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Protein

Mitosis inhibitor protein kinase wee1

Gene

wee1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Protein kinase that acts both on serines and on tyrosines. It acts as a dosage-dependent negative regulator of entry into mitosis (G2 to M transition). Phosphorylates and inhibits cdc2.1 Publication

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit.By similarity

Activity regulationi

Negatively regulated by phosphorylation in the M-phase.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei596ATPPROSITE-ProRule annotation1
Active sitei693Proton acceptorPROSITE-ProRule annotation1
Metal bindingi698Magnesium; via carbonyl oxygenBy similarity1
Metal bindingi711Magnesium; via carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi572 – 580ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase, Tyrosine-protein kinase
Biological processCell cycle, Cell division, Mitosis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-SPO-156711 Polo-like kinase mediated events
R-SPO-69273 Cyclin A/B1/B2 associated events during G2/M transition
R-SPO-69478 G2/M DNA replication checkpoint

Names & Taxonomyi

Protein namesi
Recommended name:
Mitosis inhibitor protein kinase wee1 (EC:2.7.10.2)
Alternative name(s):
P107 protein kinase homolog
Gene namesi
Name:wee1
ORF Names:SPCC18B5.03
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC18B5.03
PomBaseiSPCC18B5.03 wee1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi596K → L: Inactivates enzyme. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000868151 – 877Mitosis inhibitor protein kinase wee1Add BLAST877

Post-translational modificationi

Phosphorylated in the C-terminal by NIM1/CDR1.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP07527
PRIDEiP07527

PTM databases

iPTMnetiP07527
SwissPalmiP07527

Interactioni

Protein-protein interaction databases

BioGridi275695, 80 interactors
DIPiDIP-16N
STRINGi4896.SPCC18B5.03.1

Structurei

3D structure databases

ProteinModelPortaliP07527
SMRiP07527
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini566 – 843Protein kinasePROSITE-ProRule annotationAdd BLAST278

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.PROSITE-ProRule annotation

Phylogenomic databases

InParanoidiP07527
KOiK03114
OMAiKIKHASP
OrthoDBiEOG092C38EF

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

P07527-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSSSSNTSSH RSYGLRRSQR SMNLNRATLL APPTPSSLYD ANNSTSSTSS
60 70 80 90 100
QKPNTSFTSL FGPRKQTTSS PSFSHAAPLH PLSPPSFTHS QPQIQAQPVP
110 120 130 140 150
RRPSLFDRPN LVSRSSSRLG DSPSLSPVAQ VANPIHHTAP SPSDVRAFPI
160 170 180 190 200
HKNASTGVKR SFFSSSMSNG AMSPPSHSPS PFLQSSQHIP PSTPAQKLRK
210 220 230 240 250
KNNFDSFRIS NSHISPFASG SFSPFATSSP NFLSTSTPAP PNSNNANPST
260 270 280 290 300
LFSSIPSSRH TTSNHFPSNS AQSSLFSPTA RPLTARKLGF ASSQTKSAVS
310 320 330 340 350
NNHSRNSSKD ASFMMKSFIP SNRSHPQTQQ NESSLFSDNS MVNSSSNSFS
360 370 380 390 400
LFPNATLPNP PSSELLTTPF QQIKPPSQVF MSTGLLSKQH RPRKNINFTP
410 420 430 440 450
LPPSTPSKPS TFVRPHSSST DSPPSPSTPS NTQTDSYFIQ RENTPTNHNS
460 470 480 490 500
IPTIQLEKSS MDFLRFDPPP SAVKTSHNYG LPFLSNQRCP ATPTRNPFAF
510 520 530 540 550
ENTVSIHMDG RQPSPIKSRN NNQMSFAMEE EADVSQPSSS SFTLSFPSAL
560 570 580 590 600
TSSKVSSSTS HLLTRFRNVT LLGSGEFSEV FQVEDPVEKT LKYAVKKLKV
610 620 630 640 650
KFSGPKERNR LLQEVSIQRA LKGHDHIVEL MDSWEHGGFL YMQVELCENG
660 670 680 690 700
SLDRFLEEQG QLSRLDEFRV WKILVEVALG LQFIHHKNYV HLDLKPANVM
710 720 730 740 750
ITFEGTLKIG DFGMASVWPV PRGMEREGDC EYIAPEVLAN HLYDKPADIF
760 770 780 790 800
SLGITVFEAA ANIVLPDNGQ SWQKLRSGDL SDAPRLSSTD NGSSLTSSSR
810 820 830 840 850
ETPANSIIGQ GGLDRVVEWM LSPEPRNRPT IDQILATDEV CWVEMRRKAG
860 870
AIIYEGIHGS SSNPQGDQMM EDWQVNV
Length:877
Mass (Da):96,261
Last modified:April 1, 1988 - v1
Checksum:iC8B5113D66C39D10
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16508 Genomic DNA Translation: AAA35354.1
CU329672 Genomic DNA Translation: CAB52150.1
AB027900 Genomic DNA Translation: BAA87204.1
PIRiA25962
RefSeqiNP_587933.1, NM_001022924.2

Genome annotation databases

EnsemblFungiiSPCC18B5.03.1; SPCC18B5.03.1:pep; SPCC18B5.03
GeneIDi2539123
KEGGispo:SPCC18B5.03

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16508 Genomic DNA Translation: AAA35354.1
CU329672 Genomic DNA Translation: CAB52150.1
AB027900 Genomic DNA Translation: BAA87204.1
PIRiA25962
RefSeqiNP_587933.1, NM_001022924.2

3D structure databases

ProteinModelPortaliP07527
SMRiP07527
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi275695, 80 interactors
DIPiDIP-16N
STRINGi4896.SPCC18B5.03.1

PTM databases

iPTMnetiP07527
SwissPalmiP07527

Proteomic databases

PaxDbiP07527
PRIDEiP07527

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPCC18B5.03.1; SPCC18B5.03.1:pep; SPCC18B5.03
GeneIDi2539123
KEGGispo:SPCC18B5.03

Organism-specific databases

EuPathDBiFungiDB:SPCC18B5.03
PomBaseiSPCC18B5.03 wee1

Phylogenomic databases

InParanoidiP07527
KOiK03114
OMAiKIKHASP
OrthoDBiEOG092C38EF

Enzyme and pathway databases

ReactomeiR-SPO-156711 Polo-like kinase mediated events
R-SPO-69273 Cyclin A/B1/B2 associated events during G2/M transition
R-SPO-69478 G2/M DNA replication checkpoint

Miscellaneous databases

PROiPR:P07527

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiWEE1_SCHPO
AccessioniPrimary (citable) accession number: P07527
Secondary accession number(s): Q9UU00
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: November 7, 2018
This is version 173 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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