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Protein

Carbonic anhydrase 3

Gene

CA3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Reversible hydration of carbon dioxide.

Catalytic activityi

H2CO3 = CO2 + H2O.

Cofactori

Zn2+2 Publications

Activity regulationi

Activated by proton donors such as imidazole and the dipeptide histidylhistidine. Inhibited by coumarins and sulfonamide derivatives such as acetazolamide.3 Publications

Kineticsi

  1. KM=52.0 mM for CO21 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi94Zinc; catalytic2 Publications1
    Metal bindingi96Zinc; catalytic2 Publications1
    Metal bindingi119Zinc; catalytic2 Publications1
    Active sitei127By similarity1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionLyase
    LigandMetal-binding, Zinc

    Enzyme and pathway databases

    BRENDAi4.2.1.1 2681
    ReactomeiR-HSA-1475029 Reversible hydration of carbon dioxide

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Carbonic anhydrase 3 (EC:4.2.1.1)
    Alternative name(s):
    Carbonate dehydratase III
    Carbonic anhydrase III
    Short name:
    CA-III
    Gene namesi
    Name:CA3
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 8

    Organism-specific databases

    EuPathDBiHostDB:ENSG00000164879.6
    HGNCiHGNC:1374 CA3
    MIMi114750 gene
    neXtProtiNX_P07451

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi64K → H: Enhanced proton transfer in catalysis. 1 Publication1
    Mutagenesisi67R → H: Enhanced proton transfer in catalysis. 1 Publication1
    Mutagenesisi197F → L: Enhanced activity by at least 10-fold. 1 Publication1

    Organism-specific databases

    DisGeNETi761
    OpenTargetsiENSG00000164879
    PharmGKBiPA25990

    Chemistry databases

    ChEMBLiCHEMBL2885
    DrugBankiDB00819 Acetazolamide
    DB08846 Ellagic Acid
    DB00909 Zonisamide

    Polymorphism and mutation databases

    BioMutaiCA3
    DMDMi134047703

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Initiator methionineiRemovedBy similarity
    ChainiPRO_00000774262 – 260Carbonic anhydrase 3Add BLAST259

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei2N-acetylalanineBy similarity1
    Modified residuei29PhosphoserineBy similarity1
    Modified residuei43PhosphoserineBy similarity1
    Modified residuei48PhosphoserineBy similarity1
    Modified residuei50PhosphoserineBy similarity1
    Modified residuei55PhosphoserineBy similarity1
    Modified residuei73PhosphothreonineBy similarity1
    Modified residuei127PhosphotyrosineBy similarity1
    Modified residuei129PhosphothreonineBy similarity1
    Modified residuei176PhosphothreonineBy similarity1
    Modified residuei182S-glutathionyl cysteineBy similarity1
    Modified residuei187S-glutathionyl cysteineBy similarity1
    Modified residuei216PhosphothreonineBy similarity1
    Modified residuei219PhosphoserineBy similarity1

    Post-translational modificationi

    S-glutathionylated in hepatocytes under oxidative stress.By similarity

    Keywords - PTMi

    Acetylation, Glutathionylation, Phosphoprotein

    Proteomic databases

    EPDiP07451
    PaxDbiP07451
    PeptideAtlasiP07451
    PRIDEiP07451
    ProteomicsDBi52004

    2D gel databases

    UCD-2DPAGEiP07451

    PTM databases

    iPTMnetiP07451
    PhosphoSitePlusiP07451

    Expressioni

    Tissue specificityi

    Muscle specific.

    Developmental stagei

    At 6 weeks gestation, transcripts accumulate at low levels in the somites and at high levels throughout the notochord. As gestation continues, CA3 becomes abundant in all developing muscle masses and continues at high to moderate levels in the notochord.1 Publication

    Gene expression databases

    BgeeiENSG00000164879 Expressed in 158 organ(s), highest expression level in vastus lateralis
    CleanExiHS_CA3
    ExpressionAtlasiP07451 baseline and differential
    GenevisibleiP07451 HS

    Organism-specific databases

    HPAiCAB004967
    HPA021775
    HPA026700

    Interactioni

    Protein-protein interaction databases

    BioGridi107216, 4 interactors
    IntActiP07451, 2 interactors
    STRINGi9606.ENSP00000285381

