We will be switching to the new UniProt website soon. Please explore and share your feedback.
Take me to the new website.
UniProtKB - P07478 (TRY2_HUMAN)
Protein
Trypsin-2
Gene
PRSS2
Organism
Homo sapiens (Human)
Status
Functioni
In the ileum, may be involved in defensin processing, including DEFA5.
1 PublicationCatalytic activityi
- Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa. EC:3.4.21.4
Cofactori
Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 63 | Charge relay systemBy similarity | 1 | |
Metal bindingi | 75 | CalciumBy similarity | 1 | |
Metal bindingi | 77 | Calcium; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 80 | Calcium; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 85 | CalciumBy similarity | 1 | |
Active sitei | 107 | Charge relay systemBy similarity | 1 | |
Sitei | 194 | Required for specificityBy similarity | 1 | |
Active sitei | 200 | Charge relay systemBy similarity | 1 |
GO - Molecular functioni
- calcium ion binding Source: UniProtKB
- metalloendopeptidase activity Source: Reactome
- serine-type endopeptidase activity Source: UniProtKB
- serine-type peptidase activity Source: Reactome
GO - Biological processi
- antimicrobial humoral response Source: Reactome
- collagen catabolic process Source: UniProtKB
- digestion Source: UniProtKB-KW
- extracellular matrix disassembly Source: Reactome
- positive regulation of cell adhesion Source: UniProtKB
- positive regulation of cell growth Source: UniProtKB
- proteolysis Source: UniProtKB
Keywordsi
Molecular function | Hydrolase, Protease, Serine protease |
Biological process | Digestion |
Ligand | Calcium, Metal-binding |
Enzyme and pathway databases
PathwayCommonsi | P07478 |
Reactomei | R-HSA-1442490, Collagen degradation R-HSA-1462054, Alpha-defensins R-HSA-1592389, Activation of Matrix Metalloproteinases R-HSA-6798695, Neutrophil degranulation R-HSA-6803157, Antimicrobial peptides |
SignaLinki | P07478 |
SIGNORi | P07478 |
Protein family/group databases
MEROPSi | S01.258 |
Names & Taxonomyi
Protein namesi | Recommended name: Trypsin-2 (EC:3.4.21.4)Alternative name(s): Anionic trypsinogen Serine protease 2 Trypsin II |
Gene namesi | Name:PRSS2 Synonyms:TRY2, TRYP2 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:9483, PRSS2 |
MIMi | 601564, gene |
neXtProti | NX_P07478 |
VEuPathDBi | HostDB:ENSG00000275896 |
Subcellular locationi
Extracellular region or secreted
Extracellular region or secreted
- extracellular region Source: UniProtKB
- extracellular space Source: UniProtKB
Lysosome
- azurophil granule lumen Source: Reactome
Other locations
- extracellular matrix Source: UniProtKB
Keywords - Cellular componenti
SecretedPathology & Biotechi
Organism-specific databases
DisGeNETi | 5645 |
MalaCardsi | PRSS2 |
OpenTargetsi | ENSG00000275896 |
Orphaneti | 676, Hereditary chronic pancreatitis |
PharmGKBi | PA33833 |
Miscellaneous databases
Pharosi | P07478, Tchem |
Chemistry databases
ChEMBLi | CHEMBL3159 |
DrugBanki | DB04410, 3-Phenylpropylamine DB03127, Benzamidine DB02464, Benzylamine DB01805, Monoisopropylphosphorylserine DB01973, O-Benzylsulfonyl-Serine DB04325, Phenethylamine DB03976, Phosphorylisopropane |
DrugCentrali | P07478 |
Genetic variation databases
BioMutai | PRSS2 |
DMDMi | 136413 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 15 | 1 PublicationAdd BLAST | 15 | |
PropeptideiPRO_0000028199 | 16 – 23 | Activation peptide | 8 | |
ChainiPRO_0000028200 | 24 – 247 | Trypsin-2Add BLAST | 224 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 30 ↔ 160 | PROSITE-ProRule annotation | ||
Disulfide bondi | 48 ↔ 64 | PROSITE-ProRule annotation | ||
