UniProtKB - P07762 (GLGB_ECOLI)
Protein
1,4-alpha-glucan branching enzyme GlgB
Gene
glgB
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.By similarity
Catalytic activityi
- Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. EC:2.4.1.18
: glycogen biosynthesis Pathwayi
This protein is involved in the pathway glycogen biosynthesis, which is part of Glycan biosynthesis.View all proteins of this organism that are known to be involved in the pathway glycogen biosynthesis and in Glycan biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 405 | NucleophileBy similarity | 1 | |
Active sitei | 458 | Proton donorBy similarity | 1 |
GO - Molecular functioni
- 1,4-alpha-glucan branching enzyme activity Source: EcoCyc
- 1,4-alpha-glucan branching enzyme activity (using a glucosylated glycogenin as primer for glycogen synthesis) Source: UniProtKB-EC
- cation binding Source: InterPro
- hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro
GO - Biological processi
- carbohydrate metabolic process Source: GO_Central
- cellular response to DNA damage stimulus Source: EcoliWiki
- glycogen biosynthetic process Source: EcoCyc
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Biological process | Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism |
Enzyme and pathway databases
BioCyci | EcoCyc:GLYCOGEN-BRANCH-MONOMER MetaCyc:GLYCOGEN-BRANCH-MONOMER |
UniPathwayi | UPA00164 |
Protein family/group databases
CAZyi | CBM48, Carbohydrate-Binding Module Family 48 GH13, Glycoside Hydrolase Family 13 |
Names & Taxonomyi
Protein namesi | Recommended name: 1,4-alpha-glucan branching enzyme GlgB (EC:2.4.1.18)Alternative name(s): 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase Alpha-(1->4)-glucan branching enzyme Glycogen branching enzyme Short name: BE |
Gene namesi | Name:glgB Ordered Locus Names:b3432, JW3395 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000188702 | 1 – 728 | 1,4-alpha-glucan branching enzyme GlgBAdd BLAST | 728 |
Proteomic databases
jPOSTi | P07762 |
PaxDbi | P07762 |
PRIDEi | P07762 |
Interactioni
Subunit structurei
Monomer.
Protein-protein interaction databases
BioGRIDi | 4262503, 314 interactors |
IntActi | P07762, 13 interactors |
STRINGi | 511145.b3432 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P07762 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P07762 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0296, Bacteria |
HOGENOMi | CLU_004245_3_2_6 |
InParanoidi | P07762 |
PhylomeDBi | P07762 |
Family and domain databases
CDDi | cd02855, E_set_GBE_prok_N, 1 hit |
Gene3Di | 2.60.40.10, 1 hit 2.60.40.1180, 1 hit |
HAMAPi | MF_00685, GlgB, 1 hit |
InterProi | View protein in InterPro IPR006048, A-amylase/branching_C IPR037439, Branching_enzy IPR006407, GlgB IPR044143, GlgB_N_E_set_prok IPR006047, Glyco_hydro_13_cat_dom IPR004193, Glyco_hydro_13_N IPR013780, Glyco_hydro_b IPR017853, Glycoside_hydrolase_SF IPR013783, Ig-like_fold IPR014756, Ig_E-set |
PANTHERi | PTHR43651, PTHR43651, 1 hit |
Pfami | View protein in Pfam PF00128, Alpha-amylase, 1 hit PF02806, Alpha-amylase_C, 1 hit PF02922, CBM_48, 1 hit |
PIRSFi | PIRSF000463, GlgB, 1 hit |
SMARTi | View protein in SMART SM00642, Aamy, 1 hit |
SUPFAMi | SSF51445, SSF51445, 1 hit SSF81296, SSF81296, 2 hits |
TIGRFAMsi | TIGR01515, branching_enzym, 1 hit |
i Sequence
Sequence statusi: Complete.
