UniProtKB - P07623 (METAS_ECOLI)
Homoserine O-succinyltransferase
metAS
Functioni
Transfers a succinyl group from succinyl-CoA to L-homoserine, forming succinyl-L-homoserine (PubMed:10572016, PubMed:17442255, PubMed:17302437, PubMed:28581482).
Utilizes a ping-pong kinetic mechanism in which the succinyl group of succinyl-CoA is initially transferred to the enzyme to form a succinyl-enzyme intermediate before subsequent transfer to homoserine to form the final product, O-succinylhomoserine (PubMed:10572016, PubMed:17442255, PubMed:17302437).
Cannot use acetyl-CoA (PubMed:10572016).
4 PublicationsCatalytic activityi
- EC:2.3.1.46UniRule annotation4 Publications
Activity regulationi
Kineticsi
- KM=0.17 mM for succinyl-CoA1 Publication
- KM=0.13 mM for succinyl-CoA1 Publication
- KM=0.28 mM for succinyl-CoA1 Publication
- KM=1.6 mM for L-homoserine1 Publication
- KM=0.72 mM for L-homoserine1 Publication
- KM=0.38 mM for L-homoserine1 Publication
- KM=0.64 mM for CoA1 Publication
- KM=3.5 mM for O-succinylhomoserine1 Publication
: L-methionine biosynthesis via de novo pathway Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes O-succinyl-L-homoserine from L-homoserine.UniRule annotation1 Publication This subpathway is part of the pathway L-methionine biosynthesis via de novo pathway, which is itself part of Amino-acid biosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes O-succinyl-L-homoserine from L-homoserine, the pathway L-methionine biosynthesis via de novo pathway and in Amino-acid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 111 | Important for acyl-CoA specificityUniRule annotation | 1 | |
Active sitei | 142 | Acyl-thioester intermediateUniRule annotation3 Publications | 1 | |
Binding sitei | 163 | SubstrateUniRule annotation | 1 | |
Binding sitei | 192 | SubstrateUniRule annotation | 1 | |
Sitei | 192 | Important for substrate specificityUniRule annotation | 1 | |
Active sitei | 235 | Proton acceptorUniRule annotation2 Publications | 1 | |
Active sitei | 237 | UniRule annotation2 Publications | 1 | |
Binding sitei | 249 | SubstrateUniRule annotation | 1 |
GO - Molecular functioni
- homoserine O-acetyltransferase activity Source: UniProtKB-UniRule
- homoserine O-succinyltransferase activity Source: EcoCyc
GO - Biological processi
- L-methionine biosynthetic process from homoserine via O-succinyl-L-homoserine and cystathionine Source: InterPro
Keywordsi
Molecular function | Acyltransferase, Transferase |
Biological process | Amino-acid biosynthesis, Methionine biosynthesis |
Enzyme and pathway databases
BioCyci | EcoCyc:HOMSUCTRAN-MONOMER |
BRENDAi | 2.3.1.46, 2026 |
SABIO-RKi | P07623 |
UniPathwayi | UPA00051;UER00075 |
Names & Taxonomyi
Protein namesi | Recommended name: Homoserine O-succinyltransferase1 PublicationUniRule annotation (EC:2.3.1.464 Publications)Short name: HST1 PublicationUniRule annotation Alternative name(s): Homoserine transsuccinylase1 PublicationUniRule annotation Short name: HTS1 PublicationUniRule annotation |
Gene namesi | Name:metAS1 PublicationUniRule annotation Synonyms:metA1 Publication Ordered Locus Names:b4013, JW3973 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotationCurated
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 46 – 47 | KK → AA: Lack of activity. 3 Publications | 2 | |
Mutagenesisi | 46 | K → A: 16-fold decrease in Km for L-homoserine. 32-fold decrease in catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 46 | K → L: 6-fold increase in Km for L-homoserine and in Km for succinyl-CoA. Slight increase in catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 46 | K → R: Slight decrease in catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 47 | K → A: 22-fold decrease in Km for L-homoserine. Almost loss of catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 47 | K → L: 8-fold decrease in Km for L-homoserine and 10-fold increase in Km for succinyl-CoA. 20-fold decrease in catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 47 | K → R: 14-fold decrease in Km for L-homoserine. 72-fold decrease in catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 90 | C → A or S: No change in activity. 1 Publication | 1 | |
Mutagenesisi | 142 | C → A or S: Lack of activity. 2 Publications | 1 | |
Mutagenesisi | 157 | K → L: Lack of activity. 1 Publication | 1 | |
Mutagenesisi | 193 | R → A: 3-fold increase in Km for L-homoserine. 8-fold decrease in catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 193 | R → K: 8-fold decrease in catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 235 | H → A or N: Lack of activity. 2 Publications | 1 | |
Mutagenesisi | 237 | E → A: 32-fold decrease in catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 237 | E → D: 3-fold decrease in catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 238 | Y → F: 5-fold increase in Km for L-homoserine. 1 Publication | 1 | |
Mutagenesisi | 246 | E → A: 150-fold increase in Km for L-homoserine. 1 Publication | 1 | |
Mutagenesisi | 246 | E → D: 24-fold increase in Km for L-homoserine. 1 Publication | 1 | |
Mutagenesisi | 249 | R → K: 4-fold increase in Km for L-homoserine. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000199748 | 2 – 309 | Homoserine O-succinyltransferaseAdd BLAST | 308 |
Proteomic databases
PaxDbi | P07623 |
PRIDEi | P07623 |
Expressioni
Inductioni
Interactioni
Subunit structurei
Homodimer.