Modified residuei | 154 | Sulfotyrosine2 Publications | 1 | |
Disulfide bondi | 171 ↔ 185 | PROSITE-ProRule annotation | ||
Disulfide bondi | 196 ↔ 220 | PROSITE-ProRule annotation |
Post-translational modificationi
Sulfated on tyrosine.1 Publication
Sulfation at Tyr-154 increases selectivity towards basic versus apolar residues at the P2' position of inhibitors that bind in a substrate-like fashion. Although the increase in selectivity is relatively small, it may facilitate digestion of a broader range of dietary proteins.1 Publication
Keywords - PTMi
Disulfide bond, Sulfation, ZymogenProteomic databases
jPOSTi | P07478 |
MassIVEi | P07478 |
PeptideAtlasi | P07478 |
PRIDEi | P07478 |
ProteomicsDBi | 52007 |
TopDownProteomicsi | P07478 |
PTM databases
iPTMneti | P07478 |
PhosphoSitePlusi | P07478 |
Expressioni
Tissue specificityi
Expressed in Paneth cells, at the base of small intestinal crypts.1 Publication
Gene expression databases
Bgeei | ENSG00000275896, Expressed in body of pancreas and 96 other tissues |
ExpressionAtlasi | P07478, baseline and differential |
Genevisiblei | P07478, HS |
Organism-specific databases
HPAi | ENSG00000275896, Tissue enriched (pancreas) |
Interactioni
Protein-protein interaction databases
BioGRIDi | 111627, 30 interactors |
IntActi | P07478, 13 interactors |
MINTi | P07478 |
STRINGi | 9606.ENSP00000485444 |
Chemistry databases
BindingDBi | P07478 |
Miscellaneous databases
RNActi | P07478, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 24 – 244 | Peptidase S1PROSITE-ProRule annotationAdd BLAST | 221 |
Sequence similaritiesi
Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG3627, Eukaryota |
GeneTreei | ENSGT00990000203555 |
InParanoidi | P07478 |
PhylomeDBi | P07478 |
Family and domain databases
CDDi | cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits |
InterProi | View protein in InterPro IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS IPR033116, TRYPSIN_SER |
Pfami | View protein in Pfam PF00089, Trypsin, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit |
PROSITEi | View protein in PROSITE PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit PS00135, TRYPSIN_SER, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
P07478-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNLLLILTFV AAAVAAPFDD DDKIVGGYIC EENSVPYQVS LNSGYHFCGG
60 70 80 90 100
SLISEQWVVS AGHCYKSRIQ VRLGEHNIEV LEGNEQFINA AKIIRHPKYN
110 120 130 140 150
SRTLDNDILL IKLSSPAVIN SRVSAISLPT APPAAGTESL ISGWGNTLSS
160 170 180 190 200
GADYPDELQC LDAPVLSQAE CEASYPGKIT NNMFCVGFLE GGKDSCQGDS
210 220 230 240
GGPVVSNGEL QGIVSWGYGC AQKNRPGVYT KVYNYVDWIK DTIAANS
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA6XMV9 | A6XMV9_HUMAN | Protease serine 2 preproprotein | PRSS2 | 261 | Annotation score: | ||
A0A0J9YYC8 | A0A0J9YYC8_HUMAN | Trypsin-2 | PRSS2 | 247 | Annotation score: | ||
A6XMV8 | A6XMV8_HUMAN | Protease serine 2 preproprotein | PRSS2 | 246 | Annotation score: |
Polymorphismi
His-153 variation is a common polymorphism in African populations with a minor allele frequency of 9.2%, it eliminates sulfation at Tyr-154, with no consequences on digestive physiology.1 Publication
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_051858 | 117 | A → V. Corresponds to variant dbSNP:rs11547028Ensembl. | 1 | |
Natural variantiVAR_071761 | 153 | D → H Abolishes tyrosine sulfation. 1 PublicationCorresponds to variant dbSNP:rs1804564Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M27602 mRNA Translation: AAA61232.1 |
CCDSi | CCDS83236.1 |
PIRi | B25852 |
RefSeqi | NP_002761.1, NM_002770.3 |
Genome annotation databases
