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Protein

Acetolactate synthase catalytic subunit, mitochondrial

Gene

ILV2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Contains 1 molecule of FAD per monomer. The role of this cofactor is not clear considering that the reaction does not involve redox chemistry.
Present with 31900 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

2 pyruvate = 2-acetolactate + CO2.

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity
  • thiamine diphosphateBy similarityNote: Binds 1 thiamine pyrophosphate per subunit.By similarity

Activity regulationi

The regulatory subunit ILV6 stimulates enzymatic activity seven- to tenfold and confers sensitivity to inhibition by valine and activation by ATP.1 Publication

Pathwayi: L-isoleucine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-isoleucine from 2-oxobutanoate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Acetolactate synthase catalytic subunit, mitochondrial (ILV2), Acetolactate synthase small subunit, mitochondrial (ILV6)
  2. Ketol-acid reductoisomerase, mitochondrial (ILV5)
  3. Dihydroxy-acid dehydratase, mitochondrial (ILV3)
  4. Branched-chain-amino-acid aminotransferase, mitochondrial (BAT1), Branched-chain-amino-acid aminotransferase, cytosolic (BAT2)
This subpathway is part of the pathway L-isoleucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-isoleucine from 2-oxobutanoate, the pathway L-isoleucine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: L-valine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-valine from pyruvate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Acetolactate synthase catalytic subunit, mitochondrial (ILV2), Acetolactate synthase small subunit, mitochondrial (ILV6)
  2. Ketol-acid reductoisomerase, mitochondrial (ILV5)
  3. Dihydroxy-acid dehydratase, mitochondrial (ILV3)
  4. Branched-chain-amino-acid aminotransferase, mitochondrial (BAT1), Branched-chain-amino-acid aminotransferase, cytosolic (BAT2)
This subpathway is part of the pathway L-valine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-valine from pyruvate, the pathway L-valine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei139Thiamine pyrophosphate1
Binding sitei241FAD1 Publication1
Metal bindingi550Magnesium1
Metal bindingi577Magnesium1
Metal bindingi579Magnesium; via carbonyl oxygen1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi355 – 376FAD1 PublicationAdd BLAST22
Nucleotide bindingi407 – 426FAD1 PublicationAdd BLAST20

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processAmino-acid biosynthesis, Branched-chain amino acid biosynthesis
LigandMagnesium, Metal-binding, Thiamine pyrophosphate

Enzyme and pathway databases

BioCyciYEAST:MONOMER3O-29
BRENDAi2.2.1.6 984
UniPathwayi
UPA00047;UER00055

UPA00049;UER00059

Names & Taxonomyi

Protein namesi
Recommended name:
Acetolactate synthase catalytic subunit, mitochondrial (EC:2.2.1.6)
Alternative name(s):
Acetohydroxy-acid synthase catalytic subunit
Short name:
AHAS
Short name:
ALS
Gene namesi
Name:ILV2
Synonyms:SMR1
Ordered Locus Names:YMR108W
ORF Names:YM9718.07
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

SGDiS000004714 ILV2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1075095

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 90MitochondrionSequence analysisAdd BLAST90
ChainiPRO_000003566491 – 687Acetolactate synthase catalytic subunit, mitochondrialAdd BLAST597

Proteomic databases

MaxQBiP07342
PaxDbiP07342
PRIDEiP07342

PTM databases

CarbonylDBiP07342
iPTMnetiP07342

Interactioni

Subunit structurei

Homodimer. The acetolactate synthase complex contains the catalytic regulatory subunit ILV2 and the regulatory small subunit ILV6.1 Publication

Protein-protein interaction databases

BioGridi35284, 43 interactors
ComplexPortaliCPX-3034 Acetolactate synthase complex
DIPiDIP-1104N
IntActiP07342, 73 interactors
MINTiP07342
STRINGi4932.YMR108W

Chemistry databases

BindingDBiP07342

Structurei

Secondary structure

1687
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

DisProtiDP00398
ProteinModelPortaliP07342
SMRiP07342
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07342

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni499 – 579Thiamine pyrophosphate bindingAdd BLAST81

Sequence similaritiesi

Belongs to the TPP enzyme family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00550000075465
HOGENOMiHOG000258448
InParanoidiP07342
KOiK01652
OMAiQGMVRQW
OrthoDBiEOG092C14MZ

