UniProtKB - P07821 (FHUC_ECOLI)
Protein
Iron(3+)-hydroxamate import ATP-binding protein FhuC
Gene
fhuC
Organism
Escherichia coli (strain K12)
Status
Functioni
Part of the ABC transporter complex FhuCDB involved in iron3+-hydroxamate import. Responsible for energy coupling to the transport system.1 Publication
Catalytic activityi
- ATP + H(2)O + Fe(3+)-hydroxamate complex-[hydroxamate-binding protein](Side 1) = ADP + phosphate + Fe(3+)-hydroxamate complex(Side 2) + [hydroxamate-binding protein](Side 1).1 Publication EC:7.2.2.16
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 44 – 51 | ATPPROSITE-ProRule annotation | 8 | |
Nucleotide bindingi | 168 – 179 | ATPPROSITE-ProRule annotationAdd BLAST | 12 |
GO - Molecular functioni
- ABC-type ferric hydroxamate transporter activity Source: UniProtKB-EC
- ATPase activity Source: InterPro
- ATP binding Source: EcoCyc
Keywordsi
Molecular function | Translocase |
Biological process | Ion transport, Iron transport, Transport |
Ligand | ATP-binding, Iron, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:FHUC-MONOMER MetaCyc:FHUC-MONOMER |
Protein family/group databases
TCDBi | 3.A.1.14.3, the atp-binding cassette (abc) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: Iron(3+)-hydroxamate import ATP-binding protein FhuCCurated (EC:7.2.2.161 Publication)Alternative name(s): Ferric hydroxamate uptake protein CCurated Ferrichrome transport ATP-binding protein FhuCCurated Iron(III)-hydroxamate import ATP-binding protein FhuCCurated |
Gene namesi | Name:fhuC Ordered Locus Names:b0151, JW0147 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane 1 Publication; Peripheral membrane protein 1 Publication
Note: FhuB mediates the association of FhuC with the cytoplasmic membrane.1 Publication
Plasma Membrane
- ATP-binding cassette (ABC) transporter complex Source: EcoCyc
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 50 | K → Q: Lack of activity. 1 Publication | 1 | |
Mutagenesisi | 172 | D → E: Lack of activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000092327 | 1 – 265 | Iron(3+)-hydroxamate import ATP-binding protein FhuCAdd BLAST | 265 |
Proteomic databases
PaxDbi | P07821 |
PRIDEi | P07821 |
Expressioni
Inductioni
Induced 1.6-fold by hydroxyurea.1 Publication
Interactioni
Subunit structurei
The complex is composed of two ATP-binding proteins (FhuC), two transmembrane proteins (FhuB) and a solute-binding protein (FhuD) (Probable). FhuC interacts with FhuB (PubMed:1551849).
Curated1 PublicationProtein-protein interaction databases
BioGRIDi | 4261072, 353 interactors 849632, 2 interactors |
ComplexPortali | CPX-4286, Ferric-hydroxamate ABC transporter complex |
IntActi | P07821, 5 interactors |
STRINGi | 511145.b0151 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 12 – 248 | ABC transporterPROSITE-ProRule annotationAdd BLAST | 237 |
Sequence similaritiesi
Belongs to the ABC transporter superfamily. Iron (Fe3+)-hydroxamate importer (TC 3.A.1.14.7) family. [View classification]Curated
Phylogenomic databases
eggNOGi | COG1120, Bacteria |
InParanoidi | P07821 |
PhylomeDBi | P07821 |
Family and domain databases
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR003439, ABC_transporter-like IPR017871, ABC_transporter_CS IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF00005, ABC_tran, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00211, ABC_TRANSPORTER_1, 1 hit PS50893, ABC_TRANSPORTER_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P07821-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQEYTNHSDT TFALRNISFR VPGRTLLHPL SLTFPAGKVT GLIGHNGSGK