Ensembli | ENST00000539842; ENSP00000488338; ENSG00000275896 ENST00000632112; ENSP00000487952; ENSG00000282049 |
GeneIDi | 5645 |
KEGGi | hsa:5645 |
MANE-Selecti | ENST00000539842.6; ENSP00000488338.1; NM_002770.4; NP_002761.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M27602 mRNA Translation: AAA61232.1 |
CCDSi | CCDS83236.1 |
PIRi | B25852 |
RefSeqi | NP_002761.1, NM_002770.3 |
3D structure databases
SMRi | P07478 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 111627, 30 interactors |
IntActi | P07478, 13 interactors |
MINTi | P07478 |
STRINGi | 9606.ENSP00000485444 |
Chemistry databases
BindingDBi | P07478 |
ChEMBLi | CHEMBL3159 |
DrugBanki | DB04410, 3-Phenylpropylamine DB03127, Benzamidine DB02464, Benzylamine DB01805, Monoisopropylphosphorylserine DB01973, O-Benzylsulfonyl-Serine DB04325, Phenethylamine DB03976, Phosphorylisopropane |
DrugCentrali | P07478 |
Protein family/group databases
MEROPSi | S01.258 |
PTM databases
iPTMneti | P07478 |
PhosphoSitePlusi | P07478 |
Genetic variation databases
BioMutai | PRSS2 |
DMDMi | 136413 |
Proteomic databases
jPOSTi | P07478 |
MassIVEi | P07478 |
PeptideAtlasi | P07478 |
PRIDEi | P07478 |
ProteomicsDBi | 52007 |
TopDownProteomicsi | P07478 |
Protocols and materials databases
Antibodypediai | 74627, 154 antibodies from 21 providers |
DNASUi | 5645 |
Genome annotation databases
Ensembli | ENST00000539842; ENSP00000488338; ENSG00000275896 ENST00000632112; ENSP00000487952; ENSG00000282049 |
GeneIDi | 5645 |
KEGGi | hsa:5645 |
MANE-Selecti | ENST00000539842.6; ENSP00000488338.1; NM_002770.4; NP_002761.1 |
Organism-specific databases
CTDi | 5645 |
DisGeNETi | 5645 |
GeneCardsi | PRSS2 |
HGNCi | HGNC:9483, PRSS2 |
HPAi | ENSG00000275896, Tissue enriched (pancreas) |
MalaCardsi | PRSS2 |
MIMi | 601564, gene |
neXtProti | NX_P07478 |
OpenTargetsi | ENSG00000275896 |
Orphaneti | 676, Hereditary chronic pancreatitis |
PharmGKBi | PA33833 |
VEuPathDBi | HostDB:ENSG00000275896 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3627, Eukaryota |
GeneTreei | ENSGT00990000203555 |
InParanoidi | P07478 |
PhylomeDBi | P07478 |
Enzyme and pathway databases
PathwayCommonsi | P07478 |
Reactomei | R-HSA-1442490, Collagen degradation R-HSA-1462054, Alpha-defensins R-HSA-1592389, Activation of Matrix Metalloproteinases R-HSA-6798695, Neutrophil degranulation R-HSA-6803157, Antimicrobial peptides |
SignaLinki | P07478 |
SIGNORi | P07478 |
Miscellaneous databases
BioGRID-ORCSi | 5645, 1 hit in 138 CRISPR screens |
ChiTaRSi | PRSS2, human |
GenomeRNAii | 5645 |
Pharosi | P07478, Tchem |
PROi | PR:P07478 |
RNActi | P07478, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000275896, Expressed in body of pancreas and 96 other tissues |
ExpressionAtlasi | P07478, baseline and differential |
Genevisiblei | P07478, HS |
Family and domain databases
CDDi | cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits |
InterProi | View protein in InterPro IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS IPR033116, TRYPSIN_SER |
Pfami | View protein in Pfam PF00089, Trypsin, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit |
PROSITEi | View protein in PROSITE PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit PS00135, TRYPSIN_SER, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TRY2_HUMAN | |
Accessioni | P07478Primary (citable) accession number: P07478 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1988 |
Last sequence update: | April 1, 1988 | |
Last modified: | February 23, 2022 | |
This is version 196 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - Human chromosome 7
Human chromosome 7: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families