DST1

Functionⁱ

Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.

Can promote the transfer of one strand of a double-stranded DNA molecule to a homologous single strand and thus may be involved in recombination.

Miscellaneous

S-II binds to RNA-polymerase II in the absence of transcription.

Present with 6260 molecules/cell in log phase SD medium.1 Publication

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Zinc fingerⁱ	267 – 307	TFIIS-typePROSITE-ProRule annotationAdd BLAST		41

GO - Molecular functionⁱ

Complete GO annotation on QuickGO ...

GO - Biological processⁱ

Complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	DNA-binding
Biological process	Transcription, Transcription regulation
Ligand	Metal-binding, Zinc

Enzyme and pathway databases

Reactomeⁱ	R-SCE-674695, RNA Polymerase II Pre-transcription Events R-SCE-6782135, Dual incision in TC-NER R-SCE-6782210, Gap-filling DNA repair synthesis and ligation in TC-NER R-SCE-6796648, TP53 Regulates Transcription of DNA Repair Genes R-SCE-75955, RNA Polymerase II Transcription Elongation

Reactomeⁱ

R-SCE-674695, RNA Polymerase II Pre-transcription Events
R-SCE-6782135, Dual incision in TC-NER
R-SCE-6782210, Gap-filling DNA repair synthesis and ligation in TC-NER
R-SCE-6796648, TP53 Regulates Transcription of DNA Repair Genes
R-SCE-75955, RNA Polymerase II Transcription Elongation

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Transcription elongation factor S-II Alternative name(s): DNA strand transfer protein alpha Short name: STP-alpha DNA strand transferase 1 Pyrimidine pathway regulatory protein 2
Gene namesⁱ	Name:DST1 Synonyms:PPR2 Ordered Locus Names:YGL043W
Organismⁱ	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifierⁱ	559292 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae
Proteomesⁱ	UP000002311 Componentⁱ: Chromosome VII

Organism-specific databases

Saccharomyces Genome Database More...SGDⁱ	S000003011, DST1
VEuPathDBⁱ	FungiDB:YGL043W

Keywords - Cellular componentⁱ

Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000121443	1 – 309	Transcription elongation factor S-IIAdd BLAST		309

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	116	PhosphoserineCombined sources		1

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P07273
PaxDbⁱ	P07273
PRIDEⁱ	P07273

PTM databases

iPTMnetⁱ	P07273

iPTMnetⁱ

P07273

Interactionⁱ

Binary interactionsⁱ

Hide details

P07273

With	#Exp.	IntAct
SPT8 [P38915]	2	EBI-19168,EBI-17964
SSN2 [P38931]	2	EBI-19168,EBI-18059

GO - Molecular functionⁱ

Complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGRIDⁱ	33205, 589 interactors
Database of interacting proteins More...DIPⁱ	DIP-2307N
IntActⁱ	P07273, 7 interactors
Molecular INTeraction database More...MINTⁱ	P07273
STRINGⁱ	4932.YGL043W

Miscellaneous databases

RNActⁱ	P07273, protein

RNActⁱ

P07273, protein

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	3 – 16	Combined sources	14
Beta strandⁱ	18 – 20	Combined sources	3
Helixⁱ	21 – 30	Combined sources	10
Turnⁱ	31 – 33	Combined sources	3
Helixⁱ	40 – 52	Combined sources	13
Beta strandⁱ	55 – 57	Combined sources	3
Helixⁱ	61 – 76	Combined sources	16
Turnⁱ	134 – 137	Combined sources	4
Helixⁱ	147 – 159	Combined sources	13
Beta strandⁱ	162 – 165	Combined sources	4
Helixⁱ	170 – 185	Combined sources	16
Beta strandⁱ	189 – 192	Combined sources	4
Helixⁱ	194 – 207	Combined sources	14
Beta strandⁱ	208 – 210	Combined sources	3
Helixⁱ	213 – 220	Combined sources	8
Helixⁱ	226 – 229	Combined sources	4
Turnⁱ	232 – 235	Combined sources	4
Helixⁱ	240 – 255	Combined sources	16
Beta strandⁱ	266 – 269	Combined sources	4
Beta strandⁱ	272 – 274	Combined sources	3
Beta strandⁱ	297 – 299	Combined sources	3
Turnⁱ	300 – 302	Combined sources	3

