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UniProtKB - P07313 (MYLK2_RABIT)
Protein
Myosin light chain kinase 2, skeletal/cardiac muscle
Gene
MYLK2
Organism
Oryctolagus cuniculus (Rabbit)
Status
Functioni
Implicated in the level of global muscle contraction and cardiac function (By similarity).
Phosphorylates a specific serine in the N-terminus of a myosin light chain.
By similarityCatalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 326 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 418 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 303 – 311 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- calmodulin binding Source: BHF-UCL
- calmodulin-dependent protein kinase activity Source: BHF-UCL
- myosin light chain kinase activity Source: BHF-UCL
GO - Biological processi
- regulation of muscle filament sliding Source: BHF-UCL
Keywordsi
Molecular function | Calmodulin-binding, Kinase, Serine/threonine-protein kinase, Transferase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.11.18, 1749 |
Names & Taxonomyi
Protein namesi | Recommended name: Myosin light chain kinase 2, skeletal/cardiac muscle (EC:2.7.11.18)Short name: MLCK2 |
Gene namesi | Name:MYLK2 |
Organismi | Oryctolagus cuniculus (Rabbit) |
Taxonomic identifieri | 9986 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Note: Colocalizes with phosphorylated myosin light chain (RLCP) at filaments of the myofibrils.
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000086410 | 2 – 608 | Myosin light chain kinase 2, skeletal/cardiac muscleAdd BLAST | 607 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanine1 Publication | 1 | |
Modified residuei | 153 | PhosphoserineBy similarity | 1 | |
Modified residuei | 159 | PhosphoserineBy similarity | 1 | |
Modified residuei | 161 | PhosphoserineBy similarity | 1 | |
Modified residuei | 457 | PhosphothreonineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
PRIDEi | P07313 |
PTM databases
iPTMneti | P07313 |
Interactioni
Subunit structurei
May interact with centrin.
By similarityGO - Molecular functioni
- calmodulin binding Source: BHF-UCL
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P07313 |
BMRBi | P07313 |
SMRi | P07313 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P07313 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 297 – 552 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 256 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 160 | DisorderedSequence analysisAdd BLAST | 160 | |
Regioni | 214 – 235 | DisorderedSequence analysisAdd BLAST | 22 | |
Regioni | 586 – 598 | Calmodulin-bindingAdd BLAST | 13 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 9 – 23 | Polar residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 33 – 58 | Basic and acidic residuesSequence analysisAdd BLAST | 26 |
Sequence similaritiesi
Phylogenomic databases
InParanoidi | P07313 |
OrthoDBi | 218222at2759 |
Family and domain databases
CDDi | cd14190, STKc_MLCK2, 1 hit |
IDEALi | IID50197 |
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR042717, MLCK2_STKc IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P07313-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MATENGAVEL GIQSLSTDEA SKGAASEESL AAEKDPAPPD PEKGPGPSDT
60 70 80 90 100
KQDPDPSTPK KDANTPAPEK GDVVPAQPSA GGSQGPAGEG GQVEAPAEGS
110 120 130 140 150
AGKPAALPQQ TATAEASEKK PEAEKGPSGH QDPGEPTVGK KVAEGQAAAR
160 170 180 190 200
RGSPAFLHSP SCPAIIASTE KLPAQKPLSE ASELIFEGVP ATPGPTEPGP
210 220 230 240 250
AKAEGGVDLL AESQKEAGEK APGQADQAKV QGDTSRGIEF QAVPSERPRP
260 270 280 290 300
EVGQALCLPA REEDCFQILD DCPPPPAPFP HRIVELRTGN VSSEFSMNSK
310 320 330 340 350
EALGGGKFGA VCTCTEKSTG LKLAAKVIKK QTPKDKEMVM LEIEVMNQLN
360 370 380 390 400
HRNLIQLYAA IETPHEIVLF MEYIEGGELF ERIVDEDYHL TEVDTMVFVR
410 420 430 440 450
QICDGILFMH KMRVLHLDLK PENILCVNTT GHLVKIIDFG LARRYNPNEK
460 470 480 490 500
LKVNFGTPEF LSPEVVNYDQ ISDKTDMWSL GVITYMLLSG LSPFLGDDDT
510 520 530 540 550
ETLNNVLSGN WYFDEETFEA VSDEAKDFVS NLIVKEQGAR MSAAQCLAHP
560 570 580 590 600
WLNNLAEKAK RCNRRLKSQI LLKKYLMKRR WKKNFIAVSA ANRFKKISSS
GALMALGV
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 336 | K → KK AA sequence (PubMed:3542042).Curated | 1 | |
Sequence conflicti | 336 | K → KK AA sequence (PubMed:3841288).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J05194 mRNA Translation: AAA31400.1 |
PIRi | A35021 |
RefSeqi | NP_001075705.1, NM_001082236.1 |
Genome annotation databases
GeneIDi | 100009051 |
KEGGi | ocu:100009051 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J05194 mRNA Translation: AAA31400.1 |
PIRi | A35021 |
RefSeqi | NP_001075705.1, NM_001082236.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2BBM | NMR | - | B | 578-603 | [»] | |
2BBN | NMR | - | B | 578-603 | [»] | |
6HR1 | X-ray | 1.90 | A/B | 580-599 | [»] | |
AlphaFoldDBi | P07313 | |||||
BMRBi | P07313 | |||||
SMRi | P07313 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
PTM databases
iPTMneti | P07313 |
Proteomic databases
PRIDEi | P07313 |
Genome annotation databases
GeneIDi | 100009051 |
KEGGi | ocu:100009051 |
Organism-specific databases
CTDi | 85366 |
Phylogenomic databases
InParanoidi | P07313 |
OrthoDBi | 218222at2759 |
Enzyme and pathway databases
BRENDAi | 2.7.11.18, 1749 |
Miscellaneous databases
EvolutionaryTracei | P07313 |
Family and domain databases
CDDi | cd14190, STKc_MLCK2, 1 hit |
IDEALi | IID50197 |
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR042717, MLCK2_STKc IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MYLK2_RABIT | |
Accessioni | P07313Primary (citable) accession number: P07313 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1988 |
Last sequence update: | January 23, 2007 | |
Last modified: | May 25, 2022 | |
This is version 150 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families