UniProtKB - P07445 (SDIS_PSEPU)

BLAST

>sp|P07445|SDIS_PSEPU Steroid Delta-isomerase OS=Pseudomonas putida OX=303 GN=ksi PE=1 SV=1
MNLPTAQEVQGLMARYIELVDVGDIEAIVQMYADDATVEDPFGQPPIHGREQIAAFYRQG
LGGGKVRACLTGPVRASHNGCGAMPFRVEMVWNGQPCALDVIDVMRFDEHGRIQTMQAYW
SEVNLSVREPQ

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History
Entry version 107 (07 Nov 2018)
Sequence version 1 (01 Apr 1988)
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Protein

Steroid Delta-isomerase

Gene

ksi

Organism

Pseudomonas putida (Arthrobacter siderocapsulatus)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

Catalytic activityⁱ

A 3-oxo-Delta₅-steroid = a 3-oxo-Delta₄-steroid.

Sites

Feature key	Position(s)	DescriptionActions	Length
Active siteⁱ	16	Proton donor	1
Active siteⁱ	40	Proton acceptor	1
Binding siteⁱ	103	Substrate1 Publication	1

GO - Molecular functionⁱ

steroid delta-isomerase activity Source: UniProtKB-EC

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

steroid metabolic process Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Isomerase
Biological process	Lipid metabolism, Steroid metabolism

Enzyme and pathway databases

BRENDAⁱ	5.3.3.1 5092

BRENDAⁱ

5.3.3.1 5092

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Steroid Delta-isomerase (EC:5.3.3.1) Alternative name(s): Delta(5)-3-ketosteroid isomerase
Gene namesⁱ	Name:ksi
Organismⁱ	Pseudomonas putida (Arthrobacter siderocapsulatus)
Taxonomic identifierⁱ	303 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas putida group

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	16	Y → F: Reduces activity 2000-fold. Reduces activity 10000-fold; when associated with E-103; N-103 or L-103. 3 Publications	1
Mutagenesisⁱ	16	Y → S: Reduces activity 20-fold. 3 Publications	1
Mutagenesisⁱ	32	Y → S: Reduces activity 4-fold. 1 Publication	1
Mutagenesisⁱ	57	Y → S: Reduces activity 100-fold.	1
Mutagenesisⁱ	92	W → A: Slightly reduces activity. Reduces protein stability.	1
Mutagenesisⁱ	103	D → A or L: Reduces activity 100-fold. Reduces activity 10000-fold; when associated with F-16. 2 Publications	1
Mutagenesisⁱ	103	D → E: Slightly reduces activity. Reduces activity 10000-fold; when associated with F-16. 2 Publications	1
Mutagenesisⁱ	103	D → N: Reduces activity 4-fold. Reduces activity 10000-fold; when associated with F-16. 2 Publications	1
Mutagenesisⁱ	125	L → A: Slightly reduces activity and reduces protein stability; when associated with A-127. 1 Publication	1
Mutagenesisⁱ	127	V → A: Slightly reduces activity and reduces protein stability; when associated with A-125. 1 Publication	1

Chemistry databases

ChEMBLⁱ	CHEMBL2321641
Drug and drug target database More...DrugBankⁱ	DB03619 Deoxycholic Acid DB03515 Equilenin DB01708 Prasterone

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000097646	1 – 131	Steroid Delta-isomeraseAdd BLAST		131

Proteomic databases

PRIDEⁱ	P07445

PRIDEⁱ

P07445

Expressionⁱ

Inductionⁱ

By steroids.

Interactionⁱ

Subunit structureⁱ

Homodimer.3 Publications

Chemistry databases

BindingDBⁱ

P07445

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	6 – 22	Combined sources	17
Helixⁱ	25 – 30	Combined sources	6
Beta strandⁱ	32 – 41	Combined sources	10
Helixⁱ	49 – 61	Combined sources	13
Beta strandⁱ	62 – 64	Combined sources	3
Beta strandⁱ	67 – 72	Combined sources	6
Beta strandⁱ	78 – 92	Combined sources	15
Beta strandⁱ	95 – 107	Combined sources	13
Beta strandⁱ	111 – 119	Combined sources	9
Helixⁱ	122 – 124	Combined sources	3
Beta strandⁱ	125 – 127	Combined sources	3

Show more details

3D structure databases

ProteinModelPortalⁱ	P07445
SMRⁱ	P07445
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	P07445

EvolutionaryTraceⁱ

P07445

Family & Domainsⁱ

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00781 ketosteroid_isomerase, 1 hit
InterProⁱ	View protein in InterPro IPR039256 Ketosteroid_isomerase IPR032710 NTF2-like_dom_sf IPR037401 SnoaL-like
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF12680 SnoaL_2, 1 hit
SUPFAMⁱ	SSF54427 SSF54427, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P07445-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MNLPTAQEVQ GLMARYIELV DVGDIEAIVQ MYADDATVED PFGQPPIHGR 
        60         70         80         90        100
EQIAAFYRQG LGGGKVRACL TGPVRASHNG CGAMPFRVEM VWNGQPCALD 
       110        120        130 
VIDVMRFDEH GRIQTMQAYW SEVNLSVREP Q

Length:131

Mass (Da):14,536

Last modified:April 1, 1988 - v1

Checksum:ⁱ08711198C13CF014

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	L13127 Genomic DNA Translation: AAA64437.1
PIRⁱ	A25216 SIPSDP

Protein	Similar proteins		Species	Score	Length	Source
P07445	Steroid Delta-isomerase		Pseudomonas wadenswilerensis		131	UniRef90_P07445
UPI00084A2E7D		PSEPU		135
UPI000719A0AE		PSEPU		135
UPI000719A0C1		PSEPU		135
UPI000719A0C2		PSEPU		135
+29

