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Protein

UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase

Gene

ALG7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the initial step in the synthesis of dolichol-P-P-oligosaccharides.

Catalytic activityi

UDP-N-acetyl-D-glucosamine + dolichyl phosphate = UMP + N-acetyl-D-glucosaminyl-diphosphodolichol.

Activity regulationi

Inhibited by tunicamycin.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciMetaCyc:YBR243C-MONOMER
YEAST:YBR243C-MONOMER
ReactomeiR-SCE-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
UniPathwayi
UPA00378

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase (EC:2.7.8.15)
Alternative name(s):
GlcNAc-1-P transferase
Short name:
G1PT
Short name:
GPT
N-acetylglucosamine-1-phosphate transferase
Tunicamycin resistance protein 1
Gene namesi
Name:ALG7
Synonyms:TUR1
Ordered Locus Names:YBR243C
ORF Names:YBR1628
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR243C
SGDiS000000447 ALG7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei24 – 44HelicalSequence analysisAdd BLAST21
Transmembranei72 – 92HelicalSequence analysisAdd BLAST21
Transmembranei129 – 149HelicalSequence analysisAdd BLAST21
Transmembranei157 – 177HelicalSequence analysisAdd BLAST21
Transmembranei202 – 222HelicalSequence analysisAdd BLAST21
Transmembranei231 – 251HelicalSequence analysisAdd BLAST21
Transmembranei256 – 276HelicalSequence analysisAdd BLAST21
Transmembranei283 – 303HelicalSequence analysisAdd BLAST21
Transmembranei309 – 329HelicalSequence analysisAdd BLAST21
Transmembranei387 – 407HelicalSequence analysisAdd BLAST21
Transmembranei419 – 439HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001087651 – 448UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferaseAdd BLAST448

Proteomic databases

MaxQBiP07286
PaxDbiP07286
PRIDEiP07286

Interactioni

Protein-protein interaction databases

BioGridi32938, 44 interactors
DIPiDIP-5364N
IntActiP07286, 7 interactors
MINTiP07286
STRINGi4932.YBR243C

Structurei

3D structure databases

ProteinModelPortaliP07286
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi81 – 93Dolichol recognitionAdd BLAST13
Motifi257 – 269Dolichol recognitionAdd BLAST13

Sequence similaritiesi

Belongs to the glycosyltransferase 4 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000011424
HOGENOMiHOG000163915
InParanoidiP07286
KOiK01001
OMAiGDYRDDH
OrthoDBiEOG092C2SKV

Family and domain databases

CDDicd06855 GT_GPT_euk, 1 hit
InterProiView protein in InterPro
IPR000715 Glycosyl_transferase_4
IPR033895 GPT
PANTHERiPTHR10571 PTHR10571, 1 hit
PfamiView protein in Pfam
PF00953 Glycos_transf_4, 1 hit

Sequencei

Sequence statusi: Complete.

P07286-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLRLFSLALI TCLIYYSKNQ GPSALVAAVG FGIAGYLATD MLIPRVGKSF
60 70 80 90 100
IKIGLFGKDL SKPGRPVLPE TIGAIPAAVY LFVMFIYIPF IFYKYMVITT
110 120 130 140 150
SGGGHRDVSV VEDNGMNSNI FPHDKLSEYL SAILCLESTV LLGIADDLFD
160 170 180 190 200
LRWRHKFFLP AIAAIPLLMV YYVDFGVTHV LIPGFMERWL KKTSVDLGLW
210 220 230 240 250
YYVYMASMAI FCPNSINILA GVNGLEVGQC IVLAILALLN DLLYFSMGPL
260 270 280 290 300
ATRDSHRFSA VLIIPFLGVS LALWKWNRWP ATVFVGDTYC YFAGMVFAVV
310 320 330 340 350
GILGHFSKTM LLLFIPQIVN FIYSCPQLFK LVPCPRHRLP KFNEKDGLMY
360 370 380 390 400
PSRANLKEEP PKSIFKPILK LLYCLHLIDL EFDENNEIIS TSNMTLINLT
410 420 430 440
LVWFGPMRED KLCNTILKLQ FCIGILALLG RHAIGAIIFG HDNLWTVR
Length:448
Mass (Da):50,368
Last modified:April 1, 1988 - v1
Checksum:i00A617D831335637
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00126 Genomic DNA Translation: CAA68324.1
Z36112 Genomic DNA Translation: CAA85206.1
BK006936 Genomic DNA Translation: DAA07359.1
PIRiA27522
RefSeqiNP_009802.3, NM_001178591.3

Genome annotation databases

EnsemblFungiiYBR243C; YBR243C; YBR243C
GeneIDi852545
KEGGisce:YBR243C

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00126 Genomic DNA Translation: CAA68324.1
Z36112 Genomic DNA Translation: CAA85206.1
BK006936 Genomic DNA Translation: DAA07359.1
PIRiA27522
RefSeqiNP_009802.3, NM_001178591.3

3D structure databases

ProteinModelPortaliP07286
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32938, 44 interactors
DIPiDIP-5364N
IntActiP07286, 7 interactors
MINTiP07286
STRINGi4932.YBR243C

Proteomic databases

MaxQBiP07286
PaxDbiP07286
PRIDEiP07286

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR243C; YBR243C; YBR243C
GeneIDi852545
KEGGisce:YBR243C

Organism-specific databases

EuPathDBiFungiDB:YBR243C
SGDiS000000447 ALG7

Phylogenomic databases

GeneTreeiENSGT00390000011424
HOGENOMiHOG000163915
InParanoidiP07286
KOiK01001
OMAiGDYRDDH
OrthoDBiEOG092C2SKV

Enzyme and pathway databases

UniPathwayi
UPA00378

BioCyciMetaCyc:YBR243C-MONOMER
YEAST:YBR243C-MONOMER
ReactomeiR-SCE-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein

Miscellaneous databases

PROiPR:P07286

Family and domain databases

CDDicd06855 GT_GPT_euk, 1 hit
InterProiView protein in InterPro
IPR000715 Glycosyl_transferase_4
IPR033895 GPT
PANTHERiPTHR10571 PTHR10571, 1 hit
PfamiView protein in Pfam
PF00953 Glycos_transf_4, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGPT_YEAST
AccessioniPrimary (citable) accession number: P07286
Secondary accession number(s): D6VQN9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: October 10, 2018
This is version 155 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names
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Main funding by: National Institutes of Health

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