Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Keratin, type II cytoskeletal 4

Gene

Krt4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei397Stutter1

GO - Molecular functioni

  • protein heterodimerization activity Source: UniProtKB
  • structural molecule activity Source: InterPro

GO - Biological processi

  • cytoskeleton organization Source: MGI
  • embryo development ending in birth or egg hatching Source: UniProtKB
  • epithelial cell differentiation Source: UniProtKB
  • negative regulation of epithelial cell proliferation Source: UniProtKB

Enzyme and pathway databases

ReactomeiR-MMU-6805567 Keratinization
R-MMU-6809371 Formation of the cornified envelope

Names & Taxonomyi

Protein namesi
Recommended name:
Keratin, type II cytoskeletal 4
Alternative name(s):
Cytokeratin-4
Short name:
CK-4
Cytoskeletal 57 kDa keratin
Keratin-4
Short name:
K4
Type-II keratin Kb4
Gene namesi
Name:Krt4
Synonyms:Krt2-4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:96701 Krt4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Intermediate filament, Keratin

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000637231 – 525Keratin, type II cytoskeletal 4Add BLAST525

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei13Omega-N-methylarginineCombined sources1

Keywords - PTMi

Methylation

Proteomic databases

PaxDbiP07744
PeptideAtlasiP07744
PRIDEiP07744

PTM databases

iPTMnetiP07744
PhosphoSitePlusiP07744

Expressioni

Tissue specificityi

Expressed in the dorsal and ventral epithelium of the tongue. Highest expression levels are detected in the suprabasal layer with low levels detected in the basal cell layer. Within the suprabasal layer expression is highest in the spinous cells, decreases in the granular cells and is not detected in the stratum corneum.1 Publication

Gene expression databases

BgeeiENSMUSG00000059668
CleanExiMM_KRT4
GenevisibleiP07744 MM

Interactioni

Subunit structurei

Heterotetramer of two type I and two type II keratins. Keratin-4 is generally associated with keratin-13.

GO - Molecular functioni

  • protein heterodimerization activity Source: UniProtKB

Protein-protein interaction databases

BioGridi201034, 2 interactors
IntActiP07744, 2 interactors
MINTiP07744
STRINGi10090.ENSMUSP00000023797

Structurei

3D structure databases

ProteinModelPortaliP07744
SMRiP07744
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini146 – 459IF rodPROSITE-ProRule annotationAdd BLAST314

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 145HeadAdd BLAST145
Regioni146 – 181Coil 1AAdd BLAST36
Regioni182 – 200Linker 1Add BLAST19
Regioni201 – 292Coil 1BAdd BLAST92
Regioni293 – 316Linker 12Add BLAST24
Regioni317 – 455Coil 2Add BLAST139
Regioni456 – 524TailAdd BLAST69

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi10 – 117Gly-richAdd BLAST108
Compositional biasi473 – 510Gly-richAdd BLAST38

Sequence similaritiesi

Belongs to the intermediate filament family.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IGG3 Eukaryota
ENOG410ZE7B LUCA
GeneTreeiENSGT00760000118796
HOGENOMiHOG000230976
HOVERGENiHBG013015
InParanoidiP07744
KOiK07605
OMAiQQCVRGG
OrthoDBiEOG091G09KR
PhylomeDBiP07744
TreeFamiTF317854

Family and domain databases

InterProiView protein in InterPro
IPR001664 IF
IPR018039 IF_conserved
IPR039008 IF_rod_dom
IPR032444 Keratin_2_head
IPR003054 Keratin_II
PANTHERiPTHR23239 PTHR23239, 1 hit
PfamiView protein in Pfam
PF00038 Filament, 1 hit
PF16208 Keratin_2_head, 1 hit
PRINTSiPR01276 TYPE2KERATIN
SMARTiView protein in SMART
SM01391 Filament, 1 hit
PROSITEiView protein in PROSITE
PS00226 IF_ROD_1, 1 hit
PS51842 IF_ROD_2, 1 hit

Sequencei

Sequence statusi: Complete.

P07744-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIARQSSVRG ASRGFSSGSA IAGGVKRVAF SSGSMSGGAG RCSSGGFGSR
60 70 80 90 100
SLYNLGGHKS ISMSVAGSCQ GGGYGGAGGF GVGGYGAGFG AGGFGGGFGG
110 120 130 140 150
SFNGRGGPGF PVCPAGGIQE VTINQSLLTP LQVEIDPEIQ KIRTAEREQI
160 170 180 190 200
KTLNNKFASF IDKVRFLEQQ NKVLETKWNL LQQQTTTTSP KSLDPFFETY
210 220 230 240 250
INALRKNLDT LSNDKGRLQS ELKMMQDSVE DFKTKYEEEI NKRTAAENDF
260 270 280 290 300
VVLKKDVDAA YMIKVELEAK MESLKDEINF TRVLYEAELA QMQTHVSDTS
310 320 330 340 350
VVLSMDNNRN LDLDGIIAEV RAQYEDIARK SKAEVESWYQ IKVQQLQMSA
360 370 380 390 400
DQHGDSLKTT KNEISELNRM IQRLRAEIEN IKKQSQTLQA SVADAEQRGE
410 420 430 440 450
LALKDAYSKR AELETALQKA KEDLARLLRD YQALMNVKLA LDVEIATYRK
460 470 480 490 500
LLEGEECRMS GECKSAVSIS VVGGSASIGG SGLGLGSGFC SGSGSGSGFG
510 520
FGGGIYGGSG SKITSSATIT KRSPR
Length:525
Mass (Da):56,283
Last modified:November 22, 2005 - v2
Checksum:i27A3A3DF7E87DF90
GO

Sequence cautioni

The sequence AAH64008 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti13R → P in CAA27207 (PubMed:2418416).Curated1
Sequence conflicti16S → T in CAA27207 (PubMed:2418416).Curated1
Sequence conflicti79G → D in AAH64008 (PubMed:15489334).Curated1
Sequence conflicti388L → P in CAA27207 (PubMed:2418416).Curated1
Sequence conflicti476 – 480ASIGG → QHWR in CAA27207 (PubMed:2418416).Curated5

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03491 mRNA Translation: CAA27207.1
BC064008 mRNA Translation: AAH64008.1 Different initiation.
BC145839 mRNA Translation: AAI45840.1
BC145841 mRNA Translation: AAI45842.1
CCDSiCCDS37222.1
PIRiA23518
RefSeqiNP_032501.2, NM_008475.2
UniGeneiMm.46425

Genome annotation databases

EnsembliENSMUST00000023797; ENSMUSP00000023797; ENSMUSG00000059668
GeneIDi16682
KEGGimmu:16682
UCSCiuc007xuf.1 mouse

Similar proteinsi

Entry informationi

Entry nameiK2C4_MOUSE
AccessioniPrimary (citable) accession number: P07744
Secondary accession number(s): A6H6D6, Q6P3F5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: November 22, 2005
Last modified: May 23, 2018
This is version 139 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health