UniProtKB - P07271 (PIF1_YEAST)
ATP-dependent DNA helicase PIF1
PIF1
Functioni
Miscellaneous
Catalytic activityi
- EC:3.6.4.12UniRule annotation3 Publications
Cofactori
Kineticsi
- KM=1.3 mM for ATP1 Publication
- KM=0.4 mM for dATP1 Publication
pH dependencei
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 258 – 265 | ATPUniRule annotation | 8 | |
DNA bindingi | 727 – 746 | UniRule annotationAdd BLAST | 20 |
GO - Molecular functioni
- 5'-3' DNA helicase activity Source: UniProtKB-UniRule
- ATP binding Source: UniProtKB-UniRule
- DNA helicase activity Source: SGD
- G-quadruplex DNA binding Source: SGD
- single-stranded DNA binding Source: SGD
- telomerase inhibitor activity Source: SGD
GO - Biological processi
- chromosome organization Source: SGD
- DNA duplex unwinding Source: GO_Central
- DNA recombination Source: SGD
- DNA replication Source: GO_Central
- DNA strand renaturation Source: SGD
- DNA unwinding involved in DNA replication Source: SGD
- double-strand break repair via break-induced replication Source: SGD
- G-quadruplex DNA unwinding Source: SGD
- mitochondrial genome maintenance Source: SGD
- ncRNA transcription Source: SGD
- negative regulation of telomere maintenance via telomerase Source: SGD
- replication fork reversal Source: SGD
- telomere maintenance Source: SGD
- telomere maintenance via recombination Source: SGD
Keywordsi
Molecular function | DNA-binding, Helicase, Hydrolase |
Biological process | DNA damage, DNA recombination, DNA repair |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 3.6.4.12, 984 |
Names & Taxonomyi
Protein namesi | Recommended name: ATP-dependent DNA helicase PIF1UniRule annotation (EC:3.6.4.12UniRule annotation)Alternative name(s): DNA repair and recombination helicase PIF1UniRule annotation Petite integration frequency protein 1 Telomere stability protein 1 |
Gene namesi | Ordered Locus Names:YML061C ORF Names:YM9958.01C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YML061C |
SGDi | S000004526, PIF1 |
Subcellular locationi
Nucleus
- nucleolus 2 Publications
Note: Mainly concentrated in the nucleolus, and occasionally redistributes to single nuclear foci outside the nucleolus, probably sites of DNA repair.1 Publication
Mitochondrion
- Mitochondrion inner membrane 4 Publications; Peripheral membrane protein 1 Publication; Matrix side 1 Publication
Note: Bound to the mitochondrial inner membrane either directly or indirectly via a protein complex.1 Publication
Mitochondrion
- mitochondrial inner membrane Source: UniProtKB-SubCell
- mitochondrial membrane Source: SGD
- mitochondrion Source: SGD
Nucleus
- nuclear replication fork Source: SGD
- nucleolus Source: UniProtKB-SubCell
- nucleus Source: SGD
Other locations
- replication fork Source: SGD
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion inner membrane, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 1 | M → A in PIF1-m1; loss of mitochondrial function. 1 Publication | 1 | |
Mutagenesisi | 40 | M → A in PIF1-m2; loss of nuclear function. 1 Publication | 1 | |
Mutagenesisi | 264 | K → A or R: Abolishes helicase activity resulting in elongated telomeres; binds normally to DNA substrates. 3 Publications | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 45 | Mitochondrion1 PublicationAdd BLAST | 45 | |
ChainiPRO_0000013269 | 46 – 859 | ATP-dependent DNA helicase PIF1Add BLAST | 814 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 70 | PhosphoserineCombined sources | 1 | |
Modified residuei | 72 | PhosphoserineCombined sources | 1 | |
Modified residuei | 169 | PhosphoserineCombined sources | 1 | |
Modified residuei | 584 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | P07271 |
PRIDEi | P07271 |
PTM databases
iPTMneti | P07271 |
Expressioni
Inductioni
Interactioni
Subunit structurei
Monomer in solution. DNA binding induces dimerization. Associates with mitochondrial and telomeric DNA. Binding to mtDNA is non-specific and the protein seems to coat the entire mtDNA molecule. Binds to the telomerase RNA TLC1.
Interacts with the mitochondrial single-strand DNA-binding protein RIM1.
