UniProtKB - P07358 (CO8B_HUMAN)
Complement component C8 beta chain
C8B
Functioni
Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells.
GO - Molecular functioni
- protein-containing complex binding Source: Ensembl
GO - Biological processi
- complement activation Source: ProtInc
- complement activation, alternative pathway Source: UniProtKB-KW
- complement activation, classical pathway Source: UniProtKB-KW
- cytolysis Source: UniProtKB-KW
- immune response Source: ProtInc
Keywordsi
Biological process | Complement alternate pathway, Complement pathway, Cytolysis, Immunity, Innate immunity |
Enzyme and pathway databases
PathwayCommonsi | P07358 |
Reactomei | R-HSA-166665, Terminal pathway of complement R-HSA-977606, Regulation of Complement cascade |
SignaLinki | P07358 |
SIGNORi | P07358 |
Names & Taxonomyi
Protein namesi | Recommended name: Complement component C8 beta chainAlternative name(s): Complement component 8 subunit beta |
Gene namesi | Name:C8B |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:1353, C8B |
MIMi | 120960, gene |
neXtProti | NX_P07358 |
VEuPathDBi | HostDB:ENSG00000021852 |
Subcellular locationi
Extracellular region or secreted
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
- extracellular region Source: Reactome
- extracellular vesicle Source: UniProtKB
Plasma Membrane
- membrane attack complex Source: UniProtKB
Other locations
- membrane Source: ProtInc
Keywords - Cellular componenti
Membrane attack complex, SecretedPathology & Biotechi
Involvement in diseasei
Complement component 8 deficiency, 2 (C8D2)
Organism-specific databases
DisGeNETi | 732 |
MalaCardsi | C8B |
MIMi | 613789, phenotype |
Orphaneti | 169150, Immunodeficiency due to a late component of complement deficiency |
PharmGKBi | PA25952 |
Miscellaneous databases
Pharosi | P07358, Tbio |
Chemistry databases
DrugBanki | DB09130, Copper DB01593, Zinc DB14487, Zinc acetate |
Genetic variation databases
BioMutai | C8B |
DMDMi | 20141201 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 32 | Sequence analysisAdd BLAST | 32 | |
PropeptideiPRO_0000023591 | 33 – 54 | 1 PublicationAdd BLAST | 22 | |
ChainiPRO_0000023592 | 55 – 591 | Complement component C8 beta chainAdd BLAST | 537 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 65 ↔ 100 | 1 Publication | ||
Glycosylationi | 70 | C-linked (Man) tryptophan1 Publication | 1 | |
Glycosylationi | 73 | C-linked (Man) tryptophan1 Publication | 1 | |
Disulfide bondi | 76 ↔ 110 | 1 Publication | ||
Disulfide bondi | 79 ↔ 116 | 1 Publication | ||
Glycosylationi | 101 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 122 ↔ 133 | 1 Publication | ||
Disulfide bondi | 127 ↔ 146 | 1 Publication | ||
Disulfide bondi | 140 ↔ 155 | 1 Publication | ||
Glycosylationi | 243 | N-linked (GlcNAc...) asparagine2 Publications | 1 | |
Disulfide bondi | 378 ↔ 403 | 1 Publication | ||
Modified residuei | 418 | Phosphothreonine1 Publication | 1 | |
Disulfide bondi | 503 ↔ 550 | 1 Publication | ||
Disulfide bondi | 505 ↔ 521 | 1 Publication | ||
Disulfide bondi | 508 ↔ 523 | 1 Publication | ||
Disulfide bondi | 525 ↔ 534 | 1 Publication | ||
Glycosylationi | 551 | C-linked (Man) tryptophan1 Publication | 1 | |
Glycosylationi | 554 | C-linked (Man) tryptophan1 Publication | 1 | |
Disulfide bondi | 557 ↔ 590 | 1 Publication |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
jPOSTi | P07358 |
MassIVEi | P07358 |
PaxDbi | P07358 |
PeptideAtlasi | P07358 |
PRIDEi | P07358 |
ProteomicsDBi | 51998 |
PTM databases
GlyConnecti | 1149, 3 N-Linked glycans (1 site) |
GlyGeni | P07358, 6 sites, 4 N-linked glycans (1 site) |
iPTMneti | P07358 |
PhosphoSitePlusi | P07358 |
Expressioni
Gene expression databases
Bgeei | ENSG00000021852, Expressed in liver and 74 other tissues |
ExpressionAtlasi | P07358, baseline and differential |
Genevisiblei | P07358, HS |
Organism-specific databases
HPAi | ENSG00000021852, Tissue enriched (liver) |
Interactioni
Subunit structurei
Heterotrimer of 3 chains: alpha, beta and gamma. The alpha and gamma chains are disulfide bonded.
