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Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.By similarity

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.

Pathwayi: glycogen biosynthesis

This protein is involved in the pathway glycogen biosynthesis, which is part of Glycan biosynthesis.
View all proteins of this organism that are known to be involved in the pathway glycogen biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei405NucleophileBy similarity1
Active sitei458Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoliWiki
  • glycogen biosynthetic process Source: EcoCyc

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processCarbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

BioCyciEcoCyc:GLYCOGEN-BRANCH-MONOMER
MetaCyc:GLYCOGEN-BRANCH-MONOMER
UniPathwayiUPA00164

Protein family/group databases

CAZyiCBM48 Carbohydrate-Binding Module Family 48
GH13 Glycoside Hydrolase Family 13

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgB (EC:2.4.1.18)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name:
BE
Gene namesi
Name:glgB
Ordered Locus Names:b3432, JW3395
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10378 glgB

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001887021 – 7281,4-alpha-glucan branching enzyme GlgBAdd BLAST728

Proteomic databases

EPDiP07762
PaxDbiP07762
PRIDEiP07762

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi4262503, 314 interactors
IntActiP07762, 13 interactors
STRINGi316385.ECDH10B_3606

Structurei

Secondary structure

1728
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi121 – 123Combined sources3
Beta strandi125 – 134Combined sources10
Beta strandi136 – 143Combined sources8
Beta strandi145 – 148Combined sources4
Beta strandi150 – 154Combined sources5
Helixi155 – 157Combined sources3
Turni161 – 163Combined sources3
Turni170 – 172Combined sources3
Beta strandi174 – 180Combined sources7
Beta strandi187 – 193Combined sources7
Beta strandi199 – 202Combined sources4
Beta strandi208 – 211Combined sources4
Turni213 – 215Combined sources3
Beta strandi217 – 219Combined sources3
Helixi231 – 237Combined sources7
Beta strandi244 – 248Combined sources5
Turni250 – 252Combined sources3
Turni257 – 259Combined sources3
Helixi265 – 278Combined sources14
Beta strandi282 – 287Combined sources6
Helixi295 – 297Combined sources3
Beta strandi303 – 308Combined sources6
Helixi310 – 312Combined sources3
Helixi315 – 327Combined sources13
Beta strandi331 – 336Combined sources6
Beta strandi338 – 340Combined sources3
Helixi351 – 353Combined sources3
Beta strandi356 – 358Combined sources3
Helixi380 – 396Combined sources17
Beta strandi401 – 404Combined sources4
Helixi408 – 411Combined sources4
Helixi433 – 448Combined sources16
Beta strandi454 – 457Combined sources4
Turni464 – 467Combined sources4
Turni470 – 473Combined sources4
Beta strandi478 – 481Combined sources4
Helixi483 – 494Combined sources12
Helixi497 – 502Combined sources6
Helixi505 – 508Combined sources4
Helixi509 – 511Combined sources3
Turni512 – 516Combined sources5
Beta strandi519 – 523Combined sources5
Helixi525 – 527Combined sources3
Helixi535 – 538Combined sources4
Helixi543 – 559Combined sources17
Beta strandi560 – 567Combined sources8
Turni568 – 573Combined sources6
Helixi586 – 589Combined sources4
Beta strandi591 – 593Combined sources3
Helixi595 – 610Combined sources16
Helixi614 – 616Combined sources3
Turni617 – 621Combined sources5
Helixi623 – 625Combined sources3
Beta strandi626 – 633Combined sources8
Turni634 – 637Combined sources4
Beta strandi638 – 644Combined sources7
Beta strandi650 – 656Combined sources7
Beta strandi658 – 660Combined sources3
Beta strandi662 – 665Combined sources4
Beta strandi673 – 680Combined sources8
Helixi685 – 687Combined sources3
Beta strandi713 – 715Combined sources3
Beta strandi719 – 726Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GTYmodel-A24-103[»]
1M7XX-ray2.30A/B/C/D113-728[»]
4LPCX-ray2.39A/B/C/D117-728[»]
4LQ1X-ray2.55A/B/C/D117-728[»]
5E6YX-ray2.60A/B/C/D117-728[»]
5E6ZX-ray1.88A/B/C/D117-728[»]
5E70X-ray2.33A/B/C/D117-728[»]
ProteinModelPortaliP07762
SMRiP07762
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07762

