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Protein

Submandibular glandular kallikrein-9

Gene

Klk9

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin. This enzyme has a vasoconstrictor activity. KLK-9 has both a chymotrypsin-like and a trypsin-like properties.

Catalytic activityi

Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei63Charge relay system1
Active sitei118Charge relay system1
Active sitei211Charge relay system1

GO - Molecular functioni

GO - Biological processi

  • positive regulation of vasoconstriction Source: RGD
  • regulation of systemic arterial blood pressure Source: GO_Central
  • zymogen activation Source: GO_Central

Keywordsi

Molecular functionHydrolase, Protease, Serine protease

Enzyme and pathway databases

BRENDAi3.4.21.B40 5301

Protein family/group databases

MEROPSiS01.407

Names & Taxonomyi

Protein namesi
Recommended name:
Submandibular glandular kallikrein-9 (EC:3.4.21.35)
Short name:
rGK-9
Alternative name(s):
KLK-S3
S3 kallikrein
Submandibular enzymatic vasoconstrictor
Short name:
SEV
Tissue kallikrein
Cleaved into the following 2 chains:
Gene namesi
Name:Klk9
Synonyms:Klk-9, Klks3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi727805 Klks3

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18CuratedAdd BLAST18
PropeptideiPRO_000002800919 – 24Activation peptide3 Publications6
ChainiPRO_000002801025 – 259Submandibular glandular kallikrein-9Add BLAST235
ChainiPRO_000002801125 – 111Submandibular glandular kallikrein-9 light chainAdd BLAST87
ChainiPRO_0000028012112 – 259Submandibular glandular kallikrein-9 heavy chainAdd BLAST148

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi31 ↔ 171PROSITE-ProRule annotation
Disulfide bondi48 ↔ 64PROSITE-ProRule annotation
Glycosylationi106N-linked (GlcNAc...) asparagineCurated1
Disulfide bondi150 ↔ 217PROSITE-ProRule annotation
Disulfide bondi182 ↔ 196PROSITE-ProRule annotation
Disulfide bondi207 ↔ 232PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiP07647
PRIDEiP07647

Expressioni

Gene expression databases

BgeeiENSRNOG00000032857 Expressed in 9 organ(s), highest expression level in adult mammalian kidney
ExpressionAtlasiP07647 baseline and differential
GenevisibleiP07647 RN

Interactioni

Subunit structurei

Heterodimer of a light chain and heavy chain linked by a disulfide bond.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000025723

Structurei

3D structure databases

ProteinModelPortaliP07647
SMRiP07647
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 256Peptidase S1PROSITE-ProRule annotationAdd BLAST232

Sequence similaritiesi

Belongs to the peptidase S1 family. Kallikrein subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627 Eukaryota
COG5640 LUCA
GeneTreeiENSGT00930000150914
HOGENOMiHOG000251820
HOVERGENiHBG013304
InParanoidiP07647
KOiK01325
PhylomeDBiP07647
TreeFamiTF331065

Family and domain databases

CDDicd00190 Tryp_SPc, 1 hit
InterProiView protein in InterPro
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR001254 Trypsin_dom
IPR018114 TRYPSIN_HIS
IPR033116 TRYPSIN_SER
PfamiView protein in Pfam
PF00089 Trypsin, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
PROSITEiView protein in PROSITE
PS50240 TRYPSIN_DOM, 1 hit
PS00134 TRYPSIN_HIS, 1 hit
PS00135 TRYPSIN_SER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07647-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MWFLILFLAL SLGQIDAAPP GQSRVVGGYN CETNSQPWQV AVIGTTFCGG
60 70 80 90 100
VLIDPSWVIT AAHCYSKNYR VLLGRNNLVK DEPFAQRRLV SQSFQHPDYI
110 120 130 140 150
PVFMRNHTRQ RAYDHNNDLM LLHLSKPADI TGGVKVIDLP TEEPKVGSIC
160 170 180 190 200
LASGWGMTNP SEMKLSHDLQ CVNIHLLSNE KCIETYKNIE TDVTLCAGEM
210 220 230 240 250
DGGKDTCTGD SGGPLICDGV LQGLTSGGAT PCAKPKTPAI YAKLIKFTSW

IKKVMKENP
Length:259
Mass (Da):28,368
Last modified:April 1, 1988 - v1
Checksum:iD167E8518BEC0791
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11566 mRNA Translation: AAA41467.1
BC061771 mRNA Translation: AAH61771.1
PIRiD23863
RefSeqiNP_786935.1, NM_175759.2
UniGeneiRn.224461

Genome annotation databases

EnsembliENSRNOT00000025723; ENSRNOP00000025723; ENSRNOG00000032857
GeneIDi292868
KEGGirno:292868
UCSCiRGD:727805 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11566 mRNA Translation: AAA41467.1
BC061771 mRNA Translation: AAH61771.1
PIRiD23863
RefSeqiNP_786935.1, NM_175759.2
UniGeneiRn.224461

3D structure databases

ProteinModelPortaliP07647
SMRiP07647
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000025723

Protein family/group databases

MEROPSiS01.407

Proteomic databases

PaxDbiP07647
PRIDEiP07647

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000025723; ENSRNOP00000025723; ENSRNOG00000032857
GeneIDi292868
KEGGirno:292868
UCSCiRGD:727805 rat

Organism-specific databases

CTDi292868
RGDi727805 Klks3

Phylogenomic databases

eggNOGiKOG3627 Eukaryota
COG5640 LUCA
GeneTreeiENSGT00930000150914
HOGENOMiHOG000251820
HOVERGENiHBG013304
InParanoidiP07647
KOiK01325
PhylomeDBiP07647
TreeFamiTF331065

Enzyme and pathway databases

BRENDAi3.4.21.B40 5301

Miscellaneous databases

PROiPR:P07647

Gene expression databases

BgeeiENSRNOG00000032857 Expressed in 9 organ(s), highest expression level in adult mammalian kidney
ExpressionAtlasiP07647 baseline and differential
GenevisibleiP07647 RN

Family and domain databases

CDDicd00190 Tryp_SPc, 1 hit
InterProiView protein in InterPro
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR001254 Trypsin_dom
IPR018114 TRYPSIN_HIS
IPR033116 TRYPSIN_SER
PfamiView protein in Pfam
PF00089 Trypsin, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
PROSITEiView protein in PROSITE
PS50240 TRYPSIN_DOM, 1 hit
PS00134 TRYPSIN_HIS, 1 hit
PS00135 TRYPSIN_SER, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiKLK9_RAT
AccessioniPrimary (citable) accession number: P07647
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: November 7, 2018
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Peptidase families
    Classification of peptidase families and list of entries
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Main funding by: National Institutes of Health

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