UniProtKB - P07567 (GAG_MPMV)
Protein
Gag polyprotein
Gene
gag
Organism
Mason-Pfizer monkey virus (MPMV) (Simian Mason-Pfizer virus)
Status
Functioni
Matrix protein.Curated
Nucleocapsid protein.Curated
capsid protein.Curated
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 547 – 564 | CCHC-type 1PROSITE-ProRule annotationAdd BLAST | 18 | |
Zinc fingeri | 576 – 593 | CCHC-type 2PROSITE-ProRule annotationAdd BLAST | 18 |
GO - Molecular functioni
- nucleic acid binding Source: InterPro
- structural constituent of virion Source: UniProtKB-KW
- zinc ion binding Source: InterPro
GO - Biological processi
- viral budding via host ESCRT complex Source: UniProtKB-KW
Keywordsi
Molecular function | Viral nucleoprotein |
Biological process | Host-virus interaction, Viral budding, Viral budding via the host ESCRT complexes, Viral release from host cell |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Gag polyproteinAlternative name(s): Core polyprotein Pr78 Cleaved into the following 7 chains: |
Gene namesi | Name:gag |
Organismi | Mason-Pfizer monkey virus (MPMV) (Simian Mason-Pfizer virus) |
Taxonomic identifieri | 11855 [NCBI] |
Taxonomic lineagei | Viruses › Riboviria › Pararnavirae › Artverviricota › Revtraviricetes › Ortervirales › Retroviridae › Orthoretrovirinae › Betaretrovirus |
Virus hosti | Macaca mulatta (Rhesus macaque) [TaxID: 9544] |
Proteomesi |
|
Subcellular locationi
- Virion Curated
- Virion Curated
- Virion Curated
Keywords - Cellular componenti
Capsid protein, Viral matrix protein, VirionPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 57 | R → F: Redirects assembly from host cytoplasm to plasma membrane. Loss of interaction with host DYNLT1. 1 Publication | 1 | |
Mutagenesisi | 203 – 205 | PPY → GAA: 80% loss of virus release. 1 Publication | 3 | |
Mutagenesisi | 210 – 211 | PS → AG: 30% loss of virus release. 1 Publication | 2 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed; by hostCurated | |||
ChainiPRO_0000443122 | 2 – 657 | Gag polyproteinAdd BLAST | 656 | |
ChainiPRO_0000040940 | 2 – 100 | Matrix protein p10Add BLAST | 99 | |
ChainiPRO_0000040941 | 101 – 216 | Phosphorylated protein pp24Add BLAST | 116 | |
PropeptideiPRO_0000443123 | 101 – 161 | CuratedAdd BLAST | 61 | |
ChainiPRO_0000443124 | 162 – 216 | Phosphorylated protein pp18Add BLAST | 55 | |
ChainiPRO_0000040942 | 217 – 299 | p12Add BLAST | 83 | |
ChainiPRO_0000040943 | 300 – 525 | Capsid protein p27Add BLAST | 226 | |
ChainiPRO_0000040944 | 526 – 621 | Nucleocapsid protein p14Add BLAST | 96 | |
ChainiPRO_0000040945 | 622 – 657 | p4Add BLAST | 36 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 2 | N-myristoyl glycine; by hostBy similarity | 1 |
Post-translational modificationi
Myristoylated. Myristoylation of the matrix (MA) domain mediates the transport and binding of Gag polyproteins to the host plasma membrane and is required for the assembly of viral particles.By similarity
Specific enzymatic cleavages in vivo yield mature proteins.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 100 – 101 | Cleavage; by viral protease1 Publication | 2 | |
Sitei | 162 – 163 | Cleavage; by viral protease1 Publication | 2 | |
Sitei | 216 – 217 | Cleavage; by viral protease1 Publication | 2 | |
Sitei | 299 – 300 | Cleavage; by viral protease1 Publication | 2 | |
Sitei | 525 – 526 | Cleavage; by viral protease1 Publication | 2 | |
Sitei | 621 – 622 | Cleavage; by viral protease1 Publication | 2 |
Keywords - PTMi
Lipoprotein, Myristate, PhosphoproteinInteractioni
Subunit structurei
Interacts with host DYNLT1; this interaction probably targets the viral polyproteins to the cytoplasmic assembly site.
