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Protein

Valine--tRNA ligase, mitochondrial

Gene

VAS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Present with 358 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei706ATPBy similarity1

GO - Molecular functioni

  • aminoacyl-tRNA editing activity Source: InterPro
  • ATP binding Source: UniProtKB-KW
  • valine-tRNA ligase activity Source: SGD

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30804-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Valine--tRNA ligase, mitochondrial (EC:6.1.1.9)
Alternative name(s):
Valyl-tRNA synthetase
Short name:
ValRS
Gene namesi
Name:VAS1
Ordered Locus Names:YGR094W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR094W
SGDiS000003326 VAS1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 47MitochondrionAdd BLAST47
ChainiPRO_000003583948 – 1104Valine--tRNA ligase, mitochondrialAdd BLAST1057

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei73PhosphoserineCombined sources1
Modified residuei294PhosphoserineCombined sources1
Modified residuei332PhosphoserineCombined sources1
Modified residuei707PhosphoserineCombined sources1
Modified residuei1003PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP07806
PRIDEiP07806

PTM databases

iPTMnetiP07806

Interactioni

Protein-protein interaction databases

BioGridi33337, 81 interactors
DIPiDIP-6328N
IntActiP07806, 18 interactors
MINTiP07806
STRINGi4932.YGR094W

Structurei

3D structure databases

ProteinModelPortaliP07806
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi190 – 200"HIGH" regionAdd BLAST11
Motifi703 – 707"KMSKS" region5

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00550000074727
HOGENOMiHOG000020094
InParanoidiP07806
KOiK01873
OMAiFATKLWN
OrthoDBiEOG092C0H3K

Family and domain databases

CDDicd07962 Anticodon_Ia_Val, 1 hit
Gene3Di1.10.287.380, 1 hit
3.40.50.620, 2 hits
3.90.740.10, 2 hits
HAMAPiMF_02004 Val_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033705 Anticodon_Ia_Val
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR010978 tRNA-bd_arm
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR037118 Val-tRNA_synth_C_sf
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR002303 Valyl-tRNA_ligase
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00986 TRNASYNTHVAL
SUPFAMiSSF46589 SSF46589, 1 hit
SSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00422 valS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket
Isoform Mitochondrial (identifier: P07806-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MNKWLNTLSK TFTFRLLNCH YRRSLPLCQN FSLKKSLTHN QVRFFKMSDL
60 70 80 90 100
DNLPPVDPKT GEVIINPLKE DGSPKTPKEI EKEKKKAEKL LKFAAKQAKK
110 120 130 140 150
NAAATTGASQ KKPKKKKEVE PIPEFIDKTV PGEKKILVSL DDPALKAYNP
160 170 180 190 200
ANVESSWYDW WIKTGVFEPE FTADGKVKPE GVFCIPAPPP NVTGALHIGH
210 220 230 240 250
ALTIAIQDSL IRYNRMKGKT VLFLPGFDHA GIATQSVVEK QIWAKDRKTR
260 270 280 290 300
HDYGREAFVG KVWEWKEEYH SRIKNQIQKL GASYDWSREA FTLSPELTKS
310 320 330 340 350
VEEAFVRLHD EGVIYRASRL VNWSVKLNTA ISNLEVENKD VKSRTLLSVP
360 370 380 390 400
GYDEKVEFGV LTSFAYPVIG SDEKLIIATT RPETIFGDTA VAVHPDDDRY
410 420 430 440 450
KHLHGKFIQH PFLPRKIPII TDKEAVDMEF GTGAVKITPA HDQNDYNTGK
460 470 480 490 500
RHNLEFINIL TDDGLLNEEC GPEWQGMKRF DARKKVIEQL KEKNLYVGQE
510 520 530 540 550
DNEMTIPTCS RSGDIIEPLL KPQWWVSQSE MAKDAIKVVR DGQITITPKS
560 570 580 590 600
SEAEYFHWLG NIQDWCISRQ LWWGHRCPVY FINIEGEEHD RIDGDYWVAG
610 620 630 640 650
RSMEEAEKKA AAKYPNSKFT LEQDEDVLDT WFSSGLWPFS TLGWPEKTKD
660 670 680 690 700
METFYPFSML ETGWDILFFW VTRMILLGLK LTGSVPFKEV FCHSLVRDAQ
710 720 730 740 750
GRKMSKSLGN VIDPLDVITG IKLDDLHAKL LQGNLDPREV EKAKIGQKES
760 770 780 790 800
YPNGIPQCGT DAMRFALCAY TTGGRDINLD ILRVEGYRKF CNKIYQATKF
810 820 830 840 850
ALMRLGDDYQ PPATEGLSGN ESLVEKWILH KLTETSKIVN EALDKRDFLT
860 870 880 890 900
STSSIYEFWY LICDVYIENS KYLIQEGSAI EKKSAKDTLY ILLDNALKLI
910 920 930 940 950
HPFMPFISEE MWQRLPKRST EKAASIVKAS YPVYVSEYDD VKSANAYDLV
960 970 980 990 1000
LNITKEARSL LSEYNILKNG KVFVESNHEE YFKTAEDQKD SIVSLIKAID
1010 1020 1030 1040 1050
EVTVVRDASE IPEGCVLQSV NPEVNVHLLV KGHVDIDAEI AKVQKKLEKA
1060 1070 1080 1090 1100
KKSKNGIEQT INSKDYETKA NTQAKEANKS KLDNTVAEIE GLEATIENLK

RLKL
Length:1,104
Mass (Da):125,770
Last modified:October 1, 1996 - v2
Checksum:i6493AEF37ECD4A7C
GO
Isoform Cytoplasmic (identifier: P07806-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-46: Missing.

Note: Produced by alternative initiation at Met-47 of isoform Mitochondrial.
Show »
Length:1,058
Mass (Da):120,126
Checksum:i123D6E8A5494FB73
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti147A → G in AAA35207 (PubMed:3294828).Curated1
Sequence conflicti540R → K in AAA35207 (PubMed:3294828).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0189101 – 46Missing in isoform Cytoplasmic. CuratedAdd BLAST46

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02719 Genomic DNA Translation: AAA35207.1
Z72879 Genomic DNA Translation: CAA97097.1
M18392 Genomic DNA Translation: AAA35205.1
M18392 Genomic DNA Translation: AAA35206.1
BK006941 Genomic DNA Translation: DAA08187.1
PIRiS64389 SYBYVT
RefSeqiNP_011608.1, NM_001181223.1 [P07806-1]

Genome annotation databases

EnsemblFungiiYGR094W; YGR094W; YGR094W [P07806-1]
GeneIDi852986
KEGGisce:YGR094W

Keywords - Coding sequence diversityi

Alternative initiation

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02719 Genomic DNA Translation: AAA35207.1
Z72879 Genomic DNA Translation: CAA97097.1
M18392 Genomic DNA Translation: AAA35205.1
M18392 Genomic DNA Translation: AAA35206.1
BK006941 Genomic DNA Translation: DAA08187.1
PIRiS64389 SYBYVT
RefSeqiNP_011608.1, NM_001181223.1 [P07806-1]

3D structure databases

ProteinModelPortaliP07806
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33337, 81 interactors
DIPiDIP-6328N
IntActiP07806, 18 interactors
MINTiP07806
STRINGi4932.YGR094W

PTM databases

iPTMnetiP07806

Proteomic databases

PaxDbiP07806
PRIDEiP07806

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR094W; YGR094W; YGR094W [P07806-1]
GeneIDi852986
KEGGisce:YGR094W

Organism-specific databases

EuPathDBiFungiDB:YGR094W
SGDiS000003326 VAS1

Phylogenomic databases

GeneTreeiENSGT00550000074727
HOGENOMiHOG000020094
InParanoidiP07806
KOiK01873
OMAiFATKLWN
OrthoDBiEOG092C0H3K

Enzyme and pathway databases

BioCyciYEAST:G3O-30804-MONOMER

Miscellaneous databases

PROiPR:P07806

Family and domain databases

CDDicd07962 Anticodon_Ia_Val, 1 hit
Gene3Di1.10.287.380, 1 hit
3.40.50.620, 2 hits
3.90.740.10, 2 hits
HAMAPiMF_02004 Val_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033705 Anticodon_Ia_Val
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR010978 tRNA-bd_arm
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR037118 Val-tRNA_synth_C_sf
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR002303 Valyl-tRNA_ligase
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00986 TRNASYNTHVAL
SUPFAMiSSF46589 SSF46589, 1 hit
SSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00422 valS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSYV_YEAST
AccessioniPrimary (citable) accession number: P07806
Secondary accession number(s): D6VUM6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: October 1, 1996
Last modified: September 12, 2018
This is version 189 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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