UniProtKB - P07275 (PUT2_YEAST)
Protein
Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial
Gene
PUT2
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Miscellaneous
Present with 17200 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- EC:1.2.1.88
: L-proline degradation into L-glutamate Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes L-glutamate from L-proline.Proteins known to be involved in the 2 steps of the subpathway in this organism are:
- Proline dehydrogenase, mitochondrial (PUT1)
- Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial (PUT2)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate from L-proline, the pathway L-proline degradation into L-glutamate and in Amino-acid degradation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 212 | Transition state stabilizerBy similarity | 1 | |
Active sitei | 317 | Proton acceptorPROSITE-ProRule annotation | 1 | |
Active sitei | 351 | NucleophilePROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 297 – 302 | NADBy similarity | 6 |
GO - Molecular functioni
- 1-pyrroline-5-carboxylate dehydrogenase activity Source: SGD
- oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor Source: InterPro
GO - Biological processi
- glutamate biosynthetic process Source: SGD
- proline catabolic process to glutamate Source: SGD
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Proline metabolism |
Ligand | NAD |
Enzyme and pathway databases
BioCyci | MetaCyc:YHR037W-MONOMER YEAST:YHR037W-MONOMER |
UniPathwayi | UPA00261;UER00374 |
Names & Taxonomyi
Protein namesi | Recommended name: Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial (EC:1.2.1.88)Short name: P5C dehydrogenase Alternative name(s): L-glutamate gamma-semialdehyde dehydrogenase |
Gene namesi | Name:PUT2 Ordered Locus Names:YHR037W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000001079, PUT2 |
VEuPathDBi | FungiDB:YHR037W |
Subcellular locationi
Mitochondrion
- Mitochondrion inner membrane 1 Publication
Mitochondrion
- mitochondrial inner membrane Source: UniProtKB-SubCell
- mitochondrial matrix Source: SGD
- mitochondrion Source: SGD
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion inner membranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – ? | MitochondrionSequence analysis | ||
ChainiPRO_0000007177 | ? – 575 | Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial |
Proteomic databases
MaxQBi | P07275 |
PaxDbi | P07275 |
PRIDEi | P07275 |
Expressioni
Inductioni
By proline and is regulated by a common control element encoded by the PUT3 gene.
Interactioni
Protein-protein interaction databases
BioGRIDi | 36468, 112 interactors |
IntActi | P07275, 3 interactors |
STRINGi | 4932.YHR037W |
Miscellaneous databases
RNActi | P07275, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P07275 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the aldehyde dehydrogenase family.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG2455, Eukaryota |
GeneTreei | ENSGT00560000077335 |
HOGENOMi | CLU_005391_4_1_1 |
InParanoidi | P07275 |
OMAi | FAGIHFT |
Family and domain databases
Gene3Di | 3.40.309.10, 1 hit 3.40.605.10, 1 hit |
InterProi | View protein in InterPro IPR016161, Ald_DH/histidinol_DH IPR016163, Ald_DH_C IPR016160, Ald_DH_CS_CYS IPR029510, Ald_DH_CS_GLU IPR016162, Ald_DH_N IPR015590, Aldehyde_DH_dom IPR005931, P5CDH/ALDH4A1 |
Pfami | View protein in Pfam PF00171, Aldedh, 1 hit |
SUPFAMi | SSF53720, SSF53720, 1 hit |
TIGRFAMsi | TIGR01236, D1pyr5carbox1, 1 hit |
PROSITEi | View protein in PROSITE PS00070, ALDEHYDE_DEHYDR_CYS, 1 hit PS00687, ALDEHYDE_DEHYDR_GLU, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P07275-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLSARCLKSI YFKRSFSQLG HIKPPKHIRN EPVKPFRNID LKDWDLLRAS
