UniProtKB - P07688 (CATB_BOVIN)
Cathepsin B
CTSB
Functioni
Thiol protease which is believed to participate in intracellular degradation and turnover of proteins (PubMed:1856234).
Cleaves matrix extracellular phosphoglycoprotein MEPE (By similarity).
Involved in the solubilization of cross-linked TG/thyroglobulin in the thyroid follicle lumen (By similarity).
Has also been implicated in tumor invasion and metastasis (By similarity).
By similarity1 PublicationCatalytic activityi
- Hydrolysis of proteins with broad specificity for peptide bonds. Preferentially cleaves -Arg-Arg-|-Xaa bonds in small molecule substrates (thus differing from cathepsin L). In addition to being an endopeptidase, shows peptidyl-dipeptidase activity, liberating C-terminal dipeptides.1 Publication EC:3.4.22.1
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 108 | PROSITE-ProRule annotation2 Publications | 1 | |
Active sitei | 278 | PROSITE-ProRule annotation1 Publication | 1 | |
Active sitei | 298 | PROSITE-ProRule annotation1 Publication | 1 |
GO - Molecular functioni
- collagen binding Source: Ensembl
- cysteine-type endopeptidase activity Source: GO_Central
- endopeptidase activity Source: UniProtKB
- proteoglycan binding Source: Ensembl
GO - Biological processi
- cellular response to thyroid hormone stimulus Source: Ensembl
- collagen catabolic process Source: Ensembl
- decidualization Source: Ensembl
- epithelial cell differentiation Source: Ensembl
- negative regulation of peptidase activity Source: Ensembl
- proteolysis involved in cellular protein catabolic process Source: GO_Central
- thyroid hormone generation Source: Ensembl
- viral entry into host cell Source: Ensembl
Keywordsi
Molecular function | Hydrolase, Protease, Thiol protease |
Enzyme and pathway databases
BRENDAi | 3.4.22.1, 908 |
Reactomei | R-BTA-2132295, MHC class II antigen presentation R-BTA-6798695, Neutrophil degranulation |
Protein family/group databases
MEROPSi | C01.060 |
Names & Taxonomyi
Protein namesi | Recommended name: Cathepsin B (EC:3.4.22.11 Publication)Alternative name(s): BCSB Cleaved into the following 2 chains: Cathepsin B light chain2 Publications Cathepsin B heavy chain2 Publications |
Gene namesi | Name:CTSB |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Organism-specific databases
VEuPathDBi | HostDB:ENSBTAG00000012442 |
VGNCi | VGNC:27813, CTSB |
Subcellular locationi
Lysosome
- Lysosome 1 Publication
Plasma membrane
- Apical cell membrane By similarity; Peripheral membrane protein By similarity; Extracellular side By similarity
Extracellular region or secreted
- extracellular space By similarity
Other locations
- Melanosome By similarity
Note: Localizes to the lumen of thyroid follicles and to the apical membrane of thyroid epithelial cells.By similarity
Extracellular region or secreted
- extracellular space Source: GO_Central
Lysosome
- lysosome Source: GO_Central
Plasma Membrane
- apical plasma membrane Source: UniProtKB-SubCell
- external side of plasma membrane Source: Ensembl
Other locations
- melanosome Source: UniProtKB-SubCell
- peptidase inhibitor complex Source: Ensembl
- perinuclear region of cytoplasm Source: Ensembl
Keywords - Cellular componenti
Cell membrane, Lysosome, Membrane, SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 17 | Sequence analysisAdd BLAST | 17 | |
PropeptideiPRO_0000026138 | 18 – 79 | Activation peptideAdd BLAST | 62 | |
ChainiPRO_0000026139 | 80 – 332 | Cathepsin B1 PublicationAdd BLAST | 253 | |
ChainiPRO_0000026140 | 80 – 126 | Cathepsin B light chain2 PublicationsAdd BLAST | 47 | |
ChainiPRO_0000026141 | 129 – 332 | Cathepsin B heavy chain2 PublicationsAdd BLAST | 204 | |
PropeptideiPRO_0000026142 | 333 – 335 | By similarity | 3 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 93 ↔ 122 | 3 Publications | ||
Disulfide bondi | 105 ↔ 150 | 3 Publications | ||
Disulfide bondi | 141 ↔ 207 | 2 Publications | ||
Disulfide bondi | 142 ↔ 146 | 2 Publications | ||
Disulfide bondi | 179 ↔ 211 | 2 Publications | ||
Disulfide bondi | 187 ↔ 198 | 2 Publications | ||
Glycosylationi | 192 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Modified residuei | 220 | N6-acetyllysineBy similarity | 1 | |
Disulfide bondi | 227 ↔ 331 | 3 Publications |
Keywords - PTMi
Acetylation, Disulfide bond, Glycoprotein, ZymogenProteomic databases
PaxDbi | P07688 |
PeptideAtlasi | P07688 |
PRIDEi | P07688 |
PTM databases
CarbonylDBi | P07688 |
iPTMneti | P07688 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSBTAG00000012442, Expressed in placenta and 92 other tissues |
Interactioni
Subunit structurei
Dimer of a heavy chain and a light chain cross-linked by a disulfide bond.
