UniProtKB - P07567 (GAG_MPMV)

BLAST

>sp|P07567|GAG_MPMV Gag polyprotein OS=Mason-Pfizer monkey virus OX=11855 GN=gag PE=1 SV=3
MGQELSQHERYVEQLKQALKTRGVKVKYADLLKFFDFVKDTCPWFPQEGTIDIKRWRRVG
DCFQDYYNTFGPEKVPVTAFSYWNLIKELIDKKEVNPQVMAAVAQTEEILKSNSQTDLTK
TSQNPDLDLISLDSDDEGAKSSSLQDKGLSSTKKPKRFPVLLTAQTSKDPEDPNPSEVDW
DGLEDEAAKYHNPDWPPFLTRPPPYNKATPSAPTVMAVVNPKEELKEKIAQLEEQIKLEE
LHQALISKLQKLKTGNETVTHPDTAGGLSRTPHWPGQHIPKGKCCASREKEEQIPKDIFP
VTETVDGQGQAWRHHNGFDFAVIKELKTAASQYGATAPYTLAIVESVADNWLTPTDWNTL
VRAVLSGGDHLLWKSEFFENCRDTAKRNQQAGNGWDFDMLTGSGNYSSTDAQMQYDPGLF
AQIQAAATKAWRKLPVKGDPGASLTGVKQGPDEPFADFVHRLITTAGRIFGSAEAGVDYV
KQLAYENANPACQAAIRPYRKKTDLTGYIRLCSDIGPSYQQGLAMAAAFSGQTVKDFLNN
KNKEKGGCCFKCGKKGHFAKNCHEHAHNNAEPKVPGLCPRCKRGKHWANECKSKTDNQGN
PIPPHQGNGWRGQPQAPKQAYGAVSFVPANKNNPFQSLPEPPQEVQDWTSVPPPTQY

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History
Entry version 136 (20 Jun 2018)
Sequence version 3 (23 Jan 2007)
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Protein

Gag polyprotein

Gene

gag

Organism

Mason-Pfizer monkey virus (MPMV) (Simian Mason-Pfizer virus)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

Matrix protein p10: Matrix protein.Curated

Nucleocapsid protein p14: Nucleocapsid protein.Curated

Capsid protein p27: capsid protein.Curated

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Zinc fingerⁱ	547 – 564	CCHC-type 1PROSITE-ProRule annotationAdd BLAST		18
Zinc fingerⁱ	576 – 593	CCHC-type 2PROSITE-ProRule annotationAdd BLAST		18

GO - Molecular functionⁱ

nucleic acid binding Source: InterPro
structural constituent of virion Source: UniProtKB-KW
zinc ion binding Source: InterPro

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

viral budding via host ESCRT complex Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Viral nucleoprotein
Biological process	Host-virus interaction, Viral budding, Viral budding via the host ESCRT complexes, Viral release from host cell
Ligand	Metal-binding, Zinc

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Gag polyprotein Alternative name(s): Core polyprotein Pr78 Cleaved into the following 7 chains: Matrix protein p10 Phosphorylated protein pp24 Phosphorylated protein pp18 p12 Capsid protein p27 Nucleocapsid protein p14 p4
Gene namesⁱ	Name:gag
Organismⁱ	Mason-Pfizer monkey virus (MPMV) (Simian Mason-Pfizer virus)
Taxonomic identifierⁱ	11855 [NCBI]
Taxonomic lineageⁱ	Viruses › Ortervirales › Retroviridae › Orthoretrovirinae › Betaretrovirus
Virus hostⁱ	Macaca mulatta (Rhesus macaque) [TaxID: 9544]
Proteomesⁱ	UP000008870 Componentⁱ: Genome UP000105838 Componentⁱ: Genome

Subcellular locationⁱ

Matrix protein p10 :

Virion Curated

Capsid protein p27 :

Virion Curated

Nucleocapsid protein p14 :

Virion Curated

GO - Cellular componentⁱ

host cell cytoplasm
Source: UniProtKB
Inferred from direct assayⁱ
- 18647839
viral nucleocapsid Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Capsid protein, Viral matrix protein, Virion

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	57	R → F: Redirects assembly from host cytoplasm to plasma membrane. Loss of interaction with host DYNLT1. 1 Publication	1
Mutagenesisⁱ	203 – 205	PPY → GAA: 80% loss of virus release. 1 Publication	3
Mutagenesisⁱ	210 – 211	PS → AG: 30% loss of virus release. 1 Publication	2

