UniProtKB - P07314 (GGT1_RAT)
Glutathione hydrolase 1 proenzyme
Ggt1
Functioni
Cleaves the gamma-glutamyl bond of extracellular glutathione (gamma-Glu-Cys-Gly), glutathione conjugates and other gamma-glutamyl compounds, such as leukotriene C4 (LTC4). The metabolism of glutathione by GGT1 releases free glutamate and the dipeptide cysteinyl-glycine, which is hydrolyzed to cysteine and glycine by dipeptidases. In the presence of high concentrations of dipeptides and some amino acids, can also catalyze a transpeptidation reaction, transferring the gamma-glutamyl moiety to an acceptor amino acid to form a new gamma-glutamyl compound. Contributes to cysteine homeostasis, glutathione homeostasis and in the conversion of the leukotriene LTC4 to LTD4.
By similarityCatalytic activityi
- an α-amino acid + an N-terminal (5-L-glutamyl)-[peptide] = 5-L-glutamyl amino acid + N-terminal L-α-aminoacyl-[peptide]1 PublicationEC:2.3.2.21 Publication
- EC:3.4.19.131 PublicationThis reaction proceeds in the forwardBy similarity direction.
- EC:3.4.19.131 PublicationThis reaction proceeds in the forwardBy similarity direction.
- EC:3.4.19.141 PublicationThis reaction proceeds in the forwardBy similarity direction.
Activity regulationi
Kineticsi
- KM=5.9 µM for leukotriene C41 Publication
- KM=5.7 µM for glutathione1 Publication
- KM=5.8 µM for gamma-glutamyl-p-anilide1 Publication
: glutathione metabolism Pathwayi
This protein is involved in the pathway glutathione metabolism, which is part of Sulfur metabolism.By similarityView all proteins of this organism that are known to be involved in the pathway glutathione metabolism and in Sulfur metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 106 | GlutamateBy similarity | 1 | |
Active sitei | 380 | NucleophileBy similarity | 1 | |
Binding sitei | 419 | GlutamateBy similarity | 1 | |
Binding sitei | 422 | GlutamateBy similarity | 1 | |
Binding sitei | 473 | Glutamate; via amide nitrogenBy similarity | 1 |
GO - Molecular functioni
- aminoacyltransferase activity Source: RGD
- glutathione hydrolase activity Source: UniProtKB
- hypoglycin A gamma-glutamyl transpeptidase activity Source: UniProtKB-EC
- leukotriene-C(4) hydrolase Source: RGD
- leukotriene C4 gamma-glutamyl transferase activity Source: UniProtKB-EC
- peptidyltransferase activity Source: RGD
GO - Biological processi
- aging Source: RGD
- cellular amino acid metabolic process Source: RGD
- cellular response to oxidative stress Source: RGD
- cysteine biosynthetic process Source: RGD
- fatty acid metabolic process Source: RGD
- glutamate metabolic process Source: UniProtKB
- glutathione biosynthetic process Source: RGD
- glutathione catabolic process Source: UniProtKB
- hepatoblast differentiation Source: RGD
- hepatocyte differentiation Source: RGD
- leukotriene D4 biosynthetic process Source: RGD
- leukotriene metabolic process Source: RGD
- liver regeneration Source: RGD
- peptide modification Source: RGD
- proteolysis Source: RGD
- regulation of immune system process Source: RGD
- regulation of inflammatory response Source: RGD
- response to alcohol Source: RGD
- response to estradiol Source: RGD
- response to lipopolysaccharide Source: RGD
- response to tumor necrosis factor Source: RGD
- spermatogenesis Source: RGD
- zymogen activation Source: UniProtKB
Keywordsi
Molecular function | Acyltransferase, Hydrolase, Protease, Transferase |
Biological process | Glutathione biosynthesis |
Ligand | Sialic acid |
Enzyme and pathway databases
BRENDAi | 2.3.2.2, 5301 |
Reactomei | R-RNO-174403, Glutathione synthesis and recycling R-RNO-2142691, Synthesis of Leukotrienes (LT) and Eoxins (EX) R-RNO-5423646, Aflatoxin activation and detoxification |