Interacts with SRPX2. Directly interacts with SHKBP1.
By similarityGO - Molecular functioni
- proteoglycan binding Source: Ensembl
Protein-protein interaction databases
BioGRIDi | 158468, 1 interactor |
STRINGi | 9913.ENSBTAP00000036650 |
Chemistry databases
BindingDBi | P07688 |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P07688 |
SMRi | P07688 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P07688 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG1543, Eukaryota |
GeneTreei | ENSGT00940000158680 |
HOGENOMi | CLU_012184_3_3_1 |
InParanoidi | P07688 |
OMAi | DEKIPYW |
OrthoDBi | 865289at2759 |
TreeFami | TF314576 |
Family and domain databases
InterProi | View protein in InterPro IPR038765, Papain-like_cys_pep_sf IPR025661, Pept_asp_AS IPR000169, Pept_cys_AS IPR025660, Pept_his_AS IPR000668, Peptidase_C1A_C IPR012599, Propeptide_C1A |
Pfami | View protein in Pfam PF00112, Peptidase_C1, 1 hit PF08127, Propeptide_C1, 1 hit |
PRINTSi | PR00705, PAPAIN |
SMARTi | View protein in SMART SM00645, Pept_C1, 1 hit |
SUPFAMi | SSF54001, SSF54001, 1 hit |
PROSITEi | View protein in PROSITE PS00640, THIOL_PROTEASE_ASN, 1 hit PS00139, THIOL_PROTEASE_CYS, 1 hit PS00639, THIOL_PROTEASE_HIS, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MWRLLATLSC LLVLTSARSS LYFPPLSDEL VNFVNKQNTT WKAGHNFYNV
60 70 80 90 100
DLSYVKKLCG AILGGPKLPQ RDAFAADVVL PESFDAREQW PNCPTIKEIR
110 120 130 140 150
DQGSCGSCWA FGAVEAISDR ICIHSNGRVN VEVSAEDMLT CCGGECGDGC
160 170 180 190 200
NGGFPSGAWN FWTKKGLVSG GLYNSHVGCR PYSIPPCEHH VNGSRPPCTG
210 220 230 240 250
EGDTPKCSKT CEPGYSPSYK EDKHFGCSSY SVANNEKEIM AEIYKNGPVE
260 270 280 290 300
GAFSVYSDFL LYKSGVYQHV SGEIMGGHAI RILGWGVENG TPYWLVGNSW
310 320 330
NTDWGDNGFF KILRGQDHCG IESEIVAGMP CTHQY
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 143 | G → D in AAI02998 (Ref. 3) Curated | 1 | |
Sequence conflicti | 154 | F → E AA sequence (PubMed:3379063).Curated | 1 | |
Sequence conflicti | 208 | S → N AA sequence (PubMed:3379063).Curated | 1 | |
Sequence conflicti | 297 | G → A AA sequence (PubMed:3379063).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L06075 mRNA Translation: AAA03064.1 M64620 mRNA Translation: AAA30434.1 U16336 , U16337, U16338, U16339, U16341, U16342, U16343 Genomic DNA Translation: AAA80198.1 BC102997 mRNA Translation: AAI02998.1 |
PIRi | S38328, KHBOB |
RefSeqi | NP_776456.1, NM_174031.2 XP_005209879.1, XM_005209822.3 XP_010805998.1, XM_010807696.2 |
Genome annotation databases
Ensembli | ENSBTAT00000036795; ENSBTAP00000036650; ENSBTAG00000012442 |
GeneIDi | 281105 |
KEGGi | bta:281105 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L06075 mRNA Translation: AAA03064.1 M64620 mRNA Translation: AAA30434.1 U16336 , U16337, U16338, U16339, U16341, U16342, U16343 Genomic DNA Translation: AAA80198.1 BC102997 mRNA Translation: AAI02998.1 |
