UniProtKB - P07335 (KCRB_RAT)
Protein
Creatine kinase B-type
Gene
Ckb
Organism
Rattus norvegicus (Rat)
Status
Functioni
Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
Catalytic activityi
- EC:2.7.3.2PROSITE-ProRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 130 | ATPPROSITE-ProRule annotation | 1 | |
Binding sitei | 132 | ATPPROSITE-ProRule annotation | 1 | |
Binding sitei | 191 | ATPPROSITE-ProRule annotation | 1 | |
Binding sitei | 232 | SubstrateBy similarity | 1 | |
Binding sitei | 236 | ATPPROSITE-ProRule annotation | 1 | |
Binding sitei | 285 | SubstrateBy similarity | 1 | |
Binding sitei | 292 | ATPPROSITE-ProRule annotation | 1 | |
Binding sitei | 320 | ATPPROSITE-ProRule annotation | 1 | |
Binding sitei | 335 | ATPPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 128 – 132 | ATPPROSITE-ProRule annotation | 5 | |
Nucleotide bindingi | 320 – 325 | ATPPROSITE-ProRule annotation | 6 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- creatine kinase activity Source: RGD
- kinase activity Source: GO_Central
- ubiquitin protein ligase binding Source: RGD
GO - Biological processi
- brain development Source: RGD
- cellular chloride ion homeostasis Source: RGD
- cerebellum development Source: RGD
- phosphocreatine biosynthetic process Source: GO_Central
Keywordsi
Molecular function | Kinase, Transferase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-RNO-71288, Creatine metabolism |
Protein family/group databases
MoonProti | P07335 |
Names & Taxonomyi
Protein namesi | Recommended name: Creatine kinase B-type (EC:2.7.3.2)Alternative name(s): B-CK Creatine kinase B chain Creatine phosphokinase M-type Short name: CPK-B |
Gene namesi | Name:Ckb Synonyms:Ckbb |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 2357, Ckb |
Subcellular locationi
Other locations
Cytosol
- cytosol Source: Ensembl
Extracellular region or secreted
- extracellular space Source: GO_Central
Nucleus
- nucleus Source: RGD
Other locations
- dendrite Source: RGD
- neuronal cell body Source: RGD
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000211970 | 1 – 381 | Creatine kinase B-typeAdd BLAST | 381 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 4 | PhosphoserineBy similarity | 1 | |
Modified residuei | 35 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 125 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 199 | PhosphoserineBy similarity | 1 | |
Modified residuei | 269 | 3'-nitrotyrosineBy similarity | 1 | |
Modified residuei | 309 | PhosphoserineCombined sources | 1 | |
Modified residuei | 322 | PhosphothreonineBy similarity | 1 |
Keywords - PTMi
Nitration, PhosphoproteinProteomic databases
jPOSTi | P07335 |
PaxDbi | P07335 |
PRIDEi | P07335 |
2D gel databases
World-2DPAGEi | 0004:P07335 |
PTM databases
CarbonylDBi | P07335 |
iPTMneti | P07335 |
PhosphoSitePlusi | P07335 |
Expressioni
Tissue specificityi
In the kidney localized primarily in the outer medulla in the thick ascending limb and distal convoluted tubule.1 Publication
Gene expression databases
Bgeei | ENSRNOG00000010872, Expressed in cerebellum and 21 other tissues |
Genevisiblei | P07335, RN |
Interactioni
Subunit structurei
Dimer of identical or non-identical chains, which can be either B (brain type) or M (muscle type). With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain.
