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UniProtKB - P07445 (SDIS_PSEPU)
Protein
Steroid Delta-isomerase
Gene
ksi
Organism
Pseudomonas putida (Arthrobacter siderocapsulatus)
Status
Functioni
Catalytic activityi
- EC:5.3.3.1
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 16 | Proton donor | 1 | |
Active sitei | 40 | Proton acceptor | 1 | |
Binding sitei | 103 | Substrate1 Publication | 1 |
GO - Molecular functioni
- steroid delta-isomerase activity Source: UniProtKB-EC
GO - Biological processi
- steroid metabolic process Source: UniProtKB-KW
Keywordsi
Molecular function | Isomerase |
Biological process | Lipid metabolism, Steroid metabolism |
Enzyme and pathway databases
BRENDAi | 5.3.3.1, 5092 |
Names & Taxonomyi
Protein namesi | Recommended name: Steroid Delta-isomerase (EC:5.3.3.1)Alternative name(s): Delta(5)-3-ketosteroid isomerase |
Gene namesi | Name:ksi |
Organismi | Pseudomonas putida (Arthrobacter siderocapsulatus) |
Taxonomic identifieri | 303 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › |
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 16 | Y → F: Reduces activity 2000-fold. Reduces activity 10000-fold; when associated with E-103; N-103 or L-103. 3 Publications | 1 | |
Mutagenesisi | 16 | Y → S: Reduces activity 20-fold. 3 Publications | 1 | |
Mutagenesisi | 32 | Y → S: Reduces activity 4-fold. 1 Publication | 1 | |
Mutagenesisi | 57 | Y → S: Reduces activity 100-fold. | 1 | |
Mutagenesisi | 92 | W → A: Slightly reduces activity. Reduces protein stability. | 1 | |
Mutagenesisi | 103 | D → A or L: Reduces activity 100-fold. Reduces activity 10000-fold; when associated with F-16. 2 Publications | 1 | |
Mutagenesisi | 103 | D → E: Slightly reduces activity. Reduces activity 10000-fold; when associated with F-16. 2 Publications | 1 | |
Mutagenesisi | 103 | D → N: Reduces activity 4-fold. Reduces activity 10000-fold; when associated with F-16. 2 Publications | 1 | |
Mutagenesisi | 125 | L → A: Slightly reduces activity and reduces protein stability; when associated with A-127. 1 Publication | 1 | |
Mutagenesisi | 127 | V → A: Slightly reduces activity and reduces protein stability; when associated with A-125. 1 Publication | 1 |
Chemistry databases
ChEMBLi | CHEMBL2321641 |
DrugBanki | DB03619, Deoxycholic acid DB03515, Equilenin |
DrugCentrali | P07445 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000097646 | 1 – 131 | Steroid Delta-isomeraseAdd BLAST | 131 |
Expressioni
Inductioni
By steroids.
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P07445 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P07445 |
Family & Domainsi
Family and domain databases
CDDi | cd00781, ketosteroid_isomerase, 1 hit |
InterProi | View protein in InterPro IPR039256, Ketosteroid_isomerase IPR032710, NTF2-like_dom_sf IPR037401, SnoaL-like |
Pfami | View protein in Pfam PF12680, SnoaL_2, 1 hit |
SUPFAMi | SSF54427, SSF54427, 1 hit |
i Sequence
Sequence statusi: Complete.
P07445-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNLPTAQEVQ GLMARYIELV DVGDIEAIVQ MYADDATVED PFGQPPIHGR
60 70 80 90 100
EQIAAFYRQG LGGGKVRACL TGPVRASHNG CGAMPFRVEM VWNGQPCALD
110 120 130
VIDVMRFDEH GRIQTMQAYW SEVNLSVREP Q
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L13127 Genomic DNA Translation: AAA64437.1 |
PIRi | A25216, SIPSDP |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L13127 Genomic DNA Translation: AAA64437.1 |
PIRi | A25216, SIPSDP |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1C7H | X-ray | 2.50 | A | 1-131 | [»] | |
1CQS | X-ray | 1.90 | A/B | 1-131 | [»] | |
1DMM | X-ray | 1.90 | A | 1-131 | [»] | |