PIRi | S38328, KHBOB |
RefSeqi | NP_776456.1, NM_174031.2 XP_005209879.1, XM_005209822.3 XP_010805998.1, XM_010807696.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1ITO | X-ray | 2.29 | A | 80-335 | [»] | |
1QDQ | X-ray | 2.18 | A | 80-332 | [»] | |
1SP4 | X-ray | 2.20 | A | 80-127 | [»] | |
B | 128-332 | [»] | ||||
2DC6 | X-ray | 2.30 | A | 80-335 | [»] | |
2DC7 | X-ray | 1.94 | A | 80-335 | [»] | |
2DC8 | X-ray | 1.94 | A | 80-335 | [»] | |
2DC9 | X-ray | 1.94 | A | 80-335 | [»] | |
2DCA | X-ray | 2.11 | A | 80-335 | [»] | |
2DCB | X-ray | 1.94 | A | 80-335 | [»] | |
2DCC | X-ray | 1.93 | A | 80-335 | [»] | |
2DCD | X-ray | 2.50 | A | 80-335 | [»] | |
AlphaFoldDBi | P07688 | |||||
SMRi | P07688 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 158468, 1 interactor |
STRINGi | 9913.ENSBTAP00000036650 |
Chemistry databases
BindingDBi | P07688 |
ChEMBLi | CHEMBL2323 |
Protein family/group databases
MEROPSi | C01.060 |
PTM databases
CarbonylDBi | P07688 |
iPTMneti | P07688 |
Proteomic databases
PaxDbi | P07688 |
PeptideAtlasi | P07688 |
PRIDEi | P07688 |
Genome annotation databases
Ensembli | ENSBTAT00000036795; ENSBTAP00000036650; ENSBTAG00000012442 |
GeneIDi | 281105 |
KEGGi | bta:281105 |
Organism-specific databases
CTDi | 1508 |
VEuPathDBi | HostDB:ENSBTAG00000012442 |
VGNCi | VGNC:27813, CTSB |
Phylogenomic databases
eggNOGi | KOG1543, Eukaryota |
GeneTreei | ENSGT00940000158680 |
HOGENOMi | CLU_012184_3_3_1 |
InParanoidi | P07688 |
OMAi | DEKIPYW |
OrthoDBi | 865289at2759 |
TreeFami | TF314576 |
Enzyme and pathway databases
BRENDAi | 3.4.22.1, 908 |
Reactomei | R-BTA-2132295, MHC class II antigen presentation R-BTA-6798695, Neutrophil degranulation |
Miscellaneous databases
EvolutionaryTracei | P07688 |
PROi | PR:P07688 |
Gene expression databases
Bgeei | ENSBTAG00000012442, Expressed in placenta and 92 other tissues |
Family and domain databases
InterProi | View protein in InterPro IPR038765, Papain-like_cys_pep_sf IPR025661, Pept_asp_AS IPR000169, Pept_cys_AS IPR025660, Pept_his_AS IPR000668, Peptidase_C1A_C IPR012599, Propeptide_C1A |
Pfami | View protein in Pfam PF00112, Peptidase_C1, 1 hit PF08127, Propeptide_C1, 1 hit |
PRINTSi | PR00705, PAPAIN |
SMARTi | View protein in SMART SM00645, Pept_C1, 1 hit |
SUPFAMi | SSF54001, SSF54001, 1 hit |
PROSITEi | View protein in PROSITE PS00640, THIOL_PROTEASE_ASN, 1 hit PS00139, THIOL_PROTEASE_CYS, 1 hit PS00639, THIOL_PROTEASE_HIS, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CATB_BOVIN | |
Accessioni | P07688Primary (citable) accession number: P07688 Secondary accession number(s): Q3ZC03 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1988 |
Last sequence update: | May 30, 2006 | |
Last modified: | May 25, 2022 | |
This is version 182 of the entry and version 5 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families