1 PublicationBinary interactionsi
P07567
With | #Exp. | IntAct |
---|---|---|
DYNLT1 [P63172] from Homo sapiens. | 2 | EBI-15717123,EBI-1176455 |
Protein-protein interaction databases
DIPi | DIP-45130N |
ELMi | P07567 |
IntActi | P07567, 1 interactor |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P07567 |
SMRi | P07567 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P07567 |
Family & Domainsi
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 216 – 257 | Sequence analysisAdd BLAST | 42 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 202 – 205 | PPXY motif1 Publication | 4 | |
Motifi | 210 – 213 | PTAP/PSAP motif1 Publication | 4 |
Domaini
Late-budding domains (L domains) are short sequence motifs essential for viral particle release. They can occur individually or in close proximity within structural proteins. They interacts with sorting cellular proteins of the multivesicular body (MVB) pathway. Most of these proteins are class E vacuolar protein sorting factors belonging to ESCRT-I, ESCRT-II or ESCRT-III complexes. Phosphorylated protein pp24 and phosphorylated protein pp18 contains two L domains: a PTAP/PSAP motif which interacts with the UEV domain of TSG101, and a PPXY motif which binds to the WW domains of the ubiquitin ligase NEDD4. Both motifs contribute to viral release. The PSAP motif acts as an additional L domain and promotes the efficient release of the virions but requires an intact PPPY motif to perform its function.1 Publication
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 547 – 564 | CCHC-type 1PROSITE-ProRule annotationAdd BLAST | 18 | |
Zinc fingeri | 576 – 593 | CCHC-type 2PROSITE-ProRule annotationAdd BLAST | 18 |
Keywords - Domaini
Coiled coil, Repeat, Zinc-fingerFamily and domain databases
DisProti | DP01625 |
Gene3Di | 1.10.1200.30, 1 hit 1.10.150.490, 1 hit 1.10.375.10, 1 hit |
InterProi | View protein in InterPro IPR003322, B_retro_matrix IPR038124, B_retro_matrix_sf IPR000721, Gag_p24 IPR008916, Retrov_capsid_C IPR008919, Retrov_capsid_N IPR010999, Retrovr_matrix IPR001878, Znf_CCHC IPR036875, Znf_CCHC_sf |
Pfami | View protein in Pfam PF02337, Gag_p10, 1 hit PF00607, Gag_p24, 1 hit |
SMARTi | View protein in SMART SM00343, ZnF_C2HC, 2 hits |
SUPFAMi | SSF47836, SSF47836, 1 hit SSF47943, SSF47943, 1 hit SSF57756, SSF57756, 2 hits |
PROSITEi | View protein in PROSITE PS50158, ZF_CCHC, 1 hit |
s (3)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 3 produced by isoformsiribosomal frameshifting. AlignAdd to basketIsoform Gag polyprotein (identifier: P07567-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGQELSQHER YVEQLKQALK TRGVKVKYAD LLKFFDFVKD TCPWFPQEGT
60 70 80 90 100
IDIKRWRRVG DCFQDYYNTF GPEKVPVTAF SYWNLIKELI DKKEVNPQVM
110 120 130 140 150
AAVAQTEEIL KSNSQTDLTK TSQNPDLDLI SLDSDDEGAK SSSLQDKGLS
160 170 180 190 200
STKKPKRFPV LLTAQTSKDP EDPNPSEVDW DGLEDEAAKY HNPDWPPFLT
210 220 230 240 250
RPPPYNKATP SAPTVMAVVN PKEELKEKIA QLEEQIKLEE LHQALISKLQ
260 270 280 290 300
KLKTGNETVT HPDTAGGLSR TPHWPGQHIP KGKCCASREK EEQIPKDIFP
310 320 330 340 350
VTETVDGQGQ AWRHHNGFDF AVIKELKTAA SQYGATAPYT LAIVESVADN
360 370 380 390 400