Component of the membrane attack complex (MAC). MAC assembly is initiated by proteolytic cleavage of C5 into C5a and C5b. C5b binds sequentially C6, C7, C8 and multiple copies of the pore-forming subunit C9.
2 PublicationsProtein-protein interaction databases
BioGRIDi | 107193, 6 interactors |
ComplexPortali | CPX-6159, Membrane attack complex |
IntActi | P07358, 1 interactor |
STRINGi | 9606.ENSP00000360281 |
Miscellaneous databases
RNActi | P07358, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P07358 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P07358 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 64 – 117 | TSP type-1 1PROSITE-ProRule annotationAdd BLAST | 54 | |
Domaini | 120 – 157 | LDL-receptor class APROSITE-ProRule annotationAdd BLAST | 38 | |
Domaini | 158 – 504 | MACPFPROSITE-ProRule annotationAdd BLAST | 347 | |
Domaini | 505 – 535 | EGF-likeAdd BLAST | 31 | |
Domaini | 545 – 591 | TSP type-1 2PROSITE-ProRule annotationAdd BLAST | 47 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 568 – 591 | DisorderedSequence analysisAdd BLAST | 24 |
Sequence similaritiesi
Keywords - Domaini
EGF-like domain, Repeat, SignalPhylogenomic databases
eggNOGi | KOG3535, Eukaryota |
InParanoidi | P07358 |
OrthoDBi | 787014at2759 |
PhylomeDBi | P07358 |
TreeFami | TF330498 |
Family and domain databases
CDDi | cd00112, LDLa, 1 hit |
Gene3Di | 2.20.100.10, 2 hits 4.10.400.10, 1 hit |
InterProi | View protein in InterPro IPR037566, Complement_C8_beta IPR036055, LDL_receptor-like_sf IPR023415, LDLR_class-A_CS IPR002172, LDrepeatLR_classA_rpt IPR001862, MAC_perforin IPR020864, MACPF IPR020863, MACPF_CS IPR000884, TSP1_rpt IPR036383, TSP1_rpt_sf |
PANTHERi | PTHR45742:SF5, PTHR45742:SF5, 1 hit |
Pfami | View protein in Pfam PF00057, Ldl_recept_a, 1 hit PF01823, MACPF, 1 hit PF00090, TSP_1, 2 hits |
PRINTSi | PR00764, COMPLEMENTC9 |
SMARTi | View protein in SMART SM00192, LDLa, 1 hit SM00457, MACPF, 1 hit SM00209, TSP1, 2 hits |
SUPFAMi | SSF57424, SSF57424, 1 hit SSF82895, SSF82895, 2 hits |
PROSITEi | View protein in PROSITE PS00022, EGF_1, 1 hit PS01209, LDLRA_1, 1 hit PS50068, LDLRA_2, 1 hit PS00279, MACPF_1, 1 hit PS51412, MACPF_2, 1 hit PS50092, TSP1, 2 hits |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MKNSRTWAWR APVELFLLCA ALGCLSLPGS RGERPHSFGS NAVNKSFAKS
60 70 80 90 100
RQMRSVDVTL MPIDCELSSW SSWTTCDPCQ KKRYRYAYLL QPSQFHGEPC
110 120 130 140 150
NFSDKEVEDC VTNRPCRSQV RCEGFVCAQT GRCVNRRLLC NGDNDCGDQS
160 170 180 190 200
DEANCRRIYK KCQHEMDQYW GIGSLASGIN LFTNSFEGPV LDHRYYAGGC
210 220 230 240 250
SPHYILNTRF RKPYNVESYT PQTQGKYEFI LKEYESYSDF ERNVTEKMAS
260 270 280 290 300
KSGFSFGFKI PGIFELGISS QSDRGKHYIR RTKRFSHTKS VFLHARSDLE
310 320 330 340 350
VAHYKLKPRS LMLHYEFLQR VKRLPLEYSY GEYRDLFRDF GTHYITEAVL
360 370 380 390 400
GGIYEYTLVM NKEAMERGDY TLNNVHACAK NDFKIGGAIE EVYVSLGVSV
410 420 430 440 450