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105C9C Bacteria
COG0296 LUCA
HOGENOMiHOG000283037
InParanoidiP07762
KOiK00700
OMAiEVVHGKS
PhylomeDBiP07762

Family and domain databases

Gene3Di2.60.40.10, 1 hit
2.60.40.1180, 1 hit
HAMAPiMF_00685 GlgB, 1 hit
InterProiView protein in InterPro
IPR006048 A-amylase/branching_C
IPR037439 Branching_enzy
IPR006407 GlgB
IPR006047 Glyco_hydro_13_cat_dom
IPR004193 Glyco_hydro_13_N
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
IPR013783 Ig-like_fold
IPR014756 Ig_E-set
PANTHERiPTHR43651 PTHR43651, 1 hit
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF02806 Alpha-amylase_C, 1 hit
PF02922 CBM_48, 1 hit
PIRSFiPIRSF000463 GlgB, 1 hit
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit
SSF81296 SSF81296, 2 hits
TIGRFAMsiTIGR01515 branching_enzym, 1 hit

Sequencei

Sequence statusi: Complete.

P07762-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDRIDRDVI NALIAGHFAD PFSVLGMHKT TAGLEVRALL PDATDVWVIE
60 70 80 90 100
PKTGRKLAKL ECLDSRGFFS GVIPRRKNFF RYQLAVVWHG QQNLIDDPYR
110 120 130 140 150
FGPLIQEMDA WLLSEGTHLR PYETLGAHAD TMDGVTGTRF SVWAPNARRV
160 170 180 190 200
SVVGQFNYWD GRRHPMRLRK ESGIWELFIP GAHNGQLYKY EMIDANGNLR
210 220 230 240 250
LKSDPYAFEA QMRPETASLI CGLPEKVVQT EERKKANQFD APISIYEVHL
260 270 280 290 300
GSWRRHTDNN FWLSYRELAD QLVPYAKWMG FTHLELLPIN EHPFDGSWGY
310 320 330 340 350
QPTGLYAPTR RFGTRDDFRY FIDAAHAAGL NVILDWVPGH FPTDDFALAE
360 370 380 390 400
FDGTNLYEHS DPREGYHQDW NTLIYNYGRR EVSNFLVGNA LYWIERFGID
410 420 430 440 450
ALRVDAVASM IYRDYSRKEG EWIPNEFGGR ENLEAIEFLR NTNRILGEQV
460 470 480 490 500
SGAVTMAEES TDFPGVSRPQ DMGGLGFWYK WNLGWMHDTL DYMKLDPVYR
510 520 530 540 550
QYHHDKLTFG ILYNYTENFV LPLSHDEVVH GKKSILDRMP GDAWQKFANL
560 570 580 590 600
RAYYGWMWAF PGKKLLFMGN EFAQGREWNH DASLDWHLLE GGDNWHHGVQ
610 620 630 640 650
RLVRDLNLTY RHHKAMHELD FDPYGFEWLV VDDKERSVLI FVRRDKEGNE
660 670 680 690 700
IIVASNFTPV PRHDYRFGIN QPGKWREILN TDSMHYHGSN AGNGGTVHSD
710 720
EIASHGRQHS LSLTLPPLAT IWLVREAE
Length:728
Mass (Da):84,337
Last modified:August 1, 1988 - v1
Checksum:i0F20AF3677BF2015
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13751 Genomic DNA Translation: AAA23872.1
U18997 Genomic DNA Translation: AAA58230.1
U00096 Genomic DNA Translation: AAC76457.1
AP009048 Genomic DNA Translation: BAE77860.1
PIRiA25498 NQECA
RefSeqiNP_417890.1, NC_000913.3
WP_001283723.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76457; AAC76457; b3432
BAE77860; BAE77860; BAE77860
GeneIDi947940
KEGGiecj:JW3395
eco:b3432
PATRICifig|1411691.4.peg.3296

Similar proteinsi

Entry informationi

Entry nameiGLGB_ECOLI
AccessioniPrimary (citable) accession number: P07762
Secondary accession number(s): Q2M796
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: March 28, 2018
This is version 161 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

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