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Protein

Gag polyprotein

Gene

gag

Organism
Mason-Pfizer monkey virus (MPMV) (Simian Mason-Pfizer virus)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Matrix protein p10: Matrix protein.Curated
Nucleocapsid protein p14: Nucleocapsid protein.Curated
Capsid protein p27: capsid protein.Curated

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri547 – 564CCHC-type 1PROSITE-ProRule annotationAdd BLAST18
Zinc fingeri576 – 593CCHC-type 2PROSITE-ProRule annotationAdd BLAST18

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionViral nucleoprotein
Biological processHost-virus interaction, Viral budding, Viral budding via the host ESCRT complexes, Viral release from host cell
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Gag polyprotein
Alternative name(s):
Core polyprotein
Pr78
Cleaved into the following 7 chains:
Gene namesi
Name:gag
OrganismiMason-Pfizer monkey virus (MPMV) (Simian Mason-Pfizer virus)
Taxonomic identifieri11855 [NCBI]
Taxonomic lineageiVirusesOrterviralesRetroviridaeOrthoretrovirinaeBetaretrovirus
Virus hostiMacaca mulatta (Rhesus macaque) [TaxID: 9544]
Proteomesi
  • UP000008870 Componenti: Genome
  • UP000105838 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

  • host cell cytoplasm Source: UniProtKB
  • viral nucleocapsid Source: UniProtKB-KW

Keywords - Cellular componenti

Capsid protein, Viral matrix protein, Virion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi57R → F: Redirects assembly from host cytoplasm to plasma membrane. Loss of interaction with host DYNLT1. 1 Publication1
Mutagenesisi203 – 205PPY → GAA: 80% loss of virus release. 1 Publication3
Mutagenesisi210 – 211PS → AG: 30% loss of virus release. 1 Publication2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved; by hostCurated
ChainiPRO_00004431222 – 657Gag polyproteinAdd BLAST656
ChainiPRO_00000409402 – 100Matrix protein p10Add BLAST99
ChainiPRO_0000040941101 – 216Phosphorylated protein pp24Add BLAST116
PropeptideiPRO_0000443123101 – 161CuratedAdd BLAST61
ChainiPRO_0000443124162 – 216Phosphorylated protein pp18Add BLAST55
ChainiPRO_0000040942217 – 299p12Add BLAST83
ChainiPRO_0000040943300 – 525Capsid protein p27Add BLAST226
ChainiPRO_0000040944526 – 621Nucleocapsid protein p14Add BLAST96
ChainiPRO_0000040945622 – 657p4Add BLAST36

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine; by hostBy similarity1

Post-translational modificationi

Gag polyprotein: Myristoylated. Myristoylation of the matrix (MA) domain mediates the transport and binding of Gag polyproteins to the host plasma membrane and is required for the assembly of viral particles.By similarity
Gag polyprotein: Specific enzymatic cleavages in vivo yield mature proteins.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei100 – 101Cleavage; by viral protease1 Publication2
Sitei162 – 163Cleavage; by viral protease1 Publication2
Sitei216 – 217Cleavage; by viral protease1 Publication2
Sitei299 – 300Cleavage; by viral protease1 Publication2
Sitei525 – 526Cleavage; by viral protease1 Publication2
Sitei621 – 622Cleavage; by viral protease1 Publication2

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Miscellaneous databases

PMAP-CutDBiP07567

Interactioni

Subunit structurei

Gag polyprotein: Interacts with host DYNLT1; this interaction probably targets the viral polyproteins to the cytoplasmic assembly site.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
DYNLT1P631722EBI-15717123,EBI-1176455From Homo sapiens.

Protein-protein interaction databases

DIPiDIP-45130N
ELMiP07567
IntActiP07567, 1 interactor

Structurei

Secondary structure

1657
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 5Combined sources3
Helixi7 – 20Combined sources14
Turni21 – 23Combined sources3
Helixi28 – 41Combined sources14
Turni46 – 48Combined sources3
Helixi52 – 70Combined sources19
Turni72 – 74Combined sources3
Helixi78 – 93Combined sources16
Helixi98 – 101Combined sources4
Helixi103 – 106Combined sources4
Beta strandi112 – 115Combined sources4
Beta strandi301 – 305Combined sources5
Beta strandi311 – 315Combined sources5
Helixi320 – 332Combined sources13
Helixi338 – 348Combined sources11
Helixi354 – 364Combined sources11
Helixi368 – 391Combined sources24
Beta strandi396 – 398Combined sources3
Turni399 – 401Combined sources3
Beta strandi404 – 406Combined sources3
Helixi408 – 414Combined sources7
Helixi417 – 430Combined sources14
Beta strandi596 – 599Combined sources4

3D structure databases

ProteinModelPortaliP07567
SMRiP07567
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07567

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili216 – 257Sequence analysisAdd BLAST42

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi202 – 205PPXY motif1 Publication4
Motifi210 – 213PTAP/PSAP motif1 Publication4