60 70 80 90 100
LMKFKSSSLE VPLVINGERI YDNNERALFP QTNPANHQQV LANVTQATEK
110 120 130 140 150
DVMNAVKAAK DAKKDWYNLP FYDRSAIFLK AADLISTKYR YDMLAATMLG
160 170 180 190 200
QGKNVYQAEI DCITELSDFF RYYVKYASDL YAQQPVESAD GTWNKAEYRP
210 220 230 240 250
LEGFVYAVSP FNFTAIAANL IGAPALMGNT VVWKPSQTAA LSNYLLMTVL
260 270 280 290 300
EEAGLPKGVI NFIPGDPVQV TDQVLADKDF GALHFTGSTN VFKSLYGKIQ
310 320 330 340 350
SGVVEGKYRD YPRIIGETGG KNFHLVHPSA NISHAVLSTI RGTFEFQGQK
360 370 380 390 400
CSAASRLYLP ESKSEEFLSD MFGILQSQNV VPMNTSASPI SGGNLRGFMG
410 420 430 440 450
PVIHEQSFDK LVKVIEDAKK DPELEILYGG QYDKSQGWFV GPTVIKAKRP
460 470 480 490 500
DHPYMSTEFF GPILTVYEYP DTEFNEICDI IDNTSQYALT GAIFAKDRKA
510 520 530 540 550
IEYADEKLKF SAGNFYINDK CTGAVVSQQW FGGARMSGTD DKAGGPNILS
560 570
RFVSIRNTKE NFYELTDFKY PSNYE
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 187 – 188 | ES → SR in AAA34924 (PubMed:6098824).Curated | 2 | |
Sequence conflicti | 264 | P → L in AAA34924 (PubMed:6098824).Curated | 1 | |
Sequence conflicti | 541 | D → G in AAA34924 (PubMed:6098824).Curated | 1 | |
Sequence conflicti | 561 | N → S in AAA34924 (PubMed:6098824).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M10029 Genomic DNA Translation: AAA34924.1 M22785 Genomic DNA No translation available. U00062 Genomic DNA Translation: AAB68907.1 BK006934 Genomic DNA Translation: DAA06728.1 |
PIRi | S46738, RDBYC |
RefSeqi | NP_011902.1, NM_001179167.1 |
Genome annotation databases
EnsemblFungii | YHR037W_mRNA; YHR037W; YHR037W |
GeneIDi | 856432 |
KEGGi | sce:YHR037W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M10029 Genomic DNA Translation: AAA34924.1 M22785 Genomic DNA No translation available. U00062 Genomic DNA Translation: AAB68907.1 BK006934 Genomic DNA Translation: DAA06728.1 |
PIRi | S46738, RDBYC |
RefSeqi | NP_011902.1, NM_001179167.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4OE4 | X-ray | 2.17 | A/B | 23-575 | [»] | |
4OE6 | X-ray | 1.95 | A/B | 23-575 | [»] | |
SMRi | P07275 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 36468, 112 interactors |
IntActi | P07275, 3 interactors |
STRINGi | 4932.YHR037W |
Proteomic databases
MaxQBi | P07275 |
PaxDbi | P07275 |
PRIDEi | P07275 |
Genome annotation databases
EnsemblFungii | YHR037W_mRNA; YHR037W; YHR037W |
GeneIDi | 856432 |
KEGGi | sce:YHR037W |
Organism-specific databases
SGDi | S000001079, PUT2 |
VEuPathDBi | FungiDB:YHR037W |
Phylogenomic databases
eggNOGi | KOG2455, Eukaryota |
GeneTreei | ENSGT00560000077335 |
HOGENOMi | CLU_005391_4_1_1 |
InParanoidi | P07275 |
OMAi | FAGIHFT |
Enzyme and pathway databases
UniPathwayi | UPA00261;UER00374 |
BioCyci | MetaCyc:YHR037W-MONOMER YEAST:YHR037W-MONOMER |
Miscellaneous databases
PROi | PR:P07275 |
RNActi | P07275, protein |
Family and domain databases
Gene3Di | 3.40.309.10, 1 hit 3.40.605.10, 1 hit |
InterProi | View protein in InterPro IPR016161, Ald_DH/histidinol_DH IPR016163, Ald_DH_C IPR016160, Ald_DH_CS_CYS IPR029510, Ald_DH_CS_GLU IPR016162, Ald_DH_N IPR015590, Aldehyde_DH_dom IPR005931, P5CDH/ALDH4A1 |
Pfami | View protein in Pfam PF00171, Aldedh, 1 hit |
SUPFAMi | SSF53720, SSF53720, 1 hit |
TIGRFAMsi | TIGR01236, D1pyr5carbox1, 1 hit |
PROSITEi | View protein in PROSITE PS00070, ALDEHYDE_DEHYDR_CYS, 1 hit PS00687, ALDEHYDE_DEHYDR_GLU, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PUT2_YEAST | |
Accessioni | P07275Primary (citable) accession number: P07275 Secondary accession number(s): D3DKY4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1988 |
Last sequence update: | February 1, 1995 | |
Last modified: | April 7, 2021 | |
This is version 194 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome VIII
Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families