UniProtKB - P07273 (TFS2_YEAST)
Protein
Transcription elongation factor S-II
Gene
DST1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.
Can promote the transfer of one strand of a double-stranded DNA molecule to a homologous single strand and thus may be involved in recombination.
Miscellaneous
S-II binds to RNA-polymerase II in the absence of transcription.
Present with 6260 molecules/cell in log phase SD medium.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 267 – 307 | TFIIS-typePROSITE-ProRule annotationAdd BLAST | 41 |
GO - Molecular functioni
- RNA polymerase II complex binding Source: SGD
- RNA polymerase II complex recruiting activity Source: SGD
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: SGD
- zinc ion binding Source: InterPro
GO - Biological processi
- maintenance of transcriptional fidelity during DNA-templated transcription elongation from RNA polymerase II promoter Source: SGD
- negative regulation of transcription by RNA polymerase II Source: GO_Central
- positive regulation of RNA polymerase II transcription preinitiation complex assembly Source: SGD
- positive regulation of transcription elongation from RNA polymerase II promoter Source: SGD
- regulation of mRNA 3'-end processing Source: SGD
- transcription antitermination Source: SGD
- transcription by RNA polymerase III Source: SGD
- transcription elongation from RNA polymerase II promoter Source: SGD
- transcription elongation from RNA polymerase I promoter Source: SGD
- transcription initiation from RNA polymerase II promoter Source: SGD
- tRNA transcription by RNA polymerase III Source: SGD
Keywordsi
Molecular function | DNA-binding |
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-SCE-674695, RNA Polymerase II Pre-transcription Events R-SCE-6782135, Dual incision in TC-NER R-SCE-6782210, Gap-filling DNA repair synthesis and ligation in TC-NER R-SCE-6796648, TP53 Regulates Transcription of DNA Repair Genes R-SCE-75955, RNA Polymerase II Transcription Elongation |
Names & Taxonomyi
Protein namesi | Recommended name: Transcription elongation factor S-IIAlternative name(s): DNA strand transfer protein alpha Short name: STP-alpha DNA strand transferase 1 Pyrimidine pathway regulatory protein 2 |
Gene namesi | Name:DST1 Synonyms:PPR2 Ordered Locus Names:YGL043W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YGL043W |
SGDi | S000003011, DST1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000121443 | 1 – 309 | Transcription elongation factor S-IIAdd BLAST | 309 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 116 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P07273 |
PaxDbi | P07273 |
PRIDEi | P07273 |
PTM databases
iPTMneti | P07273 |
Interactioni
Binary interactionsi
Hide detailsGO - Molecular functioni
- RNA polymerase II complex binding Source: SGD
- RNA polymerase II complex recruiting activity Source: SGD
Protein-protein interaction databases
BioGRIDi | 33205, 587 interactors |
DIPi | DIP-2307N |
IntActi | P07273, 7 interactors |
MINTi | P07273 |
STRINGi | 4932.YGL043W |
Miscellaneous databases
RNActi | P07273, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P07273 |
SMRi | P07273 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P07273 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 5 – 79 | TFIIS N-terminalPROSITE-ProRule annotationAdd BLAST | 75 | |
Domaini | 148 – 264 | TFIIS centralPROSITE-ProRule annotationAdd BLAST | 117 |
Sequence similaritiesi
Belongs to the TFS-II family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 267 – 307 | TFIIS-typePROSITE-ProRule annotationAdd BLAST | 41 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG1105, Eukaryota |
GeneTreei | ENSGT00960000189196 |
HOGENOMi | CLU_037637_1_0_1 |
InParanoidi | P07273 |
OMAi | TTFCECT |
Family and domain databases
Gene3Di | 1.10.472.30, 1 hit 1.20.930.10, 1 hit |
InterProi | View protein in InterPro IPR035100, TF_IIS-typ IPR003617, TFIIS/CRSP70_N_sub IPR035441, TFIIS/LEDGF_dom_sf IPR003618, TFIIS_cen_dom IPR036575, TFIIS_cen_dom_sf IPR017923, TFIIS_N IPR006289, TFSII IPR001222, Znf_TFIIS |
Pfami | View protein in Pfam PF08711, Med26, 1 hit PF01096, TFIIS_C, 1 hit PF07500, TFIIS_M, 1 hit |
PIRSFi | PIRSF006704, TF_IIS, 1 hit |
SMARTi | View protein in SMART SM00510, TFS2M, 1 hit SM00509, TFS2N, 1 hit SM00440, ZnF_C2C2, 1 hit |
SUPFAMi | SSF46942, SSF46942, 1 hit SSF47676, SSF47676, 1 hit |
TIGRFAMsi | TIGR01385, TFSII, 1 hit |
PROSITEi | View protein in PROSITE PS51321, TFIIS_CENTRAL, 1 hit PS51319, TFIIS_N, 1 hit PS00466, ZF_TFIIS_1, 1 hit PS51133, ZF_TFIIS_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P07273-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDSKEVLVHV KNLEKNKSND AAVLEILHVL DKEFVPTEKL LRETKVGVEV
60 70 80 90 100
NKFKKSTNVE ISKLVKKMIS SWKDAINKNK RSRQAQQHHQ DHAPGNAEDK
110 120 130 140 150
TTVGESVNGV QQPASSQSDA MKQDKYVSTK PRNSKNDGVD TAIYHHKLRD
160 170 180 190 200
QVLKALYDVL AKESEHPPQS ILHTAKAIES EMNKVNNCDT NEAAYKARYR
210 220 230 240 250
IIYSNVISKN NPDLKHKIAN GDITPEFLAT CDAKDLAPAP LKQKIEEIAK
260 270 280 290 300
QNLYNAQGAT IERSVTDRFT CGKCKEKKVS YYQLQTRSAD EPLTTFCTCE
ACGNRWKFS
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 35 | V → F in AAA88734 (PubMed:1850099).Curated | 1 | |
Sequence conflicti | 35 | V → F in AAA34580 (PubMed:1850099).Curated | 1 | |
Sequence conflicti | 74 – 77 | DAIN → AQLI in AAA88734 (PubMed:1850099).Curated | 4 | |
Sequence conflicti | 74 – 77 | DAIN → AQLI in AAA34580 (PubMed:1850099).Curated | 4 | |
Sequence conflicti | 82 | S → C in AAA88734 (PubMed:1850099).Curated | 1 | |
Sequence conflicti | 82 | S → C in AAA34580 (PubMed:1850099).Curated | 1 | |
Sequence conflicti | 85 | A → P in AAA88734 (PubMed:1850099).Curated | 1 | |
Sequence conflicti | 85 | A → P in AAA34580 (PubMed:1850099).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M36724 Genomic DNA Translation: AAA88734.1 D12478 Genomic DNA Translation: BAA02046.1 M60770 Genomic DNA Translation: AAA34580.1 Z72565 Genomic DNA Translation: CAA96744.1 X00047 Genomic DNA Translation: CAA24928.1 BK006941 Genomic DNA Translation: DAA08057.1 |
PIRi | A42921 |
RefSeqi | NP_011472.1, NM_001180908.1 |
Genome annotation databases
EnsemblFungii | YGL043W_mRNA; YGL043W; YGL043W |
GeneIDi | 852839 |
KEGGi | sce:YGL043W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M36724 Genomic DNA Translation: AAA88734.1 D12478 Genomic DNA Translation: BAA02046.1 M60770 Genomic DNA Translation: AAA34580.1 Z72565 Genomic DNA Translation: CAA96744.1 X00047 Genomic DNA Translation: CAA24928.1 BK006941 Genomic DNA Translation: DAA08057.1 |
PIRi | A42921 |
RefSeqi | NP_011472.1, NM_001180908.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1ENW | NMR | - | A | 131-240 | [»] | |
1EO0 | NMR | - | A | 1-77 | [»] | |
1PQV | X-ray | 3.80 | S | 1-309 | [»] | |
1Y1V | X-ray | 3.80 | S | 131-309 | [»] | |
1Y1Y | X-ray | 4.00 | S | 131-309 | [»] | |
3GTM | X-ray | 3.80 | S | 147-309 | [»] | |
3PO3 | X-ray | 3.30 | S | 132-309 | [»] | |
5FMF | electron microscopy | 6.00 | 2 | 136-309 | [»] | |
BMRBi | P07273 | |||||
SMRi | P07273 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 33205, 587 interactors |
DIPi | DIP-2307N |
IntActi | P07273, 7 interactors |
MINTi | P07273 |
STRINGi | 4932.YGL043W |
PTM databases
iPTMneti | P07273 |
Proteomic databases
MaxQBi | P07273 |
PaxDbi | P07273 |
PRIDEi | P07273 |
Genome annotation databases
EnsemblFungii | YGL043W_mRNA; YGL043W; YGL043W |
GeneIDi | 852839 |
KEGGi | sce:YGL043W |
Organism-specific databases
EuPathDBi | FungiDB:YGL043W |
SGDi | S000003011, DST1 |
Phylogenomic databases
eggNOGi | KOG1105, Eukaryota |
GeneTreei | ENSGT00960000189196 |
HOGENOMi | CLU_037637_1_0_1 |
InParanoidi | P07273 |
OMAi | TTFCECT |
Enzyme and pathway databases
Reactomei | R-SCE-674695, RNA Polymerase II Pre-transcription Events R-SCE-6782135, Dual incision in TC-NER R-SCE-6782210, Gap-filling DNA repair synthesis and ligation in TC-NER R-SCE-6796648, TP53 Regulates Transcription of DNA Repair Genes R-SCE-75955, RNA Polymerase II Transcription Elongation |
Miscellaneous databases
EvolutionaryTracei | P07273 |
PROi | PR:P07273 |
RNActi | P07273, protein |
Family and domain databases
Gene3Di | 1.10.472.30, 1 hit 1.20.930.10, 1 hit |
InterProi | View protein in InterPro IPR035100, TF_IIS-typ IPR003617, TFIIS/CRSP70_N_sub IPR035441, TFIIS/LEDGF_dom_sf IPR003618, TFIIS_cen_dom IPR036575, TFIIS_cen_dom_sf IPR017923, TFIIS_N IPR006289, TFSII IPR001222, Znf_TFIIS |
Pfami | View protein in Pfam PF08711, Med26, 1 hit PF01096, TFIIS_C, 1 hit PF07500, TFIIS_M, 1 hit |
PIRSFi | PIRSF006704, TF_IIS, 1 hit |
SMARTi | View protein in SMART SM00510, TFS2M, 1 hit SM00509, TFS2N, 1 hit SM00440, ZnF_C2C2, 1 hit |
SUPFAMi | SSF46942, SSF46942, 1 hit SSF47676, SSF47676, 1 hit |
TIGRFAMsi | TIGR01385, TFSII, 1 hit |
PROSITEi | View protein in PROSITE PS51321, TFIIS_CENTRAL, 1 hit PS51319, TFIIS_N, 1 hit PS00466, ZF_TFIIS_1, 1 hit PS51133, ZF_TFIIS_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TFS2_YEAST | |
Accessioni | P07273Primary (citable) accession number: P07273 Secondary accession number(s): D6VU96, P24535 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1988 |
Last sequence update: | October 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 209 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome VII
Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names