Show more details

3D structure databases

BMRBⁱ	P07273
SMRⁱ	P07273
ModBaseⁱ	Search...
PDBe-KBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ

P07273

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	5 – 79	TFIIS N-terminalPROSITE-ProRule annotationAdd BLAST		75
Domainⁱ	148 – 264	TFIIS centralPROSITE-ProRule annotationAdd BLAST		117

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	78 – 142	DisorderedSequence analysisAdd BLAST		65

Compositional bias

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Compositional biasⁱ	78 – 98	Basic and acidic residuesSequence analysisAdd BLAST		21
Compositional biasⁱ	101 – 124	Polar residuesSequence analysisAdd BLAST		24

Sequence similaritiesⁱ

Belongs to the TFS-II family.Curated

Zinc finger

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Zinc fingerⁱ	267 – 307	TFIIS-typePROSITE-ProRule annotationAdd BLAST		41

Keywords - Domainⁱ

Zinc-finger

Phylogenomic databases

eggNOGⁱ	KOG1105, Eukaryota
HOGENOMⁱ	CLU_037637_1_0_1
InParanoidⁱ	P07273
OMAⁱ	TTFCECT

Family and domain databases

Gene3Dⁱ	1.10.472.30, 1 hit 1.20.930.10, 1 hit
InterProⁱ	View protein in InterPro IPR035100, TF_IIS-typ IPR003617, TFIIS/CRSP70_N_sub IPR035441, TFIIS/LEDGF_dom_sf IPR003618, TFIIS_cen_dom IPR036575, TFIIS_cen_dom_sf IPR017923, TFIIS_N IPR006289, TFSII IPR001222, Znf_TFIIS
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF08711, Med26, 1 hit PF01096, TFIIS_C, 1 hit PF07500, TFIIS_M, 1 hit
PIRSFⁱ	PIRSF006704, TF_IIS, 1 hit
SMARTⁱ	View protein in SMART SM00510, TFS2M, 1 hit SM00509, TFS2N, 1 hit SM00440, ZnF_C2C2, 1 hit
SUPFAMⁱ	SSF46942, SSF46942, 1 hit SSF47676, SSF47676, 1 hit
TIGRFAMsⁱ	TIGR01385, TFSII, 1 hit
PROSITEⁱ	View protein in PROSITE PS51321, TFIIS_CENTRAL, 1 hit PS51319, TFIIS_N, 1 hit PS00466, ZF_TFIIS_1, 1 hit PS51133, ZF_TFIIS_2, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P07273-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MDSKEVLVHV KNLEKNKSND AAVLEILHVL DKEFVPTEKL LRETKVGVEV 
        60         70         80         90        100
NKFKKSTNVE ISKLVKKMIS SWKDAINKNK RSRQAQQHHQ DHAPGNAEDK 
       110        120        130        140        150
TTVGESVNGV QQPASSQSDA MKQDKYVSTK PRNSKNDGVD TAIYHHKLRD 
       160        170        180        190        200
QVLKALYDVL AKESEHPPQS ILHTAKAIES EMNKVNNCDT NEAAYKARYR 
       210        220        230        240        250
IIYSNVISKN NPDLKHKIAN GDITPEFLAT CDAKDLAPAP LKQKIEEIAK 
       260        270        280        290        300
QNLYNAQGAT IERSVTDRFT CGKCKEKKVS YYQLQTRSAD EPLTTFCTCE 