By similarityGO - Molecular functioni
- ubiquitin protein ligase binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 246448, 2 interactors |
CORUMi | P07335 |
IntActi | P07335, 1 interactor |
MINTi | P07335 |
STRINGi | 10116.ENSRNOP00000015122 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 11 – 98 | Phosphagen kinase N-terminalPROSITE-ProRule annotationAdd BLAST | 88 | |
Domaini | 125 – 367 | Phosphagen kinase C-terminalPROSITE-ProRule annotationAdd BLAST | 243 |
Sequence similaritiesi
Belongs to the ATP:guanido phosphotransferase family.PROSITE-ProRule annotation
Phylogenomic databases
eggNOGi | KOG3581, Eukaryota |
GeneTreei | ENSGT01010000222526 |
HOGENOMi | CLU_019868_4_2_1 |
InParanoidi | P07335 |
OMAi | RGHEFMW |
OrthoDBi | 825025at2759 |
PhylomeDBi | P07335 |
TreeFami | TF314214 |
Family and domain databases
Gene3Di | 1.10.135.10, 1 hit |
InterProi | View protein in InterPro IPR000749, ATP-guanido_PTrfase IPR022415, ATP-guanido_PTrfase_AS IPR022414, ATP-guanido_PTrfase_cat IPR022413, ATP-guanido_PTrfase_N IPR036802, ATP-guanido_PTrfase_N_sf IPR014746, Gln_synth/guanido_kin_cat_dom |
PANTHERi | PTHR11547, PTHR11547, 1 hit |
Pfami | View protein in Pfam PF00217, ATP-gua_Ptrans, 1 hit PF02807, ATP-gua_PtransN, 1 hit |
SUPFAMi | SSF48034, SSF48034, 1 hit SSF55931, SSF55931, 1 hit |
PROSITEi | View protein in PROSITE PS00112, PHOSPHAGEN_KINASE, 1 hit PS51510, PHOSPHAGEN_KINASE_C, 1 hit PS51509, PHOSPHAGEN_KINASE_N, 1 hit |
i Sequence
Sequence statusi: Complete.
P07335-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPFSNSHNTQ KLRFPAEDEF PDLSSHNNHM AKVLTPELYA ELRAKCTPSG
60 70 80 90 100
FTLDDAIQTG VDNPGHPYIM TVGAVAGDEE SYDVFKDLFD PIIEDRHGGY
110 120 130 140 150
QPSDEHKTDL NPDNLQGGDD LDPNYVLSSR VRTGRSIRGF CLPPHCSRGE
160 170 180 190 200
RRAIEKLAVE ALSSLDGDLS GRYYALKSMT EAEQQQLIDD HFLFDKPVSP
210 220 230 240 250
LLLASGMARD WPDARGIWHN DNKTFLVWIN EEDHLRVISM QKGGNMKEVF
260 270 280 290 300
TRFCTGLTQI ETLFKSKNYE FMWNPHLGYI LTCPSNLGTG LRAGVHIKLP
310 320 330 340 350
HLGKHEKFSE VLKRLRLQKR GTGGVDTAAV GGVFDVSNAD RLGFSEVELV
360 370 380
QMVVDGVKLL IEMEQRLEQG QPIDDLMPAQ K
Sequence cautioni
The sequence AAH87656 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 18 | D → A in AAA40931 (PubMed:2284002).Curated | 1 | |
Sequence conflicti | 18 | D → A in AAA40933 (PubMed:2284002).Curated | 1 | |
Sequence conflicti | 183 – 184 | EQ → DE in AAA40930 (PubMed:3005113).Curated | 2 | |
Sequence conflicti | 183 – 184 | EQ → DE in AAA40932 (PubMed:2838389).Curated | 2 | |
Sequence conflicti | 206 | G → A in AAA40932 (PubMed:2838389).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M14400 mRNA Translation: AAA40930.1 M18668 Genomic DNA Translation: AAA40932.1 M57664 mRNA Translation: AAA40933.1 M57665 Genomic DNA Translation: AAA40931.1 BC065307 mRNA Translation: AAH65307.2 BC070955 mRNA Translation: AAH70955.2 BC087656 mRNA Translation: AAH87656.1 Different initiation. BC099814 mRNA Translation: AAH99814.2 BC127477 mRNA Translation: AAI27478.2 |
