UniProtKB - P07302 (CAPSD_MUMIM)
Protein
Capsid protein VP1
Gene
N/A
Organism
Murine minute virus (strain MVMi) (MVM) (Murine parvovirus)
Status
Functioni
Capsid protein self-assembles to form an icosahedral capsid with a T=1 symmetry, about 22 nm in diameter, and consisting of 60 copies of two size variants of the capsid proteins, VP1 and VP2, which differ by the presence of an N-terminal extension in the minor protein VP1. The capsid encapsulates the genomic ssDNA. Capsid proteins are responsible for the attachment to host cell receptors. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis. Binding to the host receptors also induces capsid rearrangements leading to surface exposure of VP1 N-terminus, specifically its phospholipase A2-like region and putative nuclear localization signal(s). VP1 N-terminus might serve as a lipolytic enzyme to breach the endosomal membrane during entry into host cell and might contribute to virus transport to the nucleus (By similarity).By similarity
Miscellaneous
The capsids of autonomous parvoviruses expose a proportion of VP2 N-terminus and part of that sequence can be cleaved of to form VP3.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 325 | Magnesium 1By similarity | 1 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- structural molecule activity Source: InterPro
GO - Biological processi
- clathrin-dependent endocytosis of virus by host cell Source: UniProtKB-KW
- microtubule-dependent intracellular transport of viral material towards nucleus Source: UniProtKB-KW
- permeabilization of host organelle membrane involved in viral entry into host cell Source: UniProtKB-KW
- viral entry via permeabilization of inner membrane Source: UniProtKB-KW
- viral penetration into host nucleus Source: UniProtKB-KW
- virion attachment to host cell Source: UniProtKB-KW
Keywordsi
Names & Taxonomyi
Protein namesi | Recommended name: Capsid protein VP1Alternative name(s): Coat protein VP1 |
Organismi | Murine minute virus (strain MVMi) (MVM) (Murine parvovirus) |
Taxonomic identifieri | 10795 [NCBI] |
Taxonomic lineagei | Viruses › Monodnaviria › Shotokuvirae › Cossaviricota › Quintoviricetes › Piccovirales › Parvoviridae › Parvovirinae › Protoparvovirus › |
Virus hosti | Mus musculus (Mouse) [TaxID: 10090] |
Proteomesi |
|
Subcellular locationi
- Virion By similarity
- Host nucleus Curated
GO - Cellular componenti
- host cell nucleus Source: UniProtKB-SubCell
- T=1 icosahedral viral capsid Source: UniProtKB-KW
Keywords - Cellular componenti
Capsid protein, Host nucleus, T=1 icosahedral capsid protein, VirionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000039417 | 1 – 729 | Capsid protein VP1Add BLAST | 729 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P07302 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P07302 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 19 – 64 | Phospholipase A2-likeBy similarityAdd BLAST | 46 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 4 – 13 | Nuclear localization signalSequence analysis | 10 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 167 – 183 | Gly-richAdd BLAST | 17 |
Domaini
The N-terminus of VP1 is sequestered within the mature capsid. It contains a phospholipase A2-like region and putative nuclear localization signals.