ACGNRWKFS

Length:309

Mass (Da):34,843

Last modified:October 1, 1996 - v4

Checksum:ⁱD133938319C015FF

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	35	V → F in AAA88734 (PubMed:1850099).Curated	1
Sequence conflictⁱ	35	V → F in AAA34580 (PubMed:1850099).Curated	1
Sequence conflictⁱ	74 – 77	DAIN → AQLI in AAA88734 (PubMed:1850099).Curated	4
Sequence conflictⁱ	74 – 77	DAIN → AQLI in AAA34580 (PubMed:1850099).Curated	4
Sequence conflictⁱ	82	S → C in AAA88734 (PubMed:1850099).Curated	1
Sequence conflictⁱ	82	S → C in AAA34580 (PubMed:1850099).Curated	1
Sequence conflictⁱ	85	A → P in AAA88734 (PubMed:1850099).Curated	1
Sequence conflictⁱ	85	A → P in AAA34580 (PubMed:1850099).Curated	1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M36724 Genomic DNA Translation: AAA88734.1 D12478 Genomic DNA Translation: BAA02046.1 M60770 Genomic DNA Translation: AAA34580.1 Z72565 Genomic DNA Translation: CAA96744.1 X00047 Genomic DNA Translation: CAA24928.1 BK006941 Genomic DNA Translation: DAA08057.1
PIRⁱ	A42921
NCBI Reference Sequences More...RefSeqⁱ	NP_011472.1, NM_001180908.1

Genome annotation databases

EnsemblFungiⁱ	YGL043W_mRNA; YGL043W; YGL043W
GeneIDⁱ	852839
KEGGⁱ	sce:YGL043W

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P07273	BJ4_G0040560.mRNA.1.CDS.1		YEASX		309	UniRef100_P07273
DST1p General transcription elongation factor TFIIS		Saccharomyces boulardii (nom. inval.)		309
K7_Dst1p		YEASK		309
Dst1p		SACCK		309
Dst1p		YEASC		309
+8

Protein	Similar proteins		Species	Score	Length	Source
P07273	BJ4_G0040560.mRNA.1.CDS.1		YEASX		309	UniRef90_P07273
DST1p General transcription elongation factor TFIIS		Saccharomyces boulardii (nom. inval.)		309
K7_Dst1p		YEASK		309
Dst1p		SACCK		309
Dst1p		YEASC		309
+13

Protein	Similar proteins		Species	Score	Length	Source
P07273	BJ4_G0040560.mRNA.1.CDS.1		YEASX		309	UniRef50_P07273
DST1p General transcription elongation factor TFIIS		Saccharomyces boulardii (nom. inval.)		309
K7_Dst1p		YEASK		309
Dst1p		SACCK		309
Dst1p		YEASC		309
+55

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M36724 Genomic DNA Translation: AAA88734.1 D12478 Genomic DNA Translation: BAA02046.1 M60770 Genomic DNA Translation: AAA34580.1 Z72565 Genomic DNA Translation: CAA96744.1 X00047 Genomic DNA Translation: CAA24928.1 BK006941 Genomic DNA Translation: DAA08057.1
PIRⁱ	A42921
RefSeqⁱ	NP_011472.1, NM_001180908.1

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1ENW	NMR	-	A	131-240	[»]
	1EO0	NMR	-	A	1-77	[»]
	1PQV	X-ray	3.80	S	1-309	[»]
	1Y1V	X-ray	3.80	S	131-309	[»]
	1Y1Y	X-ray	4.00	S	131-309	[»]
	3GTM	X-ray	3.80	S	147-309	[»]
	3PO3	X-ray	3.30	S	132-309	[»]
	5FMF	electron microscopy	6.00	2	136-309	[»]
BMRBⁱ	P07273
SMRⁱ	P07273
ModBaseⁱ	Search...
PDBe-KBⁱ	Search...