1DMN | X-ray | 2.05 | A | 1-131 | [»] | |
1DMQ | X-ray | 2.15 | A | 1-131 | [»] | |
1E3R | X-ray | 2.50 | A/B | 1-131 | [»] | |
1E3V | X-ray | 2.00 | A/B | 1-131 | [»] | |
1E97 | X-ray | 2.00 | A | 1-131 | [»] | |
1EA2 | X-ray | 1.80 | A | 1-131 | [»] | |
1GS3 | X-ray | 2.10 | A | 1-131 | [»] | |
1K41 | X-ray | 2.20 | A/B | 1-131 | [»] | |
1OGX | X-ray | 2.00 | A/B | 1-131 | [»] | |
1OH0 | X-ray | 1.10 | A/B | 1-131 | [»] | |
1OHO | X-ray | 1.90 | A | 1-131 | [»] | |
1OPY | X-ray | 1.90 | A | 1-131 | [»] | |
1VZZ | X-ray | 2.30 | A/B | 1-131 | [»] | |
1W00 | X-ray | 2.20 | A/B | 1-131 | [»] | |
1W01 | X-ray | 2.20 | A/B | 1-131 | [»] | |
1W02 | X-ray | 2.30 | A | 1-131 | [»] | |
1W6Y | X-ray | 2.10 | A | 1-131 | [»] | |
2INX | X-ray | 1.50 | A | 1-131 | [»] | |
2PZV | X-ray | 1.25 | A/B/C/D | 1-131 | [»] | |
3CPO | X-ray | 1.24 | A | 1-131 | [»] | |
3FZW | X-ray | 1.32 | A/B | 1-131 | [»] | |
3IPT | X-ray | 1.63 | A/B/C/D | 1-131 | [»] | |
3OWS | X-ray | 1.71 | A/B/C/D | 1-131 | [»] | |
3OWU | X-ray | 1.70 | A/B/C/D | 1-131 | [»] | |
3OWY | X-ray | 2.30 | A/B/C/D/E/F/G/H | 1-131 | [»] | |
3OX9 | X-ray | 2.00 | A/B/C/D | 1-131 | [»] | |
3OXA | X-ray | 1.89 | A/B/C/D | 1-131 | [»] | |
3RGR | X-ray | 1.59 | A | 1-131 | [»] | |
3SED | X-ray | 1.30 | A | 3-127 | [»] | |
3T8N | X-ray | 1.47 | A/B/D/F | 1-131 | [»] | |
3VGN | X-ray | 1.30 | A/B | 1-131 | [»] | |
3VSY | X-ray | 1.50 | A/B | 3-131 | [»] | |
4CDL | X-ray | 2.50 | A | 1-131 | [»] | |
4K1U | X-ray | 2.00 | A/B | 1-131 | [»] | |
4K1V | X-ray | 1.80 | A | 1-131 | [»] | |
5AI1 | X-ray | 2.10 | A | 1-131 | [»] | |
5D81 | X-ray | 1.39 | A | 1-131 | [»] | |
5D82 | X-ray | 1.37 | A/B | 1-131 | [»] | |
5D83 | X-ray | 1.70 | A/B | 1-131 | [»] | |
5G2G | X-ray | 1.60 | A/B | 2-128 | [»] | |
5KP1 | X-ray | 1.22 | A/B/C/D | 1-131 | [»] | |
5KP3 | X-ray | 1.70 | A/B | 1-131 | [»] | |
5KP4 | X-ray | 1.71 | A/B | 1-131 | [»] | |
6C17 | X-ray | 1.10 | A | 1-131 | [»] | |
6C1J | X-ray | 1.06 | A | 1-131 | [»] | |
6C1X | X-ray | 1.05 | A | 1-131 | [»] | |
6F4Y | X-ray | 1.92 | A/B | 3-127 | [»] | |
6F50 | X-ray | 2.00 | A/B | 3-127 | [»] | |
6F53 | X-ray | 1.49 | A | 3-127 | [»] | |
6F54 | X-ray | 1.08 | A/B | 3-127 | [»] | |
6TZD | X-ray | 1.45 | A/B | 1-131 | [»] | |
6U1Z | X-ray | 1.50 | A/B | 1-131 | [»] | |
6U4I | X-ray | 1.55 | A/B | 1-131 | [»] | |
6UBQ | X-ray | 1.30 | A/B | 1-131 | [»] | |
6UCN | X-ray | 1.32 | A/B | 1-131 | [»] | |
6UCW | X-ray | 1.25 | A/B | 1-131 | [»] | |
6UCY | X-ray | 1.15 | A/B | 1-131 | [»] | |
6UFS | X-ray | 1.47 | A/B | 1-131 | [»] | |
SMRi | P07445 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Chemistry databases
BindingDBi | P07445 |
ChEMBLi | CHEMBL2321641 |
DrugBanki | DB03619, Deoxycholic acid DB03515, Equilenin |
DrugCentrali | P07445 |
Enzyme and pathway databases
BRENDAi | 5.3.3.1, 5092 |
Miscellaneous databases
EvolutionaryTracei | P07445 |
Family and domain databases
CDDi | cd00781, ketosteroid_isomerase, 1 hit |
InterProi | View protein in InterPro IPR039256, Ketosteroid_isomerase IPR032710, NTF2-like_dom_sf IPR037401, SnoaL-like |
Pfami | View protein in Pfam PF12680, SnoaL_2, 1 hit |
SUPFAMi | SSF54427, SSF54427, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SDIS_PSEPU | |
Accessioni | P07445Primary (citable) accession number: P07445 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1988 |
Last sequence update: | April 1, 1988 | |
Last modified: | February 23, 2022 | |
This is version 118 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencingDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references