Family and domain databases

CDDicd02015 TPP_AHAS, 1 hit
InterProiView protein in InterPro
IPR012846 Acetolactate_synth_lsu
IPR039368 AHAS_TPP
IPR029035 DHS-like_NAD/FAD-binding_dom
IPR029061 THDP-binding
IPR012000 Thiamin_PyroP_enz_cen_dom
IPR012001 Thiamin_PyroP_enz_TPP-bd_dom
IPR000399 TPP-bd_CS
IPR011766 TPP_enzyme-bd_C
PfamiView protein in Pfam
PF02775 TPP_enzyme_C, 1 hit
PF00205 TPP_enzyme_M, 1 hit
PF02776 TPP_enzyme_N, 1 hit
SUPFAMiSSF52467 SSF52467, 1 hit
SSF52518 SSF52518, 2 hits
TIGRFAMsiTIGR00118 acolac_lg, 1 hit
PROSITEiView protein in PROSITE
PS00187 TPP_ENZYMES, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07342-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MIRQSTLKNF AIKRCFQHIA YRNTPAMRSV ALAQRFYSSS SRYYSASPLP
60 70 80 90 100
ASKRPEPAPS FNVDPLEQPA EPSKLAKKLR AEPDMDTSFV GLTGGQIFNE
110 120 130 140 150
MMSRQNVDTV FGYPGGAILP VYDAIHNSDK FNFVLPKHEQ GAGHMAEGYA
160 170 180 190 200
RASGKPGVVL VTSGPGATNV VTPMADAFAD GIPMVVFTGQ VPTSAIGTDA
210 220 230 240 250
FQEADVVGIS RSCTKWNVMV KSVEELPLRI NEAFEIATSG RPGPVLVDLP
260 270 280 290 300
KDVTAAILRN PIPTKTTLPS NALNQLTSRA QDEFVMQSIN KAADLINLAK
310 320 330 340 350
KPVLYVGAGI LNHADGPRLL KELSDRAQIP VTTTLQGLGS FDQEDPKSLD
360 370 380 390 400
MLGMHGCATA NLAVQNADLI IAVGARFDDR VTGNISKFAP EARRAAAEGR
410 420 430 440 450
GGIIHFEVSP KNINKVVQTQ IAVEGDATTN LGKMMSKIFP VKERSEWFAQ
460 470 480 490 500
INKWKKEYPY AYMEETPGSK IKPQTVIKKL SKVANDTGRH VIVTTGVGQH
510 520 530 540 550
QMWAAQHWTW RNPHTFITSG GLGTMGYGLP AAIGAQVAKP ESLVIDIDGD
560 570 580 590 600
ASFNMTLTEL SSAVQAGTPV KILILNNEEQ GMVTQWQSLF YEHRYSHTHQ
610 620 630 640 650
LNPDFIKLAE AMGLKGLRVK KQEELDAKLK EFVSTKGPVL LEVEVDKKVP
660 670 680
VLPMVAGGSG LDEFINFDPE VERQQTELRH KRTGGKH
Length:687
Mass (Da):74,937
Last modified:April 1, 1988 - v1
Checksum:iB03C4D0F38AA1362
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02549 Genomic DNA Translation: CAA26400.1
Z49702 Genomic DNA Translation: CAA89744.1
AY692995 Genomic DNA Translation: AAT93014.1
BK006946 Genomic DNA Translation: DAA10005.1
PIRiA23808 YCBYI
RefSeqiNP_013826.1, NM_001182608.1

Genome annotation databases

EnsemblFungiiYMR108W; YMR108W; YMR108W
GeneIDi855135
KEGGisce:YMR108W

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02549 Genomic DNA Translation: CAA26400.1
Z49702 Genomic DNA Translation: CAA89744.1
AY692995 Genomic DNA Translation: AAT93014.1
BK006946 Genomic DNA Translation: DAA10005.1
PIRiA23808 YCBYI
RefSeqiNP_013826.1, NM_001182608.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JSCX-ray2.60A/B58-687[»]
1N0HX-ray2.80A/B58-687[»]
1T9AX-ray2.59A/B58-687[»]
1T9BX-ray2.20A/B58-687[»]
1T9CX-ray2.34A/B58-687[»]
1T9DX-ray2.30A/B/C/D58-687[»]
5FEMX-ray2.17A/B58-687[»]
5IMSX-ray1.98A/B58-687[»]
5WKCX-ray2.33A/B/D/E58-687[»]
6BD3X-ray2.28A/B58-687[»]
6BD9X-ray1.98A/B58-687[»]
DisProtiDP00398
ProteinModelPortaliP07342
SMRiP07342
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35284, 43 interactors
ComplexPortaliCPX-3034 Acetolactate synthase complex
DIPiDIP-1104N
IntActiP07342, 73 interactors
MINTiP07342
STRINGi4932.YMR108W

Chemistry databases

BindingDBiP07342
ChEMBLiCHEMBL1075095

PTM databases

CarbonylDBiP07342
iPTMnetiP07342

Proteomic databases

MaxQBiP07342
PaxDbiP07342
PRIDEiP07342

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR108W; YMR108W; YMR108W
GeneIDi855135
KEGGisce:YMR108W

Organism-specific databases

SGDiS000004714 ILV2

Phylogenomic databases

GeneTreeiENSGT00550000075465
HOGENOMiHOG000258448
InParanoidiP07342
KOiK01652
OMAiQGMVRQW
OrthoDBiEOG092C14MZ

Enzyme and pathway databases

UniPathwayi
UPA00047;UER00055

UPA00049;UER00059

BioCyciYEAST:MONOMER3O-29
BRENDAi2.2.1.6 984

Miscellaneous databases

EvolutionaryTraceiP07342
PROiPR:P07342

Family and domain databases

CDDicd02015 TPP_AHAS, 1 hit
InterProiView protein in InterPro
IPR012846 Acetolactate_synth_lsu
IPR039368 AHAS_TPP
IPR029035 DHS-like_NAD/FAD-binding_dom
IPR029061 THDP-binding
IPR012000 Thiamin_PyroP_enz_cen_dom
IPR012001 Thiamin_PyroP_enz_TPP-bd_dom
IPR000399 TPP-bd_CS
IPR011766 TPP_enzyme-bd_C
PfamiView protein in Pfam
PF02775 TPP_enzyme_C, 1 hit
PF00205 TPP_enzyme_M, 1 hit
PF02776 TPP_enzyme_N, 1 hit
SUPFAMiSSF52467 SSF52467, 1 hit
SSF52518 SSF52518, 2 hits
TIGRFAMsiTIGR00118 acolac_lg, 1 hit
PROSITEiView protein in PROSITE
PS00187 TPP_ENZYMES, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiILVB_YEAST
AccessioniPrimary (citable) accession number: P07342
Secondary accession number(s): D6VZT1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: November 7, 2018
This is version 206 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
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Main funding by: National Institutes of Health

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