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Protein

Glucoamylase 1

Gene
N/A
Organism
Rhizopus oryzae (Mucormycosis agent) (Rhizopus arrhizus var. delemar)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Rhizopus glucoamylase exists in multiple forms, Gluc 1, Gluc 2, and Gluc 3, all of which hydrolyze gelatinized starch at similar rates, but only the largest one (Gluc 1) is able to adsorb raw starch.
Glucoamylase 3 may be 110-604 instead of 116-104.

Catalytic activityi

Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei279SubstrateBy similarity1
Active sitei336Proton acceptorPROSITE-ProRule annotation1
Active sitei339Proton donorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Polysaccharide degradation

Enzyme and pathway databases

BRENDAi3.2.1.3 5365

Protein family/group databases

CAZyiCBM21 Carbohydrate-Binding Module Family 21
GH15 Glycoside Hydrolase Family 15
mycoCLAPiGLA15E_RHIOR

Names & Taxonomyi

Protein namesi
Recommended name:
Glucoamylase 1 (EC:3.2.1.3)
Short name:
Gluc 1
Alternative name(s):
1,4-alpha-D-glucan glucohydrolase
Glucan 1,4-alpha-glucosidase
Cleaved into the following 2 chains:
Glucoamylase 2
Short name:
Gluc 2
Glucoamylase 3
Short name:
Gluc 3
OrganismiRhizopus oryzae (Mucormycosis agent) (Rhizopus arrhizus var. delemar)
Taxonomic identifieri64495 [NCBI]
Taxonomic lineageiEukaryotaFungiFungi incertae sedisMucoromycotaMucoromycotinaMucoromycetesMucoralesMucorineaeRhizopodaceaeRhizopus

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4449

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Add BLAST25
ChainiPRO_000000147226 – 604Glucoamylase 1Add BLAST579
ChainiPRO_0000001473116 – 604Glucoamylase 3Add BLAST489
ChainiPRO_0000001474159 – 604Glucoamylase 2Add BLAST446

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi122N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi167N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi230N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi236N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi564N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiP07683

Interactioni

Chemistry databases

BindingDBiP07683

Structurei

Secondary structure

1604
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi30 – 44Combined sources15
Beta strandi46 – 52Combined sources7
Beta strandi55 – 57Combined sources3
Beta strandi59 – 67Combined sources9
Beta strandi78 – 80Combined sources3
Beta strandi82 – 86Combined sources5
Beta strandi93 – 99Combined sources7
Beta strandi102 – 115Combined sources14
Beta strandi117 – 120Combined sources4
Beta strandi122 – 125Combined sources4

3D structure databases

ProteinModelPortaliP07683
SMRiP07683
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07683

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 130CBM21PROSITE-ProRule annotationAdd BLAST105

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni26 – 115Adsorption to raw starchAdd BLAST90
Regioni116 – 604Starch degradationAdd BLAST489

Sequence similaritiesi

Belongs to the glycosyl hydrolase 15 family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di1.50.10.10, 1 hit
2.60.40.2440, 2 hits
InterProiView protein in InterPro
IPR008928 6-hairpin_glycosidase_sf
IPR012341 6hp_glycosidase-like_sf
IPR005036 CBM21_dom
IPR038175 CBM21_dom_sf
IPR011613 GH15-like
IPR000165 Glucoamylase
PfamiView protein in Pfam
PF03370 CBM_21, 1 hit
PF00723 Glyco_hydro_15, 1 hit
PRINTSiPR00736 GLHYDRLASE15
SUPFAMiSSF48208 SSF48208, 1 hit
PROSITEiView protein in PROSITE
PS51159 CBM21, 1 hit
PS00820 GLUCOAMYLASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07683-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQLFNLPLKV SFFLVLSYFS LLVSAASIPS SASVQLDSYN YDGSTFSGKI
60 70 80 90 100
YVKNIAYSKK VTVIYADGSD NWNNNGNTIA ASYSAPISGS NYEYWTFSAS
110 120 130 140 150
INGIKEFYIK YEVSGKTYYD NNNSANYQVS TSKPTTTTAT ATTTTAPSTS
160 170 180 190 200
TTTPPSRSEP ATFPTGNSTI SSWIKKQEGI SRFAMLRNIN PPGSATGFIA
210 220 230 240 250
ASLSTAGPDY YYAWTRDAAL TSNVIVYEYN TTLSGNKTIL NVLKDYVTFS
260 270 280 290 300
VKTQSTSTVC NCLGEPKFNP DASGYTGAWG RPQNDGPAER ATTFILFADS
310 320 330 340 350
YLTQTKDASY VTGTLKPAIF KDLDYVVNVW SNGCFDLWEE VNGVHFYTLM
360 370 380 390 400
VMRKGLLLGA DFAKRNGDST RASTYSSTAS TIANKISSFW VSSNNWIQVS
410 420 430 440 450
QSVTGGVSKK GLDVSTLLAA NLGSVDDGFF TPGSEKILAT AVAVEDSFAS
460 470 480 490 500
LYPINKNLPS YLGNSIGRYP EDTYNGNGNS QGNSWFLAVT GYAELYYRAI
510 520 530 540 550
KEWIGNGGVT VSSISLPFFK KFDSSATSGK KYTVGTSDFN NLAQNIALAA
560 570 580 590 600
DRFLSTVQLH AHNNGSLAEE FDRTTGLSTG ARDLTWSHAS LITASYAKAG

APAA
Length:604
Mass (Da):65,162
Last modified:November 1, 1995 - v2
Checksum:i78421F1AAA93ADB9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00049 Genomic DNA Translation: BAA00033.1
PIRiJP0001

Similar proteinsi

Entry informationi

Entry nameiAMYG_RHIOR
AccessioniPrimary (citable) accession number: P07683
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: November 1, 1995
Last modified: July 18, 2018
This is version 109 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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