protein in PROSITE PS50181, FBOX, 1 hit PS00678, WD_REPEATS_1, 4 hits PS50082, WD_REPEATS_2, 5 hits PS50294, WD_REPEATS_REGION, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CDC4_YEAST | |
Accessioni | P07834Primary (citable) accession number: P07834 Secondary accession number(s): D6VTM1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1988 |
Last sequence update: | November 1, 1995 | |
Last modified: | December 2, 2020 | |
This is version 209 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast chromosome VI
Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD