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Entry version 134 (11 Dec 2019)
Sequence version 1 (01 Aug 1988)
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Protein

Pullulanase

Gene

pulA

Organism
Klebsiella aerogenes (Enterobacter aerogenes)
Status
Reviewed-Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

  • Hydrolysis of (1->6)-alpha-D-glucosidic linkages in pullulan, amylopectin and glycogen, and in the alpha- and beta-limit dextrins of amylopectin and glycogen. EC:3.2.1.41

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei694NucleophileBy similarity1
Active sitei723Proton donorBy similarity1
<p>This subsection describes interesting single amino acid sites on the sequence that are not defined in any other subsection. This subsection can be displayed in different sections ('Function', 'PTM / Processing', 'Pathology and Biotech') according to its content.<p><a href='/help/site' target='_top'>More...</a></p>Sitei851Transition state stabilizerBy similarity1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionGlycosidase, Hydrolase

Enzyme and pathway databases

SABIO-RK: Biochemical Reaction Kinetics Database

More...
SABIO-RKi
P07811

Protein family/group databases

Carbohydrate-Active enZymes

More...
CAZyi
CBM41, Carbohydrate-Binding Module Family 41
CBM48, Carbohydrate-Binding Module Family 48
GH13, Glycoside Hydrolase Family 13

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Pullulanase (EC:3.2.1.41)
Alternative name(s):
Alpha-dextrin endo-1,6-alpha-glucosidase
Pullulan 6-glucanohydrolase
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:pulA
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiKlebsiella aerogenes (Enterobacter aerogenes)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri548 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeKlebsiella

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section denotes the presence of an N-terminal signal peptide.<p><a href='/help/signal' target='_top'>More...</a></p>Signal peptidei1 – 19Add BLAST19
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_000000142620 – 1096PullulanaseAdd BLAST1077

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM / Processing</a> section specifies the position(s) and the type of covalently attached lipid group(s).<p><a href='/help/lipid' target='_top'>More...</a></p>Lipidationi20N-palmitoyl cysteine2 Publications1
Lipidationi20S-diacylglycerol cysteine2 Publications1

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

PRoteomics IDEntifications database

More...
PRIDEi
P07811

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Homotrimer.

1 Publication

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

11096
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P07811

Database of comparative protein structure models

More...
ModBasei
Search...

Protein Data Bank in Europe - Knowledge Base

More...
PDBe-KBi
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

KEGG Orthology (KO)

More...
KOi
K01200

Family and domain databases

Conserved Domains Database

More...
CDDi
cd10315, CBM41_pullulanase, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
2.60.40.10, 1 hit
2.60.40.1180, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR013784, Carb-bd-like_fold
IPR005323, CBM41_pullulanase
IPR006047, Glyco_hydro_13_cat_dom
IPR004193, Glyco_hydro_13_N
IPR013780, Glyco_hydro_b
IPR017853, Glycoside_hydrolase_SF
IPR013783, Ig-like_fold
IPR014756, Ig_E-set
IPR011839, Pullul_strch
IPR024561, Pullul_strch_C
IPR041111, Pullulanase_Ins
IPR040671, Pullulanase_N2

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF02922, CBM_48, 1 hit
PF11852, DUF3372, 1 hit
PF03714, PUD, 1 hit
PF18494, Pullulanase_Ins, 1 hit
PF17967, Pullulanase_N2, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00642, Aamy, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF49452, SSF49452, 1 hit
SSF51445, SSF51445, 1 hit
SSF81296, SSF81296, 2 hits

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR02103, pullul_strch, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS51257, PROKAR_LIPOPROTEIN, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

