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Protein

Fructose-bisphosphate aldolase

Gene

Ald

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May take part in developmental stage-specific or tissue -specific sugar-phosphate metabolisms. Protein acts on two substrates fructose 1,6-bisphosphate and fructose 1-phosphate (like other class I aldolases).1 Publication

Catalytic activityi

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.1 Publication

pH dependencei

Optimum pH is 7.2-7.8 for isozyme alpha, and 7.2-7.5 for isozymes beta and gamma.1 Publication

Temperature dependencei

Thermostability of the isozymes, calculated as the temperature causing half maximal stability, is 42-43 degrees Celsius for isozymes alpha and beta and 45 degrees Celsius for isozyme gamma.1 Publication

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (Pgi)
  3. ATP-dependent 6-phosphofructokinase (Pfk), ATP-dependent 6-phosphofructokinase (Pfk-RB), ATP-dependent 6-phosphofructokinase (Pfk), ATP-dependent 6-phosphofructokinase (Pfk-RC), ATP-dependent 6-phosphofructokinase (Pfk-RB), ATP-dependent 6-phosphofructokinase (Pfk)
  4. Fructose-bisphosphate aldolase (Ald), Fructose-bisphosphate aldolase (Ald), Fructose-bisphosphate aldolase (Ald-RI), Fructose-bisphosphate aldolase (Ald-RH), Fructose-bisphosphate aldolase (Ald), Fructose-bisphosphate aldolase (Ald)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei56Substrate1
Binding sitei147Substrate1
Active sitei188Proton acceptorBy similarity1
Active sitei230Schiff-base intermediate with dihydroxyacetone-P1
Sitei361Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphate1

GO - Molecular functioni

  • fructose-bisphosphate aldolase activity Source: FlyBase

GO - Biological processi

  • glucose homeostasis Source: FlyBase
  • glycolytic process Source: FlyBase
  • mesoderm development Source: FlyBase

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandSchiff base

Enzyme and pathway databases

ReactomeiR-DME-114608 Platelet degranulation
R-DME-6798695 Neutrophil degranulation
R-DME-70171 Glycolysis
R-DME-70263 Gluconeogenesis
R-DME-70350 Fructose catabolism
SABIO-RKiP07764
UniPathwayi
UPA00109;UER00183

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-bisphosphate aldolase (EC:4.1.2.13)
Gene namesi
Name:Ald
ORF Names:CG6058
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0000064 Ald

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00002169272 – 361Fructose-bisphosphate aldolaseAdd BLAST360

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylthreonine2 Publications1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP07764
PRIDEiP07764

PTM databases

iPTMnetiP07764

Expressioni

Tissue specificityi

Isozyme gamma is mainly expressed in the heads and partly in the thoraxes of adult flies. Isozyme alpha is expressed in all adult tissues. Isozyme beta is mainly expressed in adult abdominal regions and is also expressed in lesser amounts in other parts of the body and in earlier developmental stages. Isozyme alpha-beta shows strong expression in the abdomens of adults. Isozyme beta-gamma is expressed in adult heads.2 Publications

Developmental stagei

Isozyme gamma is first detected during late embryogenesis, is present through larval stages, declines in abundance during pupal stages and is maximum at eclosion. Isozyme beta is abundant during the earliest stages of embryogenesis, declines in amount and increases prior to hatching. Isozyme alpha is found only during the adult stage. Expression correlates to dietary behavior: high dietary activity in the larvae and adults causes active glycolysis and high expression levels. Isozyme alpha-beta is detected in the pupae and adults and isozyme beta-gamma in the adult.1 Publication

Gene expression databases

BgeeiFBgn0000064 Expressed in 33 organ(s), highest expression level in arthropod fat body
ExpressionAtlasiP07764 baseline and differential
GenevisibleiP07764 DM

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

BioGridi68080, 93 interactors
IntActiP07764, 67 interactors
STRINGi7227.FBpp0302781

Structurei

Secondary structure

1361
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP07764
SMRiP07764
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07764

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1557 Eukaryota
COG3588 LUCA
GeneTreeiENSGT00390000010235
InParanoidiP07764
KOiK01623
OMAiMTHQYPA
OrthoDBiEOG091G0A9T
PhylomeDBiP07764

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR029768 Aldolase_I_AS
IPR013785 Aldolase_TIM
IPR000741 FBA_I
PfamiView protein in Pfam
PF00274 Glycolytic, 1 hit
PROSITEiView protein in PROSITE
PS00158 ALDOLASE_CLASS_I, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket
Note: Two additional mRNAs also exist (Alpha-beta and Beta-gamma). The translation product of Alpha-beta mRNA is isoform Alpha.

This entry has 3 described isoforms and 3 potential isoforms that are computationally mapped.iShow all

Isoform Gamma (identifier: P07764-1) [UniParc]FASTAAdd to basket
Also known as: 4C, B, C, D

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MTTYFNYPSK ELQDELREIA QKIVAPGKGI LAADESGPTM GKRLQDIGVE
60 70 80 90 100
NTEDNRRAYR QLLFSTDPKL AENISGVILF HETLYQKADD GTPFAEILKK
110 120 130 140 150
KGIILGIKVD KGVVPLFGSE DEVTTQGLDD LAARCAQYKK DGCDFAKWRC
160 170 180 190 200
VLKIGKNTPS YQSILENANV LARYASICQS QRIVPIVEPE VLPDGDHDLD
210 220 230 240 250
RAQKVTETVL AAVYKALSDH HVYLEGTLLK PNMVTAGQSA KKNTPEEIAL
260 270 280 290 300
ATVQALRRTV PAAVTGVTFL SGGQSEEEAT VNLSAINNVP LIRPWALTFS
310 320 330 340 350
YGRALQASVL RAWAGKKENI AAGQNELLKR AKANGDAAQG KYVAGSAGAG
360
SGSLFVANHA Y
Length:361
Mass (Da):39,047
Last modified:January 23, 2007 - v5
Checksum:iD8DA46E273668CE9
GO
Isoform Alpha (identifier: P07764-2) [UniParc]FASTAAdd to basket
Also known as: 4A, E

The sequence of this isoform differs from the canonical sequence as follows:
     336-361: DAAQGKYVAGSAGAGSGSLFVANHAY → EAACGNYTAGSVKGFAGKDTLHVDDHRY

Show »
Length:363
Mass (Da):39,561
Checksum:iE48CC61438C85B64
GO
Isoform Beta (identifier: P07764-3) [UniParc]FASTAAdd to basket
Also known as: 4B, H

The sequence of this isoform differs from the canonical sequence as follows:
     335-361: GDAAQGKYVAGSAGAGSGSLFVANHAY → SQACQGIYVPGSIPSFAGNANLFVAQHKY

Show »
Length:363
Mass (Da):39,589
Checksum:i7B326172746BF31D
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
C8VV14C8VV14_DROME
Fructose-bisphosphate aldolase
Ald ALD, ald, Ald-RC, BcDNA:LP07735, dALDOA Ald ALD, ald, Ald-RC, BcDNA:LP07735, dALDOA, Dmel\CG6058, FBA, T12, T4, T6, T7, CG6058, Dmel_CG6058
361Annotation score:
A4V3G1A4V3G1_DROME
Fructose-bisphosphate aldolase
Ald ALD, ald, Ald-RE, BcDNA:LP07735, dALDOA Ald ALD, ald, Ald-RE, BcDNA:LP07735, dALDOA, Dmel\CG6058, FBA, T12, T4, T6, T7, CG6058, Dmel_CG6058
363Annotation score:
F3YDE2F3YDE2_DROME
Fructose-bisphosphate aldolase
Ald ALD, ald, Ald-RH, BcDNA:LP07735, dALDOA Ald ALD, ald, Ald-RH, BcDNA:LP07735, dALDOA, Dmel\CG6058, FBA, T12, T4, T6, T7, CG6058, Dmel_CG6058
363Annotation score:

Sequence cautioni

The sequence AAM75045 differs from that shown. Reason: Frameshift at positions 267 and 333.Curated
The sequence ABH06768 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence ABH06769 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence ABH06770 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence ABH06771 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence ABH06772 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence ABH06773 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence ABH06774 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence ABH06775 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence ABH06776 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence ABH06777 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence ABH06778 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence ABH06779 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAA42666 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti40M → H in BAA01236 (PubMed:1339430).Curated1
Sequence conflicti40M → H in BAA01237 (PubMed:1339430).Curated1
Sequence conflicti40M → H in BAA01238 (PubMed:1339430).Curated1
Sequence conflicti40M → H in BAA01592 (PubMed:7487099).Curated1
Sequence conflicti111K → E in CAA42666 (PubMed:1732743).Curated1
Sequence conflicti111K → E in CAA42667 (PubMed:1732743).Curated1
Sequence conflicti181Q → E in BAA01236 (PubMed:1339430).Curated1
Sequence conflicti181Q → E in BAA01237 (PubMed:1339430).Curated1
Sequence conflicti181Q → E in BAA01238 (PubMed:1339430).Curated1
Sequence conflicti181Q → E in BAA01592 (PubMed:7487099).Curated1
Sequence conflicti201R → G in CAA42666 (PubMed:1732743).Curated1
Sequence conflicti201R → G in CAA42667 (PubMed:1732743).Curated1
Sequence conflicti251A → T in CAA42666 (PubMed:1732743).Curated1
Sequence conflicti251A → T in CAA42667 (PubMed:1732743).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_000221335 – 361GDAAQ…ANHAY → SQACQGIYVPGSIPSFAGNA NLFVAQHKY in isoform Beta. 1 PublicationAdd BLAST27
Alternative sequenceiVSP_000223336 – 361DAAQG…ANHAY → EAACGNYTAGSVKGFAGKDT LHVDDHRY in isoform Alpha. 1 PublicationAdd BLAST26

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10446 Genomic DNA Translation: BAA01236.1
D10446 Genomic DNA Translation: BAA01237.1
D10446 Genomic DNA Translation: BAA01238.1
M98351 Genomic DNA Translation: AAA99426.1
M98351 Genomic DNA Translation: AAA99427.1
M98351 Genomic DNA Translation: AAA99428.1
X60064 Genomic DNA Translation: CAA42666.1 Sequence problems.
X60064 Genomic DNA Translation: CAA42667.1
X60064 Genomic DNA Translation: CAA42668.1
D10762 mRNA Translation: BAA01592.1
DQ864133 Genomic DNA Translation: ABH06768.1 Sequence problems.
DQ864134 Genomic DNA Translation: ABH06769.1 Sequence problems.
DQ864135 Genomic DNA Translation: ABH06770.1 Sequence problems.
DQ864136 Genomic DNA Translation: ABH06771.1 Sequence problems.
DQ864137 Genomic DNA Translation: ABH06772.1 Sequence problems.
DQ864138 Genomic DNA Translation: ABH06773.1 Sequence problems.
DQ864139 Genomic DNA Translation: ABH06774.1 Sequence problems.
DQ864140 Genomic DNA Translation: ABH06775.1 Sequence problems.
DQ864141 Genomic DNA Translation: ABH06776.1 Sequence problems.
DQ864142 Genomic DNA Translation: ABH06777.1 Sequence problems.
DQ864143 Genomic DNA Translation: ABH06778.1 Sequence problems.
DQ864144 Genomic DNA Translation: ABH06779.1 Sequence problems.
AE014297 Genomic DNA Translation: AAF56579.1
AE014297 Genomic DNA Translation: AAN14381.1
AE014297 Genomic DNA Translation: AAN14382.1
AE014297 Genomic DNA Translation: AAN14384.1
AE014297 Genomic DNA Translation: AAS65220.1
AY058667 mRNA Translation: AAL13896.1
AY128452 mRNA Translation: AAM75045.1 Frameshift.
PIRiA42027 ADFFR
A42263
B42027 ADFF
B42263
C42263
JX0233
S68360
RefSeqiNP_001262985.1, NM_001276056.1 [P07764-3]
NP_001287541.1, NM_001300612.1 [P07764-1]
NP_524515.2, NM_079791.5 [P07764-2]
NP_733140.2, NM_170261.3 [P07764-3]
NP_733141.1, NM_170262.2 [P07764-1]
NP_733142.1, NM_170263.4 [P07764-1]
NP_733143.1, NM_170264.3 [P07764-1]
NP_733144.3, NM_170265.4 [P07764-1]
NP_733145.3, NM_170266.4 [P07764-2]
NP_996300.1, NM_206577.3 [P07764-3]
UniGeneiDm.2349

Genome annotation databases

EnsemblMetazoaiFBtr0084994; FBpp0084367; FBgn0000064 [P07764-1]
FBtr0084995; FBpp0084368; FBgn0000064 [P07764-1]
FBtr0084999; FBpp0084372; FBgn0000064 [P07764-1]
FBtr0085000; FBpp0084373; FBgn0000064 [P07764-2]
FBtr0085001; FBpp0089214; FBgn0000064 [P07764-3]
FBtr0114544; FBpp0113036; FBgn0000064 [P07764-2]
FBtr0306657; FBpp0297612; FBgn0000064 [P07764-3]
FBtr0310660; FBpp0302780; FBgn0000064 [P07764-1]
FBtr0310661; FBpp0302781; FBgn0000064 [P07764-3]
FBtr0339614; FBpp0308680; FBgn0000064 [P07764-1]
GeneIDi43183
KEGGidme:Dmel_CG6058

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiALF_DROME
AccessioniPrimary (citable) accession number: P07764
Secondary accession number(s): A4V3F9
, A5XCY2, P29841, Q24236, Q24237, Q7KRY9, Q8IMS1, Q8MQQ4, Q9VBG2, Q9VBG3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: January 23, 2007
Last modified: September 12, 2018
This is version 188 of the entry and version 5 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health

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