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Length
Initiator methionineⁱ		Removed; by hostCurated
ChainⁱPRO_0000443122	2 – 657	Gag polyproteinAdd BLAST	656
ChainⁱPRO_0000040940	2 – 100	Matrix protein p10Add BLAST	99
ChainⁱPRO_0000040941	101 – 216	Phosphorylated protein pp24Add BLAST	116
PropeptideⁱPRO_0000443123	101 – 161	CuratedAdd BLAST	61
ChainⁱPRO_0000443124	162 – 216	Phosphorylated protein pp18Add BLAST	55
ChainⁱPRO_0000040942	217 – 299	p12Add BLAST	83
ChainⁱPRO_0000040943	300 – 525	Capsid protein p27Add BLAST	226
ChainⁱPRO_0000040944	526 – 621	Nucleocapsid protein p14Add BLAST	96
ChainⁱPRO_0000040945	622 – 657	p4Add BLAST	36

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Lipidationⁱ	2	N-myristoyl glycine; by hostBy similarity		1

Post-translational modificationⁱ

Gag polyprotein: Myristoylated. Myristoylation of the matrix (MA) domain mediates the transport and binding of Gag polyproteins to the host plasma membrane and is required for the assembly of viral particles.By similarity

Gag polyprotein: Specific enzymatic cleavages in vivo yield mature proteins.1 Publication

Sites

Feature key	Position(s)	DescriptionActions	Length
Siteⁱ	100 – 101	Cleavage; by viral protease1 Publication	2
Siteⁱ	162 – 163	Cleavage; by viral protease1 Publication	2
Siteⁱ	216 – 217	Cleavage; by viral protease1 Publication	2
Siteⁱ	299 – 300	Cleavage; by viral protease1 Publication	2
Siteⁱ	525 – 526	Cleavage; by viral protease1 Publication	2
Siteⁱ	621 – 622	Cleavage; by viral protease1 Publication	2

Keywords - PTMⁱ

Lipoprotein, Myristate, Phosphoprotein

Miscellaneous databases

PMAP-CutDBⁱ	P07567

PMAP-CutDBⁱ

P07567

Interactionⁱ

Subunit structureⁱ

Gag polyprotein: Interacts with host DYNLT1; this interaction probably targets the viral polyproteins to the cytoplasmic assembly site.1 Publication

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
DYNLT1	P63172	2	EBI-15717123,EBI-1176455	From Homo sapiens.

With

Entry

#Exp.

IntAct

Notes

DYNLT1

P63172

EBI-15717123,EBI-1176455

From Homo sapiens.

Protein-protein interaction databases

Database of interacting proteins More...DIPⁱ	DIP-45130N
ELMⁱ	P07567
IntActⁱ	P07567, 1 interactor

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	3 – 5	Combined sources	3
Helixⁱ	7 – 20	Combined sources	14
Turnⁱ	21 – 23	Combined sources	3
Helixⁱ	28 – 41	Combined sources	14
Turnⁱ	46 – 48	Combined sources	3
Helixⁱ	52 – 70	Combined sources	19
Turnⁱ	72 – 74	Combined sources	3
Helixⁱ	78 – 93	Combined sources	16
Helixⁱ	98 – 101	Combined sources	4
Helixⁱ	103 – 106	Combined sources	4
Beta strandⁱ	112 – 115	Combined sources	4
Beta strandⁱ	301 – 305	Combined sources	5
Beta strandⁱ	311 – 315	Combined sources	5
Helixⁱ	320 – 332	Combined sources	13
Helixⁱ	338 – 348	Combined sources	11
Helixⁱ	354 – 364	Combined sources	11
Helixⁱ	368 – 391	Combined sources	24
Beta strandⁱ	396 – 398	Combined sources	3
Turnⁱ	399 – 401	Combined sources	3
Beta strandⁱ	404 – 406	Combined sources	3
Helixⁱ	408 – 414	Combined sources	7
Helixⁱ	417 – 430	Combined sources	14
Beta strandⁱ	596 – 599	Combined sources	4

Show more details

3D structure databases

ProteinModelPortalⁱ	P07567
SMRⁱ	P07567
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ

P07567

Family & Domainsⁱ

Coiled coil

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Coiled coilⁱ	216 – 257	Sequence analysisAdd BLAST		42

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	202 – 205	PPXY motif1 Publication		4
Motifⁱ	210 – 213	PTAP/PSAP motif1 Publication		4