PIRi | A23980, KIRTCB |
RefSeqi | NP_036661.3, NM_012529.3 |
Genome annotation databases
Ensembli | ENSRNOT00000015122; ENSRNOP00000015122; ENSRNOG00000010872 |
GeneIDi | 24264 |
KEGGi | rno:24264 |
UCSCi | RGD:2357, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M14400 mRNA Translation: AAA40930.1 M18668 Genomic DNA Translation: AAA40932.1 M57664 mRNA Translation: AAA40933.1 M57665 Genomic DNA Translation: AAA40931.1 BC065307 mRNA Translation: AAH65307.2 BC070955 mRNA Translation: AAH70955.2 BC087656 mRNA Translation: AAH87656.1 Different initiation. BC099814 mRNA Translation: AAH99814.2 BC127477 mRNA Translation: AAI27478.2 |
PIRi | A23980, KIRTCB |
RefSeqi | NP_036661.3, NM_012529.3 |
3D structure databases
SMRi | P07335 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 246448, 2 interactors |
CORUMi | P07335 |
IntActi | P07335, 1 interactor |
MINTi | P07335 |
STRINGi | 10116.ENSRNOP00000015122 |
Chemistry databases
ChEMBLi | CHEMBL2176812 |
Protein family/group databases
MoonProti | P07335 |
PTM databases
CarbonylDBi | P07335 |
iPTMneti | P07335 |
PhosphoSitePlusi | P07335 |
2D gel databases
World-2DPAGEi | 0004:P07335 |
Proteomic databases
jPOSTi | P07335 |
PaxDbi | P07335 |
PRIDEi | P07335 |
Genome annotation databases
Ensembli | ENSRNOT00000015122; ENSRNOP00000015122; ENSRNOG00000010872 |
GeneIDi | 24264 |
KEGGi | rno:24264 |
UCSCi | RGD:2357, rat |
Organism-specific databases
CTDi | 1152 |
RGDi | 2357, Ckb |
Phylogenomic databases
eggNOGi | KOG3581, Eukaryota |
GeneTreei | ENSGT01010000222526 |
HOGENOMi | CLU_019868_4_2_1 |
InParanoidi | P07335 |
OMAi | RGHEFMW |
OrthoDBi | 825025at2759 |
PhylomeDBi | P07335 |
TreeFami | TF314214 |
Enzyme and pathway databases
Reactomei | R-RNO-71288, Creatine metabolism |
Miscellaneous databases
PROi | PR:P07335 |
Gene expression databases
Bgeei | ENSRNOG00000010872, Expressed in cerebellum and 21 other tissues |
Genevisiblei | P07335, RN |
Family and domain databases
Gene3Di | 1.10.135.10, 1 hit |
InterProi | View protein in InterPro IPR000749, ATP-guanido_PTrfase IPR022415, ATP-guanido_PTrfase_AS IPR022414, ATP-guanido_PTrfase_cat IPR022413, ATP-guanido_PTrfase_N IPR036802, ATP-guanido_PTrfase_N_sf IPR014746, Gln_synth/guanido_kin_cat_dom |
PANTHERi | PTHR11547, PTHR11547, 1 hit |
Pfami | View protein in Pfam PF00217, ATP-gua_Ptrans, 1 hit PF02807, ATP-gua_PtransN, 1 hit |
SUPFAMi | SSF48034, SSF48034, 1 hit SSF55931, SSF55931, 1 hit |
PROSITEi | View protein in PROSITE PS00112, PHOSPHAGEN_KINASE, 1 hit PS51510, PHOSPHAGEN_KINASE_C, 1 hit PS51509, PHOSPHAGEN_KINASE_N, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KCRB_RAT | |
Accessioni | P07335Primary (citable) accession number: P07335 Secondary accession number(s): A0JPK7 Q6P139 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1988 |
Last sequence update: | January 9, 2007 | |
Last modified: | December 2, 2020 | |
This is version 166 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families