Protein-protein interaction databases

BioGRIDⁱ	33205, 589 interactors
DIPⁱ	DIP-2307N
IntActⁱ	P07273, 7 interactors
MINTⁱ	P07273
STRINGⁱ	4932.YGL043W

PTM databases

iPTMnetⁱ	P07273

iPTMnetⁱ

P07273

Proteomic databases

MaxQBⁱ	P07273
PaxDbⁱ	P07273
PRIDEⁱ	P07273

Genome annotation databases

EnsemblFungiⁱ	YGL043W_mRNA; YGL043W; YGL043W
GeneIDⁱ	852839
KEGGⁱ	sce:YGL043W

Organism-specific databases

SGDⁱ	S000003011, DST1
VEuPathDBⁱ	FungiDB:YGL043W

Phylogenomic databases

eggNOGⁱ	KOG1105, Eukaryota
HOGENOMⁱ	CLU_037637_1_0_1
InParanoidⁱ	P07273
OMAⁱ	TTFCECT

Enzyme and pathway databases

Reactomeⁱ	R-SCE-674695, RNA Polymerase II Pre-transcription Events R-SCE-6782135, Dual incision in TC-NER R-SCE-6782210, Gap-filling DNA repair synthesis and ligation in TC-NER R-SCE-6796648, TP53 Regulates Transcription of DNA Repair Genes R-SCE-75955, RNA Polymerase II Transcription Elongation

Reactomeⁱ

R-SCE-674695, RNA Polymerase II Pre-transcription Events
R-SCE-6782135, Dual incision in TC-NER
R-SCE-6782210, Gap-filling DNA repair synthesis and ligation in TC-NER
R-SCE-6796648, TP53 Regulates Transcription of DNA Repair Genes
R-SCE-75955, RNA Polymerase II Transcription Elongation

Miscellaneous databases

EvolutionaryTraceⁱ	P07273
Protein Ontology More...PROⁱ	PR:P07273
RNActⁱ	P07273, protein

Family and domain databases

Gene3Dⁱ	1.10.472.30, 1 hit 1.20.930.10, 1 hit
InterProⁱ	View protein in InterPro IPR035100, TF_IIS-typ IPR003617, TFIIS/CRSP70_N_sub IPR035441, TFIIS/LEDGF_dom_sf IPR003618, TFIIS_cen_dom IPR036575, TFIIS_cen_dom_sf IPR017923, TFIIS_N IPR006289, TFSII IPR001222, Znf_TFIIS
Pfamⁱ	View protein in Pfam PF08711, Med26, 1 hit PF01096, TFIIS_C, 1 hit PF07500, TFIIS_M, 1 hit
PIRSFⁱ	PIRSF006704, TF_IIS, 1 hit
SMARTⁱ	View protein in SMART SM00510, TFS2M, 1 hit SM00509, TFS2N, 1 hit SM00440, ZnF_C2C2, 1 hit
SUPFAMⁱ	SSF46942, SSF46942, 1 hit SSF47676, SSF47676, 1 hit
TIGRFAMsⁱ	TIGR01385, TFSII, 1 hit
PROSITEⁱ	View protein in PROSITE PS51321, TFIIS_CENTRAL, 1 hit PS51319, TFIIS_N, 1 hit PS00466, ZF_TFIIS_1, 1 hit PS51133, ZF_TFIIS_2, 1 hit
MobiDBⁱ	Search...

Entry informationⁱ

Entry nameⁱ	TFS2_YEAST
Accessionⁱ	P07273Primary (citable) accession number: P07273 Secondary accession number(s): D6VU96, P24535
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
	Last sequence update:	October 1, 1996
	Last modified:	June 2, 2021
	This is version 212 of the entry and version 4 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Fungal Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Direct protein sequencing, Reference proteome

Documents

Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
Yeast chromosome VII
Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names
PDB cross-references
Index of Protein Data Bank (PDB) cross-references
SIMILARITY comments
Index of protein domains and families

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P07273 (TFS2_YEAST)

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Transcription elongation factor S-II

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Nucleus

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

P07273

GO - Molecular functioni

Protein-protein interaction databases

Miscellaneous databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

Compositional bias

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Zinc finger

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