UniPathwayi | UPA00204 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Ggt1 Synonyms:Ggt |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 2683, Ggt1 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Single-pass type II membrane protein 1 Publication
Extracellular region or secreted
- extracellular space Source: RGD
Plasma Membrane
- integral component of plasma membrane Source: RGD
- plasma membrane Source: UniProtKB
Other locations
- vesicle Source: RGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 4 | Cytoplasmic1 Publication | 4 | |
Transmembranei | 5 – 26 | Helical; Signal-anchor for type II membrane protein1 PublicationAdd BLAST | 22 | |
Topological domaini | 27 – 568 | Extracellular1 PublicationAdd BLAST | 542 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000011064 | 1 – 379 | Glutathione hydrolase 1 heavy chainAdd BLAST | 379 | |
ChainiPRO_0000011065 | 380 – 568 | Glutathione hydrolase 1 light chain1 PublicationAdd BLAST | 189 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 49 ↔ 73 | By similarity | ||
Glycosylationi | 94 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 114 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 119 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 191 ↔ 195 | By similarity | ||
Glycosylationi | 229 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 343 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 427 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 510 | N-linked (GlcNAc...) asparagineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, Glycoprotein, ZymogenProteomic databases
PRIDEi | P07314 |
PTM databases
GlyConnecti | 170, 1 O-Linked glycan |
GlyGeni | P07314, 8 sites, 2 N-linked glycans (1 site), 2 O-linked glycans (1 site) |
iPTMneti | P07314 |
PhosphoSitePlusi | P07314 |
Expressioni
Tissue specificityi
Interactioni
Subunit structurei
Heterodimer composed of the light and heavy chains. The active site is located in the light chain.
By similarityChemistry databases
BindingDBi | P07314 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 398 – 400 | Glutamate bindingBy similarity | 3 | |
Regioni | 450 – 451 | Glutamate bindingBy similarity | 2 |
Sequence similaritiesi
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
InParanoidi | P07314 |
OMAi | CYSIDAR |
OrthoDBi | 1419292at2759 |
PhylomeDBi | P07314 |
Family and domain databases
Gene3Di | 1.10.246.130, 1 hit 3.60.20.40, 1 hit |
InterProi | View protein in InterPro IPR043138, GGT_lsub_C IPR000101, GGT_peptidase IPR043137, GGT_ssub IPR029055, Ntn_hydrolases_N |
PANTHERi | PTHR11686, PTHR11686, 1 hit |
SUPFAMi | SSF56235, SSF56235, 1 hit |
TIGRFAMsi | TIGR00066, g_glut_trans, 1 hit |
PROSITEi | View protein in PROSITE PS00462, G_GLU_TRANSPEPTIDASE, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MKNRFLVLGL VAVVLVFVII GLCIWLPTTS GKPDHVYSRA AVATDAKRCS
60 70 80 90 100
EIGRDMLQEG GSVVDAAIAS LLCMGLINAH SMGIGGGLFF TIYNSTTRKA
110 120 130 140 150
EVINAREMAP RLANTSMFNN SKDSEEGGLS VAVPGEIRGY ELAHQRHGRL
160 170 180 190 200
PWARLFQPSI QLARHGFPVG KGLARALDKK RDIIEKTPAL CEVFCRQGKV
210 220 230 240 250
LQEGETVTMP KLADTLQILA QEGARAFYNG SLTAQIVKDI QEAGGIMTVE
260 270 280 290 300
DLNNYRAEVI EHPMSIGLGD STLYVPSAPL SGPVLILILN ILKGYNFSPK
310 320 330 340 350
SVATPEQKAL TYHRIVEAFR FAYAKRTMLG DPKFVDVSQV IRNMSSEFYA
360 370 380 390 400
TQLRARITDE TTHPTAYYEP EFYLPDDGGT AHLSVVSEDG SAVAATSTIN
410 420 430 440 450
LYFGSKVLSR VSGILFNDEM DDFSSPNFTN QFGVAPSPAN FIKPGKQPLS
460 470 480 490 500
SMCPSIIVDK DGKVRMVVGA SGGTQITTSV ALAIINSLWF GYDVKRAVEE
510 520 530 540 550
PRLHNQLLPN TTTVEKNIDQ VVTAGLKTRH HHTEVTPDFI AVVQAVVRTS
560
GGWAAASDSR KGGEPAGY
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketM0R8W8 | M0R8W8_RAT | Glutathione hydrolase 1 proenzyme | Ggt1 | 447 | Annotation score: | ||