Domaini

Gag polyprotein: Late-budding domains (L domains) are short sequence motifs essential for viral particle release. They can occur individually or in close proximity within structural proteins. They interacts with sorting cellular proteins of the multivesicular body (MVB) pathway. Most of these proteins are class E vacuolar protein sorting factors belonging to ESCRT-I, ESCRT-II or ESCRT-III complexes. Phosphorylated protein pp24 and phosphorylated protein pp18 contains two L domains: a PTAP/PSAP motif which interacts with the UEV domain of TSG101, and a PPXY motif which binds to the WW domains of the ubiquitin ligase NEDD4. Both motifs contribute to viral release. The PSAP motif acts as an additional L domain and promotes the efficient release of the virions but requires an intact PPPY motif to perform its function.1 Publication

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri547 – 564CCHC-type 1PROSITE-ProRule annotationAdd BLAST18
Zinc fingeri576 – 593CCHC-type 2PROSITE-ProRule annotationAdd BLAST18

Keywords - Domaini

Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

OrthoDBiVOG090001BU

Family and domain databases

Gene3Di1.10.1200.30, 1 hit
1.10.150.490, 1 hit
1.10.375.10, 1 hit
InterProiView protein in InterPro
IPR003322 B_retro_matrix
IPR038124 B_retro_matrix_sf
IPR000721 Gag_p24
IPR008916 Retrov_capsid_C
IPR008919 Retrov_capsid_N
IPR010999 Retrovr_matrix
IPR001878 Znf_CCHC
IPR036875 Znf_CCHC_sf
PfamiView protein in Pfam
PF02337 Gag_p10, 1 hit
PF00607 Gag_p24, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD004265 B_retro_matrix_N, 1 hit
SMARTiView protein in SMART
SM00343 ZnF_C2HC, 2 hits
SUPFAMiSSF47836 SSF47836, 1 hit
SSF47943 SSF47943, 1 hit
SSF57756 SSF57756, 2 hits
PROSITEiView protein in PROSITE
PS50158 ZF_CCHC, 1 hit

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by ribosomal frameshifting. AlignAdd to basket

Isoform Gag polyprotein (identifier: P07567-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGQELSQHER YVEQLKQALK TRGVKVKYAD LLKFFDFVKD TCPWFPQEGT
60 70 80 90 100
IDIKRWRRVG DCFQDYYNTF GPEKVPVTAF SYWNLIKELI DKKEVNPQVM
110 120 130 140 150
AAVAQTEEIL KSNSQTDLTK TSQNPDLDLI SLDSDDEGAK SSSLQDKGLS
160 170 180 190 200
STKKPKRFPV LLTAQTSKDP EDPNPSEVDW DGLEDEAAKY HNPDWPPFLT
210 220 230 240 250
RPPPYNKATP SAPTVMAVVN PKEELKEKIA QLEEQIKLEE LHQALISKLQ
260 270 280 290 300
KLKTGNETVT HPDTAGGLSR TPHWPGQHIP KGKCCASREK EEQIPKDIFP
310 320 330 340 350
VTETVDGQGQ AWRHHNGFDF AVIKELKTAA SQYGATAPYT LAIVESVADN
360 370 380 390 400
WLTPTDWNTL VRAVLSGGDH LLWKSEFFEN CRDTAKRNQQ AGNGWDFDML
410 420 430 440 450
TGSGNYSSTD AQMQYDPGLF AQIQAAATKA WRKLPVKGDP GASLTGVKQG
460 470 480 490 500
PDEPFADFVH RLITTAGRIF GSAEAGVDYV KQLAYENANP ACQAAIRPYR
510 520 530 540 550
KKTDLTGYIR LCSDIGPSYQ QGLAMAAAFS GQTVKDFLNN KNKEKGGCCF
560 570 580 590 600
KCGKKGHFAK NCHEHAHNNA EPKVPGLCPR CKRGKHWANE CKSKTDNQGN
610 620 630 640 650
PIPPHQGNGW RGQPQAPKQA YGAVSFVPAN KNNPFQSLPE PPQEVQDWTS

VPPPTQY
Note: Produced by conventional translation.2 Publications
Length:657
Mass (Da):73,110
Last modified:January 23, 2007 - v3
Checksum:i301888B033BC3061
GO
Isoform Gag-Pro polyprotein (identifier: P07570-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry P07570.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Produced by -1 ribosomal frameshifting between gag-pro.2 Publications
Length:911
Mass (Da):100,648
GO
Isoform Gag-Pro-Pol polyprotein (identifier: P07572-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry P07572.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Produced by -1 ribosomal frameshiftings between gag-pro and pro-pol.2 Publications
Length:1,771
Mass (Da):198,014
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12349 Genomic RNA Translation: AAA47710.1
AF033815 Genomic RNA Translation: AAC82573.1
PIRiA25839 FOLJMP
RefSeqiNP_056893.1, NC_001550.1 [P07567-1]

Genome annotation databases

GeneIDi2746974
KEGGivg:2746974

Keywords - Coding sequence diversityi

Ribosomal frameshifting

Similar proteinsi

Entry informationi

Entry nameiGAG_MPMV
AccessioniPrimary (citable) accession number: P07567
Secondary accession number(s): O56225
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: January 23, 2007
Last modified: June 20, 2018
This is version 136 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

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