WLTPTDWNTL VRAVLSGGDH LLWKSEFFEN CRDTAKRNQQ AGNGWDFDML
410 420 430 440 450
TGSGNYSSTD AQMQYDPGLF AQIQAAATKA WRKLPVKGDP GASLTGVKQG
460 470 480 490 500
PDEPFADFVH RLITTAGRIF GSAEAGVDYV KQLAYENANP ACQAAIRPYR
510 520 530 540 550
KKTDLTGYIR LCSDIGPSYQ QGLAMAAAFS GQTVKDFLNN KNKEKGGCCF
560 570 580 590 600
KCGKKGHFAK NCHEHAHNNA EPKVPGLCPR CKRGKHWANE CKSKTDNQGN
610 620 630 640 650
PIPPHQGNGW RGQPQAPKQA YGAVSFVPAN KNNPFQSLPE PPQEVQDWTS
VPPPTQY
Note: Produced by conventional translation.2 Publications
Isoform Gag-Pro polyprotein (identifier: P07570-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry P07570.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Isoform Gag-Pro-Pol polyprotein (identifier: P07572-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry P07572.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M12349 Genomic RNA Translation: AAA47710.1 AF033815 Genomic RNA Translation: AAC82573.1 |
PIRi | A25839, FOLJMP |
RefSeqi | NP_056893.1, NC_001550.1 [P07567-1] |
Genome annotation databases
GeneIDi | 2746974 |
KEGGi | vg:2746974 |
Keywords - Coding sequence diversityi
Ribosomal frameshiftingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M12349 Genomic RNA Translation: AAA47710.1 AF033815 Genomic RNA Translation: AAC82573.1 |
PIRi | A25839, FOLJMP |
RefSeqi | NP_056893.1, NC_001550.1 [P07567-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1BAX | NMR | - | A | 1-94 | [»] | |
1CL4 | NMR | - | A | 546-605 | [»] | |
2F76 | NMR | - | X | 2-100 | [»] | |
2F77 | NMR | - | X | 1-100 | [»] | |
2KGF | NMR | - | A | 300-439 | [»] | |
2LPY | NMR | - | A | 2-118 | [»] | |
2MV4 | NMR | - | A | 2-118 | [»] | |
4ARD | electron microscopy | 7.00 | A/B | 318-433 | [»] | |
5LDL | NMR | - | A | 2-118 | [»] | |
5LMY | NMR | - | A | 2-118 | [»] | |
BMRBi | P07567 | |||||
SMRi | P07567 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
DIPi | DIP-45130N |
ELMi | P07567 |
IntActi | P07567, 1 interactor |
Genome annotation databases
GeneIDi | 2746974 |
KEGGi | vg:2746974 |
Miscellaneous databases
EvolutionaryTracei | P07567 |
Family and domain databases
DisProti | DP01625 |
Gene3Di | 1.10.1200.30, 1 hit 1.10.150.490, 1 hit 1.10.375.10, 1 hit |
InterProi | View protein in InterPro IPR003322, B_retro_matrix IPR038124, B_retro_matrix_sf IPR000721, Gag_p24 IPR008916, Retrov_capsid_C IPR008919, Retrov_capsid_N IPR010999, Retrovr_matrix IPR001878, Znf_CCHC IPR036875, Znf_CCHC_sf |
Pfami | View protein in Pfam PF02337, Gag_p10, 1 hit PF00607, Gag_p24, 1 hit |
SMARTi | View protein in SMART SM00343, ZnF_C2HC, 2 hits |
SUPFAMi | SSF47836, SSF47836, 1 hit SSF47943, SSF47943, 1 hit SSF57756, SSF57756, 2 hits |
PROSITEi | View protein in PROSITE PS50158, ZF_CCHC, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GAG_MPMV | |
Accessioni | P07567Primary (citable) accession number: P07567 Secondary accession number(s): O56225 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1988 |
Last sequence update: | January 23, 2007 | |
Last modified: | April 7, 2021 | |
This is version 146 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencingDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references