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Protein

Fumarate hydratase class II

Gene

fumC

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the TCA cycle. Catalyzes the stereospecific interconversion of fumarate to L-malate.UniRule annotation

Miscellaneous

There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors.UniRule annotation

Catalytic activityi

(S)-malate = fumarate + H2O.UniRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from fumarate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Fumarate hydratase class II (fumC)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from fumarate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei185SubstrateUniRule annotation1
Active sitei186Proton donor/acceptorUniRule annotation1
Active sitei316UniRule annotation1
Binding sitei317SubstrateUniRule annotation1
Sitei329Important for catalytic activityUniRule annotation1

GO - Molecular functioni

  • fumarate hydratase activity Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processTricarboxylic acid cycle

Enzyme and pathway databases

BioCyciBSUB:BSU33040-MONOMER
UniPathwayi
UPA00223;UER01007

Names & Taxonomyi

Protein namesi
Recommended name:
Fumarate hydratase class IIUniRule annotation (EC:4.2.1.2UniRule annotation)
Short name:
Fumarase CUniRule annotation
Alternative name(s):
Aerobic fumaraseUniRule annotation
Iron-independent fumaraseUniRule annotation
Gene namesi
Name:fumCUniRule annotation
Synonyms:citG
Ordered Locus Names:BSU33040
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001612551 – 462Fumarate hydratase class IIAdd BLAST462

Proteomic databases

PaxDbiP07343
PRIDEiP07343

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

IntActiP07343, 2 interactors
MINTiP07343
STRINGi224308.Bsubs1_010100017931

Structurei

3D structure databases

ProteinModelPortaliP07343
SMRiP07343
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni97 – 99Substrate bindingUniRule annotation3
Regioni127 – 130Substrate binding (B site)UniRule annotation4
Regioni137 – 139Substrate bindingUniRule annotation3
Regioni322 – 324Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C9Q Bacteria
COG0114 LUCA
HOGENOMiHOG000061736
InParanoidiP07343
KOiK01679
OMAiRIEYDTF
PhylomeDBiP07343

Family and domain databases

CDDicd01362 Fumarase_classII, 1 hit
Gene3Di1.10.275.10, 1 hit
HAMAPiMF_00743 FumaraseC, 1 hit
InterProiView protein in InterPro
IPR005677 Fum_hydII
IPR024083 Fumarase/histidase_N
IPR018951 Fumarase_C_C
IPR020557 Fumarate_lyase_CS
IPR000362 Fumarate_lyase_fam
IPR022761 Fumarate_lyase_N
IPR008948 L-Aspartase-like
PANTHERiPTHR11444 PTHR11444, 1 hit
PfamiView protein in Pfam
PF10415 FumaraseC_C, 1 hit
PF00206 Lyase_1, 1 hit
PRINTSiPR00149 FUMRATELYASE
SUPFAMiSSF48557 SSF48557, 1 hit
TIGRFAMsiTIGR00979 fumC_II, 1 hit
PROSITEiView protein in PROSITE
PS00163 FUMARATE_LYASES, 1 hit

Sequencei

Sequence statusi: Complete.

P07343-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEYRIERDTM GEVKVPADKF WGAQTQRSKE NFKIGSEKMP MRVVKAFAIL
60 70 80 90 100
KRSTALANKR LGNLDAEKAE AIAAVCDDVL KGKYDDNFPL VVWQTGSGTQ
110 120 130 140 150
SNMNMNEVVA NRATALLKEK NSDQTIHPND DVNRSQSSND TFPTAMHVAA
160 170 180 190 200
VLAVYEQLVP ALDQLRNTLD EKAKAYNDIV KIGRTHLQDA TPLTLGQEIS
210 220 230 240 250
GWVHMLDRSK EMILEATDKM RALAIGGTAV GTGINAHPEF GELVSEEITK
260 270 280 290 300
LTGQTFSSSP NKFHALTSHD EITYAHGALK ALAADLMKIA NDVRWLASGP
310 320 330 340 350
RCGIGEIVIP ENEPGSSIMP GKVNPTQSEA LTMIAAQIMG NDATIGFAAS
360 370 380 390 400
QGNFELNVFK PVIIYNFLQS VQLLSDGMNS FHDKCAVGIE PNKETIQENL
410 420 430 440 450
SNSLMLVTAL NPHIGYENAA KIAKLAHKEG LTLKEAALKL ELLTEEQFNE
460
MVKPEDMVKP KA
Length:462
Mass (Da):50,532
Last modified:May 30, 2000 - v2
Checksum:i8D797D18FEA82C06
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti66A → V in CAA25849 (PubMed:3923430).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01701 Genomic DNA Translation: CAA25849.1
AJ223978 Genomic DNA Translation: CAA11749.1
AL009126 Genomic DNA Translation: CAB15294.1
M11918 Genomic DNA Translation: AAA22464.1
Z93941 Genomic DNA Translation: CAB07973.1
PIRiA23033 UFBSC8
RefSeqiNP_391184.1, NC_000964.3
WP_003228523.1, NZ_JNCM01000033.1

Genome annotation databases

EnsemblBacteriaiCAB15294; CAB15294; BSU33040
GeneIDi938591
KEGGibsu:BSU33040
PATRICifig|224308.179.peg.3581

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01701 Genomic DNA Translation: CAA25849.1
AJ223978 Genomic DNA Translation: CAA11749.1
AL009126 Genomic DNA Translation: CAB15294.1
M11918 Genomic DNA Translation: AAA22464.1
Z93941 Genomic DNA Translation: CAB07973.1
PIRiA23033 UFBSC8
RefSeqiNP_391184.1, NC_000964.3
WP_003228523.1, NZ_JNCM01000033.1

3D structure databases

ProteinModelPortaliP07343
SMRiP07343
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP07343, 2 interactors
MINTiP07343
STRINGi224308.Bsubs1_010100017931

Proteomic databases

PaxDbiP07343
PRIDEiP07343

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB15294; CAB15294; BSU33040
GeneIDi938591
KEGGibsu:BSU33040
PATRICifig|224308.179.peg.3581

Phylogenomic databases

eggNOGiENOG4105C9Q Bacteria
COG0114 LUCA
HOGENOMiHOG000061736
InParanoidiP07343
KOiK01679
OMAiRIEYDTF
PhylomeDBiP07343

Enzyme and pathway databases

UniPathwayi
UPA00223;UER01007

BioCyciBSUB:BSU33040-MONOMER

Family and domain databases

CDDicd01362 Fumarase_classII, 1 hit
Gene3Di1.10.275.10, 1 hit
HAMAPiMF_00743 FumaraseC, 1 hit
InterProiView protein in InterPro
IPR005677 Fum_hydII
IPR024083 Fumarase/histidase_N
IPR018951 Fumarase_C_C
IPR020557 Fumarate_lyase_CS
IPR000362 Fumarate_lyase_fam
IPR022761 Fumarate_lyase_N
IPR008948 L-Aspartase-like
PANTHERiPTHR11444 PTHR11444, 1 hit
PfamiView protein in Pfam
PF10415 FumaraseC_C, 1 hit
PF00206 Lyase_1, 1 hit
PRINTSiPR00149 FUMRATELYASE
SUPFAMiSSF48557 SSF48557, 1 hit
TIGRFAMsiTIGR00979 fumC_II, 1 hit
PROSITEiView protein in PROSITE
PS00163 FUMARATE_LYASES, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiFUMC_BACSU
AccessioniPrimary (citable) accession number: P07343
Secondary accession number(s): O32194
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: May 30, 2000
Last modified: November 7, 2018
This is version 136 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
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Main funding by: National Institutes of Health

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