Protein	Similar proteins		Species	Score	Length	Source
P07445	Steroid Delta-isomerase		Pseudomonas wadenswilerensis		131	UniRef50_P07445
Steroid delta-isomerase		Pseudomonas citronellolis		127
Steroid delta-isomerase		Pseudomonas sp. 57B-090624		129
NTF2 domain-containing protein		Pseudomonas sp. GM49		129
Steroid delta-isomerase		Pseudomonas sp. GV041		129
+101

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	L13127 Genomic DNA Translation: AAA64437.1
PIRⁱ	A25216 SIPSDP

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1C7H	X-ray	2.50	A	1-131	[»]
	1CQS	X-ray	1.90	A/B	1-131	[»]
	1DMM	X-ray	1.90	A	1-131	[»]
	1DMN	X-ray	2.05	A	1-131	[»]
	1DMQ	X-ray	2.15	A	1-131	[»]
	1E3R	X-ray	2.50	A/B	1-131	[»]
	1E3V	X-ray	2.00	A/B	1-131	[»]
	1E97	X-ray	2.00	A	1-131	[»]
	1EA2	X-ray	1.80	A	1-131	[»]
	1GS3	X-ray	2.10	A	1-131	[»]
	1K41	X-ray	2.20	A/B	1-131	[»]
	1OGX	X-ray	2.00	A/B	1-131	[»]
	1OH0	X-ray	1.10	A/B	1-131	[»]
	1OHO	X-ray	1.90	A	1-131	[»]
	1OPY	X-ray	1.90	A	1-131	[»]
	1VZZ	X-ray	2.30	A/B	1-131	[»]
	1W00	X-ray	2.20	A/B	1-131	[»]
	1W01	X-ray	2.20	A/B	1-131	[»]
	1W02	X-ray	2.30	A	1-131	[»]
	1W6Y	X-ray	2.10	A	1-131	[»]
	2INX	X-ray	1.50	A	1-131	[»]
	2PZV	X-ray	1.25	A/B/C/D	1-131	[»]
	3CPO	X-ray	1.24	A	1-131	[»]
	3FZW	X-ray	1.32	A/B	1-131	[»]
	3IPT	X-ray	1.63	A/B/C/D	1-131	[»]
	3OWS	X-ray	1.71	A/B/C/D	1-131	[»]
	3OWU	X-ray	1.70	A/B/C/D	1-131	[»]
	3OWY	X-ray	2.30	A/B/C/D/E/F/G/H	1-131	[»]
	3OX9	X-ray	2.00	A/B/C/D	1-131	[»]
	3OXA	X-ray	1.89	A/B/C/D	1-131	[»]
	3RGR	X-ray	1.59	A	1-131	[»]
	3SED	X-ray	1.30	A	3-127	[»]
	3T8N	X-ray	1.47	A/B/D/F	1-131	[»]
	3VGN	X-ray	1.30	A/B	1-131	[»]
	3VSY	X-ray	1.50	A/B	3-131	[»]
	4CDL	X-ray	2.50	A	1-131	[»]
	4K1U	X-ray	2.00	A/B	1-131	[»]
	4K1V	X-ray	1.80	A	1-131	[»]
	5AI1	X-ray	2.10	A	1-131	[»]
	5D81	X-ray	1.39	A	1-131	[»]
	5D82	X-ray	1.37	A/B	1-131	[»]
	5D83	X-ray	1.70	A/B	1-131	[»]
	5G2G	X-ray	1.60	A/B	2-128	[»]
	5KP1	X-ray	1.22	A/B/C/D	1-131	[»]
	5KP3	X-ray	1.70	A/B	1-131	[»]
	5KP4	X-ray	1.71	A/B	1-131	[»]
	6C17	X-ray	1.10	A	1-131	[»]
	6C1J	X-ray	1.06	A	1-131	[»]
	6C1X	X-ray	1.05	A	1-131	[»]
	6F4Y	X-ray	1.92	A/B	3-127	[»]
	6F50	X-ray	2.00	A/B	3-127	[»]
	6F53	X-ray	1.49	A	3-127	[»]
	6F54	X-ray	1.08	A/B	3-127	[»]
ProteinModelPortalⁱ	P07445
SMRⁱ	P07445
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Chemistry databases

BindingDBⁱ	P07445
ChEMBLⁱ	CHEMBL2321641
DrugBankⁱ	DB03619 Deoxycholic Acid DB03515 Equilenin DB01708 Prasterone

Proteomic databases

PRIDEⁱ	P07445

PRIDEⁱ

P07445

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Enzyme and pathway databases

BRENDAⁱ	5.3.3.1 5092

BRENDAⁱ

5.3.3.1 5092

Miscellaneous databases

EvolutionaryTraceⁱ	P07445

EvolutionaryTraceⁱ

P07445

Family and domain databases

CDDⁱ	cd00781 ketosteroid_isomerase, 1 hit
InterProⁱ	View protein in InterPro IPR039256 Ketosteroid_isomerase IPR032710 NTF2-like_dom_sf IPR037401 SnoaL-like
Pfamⁱ	View protein in Pfam PF12680 SnoaL_2, 1 hit
SUPFAMⁱ	SSF54427 SSF54427, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	SDIS_PSEPU
Accessionⁱ	P07445Primary (citable) accession number: P07445
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
	Last sequence update:	April 1, 1988
	Last modified:	November 7, 2018
	This is version 107 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P07445 (SDIS_PSEPU)

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Steroid Delta-isomerase

ksi

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Chemistry databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Chemistry databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Family and domain databases

Sequence databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Chemistry databases

Proteomic databases

Protocols and materials databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