P07811-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLRYTCHALF LGSLVLLSGC DNSSSSSTSG SPGSPGNPGN PGTPGTPDPQ
60 70 80 90 100
DVVVRLPDVA VPGEAVQASA RQAVIHLVDI AGITSSTPAD YATKNLYLWN
110 120 130 140 150
NETCDALSAP VADWNDVSTT PTGSDKYGPY WVIPLTKESG SINVIVRDGT
160 170 180 190 200
NKLIDSGRVS FSDFTDRTVS VIAGNSAVYD SRADAFRAAF GVALADAHWV
210 220 230 240 250
DKTTLLWPGG ENKPIVRLYY SHSSKVAADS NGEFSDKYVK LTPTTVNQQV
260 270 280 290 300
SMRFPHLASY PAFKLPDDVN VDELLQGDDG GIAESDGILS LSHPGADRRR
310 320 330 340 350
AGRYLCRRAE ALSYGAQLTD SGVTFRVWAP TAQQVELVIY SADKKVIASH
360 370 380 390 400
PMTRDSASGA WSWQGGSDLK GAFYRYAMTV YHPQSRKVEQ YEVTDPYAHS
410 420 430 440 450
LSTNSEYSQV VDLNDSALKP EGWDGLTMPH AQKTKADLAK MTIHESHIRD
460 470 480 490 500
LSAWDQTVPA ELRGKYLALT AQESNMVQHL KQLSASGVTH IELLPVFDLA
510 520 530 540 550
TVNEFSDKVA DIQQPFSRLC EVNSAVKSSE FAGYCDSGST VEEVLTQLKQ
560 570 580 590 600
NDSKDNPQVQ ALNTLVAQTD SYNWGYDPFH YTVPEGSYAT DPEGTARIKE
610 620 630 640 650
FRTMIQAIKQ DLGMNVIMDV VYNHTNAAGP TDRTSVLDKI VPWYYQRLNE
660 670 680 690 700
TTGSVESATC CSDSAPEHRM FAKLIADSLA VWTTDYKIDG FRFDLMGYHP
710 720 730 740 750
KAQILSAWER IKALNPDIYF FGEGWDSNQS DRFEIASQIN LKGTGIGTFS
760 770 780 790 800
DRLRDAVRGG GPFDSGDALR QNQGVGSGAG VLPNELTTLS DDQARHLADL
810 820 830 840 850
TRLGMAGNLA DFVLIDKDGA VKRGSEIDYN GAPGGYAADP TEVVNYVSKH
860 870 880 890 900
DNQTLWDMIS YKAAQEADLD TRVRMQAVSL ATVMLGQGIA FDQQGSELLR
910 920 930 940 950
SKSFTRDSYD SGDWFNRVDY SLQDNNYNVG MPRSSDDGSN YDIIARVKDA
960 970 980 990 1000
VATPGETELK QMTAFYQELT ALRKSSPLFT LGDGATVMKR VDFRNTGADQ
1010 1020 1030 1040 1050
QTGLLVMTID DGMQAGRQSG QPCRRHRGGD QRRAGKPDAA GLRRHIAPAE
1060 1070 1080 1090
RYSAGGGRPV AGERVQVAAD GSVTLPAWSV AVLELPQASR RALACR
Length:1,096
Mass (Da):119,336
Last modified:August 1, 1988 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iFE7D9167CDACFD79
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
M16187 Genomic DNA Translation: AAA25124.1 Sequence problems.

Protein sequence database of the Protein Information Resource

More...
PIRi
A26879

Genome annotation databases

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
ag:AAA25124

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16187 Genomic DNA Translation: AAA25124.1 Sequence problems.
PIRiA26879

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...
PDBei

Protein Data Bank RCSB

More...
RCSB PDBi

Protein Data Bank Japan

More...
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2FGZX-ray1.75A20-1090[»]
2FH6X-ray1.80A20-1090[»]
2FH8X-ray1.90A20-1087[»]
2FHBX-ray1.80A20-1090[»]
2FHCX-ray1.85A20-1090[»]
2FHFX-ray1.65A20-1090[»]
SMRiP07811
ModBaseiSearch...
PDBe-KBiSearch...

Protein family/group databases

CAZyiCBM41, Carbohydrate-Binding Module Family 41
CBM48, Carbohydrate-Binding Module Family 48
GH13, Glycoside Hydrolase Family 13

Proteomic databases

PRIDEiP07811

Genome annotation databases

KEGGiag:AAA25124

Phylogenomic databases

KOiK01200

Enzyme and pathway databases

SABIO-RKiP07811

Family and domain databases

CDDicd10315, CBM41_pullulanase, 1 hit
Gene3Di2.60.40.10, 1 hit
2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR013784, Carb-bd-like_fold
IPR005323, CBM41_pullulanase
IPR006047, Glyco_hydro_13_cat_dom
IPR004193, Glyco_hydro_13_N
IPR013780, Glyco_hydro_b
IPR017853, Glycoside_hydrolase_SF
IPR013783, Ig-like_fold
IPR014756, Ig_E-set
IPR011839, Pullul_strch
IPR024561, Pullul_strch_C
IPR041111, Pullulanase_Ins
IPR040671, Pullulanase_N2
PfamiView protein in Pfam
PF02922, CBM_48, 1 hit
PF11852, DUF3372, 1 hit
PF03714, PUD, 1 hit
PF18494, Pullulanase_Ins, 1 hit
PF17967, Pullulanase_N2, 1 hit
SMARTiView protein in SMART
SM00642, Aamy, 1 hit
SUPFAMiSSF49452, SSF49452, 1 hit
SSF51445, SSF51445, 1 hit
SSF81296, SSF81296, 2 hits
TIGRFAMsiTIGR02103, pullul_strch, 1 hit
PROSITEiView protein in PROSITE
PS51257, PROKAR_LIPOPROTEIN, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiPULA_KLEAE
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P07811
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: December 11, 2019
This is version 134 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
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