GKCRGILNEI KDRNKRDTMV EDLVVLVRGG ASEHITTLAY QELPTADLMQ
460 470 480 490 500
EWGDAVQYNP AIIKVKVEPL YELVTATDFA YSSTVRQNMK QALEEFQKEV
510 520 530 540 550
SSCHCAPCQG NGVPVLKGSR CDCICPVGSQ GLACEVSYRK NTPIDGKWNC
560 570 580 590
WSNWSSCSGR RKTRQRQCNN PPPQNGGSPC SGPASETLDC S
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF5H7G1 | F5H7G1_HUMAN | Complement component 8 subunit beta | C8B | 539 | Annotation score: | ||
F5GY80 | F5GY80_HUMAN | Complement component 8 subunit beta | C8B | 529 | Annotation score: |
Polymorphismi
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_027649 | 108 | E → K. Corresponds to variant dbSNP:rs12067507Ensembl. | 1 | |
Natural variantiVAR_012642 | 117 | R → G in allotype C8B A. 2 PublicationsCorresponds to variant dbSNP:rs1013579Ensembl. | 1 | |
Natural variantiVAR_027650 | 261 | P → L. Corresponds to variant dbSNP:rs12085435Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M16973 mRNA Translation: AAA51862.1 AK313382 mRNA Translation: BAG36180.1 AL121998 Genomic DNA No translation available. CH471059 Genomic DNA Translation: EAX06641.1 BC130575 mRNA Translation: AAI30576.1 X04393 mRNA Translation: CAA27981.1 |
CCDSi | CCDS30730.1 |
PIRi | A43071, C8HUB |
RefSeqi | NP_000057.2, NM_000066.3 XP_016857724.1, XM_017002235.1 |
Genome annotation databases
Ensembli | ENST00000371237; ENSP00000360281; ENSG00000021852 |
GeneIDi | 732 |
KEGGi | hsa:732 |
MANE-Selecti | ENST00000371237.9; ENSP00000360281.4; NM_000066.4; NP_000057.3 |
UCSCi | uc001cyp.5, human |
Similar proteinsi
Cross-referencesi
Web resourcesi
C8Bbase C8B mutation db |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M16973 mRNA Translation: AAA51862.1 AK313382 mRNA Translation: BAG36180.1 AL121998 Genomic DNA No translation available. CH471059 Genomic DNA Translation: EAX06641.1 BC130575 mRNA Translation: AAI30576.1 X04393 mRNA Translation: CAA27981.1 |
CCDSi | CCDS30730.1 |
PIRi | A43071, C8HUB |
RefSeqi | NP_000057.2, NM_000066.3 XP_016857724.1, XM_017002235.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3OJY | X-ray | 2.51 | B | 55-591 | [»] | |
6H03 | electron microscopy | 5.60 | C | 55-591 | [»] | |
6H04 | electron microscopy | 5.60 | C | 55-591 | [»] | |
7NYC | electron microscopy | 3.50 | D | 55-591 | [»] | |
7NYD | electron microscopy | 3.30 | D | 55-591 | [»] | |
SMRi | P07358 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 107193, 6 interactors |
ComplexPortali | CPX-6159, Membrane attack complex |
IntActi | P07358, 1 interactor |
STRINGi | 9606.ENSP00000360281 |
Chemistry databases
DrugBanki | DB09130, Copper DB01593, Zinc DB14487, Zinc acetate |
PTM databases
GlyConnecti | 1149, 3 N-Linked glycans (1 site) |
GlyGeni | P07358, 6 sites, 4 N-linked glycans (1 site) |
iPTMneti | P07358 |
PhosphoSitePlusi | P07358 |
Genetic variation databases
BioMutai | C8B |
DMDMi | 20141201 |
Proteomic databases
jPOSTi | P07358 |