Domainⁱ

Gag polyprotein: Late-budding domains (L domains) are short sequence motifs essential for viral particle release. They can occur individually or in close proximity within structural proteins. They interacts with sorting cellular proteins of the multivesicular body (MVB) pathway. Most of these proteins are class E vacuolar protein sorting factors belonging to ESCRT-I, ESCRT-II or ESCRT-III complexes. Phosphorylated protein pp24 and phosphorylated protein pp18 contains two L domains: a PTAP/PSAP motif which interacts with the UEV domain of TSG101, and a PPXY motif which binds to the WW domains of the ubiquitin ligase NEDD4. Both motifs contribute to viral release. The PSAP motif acts as an additional L domain and promotes the efficient release of the virions but requires an intact PPPY motif to perform its function.1 Publication

Zinc finger

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Zinc fingerⁱ	547 – 564	CCHC-type 1PROSITE-ProRule annotationAdd BLAST		18
Zinc fingerⁱ	576 – 593	CCHC-type 2PROSITE-ProRule annotationAdd BLAST		18

Keywords - Domainⁱ

Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

Database of Orthologous Groups More...OrthoDBⁱ	VOG090001BU

OrthoDBⁱ

VOG090001BU

Family and domain databases

Gene3Dⁱ	1.10.1200.30, 1 hit 1.10.150.490, 1 hit 1.10.375.10, 1 hit
InterProⁱ	View protein in InterPro IPR003322 B_retro_matrix IPR038124 B_retro_matrix_sf IPR000721 Gag_p24 IPR008916 Retrov_capsid_C IPR008919 Retrov_capsid_N IPR010999 Retrovr_matrix IPR001878 Znf_CCHC IPR036875 Znf_CCHC_sf
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF02337 Gag_p10, 1 hit PF00607 Gag_p24, 1 hit
ProDomⁱ	View protein in ProDom or Entries sharing at least one domain PD004265 B_retro_matrix_N, 1 hit
SMARTⁱ	View protein in SMART SM00343 ZnF_C2HC, 2 hits
SUPFAMⁱ	SSF47836 SSF47836, 1 hit SSF47943 SSF47943, 1 hit SSF57756 SSF57756, 2 hits
PROSITEⁱ	View protein in PROSITE PS50158 ZF_CCHC, 1 hit

Sequences (3)ⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsⁱ produced by ribosomal frameshifting. Align Add to basket

Isoform Gag polyprotein (identifier: P07567-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGQELSQHER YVEQLKQALK TRGVKVKYAD LLKFFDFVKD TCPWFPQEGT 
        60         70         80         90        100
IDIKRWRRVG DCFQDYYNTF GPEKVPVTAF SYWNLIKELI DKKEVNPQVM 
       110        120        130        140        150
AAVAQTEEIL KSNSQTDLTK TSQNPDLDLI SLDSDDEGAK SSSLQDKGLS 
       160        170        180        190        200
STKKPKRFPV LLTAQTSKDP EDPNPSEVDW DGLEDEAAKY HNPDWPPFLT 
       210        220        230        240        250
RPPPYNKATP SAPTVMAVVN PKEELKEKIA QLEEQIKLEE LHQALISKLQ 
       260        270        280        290        300
KLKTGNETVT HPDTAGGLSR TPHWPGQHIP KGKCCASREK EEQIPKDIFP 
       310        320        330        340        350
VTETVDGQGQ AWRHHNGFDF AVIKELKTAA SQYGATAPYT LAIVESVADN 
       360        370        380        390        400
WLTPTDWNTL VRAVLSGGDH LLWKSEFFEN CRDTAKRNQQ AGNGWDFDML 
       410        420        430        440        450
TGSGNYSSTD AQMQYDPGLF AQIQAAATKA WRKLPVKGDP GASLTGVKQG 
       460        470        480        490        500
PDEPFADFVH RLITTAGRIF GSAEAGVDYV KQLAYENANP ACQAAIRPYR 
       510        520        530        540        550
KKTDLTGYIR LCSDIGPSYQ QGLAMAAAFS GQTVKDFLNN KNKEKGGCCF 
       560        570        580        590        600
KCGKKGHFAK NCHEHAHNNA EPKVPGLCPR CKRGKHWANE CKSKTDNQGN 
       610        620        630        640        650
PIPPHQGNGW RGQPQAPKQA YGAVSFVPAN KNNPFQSLPE PPQEVQDWTS 

VPPPTQY

Note: Produced by conventional translation.2 Publications

Length:657

Mass (Da):73,110

Last modified:January 23, 2007 - v3

Checksum:ⁱ301888B033BC3061

Isoform Gag-Pro polyprotein (identifier: P07570-1) [UniParc]FASTA Add to basket

The sequence of this isoform can be found in the external entry P07570.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: Produced by -1 ribosomal frameshifting between gag-pro.2 Publications