A0A1W2Q6L5 | A0A1W2Q6L5_RAT | Glutathione hydrolase 1 proenzyme | Ggt1 | 326 | Annotation score: | ||
A0A1W2Q6N5 | A0A1W2Q6N5_RAT | Glutathione hydrolase 1 proenzyme | Ggt1 | 48 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 9 | G → A in AAA41217 (PubMed:1671556).Curated | 1 | |
Sequence conflicti | 39 | R → K in AAA57295 (PubMed:2869484).Curated | 1 | |
Sequence conflicti | 39 | R → K in AAB59698 (PubMed:2869484).Curated | 1 | |
Sequence conflicti | 111 | R → K in AAB59698 (PubMed:2869484).Curated | 1 | |
Sequence conflicti | 370 | P → A in AAA57295 (PubMed:2869484).Curated | 1 | |
Sequence conflicti | 370 | P → A in AAB59698 (PubMed:2869484).Curated | 1 | |
Sequence conflicti | 370 | P → A in CAA27224 (PubMed:2869471).Curated | 1 | |
Sequence conflicti | 397 | S → M in CAA27224 (PubMed:2869471).Curated | 1 | |
Sequence conflicti | 416 | F → V in CAA27224 (PubMed:2869471).Curated | 1 | |
Sequence conflicti | 444 | P → L in AAA57295 (PubMed:2869484).Curated | 1 | |
Sequence conflicti | 444 | P → L in AAB59698 (PubMed:2869484).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X15443 mRNA Translation: CAA33483.1 M33821 mRNA Translation: AAA57295.1 Sequence problems. M33822 mRNA Translation: AAB59698.1 BC078768 mRNA Translation: AAH78768.1 L29167 mRNA Translation: AAA41218.1 M57672 Genomic DNA Translation: AAA41217.1 X03518 mRNA Translation: CAA27224.1 Frameshift. |
PIRi | A05225 |
RefSeqi | NP_446292.2, NM_053840.2 |
Genome annotation databases
GeneIDi | 116568 |
KEGGi | rno:116568 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X15443 mRNA Translation: CAA33483.1 M33821 mRNA Translation: AAA57295.1 Sequence problems. M33822 mRNA Translation: AAB59698.1 BC078768 mRNA Translation: AAH78768.1 L29167 mRNA Translation: AAA41218.1 M57672 Genomic DNA Translation: AAA41217.1 X03518 mRNA Translation: CAA27224.1 Frameshift. |
PIRi | A05225 |
RefSeqi | NP_446292.2, NM_053840.2 |
3D structure databases
SMRi | P07314 |
ModBasei | Search... |
Chemistry databases
BindingDBi | P07314 |
ChEMBLi | CHEMBL2943 |
DrugCentrali | P07314 |
PTM databases
GlyConnecti | 170, 1 O-Linked glycan |
GlyGeni | P07314, 8 sites, 2 N-linked glycans (1 site), 2 O-linked glycans (1 site) |
iPTMneti | P07314 |
PhosphoSitePlusi | P07314 |
Proteomic databases
PRIDEi | P07314 |
Genome annotation databases
GeneIDi | 116568 |
KEGGi | rno:116568 |
Organism-specific databases
CTDi | 2678 |
RGDi | 2683, Ggt1 |
Phylogenomic databases
InParanoidi | P07314 |
OMAi | CYSIDAR |
OrthoDBi | 1419292at2759 |
PhylomeDBi | P07314 |
Enzyme and pathway databases
UniPathwayi | UPA00204 |
BRENDAi | 2.3.2.2, 5301 |
Reactomei | R-RNO-174403, Glutathione synthesis and recycling R-RNO-2142691, Synthesis of Leukotrienes (LT) and Eoxins (EX) R-RNO-5423646, Aflatoxin activation and detoxification |
Miscellaneous databases
PROi | PR:P07314 |
Family and domain databases
Gene3Di | 1.10.246.130, 1 hit 3.60.20.40, 1 hit |
InterProi | View protein in InterPro IPR043138, GGT_lsub_C IPR000101, GGT_peptidase IPR043137, GGT_ssub IPR029055, Ntn_hydrolases_N |
PANTHERi | PTHR11686, PTHR11686, 1 hit |
SUPFAMi | SSF56235, SSF56235, 1 hit |
TIGRFAMsi | TIGR00066, g_glut_trans, 1 hit |
PROSITEi | View protein in PROSITE PS00462, G_GLU_TRANSPEPTIDASE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GGT1_RAT | |
Accessioni | P07314Primary (citable) accession number: P07314 Secondary accession number(s): Q63217, Q63218, Q6AZ32 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1988 |
Last sequence update: | May 1, 2007 | |
Last modified: | February 23, 2022 | |
This is version 168 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families