MassIVEi | P07358 |
PaxDbi | P07358 |
PeptideAtlasi | P07358 |
PRIDEi | P07358 |
ProteomicsDBi | 51998 |
Protocols and materials databases
Antibodypediai | 19370, 208 antibodies from 27 providers |
DNASUi | 732 |
Genome annotation databases
Ensembli | ENST00000371237; ENSP00000360281; ENSG00000021852 |
GeneIDi | 732 |
KEGGi | hsa:732 |
MANE-Selecti | ENST00000371237.9; ENSP00000360281.4; NM_000066.4; NP_000057.3 |
UCSCi | uc001cyp.5, human |
Organism-specific databases
CTDi | 732 |
DisGeNETi | 732 |
GeneCardsi | C8B |
HGNCi | HGNC:1353, C8B |
HPAi | ENSG00000021852, Tissue enriched (liver) |
MalaCardsi | C8B |
MIMi | 120960, gene 613789, phenotype |
neXtProti | NX_P07358 |
Orphaneti | 169150, Immunodeficiency due to a late component of complement deficiency |
PharmGKBi | PA25952 |
VEuPathDBi | HostDB:ENSG00000021852 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3535, Eukaryota |
InParanoidi | P07358 |
OrthoDBi | 787014at2759 |
PhylomeDBi | P07358 |
TreeFami | TF330498 |
Enzyme and pathway databases
PathwayCommonsi | P07358 |
Reactomei | R-HSA-166665, Terminal pathway of complement R-HSA-977606, Regulation of Complement cascade |
SignaLinki | P07358 |
SIGNORi | P07358 |
Miscellaneous databases
BioGRID-ORCSi | 732, 9 hits in 1035 CRISPR screens |
EvolutionaryTracei | P07358 |
GenomeRNAii | 732 |
Pharosi | P07358, Tbio |
PROi | PR:P07358 |
RNActi | P07358, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000021852, Expressed in liver and 74 other tissues |
ExpressionAtlasi | P07358, baseline and differential |
Genevisiblei | P07358, HS |
Family and domain databases
CDDi | cd00112, LDLa, 1 hit |
Gene3Di | 2.20.100.10, 2 hits 4.10.400.10, 1 hit |
InterProi | View protein in InterPro IPR037566, Complement_C8_beta IPR036055, LDL_receptor-like_sf IPR023415, LDLR_class-A_CS IPR002172, LDrepeatLR_classA_rpt IPR001862, MAC_perforin IPR020864, MACPF IPR020863, MACPF_CS IPR000884, TSP1_rpt IPR036383, TSP1_rpt_sf |
PANTHERi | PTHR45742:SF5, PTHR45742:SF5, 1 hit |
Pfami | View protein in Pfam PF00057, Ldl_recept_a, 1 hit PF01823, MACPF, 1 hit PF00090, TSP_1, 2 hits |
PRINTSi | PR00764, COMPLEMENTC9 |
SMARTi | View protein in SMART SM00192, LDLa, 1 hit SM00457, MACPF, 1 hit SM00209, TSP1, 2 hits |
SUPFAMi | SSF57424, SSF57424, 1 hit SSF82895, SSF82895, 2 hits |
PROSITEi | View protein in PROSITE PS00022, EGF_1, 1 hit PS01209, LDLRA_1, 1 hit PS50068, LDLRA_2, 1 hit PS00279, MACPF_1, 1 hit PS51412, MACPF_2, 1 hit PS50092, TSP1, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | CO8B_HUMAN | |
Accessioni | P07358Primary (citable) accession number: P07358 Secondary accession number(s): A1L4K7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1988 |
Last sequence update: | January 23, 2002 | |
Last modified: | February 23, 2022 | |
This is version 201 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families