Length:911

Mass (Da):100,648

Isoform Gag-Pro-Pol polyprotein (identifier: P07572-1) [UniParc]FASTA Add to basket

The sequence of this isoform can be found in the external entry P07572.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: Produced by -1 ribosomal frameshiftings between gag-pro and pro-pol.2 Publications

Length:1,771

Mass (Da):198,014

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M12349 Genomic RNA Translation: AAA47710.1 AF033815 Genomic RNA Translation: AAC82573.1
PIRⁱ	A25839 FOLJMP
NCBI Reference Sequences More...RefSeqⁱ	NP_056893.1, NC_001550.1 [P07567-1]

Genome annotation databases

GeneIDⁱ	2746974
KEGGⁱ	vg:2746974

Keywords - Coding sequence diversityⁱ

Ribosomal frameshifting

Protein	Similar proteins		Species	Score	Length	Source
P07567	Gag polyprotein	)	SRV1		658	UniRef90_P07567
UPI0000231CCB		MPMV		97

Protein	Similar proteins		Species	Score	Length	Source
P07567	Gag polyprotein	)	SRV1		658	UniRef50_P07567
Gag polyprotein		SRV2		654
Gag protein		CHLSB		658
Gag polyprotein		Simian endogenous retrovirus vero ATCC CCL-81		658
Gag polyprotein		Simian endogenous retrovirus		658
+17

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M12349 Genomic RNA Translation: AAA47710.1 AF033815 Genomic RNA Translation: AAC82573.1
PIRⁱ	A25839 FOLJMP
RefSeqⁱ	NP_056893.1, NC_001550.1 [P07567-1]

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1BAX	NMR	-	A	1-94	[»]
	1CL4	NMR	-	A	546-605	[»]
	2F76	NMR	-	X	2-100	[»]
	2F77	NMR	-	X	1-100	[»]
	2KGF	NMR	-	A	300-439	[»]
	2LPY	NMR	-	A	2-118	[»]
	2MV4	NMR	-	A	2-118	[»]
	4ARD	electron microscopy	7.00	A/B	318-433	[»]
	5LDL	NMR	-	A	2-118	[»]
	5LMY	NMR	-	A	2-118	[»]
ProteinModelPortalⁱ	P07567
SMRⁱ	P07567
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

DIPⁱ	DIP-45130N
ELMⁱ	P07567
IntActⁱ	P07567, 1 interactor

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

GeneIDⁱ	2746974
KEGGⁱ	vg:2746974

Phylogenomic databases

OrthoDBⁱ	VOG090001BU

OrthoDBⁱ

VOG090001BU

Miscellaneous databases

EvolutionaryTraceⁱ	P07567
PMAP-CutDBⁱ	P07567

Family and domain databases

Gene3Dⁱ	1.10.1200.30, 1 hit 1.10.150.490, 1 hit 1.10.375.10, 1 hit
InterProⁱ	View protein in InterPro IPR003322 B_retro_matrix IPR038124 B_retro_matrix_sf IPR000721 Gag_p24 IPR008916 Retrov_capsid_C IPR008919 Retrov_capsid_N IPR010999 Retrovr_matrix IPR001878 Znf_CCHC IPR036875 Znf_CCHC_sf
Pfamⁱ	View protein in Pfam PF02337 Gag_p10, 1 hit PF00607 Gag_p24, 1 hit
ProDomⁱ	View protein in ProDom or Entries sharing at least one domain PD004265 B_retro_matrix_N, 1 hit
SMARTⁱ	View protein in SMART SM00343 ZnF_C2HC, 2 hits
SUPFAMⁱ	SSF47836 SSF47836, 1 hit SSF47943 SSF47943, 1 hit SSF57756 SSF57756, 2 hits
PROSITEⁱ	View protein in PROSITE PS50158 ZF_CCHC, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	GAG_MPMV
Accessionⁱ	P07567Primary (citable) accession number: P07567 Secondary accession number(s): O56225
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
	Last sequence update:	January 23, 2007
	Last modified:	June 20, 2018
	This is version 136 of the entry and version 3 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Viral Protein Annotation Program

UniProtKB

UniParc

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Proteomes

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Supporting data

UniProtKB - P07567 (GAG_MPMV)

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Gag polyprotein

gag

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Sites

Keywords - PTMi

Miscellaneous databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Coiled coil

Motif

Zinc finger

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Keywords - Domainⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