UniRule annotation1 PublicationProtein-protein interaction databases
BioGRIDi | 4259289, 20 interactors |
IntActi | P07623, 2 interactors |
MINTi | P07623 |
STRINGi | 511145.b4013 |
Structurei
Secondary structure
3D structure databases
SMRi | P07623 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG1897, Bacteria |
HOGENOMi | CLU_057851_0_1_6 |
InParanoidi | P07623 |
PhylomeDBi | P07623 |
Family and domain databases
CDDi | cd03131, GATase1_HTS, 1 hit |
Gene3Di | 3.40.50.880, 1 hit |
HAMAPi | MF_00295, MetA_acyltransf, 1 hit |
InterProi | View protein in InterPro IPR029062, Class_I_gatase-like IPR005697, HST_MetA IPR033752, MetA_family |
PANTHERi | PTHR20919, PTHR20919, 1 hit |
Pfami | View protein in Pfam PF04204, HTS, 1 hit |
PIRSFi | PIRSF000450, H_ser_succinyltr, 1 hit |
SUPFAMi | SSF52317, SSF52317, 1 hit |
TIGRFAMsi | TIGR01001, metA, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MPIRVPDELP AVNFLREENV FVMTTSRASG QEIRPLKVLI LNLMPKKIET
60 70 80 90 100
ENQFLRLLSN SPLQVDIQLL RIDSRESRNT PAEHLNNFYC NFEDIQDQNF
110 120 130 140 150
DGLIVTGAPL GLVEFNDVAY WPQIKQVLEW SKDHVTSTLF VCWAVQAALN
160 170 180 190 200
ILYGIPKQTR TEKLSGVYEH HILHPHALLT RGFDDSFLAP HSRYADFPAA
210 220 230 240 250
LIRDYTDLEI LAETEEGDAY LFASKDKRIA FVTGHPEYDA QTLAQEFFRD
260 270 280 290 300
VEAGLDPDVP YNYFPHNDPQ NTPRASWRSH GNLLFTNWLN YYVYQITPYD
LRHMNPTLD
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 67 | I → V (PubMed:2654885).Curated | 1 | |
Sequence conflicti | 67 | I → V (PubMed:6094503).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X14501 Genomic DNA Translation: CAA32654.1 U00006 Genomic DNA Translation: AAC43107.1 U00096 Genomic DNA Translation: AAC76983.1 AP009048 Genomic DNA Translation: BAE78015.1 M10210 Genomic DNA Translation: AAA24157.1 X12431 Genomic DNA No translation available. |
PIRi | D65208, XYECM |
RefSeqi | NP_418437.1, NC_000913.3 WP_001122779.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC76983; AAC76983; b4013 BAE78015; BAE78015; BAE78015 |
GeneIDi | 948513 |
KEGGi | ecj:JW3973 eco:b4013 |
PATRICi | fig|1411691.4.peg.2700 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X14501 Genomic DNA Translation: CAA32654.1 U00006 Genomic DNA Translation: AAC43107.1 U00096 Genomic DNA Translation: AAC76983.1 AP009048 Genomic DNA Translation: BAE78015.1 M10210 Genomic DNA Translation: AAA24157.1 X12431 Genomic DNA No translation available. |
PIRi | D65208, XYECM |
RefSeqi | NP_418437.1, NC_000913.3 WP_001122779.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6MTG | X-ray | 1.85 | A/B | 1-296 | [»] | |
7CBE | X-ray | 1.70 | A/B | 1-309 | [»] | |
SMRi | P07623 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259289, 20 interactors |
IntActi | P07623, 2 interactors |
MINTi | P07623 |
STRINGi | 511145.b4013 |
Proteomic databases
PaxDbi | P07623 |
PRIDEi | P07623 |
Genome annotation databases
EnsemblBacteriai | AAC76983; AAC76983; b4013 BAE78015; BAE78015; BAE78015 |
GeneIDi | 948513 |
KEGGi | ecj:JW3973 eco:b4013 |
PATRICi | fig|1411691.4.peg.2700 |
Organism-specific databases
EchoBASEi | EB0576 |
Phylogenomic databases
eggNOGi | COG1897, Bacteria |
HOGENOMi | CLU_057851_0_1_6 |
InParanoidi | P07623 |
PhylomeDBi | P07623 |
Enzyme and pathway databases
UniPathwayi | UPA00051;UER00075 |
BioCyci | EcoCyc:HOMSUCTRAN-MONOMER |
BRENDAi | 2.3.1.46, 2026 |
SABIO-RKi | P07623 |
Miscellaneous databases
PROi | PR:P07623 |
Family and domain databases
CDDi | cd03131, GATase1_HTS, 1 hit |
Gene3Di | 3.40.50.880, 1 hit |
HAMAPi | MF_00295, MetA_acyltransf, 1 hit |
InterProi | View protein in InterPro IPR029062, Class_I_gatase-like IPR005697, HST_MetA IPR033752, MetA_family |
PANTHERi | PTHR20919, PTHR20919, 1 hit |
Pfami | View protein in Pfam PF04204, HTS, 1 hit |
PIRSFi | PIRSF000450, H_ser_succinyltr, 1 hit |
SUPFAMi | SSF52317, SSF52317, 1 hit |
TIGRFAMsi | TIGR01001, metA, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | METAS_ECOLI | |
Accessioni | P07623Primary (citable) accession number: P07623 Secondary accession number(s): Q2M6U1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1988 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 174 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families