    Chemistry databases

    BindingDBiP07451

    Structurei

    Secondary structure

    1260
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details

    3D structure databases

    ProteinModelPortaliP07451
    SMRiP07451
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP07451

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini3 – 259Alpha-carbonic anhydrasePROSITE-ProRule annotationAdd BLAST257

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni64 – 67Involved in proton transfer4
    Regioni198 – 199Substrate bindingBy similarity2

    Sequence similaritiesi

    Belongs to the alpha-carbonic anhydrase family.Curated

    Phylogenomic databases

    eggNOGiKOG0382 Eukaryota
    COG3338 LUCA
    GeneTreeiENSGT00760000118915
    HOGENOMiHOG000112637
    HOVERGENiHBG002837
    InParanoidiP07451
    KOiK01672
    OMAiRDYWTYQ
    OrthoDBiEOG091G0XFM
    PhylomeDBiP07451
    TreeFamiTF316425

    Family and domain databases

    Gene3Di3.10.200.10, 1 hit
    InterProiView protein in InterPro
    IPR001148 CA_dom
    IPR036398 CA_dom_sf
    IPR023561 Carbonic_anhydrase_a-class
    IPR018338 Carbonic_anhydrase_a-class_CS
    IPR018441 Carbonic_anhydrase_CA3
    PANTHERiPTHR18952 PTHR18952, 1 hit
    PTHR18952:SF127 PTHR18952:SF127, 1 hit
    PfamiView protein in Pfam
    PF00194 Carb_anhydrase, 1 hit
    SMARTiView protein in SMART
    SM01057 Carb_anhydrase, 1 hit
    SUPFAMiSSF51069 SSF51069, 1 hit
    PROSITEiView protein in PROSITE
    PS00162 ALPHA_CA_1, 1 hit
    PS51144 ALPHA_CA_2, 1 hit

    Sequence (1+)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry has 1 described isoform and 1 potential isoform that is computationally mapped.iShow all

    P07451-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MAKEWGYASH NGPDHWHELF PNAKGENQSP VELHTKDIRH DPSLQPWSVS
    60 70 80 90 100
    YDGGSAKTIL NNGKTCRVVF DDTYDRSMLR GGPLPGPYRL RQFHLHWGSS
    110 120 130 140 150
    DDHGSEHTVD GVKYAAELHL VHWNPKYNTF KEALKQRDGI AVIGIFLKIG
    160 170 180 190 200
    HENGEFQIFL DALDKIKTKG KEAPFTKFDP SCLFPACRDY WTYQGSFTTP
    210 220 230 240 250
    PCEECIVWLL LKEPMTVSSD QMAKLRSLLS SAENEPPVPL VSNWRPPQPI
    260
    NNRVVRASFK
    Length:260
    Mass (Da):29,557
    Last modified:March 20, 2007 - v3
    Checksum:iA305334167233FCF
    GO

    Computationally mapped potential isoform sequencesi

    There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
    EntryEntry nameProtein names
    Gene namesLengthAnnotation
    E5RHI4E5RHI4_HUMAN
    Carbonic anhydrase 3
    CA3
    19Annotation score:

    Natural variant

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Natural variantiVAR_01618031V → I3 PublicationsCorresponds to variant dbSNP:rs20571Ensembl.1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M29458 Genomic DNA Translation: AAA52293.1
    M29452 Genomic DNA No translation available.
    AK096880 mRNA Translation: BAG53390.1
    AK313254 mRNA Translation: BAG36064.1
    CH471068 Genomic DNA Translation: EAW87133.1
    BC004897 mRNA Translation: AAH04897.1
    AJ006473 Genomic DNA Translation: CAA07056.1
    CCDSiCCDS6238.1
    PIRiA26658 CRHU3
    RefSeqiNP_005172.1, NM_005181.3
    UniGeneiHs.82129

    Genome annotation databases

    EnsembliENST00000285381; ENSP00000285381; ENSG00000164879
    GeneIDi761
    KEGGihsa:761

    Keywords - Coding sequence diversityi

    Polymorphism

    Similar proteinsi

    Entry informationi

    Entry nameiCAH3_HUMAN
    AccessioniPrimary (citable) accession number: P07451
    Secondary accession number(s): B2R867, B3KUC8, O60842
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
    Last sequence update: March 20, 2007
    Last modified: September 12, 2018
    This is version 187 of the entry and version 3 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome
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    Main funding by: National Institutes of Health

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