60 70 80 90 100
STLLKMLGRH QPPSEGEILL DAQPLESWSS KAFARKVAYL PQQLPPAEGM
110 120 130 140 150
TVRELVAIGR YPWHGALGRF GAADREKVEE AISLVGLKPL AHRLVDSLSG
160 170 180 190 200
GERQRAWIAM LVAQDSRCLL LDEPTSALDI AHQVDVLSLV HRLSQERGLT
210 220 230 240 250
VIAVLHDINM AARYCDYLVA LRGGEMIAQG TPAEIMRGET LEMIYGIPMG
260
ILPHPAGAAP VSFVY
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 31 | S → C in CAA29254 (PubMed:2823072).Curated | 1 | |
Sequence conflicti | 226 – 249 | MIAQG…YGIPM → SDCSGNACGNYARRNPRNDL WHPD in AAB61769 (PubMed:3301821).CuratedAdd BLAST | 24 | |
Sequence conflicti | 226 – 249 | MIAQG…YGIPM → SDCSGNACGNYARRNPRNDL WHPD no nucleotide entry (PubMed:8202364).CuratedAdd BLAST | 24 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M12486 Genomic DNA Translation: AAB61769.1 X05810 Genomic DNA Translation: CAA29254.1 U70214 Genomic DNA Translation: AAB08581.1 U00096 Genomic DNA Translation: AAC73262.1 AP009048 Genomic DNA Translation: BAB96727.1 |
PIRi | G64738, QRECFH |
RefSeqi | NP_414693.1, NC_000913.3 WP_001158931.1, NZ_STEB01000032.1 |
Genome annotation databases
EnsemblBacteriai | AAC73262; AAC73262; b0151 BAB96727; BAB96727; BAB96727 |
GeneIDi | 945250 |
KEGGi | ecj:JW0147 eco:b0151 |
PATRICi | fig|1411691.4.peg.2129 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M12486 Genomic DNA Translation: AAB61769.1 X05810 Genomic DNA Translation: CAA29254.1 U70214 Genomic DNA Translation: AAB08581.1 U00096 Genomic DNA Translation: AAC73262.1 AP009048 Genomic DNA Translation: BAB96727.1 |
PIRi | G64738, QRECFH |
RefSeqi | NP_414693.1, NC_000913.3 WP_001158931.1, NZ_STEB01000032.1 |
3D structure databases
SMRi | P07821 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4261072, 353 interactors 849632, 2 interactors |
ComplexPortali | CPX-4286, Ferric-hydroxamate ABC transporter complex |
IntActi | P07821, 5 interactors |
STRINGi | 511145.b0151 |
Protein family/group databases
TCDBi | 3.A.1.14.3, the atp-binding cassette (abc) superfamily |
Proteomic databases
PaxDbi | P07821 |
PRIDEi | P07821 |
Genome annotation databases
EnsemblBacteriai | AAC73262; AAC73262; b0151 BAB96727; BAB96727; BAB96727 |
GeneIDi | 945250 |
KEGGi | ecj:JW0147 eco:b0151 |
PATRICi | fig|1411691.4.peg.2129 |
Organism-specific databases
EchoBASEi | EB0300 |
Phylogenomic databases
eggNOGi | COG1120, Bacteria |
InParanoidi | P07821 |
PhylomeDBi | P07821 |
Enzyme and pathway databases
BioCyci | EcoCyc:FHUC-MONOMER MetaCyc:FHUC-MONOMER |
Miscellaneous databases
PROi | PR:P07821 |
Family and domain databases
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR003439, ABC_transporter-like IPR017871, ABC_transporter_CS IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF00005, ABC_tran, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00211, ABC_TRANSPORTER_1, 1 hit PS50893, ABC_TRANSPORTER_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | FHUC_ECOLI | |
Accessioni | P07821Primary (citable) accession number: P07821 Secondary accession number(s): P77548 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1988 |
Last sequence update: | November 1, 1997 | |
Last modified: | April 7, 2021 | |
This is version 174 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families