Sequence similaritiesi
Belongs to the parvoviridae capsid protein family.Curated
Family and domain databases
Gene3Di | 2.170.30.10, 1 hit |
InterProi | View protein in InterPro IPR016184, Capsid/spike_ssDNA_virus IPR001403, Parvovirus_coat IPR013607, Parvovirus_coat_VP1_N IPR036952, VP1/VP2 |
Pfami | View protein in Pfam PF00740, Parvo_coat, 1 hit PF08398, Parvo_coat_N, 1 hit |
SUPFAMi | SSF88645, SSF88645, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform VP1 (identifier: P07302-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAPPAKRAKR GWVPPGYKYL GPGNSLDQGE PTNPSDAAAK EHDEAYDQYI
60 70 80 90 100
KSGKNPYLYF SAADQRFIDQ TKDAKDWGGK VGHYFFRTKR AFAPKLATDS
110 120 130 140 150
EPGTSGVSRA GKRTRPPAYI FINQARAKKK LTSSAAQQSS QTMSDGTSQP
160 170 180 190 200
DGGNAVHSAA RVERAADGPG GSGGGGSGGG GVGVSTGSYD NQTHYRFLGD
210 220 230 240 250
GWVEITALAT RLVHLNMPKS ENYCRIRVHN TTDTSVKGNM AKDDAHEQIW
260 270 280 290 300
TPWSLVDANA WGVWLQPSDW QYICNTMSQL NLVSLDQEIF NVVLKTVTEQ
310 320 330 340 350
DSGGQAIKIY NNDLTACMMV AVDSNNILPY TPAANSMETL GFYPWKPTIA
360 370 380 390 400
SPYRYYFCVD RDLSVTYENQ EGTIEHNVMG TPKGMNSQFF TIENTQQITL
410 420 430 440 450
LRTGDEFATG TYYFDTNPVK LTHTWQTNRQ LGQPPLLSTF PEADTDAGTL
460 470 480 490 500
TAQGSRHGAT QMEVNWVSEA IRTRPAQVGF CQPHNDFEAS RAGPFAAPKV
510 520 530 540 550
PADVTQGVDR EANGSVRYSY GKQHGENWAA HGPAPERYTW DETNFGSGRD
560 570 580 590 600
TRDGFIQSAP LVVPPPLNGI LTNANPIGTK NDIHFSNVFN SYGPLTAFSH
610 620 630 640 650
PSPVYPQGQI WDKELDLEHK PRLHITAPFV CKNNAPGQML VRLGPNLTDQ
660 670 680 690 700
YDPNGATLSR IVTYGTFFWK GKLTMRAKLR ANTTWNPVYQ VSVEDNGNSY
710 720
MSVTKWLPTA TGNMQSVPLI TRPVARNTY
Note: Minor splicing isoform.
Isoform VP2 (identifier: P07302-2) [UniParc]FASTAAdd to basket
The sequence of this isoform differs from the canonical sequence as follows:
1-142: Missing.
Note: Major splicing isoform (approximately 70% of the molecules), produced by deletion of the initiating AUG for VP1 and downstream translation of VP2.Curated
Show »Sequence cautioni
The sequence AAA69569 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB46507 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB46508 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 155 | A → G in CAB46507 (PubMed:3855242).Curated | 1 | |
Sequence conflicti | 155 | A → G in CAB46508 (PubMed:3855242).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_041134 | 1 – 142 | Missing in isoform VP2. CuratedAdd BLAST | 142 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X02481 Genomic DNA Translation: CAB46507.1 Sequence problems. X02481 Genomic DNA Translation: CAB46508.1 Different initiation. M12032 Genomic DNA Translation: AAA69569.1 Sequence problems. |
PIRi | B23008, VCPVIM |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Web resourcesi
Virus Particle ExploreR db Icosahedral capsid structure |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X02481 Genomic DNA Translation: CAB46507.1 Sequence problems. X02481 Genomic DNA Translation: CAB46508.1 Different initiation. M12032 Genomic DNA Translation: AAA69569.1 Sequence problems. |
PIRi | B23008, VCPVIM |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1MVM | X-ray | 3.50 | A | 143-729 | [»] | |
1Z1C | X-ray | 3.50 | A | 143-729 | [»] | |
2XGK | X-ray | 4.20 | A | 143-729 | [»] | |
SMRi | P07302 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P07302 |
Family and domain databases
Gene3Di | 2.170.30.10, 1 hit |
InterProi | View protein in InterPro IPR016184, Capsid/spike_ssDNA_virus IPR001403, Parvovirus_coat IPR013607, Parvovirus_coat_VP1_N IPR036952, VP1/VP2 |
Pfami | View protein in Pfam PF00740, Parvo_coat, 1 hit PF08398, Parvo_coat_N, 1 hit |
SUPFAMi | SSF88645, SSF88645, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CAPSD_MUMIM | |
Accessioni | P07302Primary (citable) accession number: P07302 Secondary accession number(s): Q9WMH2, Q9WMH3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1988 |
Last sequence update: | May 31, 2011 | |
Last modified: | February 10, 2021 | |
This is version 113 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structureDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families