P07762-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSDRIDRDVI NALIAGHFAD PFSVLGMHKT TAGLEVRALL PDATDVWVIE
60 70 80 90 100
PKTGRKLAKL ECLDSRGFFS GVIPRRKNFF RYQLAVVWHG QQNLIDDPYR
110 120 130 140 150
FGPLIQEMDA WLLSEGTHLR PYETLGAHAD TMDGVTGTRF SVWAPNARRV
160 170 180 190 200
SVVGQFNYWD GRRHPMRLRK ESGIWELFIP GAHNGQLYKY EMIDANGNLR
210 220 230 240 250
LKSDPYAFEA QMRPETASLI CGLPEKVVQT EERKKANQFD APISIYEVHL
260 270 280 290 300
GSWRRHTDNN FWLSYRELAD QLVPYAKWMG FTHLELLPIN EHPFDGSWGY
310 320 330 340 350
QPTGLYAPTR RFGTRDDFRY FIDAAHAAGL NVILDWVPGH FPTDDFALAE
360 370 380 390 400
FDGTNLYEHS DPREGYHQDW NTLIYNYGRR EVSNFLVGNA LYWIERFGID
410 420 430 440 450
ALRVDAVASM IYRDYSRKEG EWIPNEFGGR ENLEAIEFLR NTNRILGEQV
460 470 480 490 500
SGAVTMAEES TDFPGVSRPQ DMGGLGFWYK WNLGWMHDTL DYMKLDPVYR
510 520 530 540 550
QYHHDKLTFG ILYNYTENFV LPLSHDEVVH GKKSILDRMP GDAWQKFANL
560 570 580 590 600
RAYYGWMWAF PGKKLLFMGN EFAQGREWNH DASLDWHLLE GGDNWHHGVQ
610 620 630 640 650
RLVRDLNLTY RHHKAMHELD FDPYGFEWLV VDDKERSVLI FVRRDKEGNE
660 670 680 690 700
IIVASNFTPV PRHDYRFGIN QPGKWREILN TDSMHYHGSN AGNGGTVHSD
710 720
EIASHGRQHS LSLTLPPLAT IWLVREAE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M13751 Genomic DNA Translation: AAA23872.1 U18997 Genomic DNA Translation: AAA58230.1 U00096 Genomic DNA Translation: AAC76457.1 AP009048 Genomic DNA Translation: BAE77860.1 |
PIRi | A25498, NQECA |
RefSeqi | NP_417890.1, NC_000913.3 WP_001283723.1, NZ_STEB01000004.1 |
Genome annotation databases
EnsemblBacteriai | AAC76457; AAC76457; b3432 BAE77860; BAE77860; BAE77860 |
GeneIDi | 947940 |
KEGGi | ecj:JW3395 eco:b3432 |
PATRICi | fig|1411691.4.peg.3296 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M13751 Genomic DNA Translation: AAA23872.1 U18997 Genomic DNA Translation: AAA58230.1 U00096 Genomic DNA Translation: AAC76457.1 AP009048 Genomic DNA Translation: BAE77860.1 |
PIRi | A25498, NQECA |
RefSeqi | NP_417890.1, NC_000913.3 WP_001283723.1, NZ_STEB01000004.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1GTY | model | - | A | 24-103 | [»] | |
1M7X | X-ray | 2.30 | A/B/C/D | 113-728 | [»] | |
4LPC | X-ray | 2.39 | A/B/C/D | 117-728 | [»] | |
4LQ1 | X-ray | 2.55 | A/B/C/D | 117-728 | [»] | |
5E6Y | X-ray | 2.60 | A/B/C/D | 117-728 | [»] | |
5E6Z | X-ray | 1.88 | A/B/C/D | 117-728 | [»] | |
5E70 | X-ray | 2.33 | A/B/C/D | 117-728 | [»] | |
SMRi | P07762 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262503, 314 interactors |
IntActi | P07762, 13 interactors |
STRINGi | 511145.b3432 |
Protein family/group databases
CAZyi | CBM48, Carbohydrate-Binding Module Family 48 GH13, Glycoside Hydrolase Family 13 |
Proteomic databases
jPOSTi | P07762 |
PaxDbi | P07762 |
PRIDEi | P07762 |
Genome annotation databases
EnsemblBacteriai | AAC76457; AAC76457; b3432 BAE77860; BAE77860; BAE77860 |
GeneIDi | 947940 |
KEGGi | ecj:JW3395 eco:b3432 |
PATRICi | fig|1411691.4.peg.3296 |
Organism-specific databases
EchoBASEi | EB0373 |
Phylogenomic databases
eggNOGi | COG0296, Bacteria |
HOGENOMi | CLU_004245_3_2_6 |
InParanoidi | P07762 |
PhylomeDBi | P07762 |
Enzyme and pathway databases
UniPathwayi | UPA00164 |
BioCyci | EcoCyc:GLYCOGEN-BRANCH-MONOMER MetaCyc:GLYCOGEN-BRANCH-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P07762 |
PROi | PR:P07762 |
Family and domain databases
CDDi | cd02855, E_set_GBE_prok_N, 1 hit |
Gene3Di | 2.60.40.10, 1 hit 2.60.40.1180, 1 hit |
HAMAPi | MF_00685, GlgB, 1 hit |
InterProi | View protein in InterPro IPR006048, A-amylase/branching_C IPR037439, Branching_enzy IPR006407, GlgB IPR044143, GlgB_N_E_set_prok IPR006047, Glyco_hydro_13_cat_dom IPR004193, Glyco_hydro_13_N IPR013780, Glyco_hydro_b IPR017853, Glycoside_hydrolase_SF IPR013783, Ig-like_fold IPR014756, Ig_E-set |
PANTHERi | PTHR43651, PTHR43651, 1 hit |
Pfami | View protein in Pfam PF00128, Alpha-amylase, 1 hit PF02806, Alpha-amylase_C, 1 hit PF02922, CBM_48, 1 hit |
PIRSFi | PIRSF000463, GlgB, 1 hit |
SMARTi | View protein in SMART SM00642, Aamy, 1 hit |
SUPFAMi | SSF51445, SSF51445, 1 hit SSF81296, SSF81296, 2 hits |
TIGRFAMsi | TIGR01515, branching_enzym, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GLGB_ECOLI | |
Accessioni | P07762Primary (citable) accession number: P07762 Secondary accession number(s): Q2M796 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1988 |
Last sequence update: | August 1, 1988 | |
Last modified: | April 7, 2021 | |
This is version 180 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families