UniRule annotation4 PublicationsBinary interactionsi
P07271
With | #Exp. | IntAct |
---|---|---|
SMT3 [Q12306] | 2 | EBI-13404,EBI-17490 |
Protein-protein interaction databases
BioGRIDi | 35105, 192 interactors |
DIPi | DIP-4448N |
IntActi | P07271, 3 interactors |
MINTi | P07271 |
STRINGi | 4932.YML061C |
Miscellaneous databases
RNActi | P07271, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P07271 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG0987, Eukaryota |
GeneTreei | ENSGT00530000063561 |
HOGENOMi | CLU_001613_0_0_1 |
InParanoidi | P07271 |
OMAi | VEPEQWT |
Family and domain databases
HAMAPi | MF_03176, PIF1, 1 hit |
InterProi | View protein in InterPro IPR010285, DNA_helicase_pif1-like IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF05970, PIF1, 2 hits |
SUPFAMi | SSF52540, SSF52540, 2 hits |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative initiation. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MPKWIRSTLN HIIPRRPFIC SFNSFLLLKN VSHAKLSFSM SSRGFRSNNF
60 70 80 90 100
IQAQLKHPSI LSKEDLDLLS DSDDWEEPDC IQLETEKQEK KIITDIHKED
110 120 130 140 150
PVDKKPMRDK NVMNFINKDS PLSWNDMFKP SIIQPPQLIS ENSFDQSSQK
160 170 180 190 200
KSRSTGFKNP LRPALKKESS FDELQNNSIS QERSLEMINE NEKKKMQFGE
210 220 230 240 250
KIAVLTQRPS FTELQNDQDD SNLNPHNGVK VKIPICLSKE QESIIKLAEN
260 270 280 290 300
GHNIFYTGSA GTGKSILLRE MIKVLKGIYG RENVAVTAST GLAACNIGGI
310 320 330 340 350
TIHSFAGIGL GKGDADKLYK KVRRSRKHLR RWENIGALVV DEISMLDAEL
360 370 380 390 400
LDKLDFIARK IRKNHQPFGG IQLIFCGDFF QLPPVSKDPN RPTKFAFESK
410 420 430 440 450
AWKEGVKMTI MLQKVFRQRG DVKFIDMLNR MRLGNIDDET EREFKKLSRP
460 470 480 490 500
LPDDEIIPAE LYSTRMEVER ANNSRLSKLP GQVHIFNAID GGALEDEELK
510 520 530 540 550
ERLLQNFLAP KELHLKVGAQ VMMVKNLDAT LVNGSLGKVI EFMDPETYFC
560 570 580 590 600
YEALTNDPSM PPEKLETWAE NPSKLKAAME REQSDGEESA VASRKSSVKE
610 620 630 640 650
GFAKSDIGEP VSPLDSSVFD FMKRVKTDDE VVLENIKRKE QLMQTIHQNS
660 670 680 690 700
AGKRRLPLVR FKASDMSTRM VLVEPEDWAI EDENEKPLVS RVQLPLMLAW
710 720 730 740 750
SLSIHKSQGQ TLPKVKVDLR RVFEKGQAYV ALSRAVSREG LQVLNFDRTR
760 770 780 790 800
IKAHQKVIDF YLTLSSAESA YKQLEADEQV KKRKLDYAPG PKYKAKSKSK
810 820 830 840 850
SNSPAPISAT TQSNNGIAAM LQRHSRKRFQ LKKESNSNQV HSLVSDEPRG
QDTEDHILE
The sequence of this isoform differs from the canonical sequence as follows:
1-39: Missing.
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 309 | Missing in CAA28953 (PubMed:3038524).Curated | 1 | |
Sequence conflicti | 322 | V → VG in CAA28953 (PubMed:3038524).Curated | 1 | |
Sequence conflicti | 426 | D → E in CAA28953 (PubMed:3038524).Curated | 1 | |
Sequence conflicti | 800 – 801 | Missing in CAA28953 (PubMed:3038524).Curated | 2 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_034601 | 1 – 39 | Missing in isoform Nuclear. CuratedAdd BLAST | 39 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X05342 Genomic DNA Translation: CAA28953.1 Z46729 Genomic DNA Translation: CAA86714.1 Z38114 Genomic DNA Translation: CAA86260.1 BK006946 Genomic DNA Translation: DAA09836.1 |
PIRi | A29457 |
RefSeqi | NP_013650.1, NM_001182420.1 [P07271-1] |
Genome annotation databases
EnsemblFungii | YML061C_mRNA; YML061C; YML061C [P07271-1] |
GeneIDi | 854941 |
KEGGi | sce:YML061C |
Keywords - Coding sequence diversityi
Alternative initiationSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X05342 Genomic DNA Translation: CAA28953.1 Z46729 Genomic DNA Translation: CAA86714.1 Z38114 Genomic DNA Translation: CAA86260.1 BK006946 Genomic DNA Translation: DAA09836.1 |
PIRi | A29457 |
RefSeqi | NP_013650.1, NM_001182420.1 [P07271-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5O6B | X-ray | 2.03 | A/B | 237-780 | [»] | |
5O6D | X-ray | 3.28 | A/B | 237-780 | [»] | |
5O6E | X-ray | 3.35 | A/B | 237-780 | [»] | |
6E49 | X-ray | 2.90 | D/E/F | 815-831 | [»] | |
SMRi | P07271 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 35105, 192 interactors |
DIPi | DIP-4448N |
IntActi | P07271, 3 interactors |
MINTi | P07271 |
STRINGi | 4932.YML061C |
PTM databases
iPTMneti | P07271 |
Proteomic databases
PaxDbi | P07271 |
PRIDEi | P07271 |
Genome annotation databases
EnsemblFungii | YML061C_mRNA; YML061C; YML061C [P07271-1] |
GeneIDi | 854941 |
KEGGi | sce:YML061C |
Organism-specific databases
EuPathDBi | FungiDB:YML061C |
SGDi | S000004526, PIF1 |
Phylogenomic databases
eggNOGi | KOG0987, Eukaryota |
GeneTreei | ENSGT00530000063561 |
HOGENOMi | CLU_001613_0_0_1 |
InParanoidi | P07271 |
OMAi | VEPEQWT |
Enzyme and pathway databases
BRENDAi | 3.6.4.12, 984 |
Miscellaneous databases
PROi | PR:P07271 |
RNActi | P07271, protein |
Family and domain databases
HAMAPi | MF_03176, PIF1, 1 hit |
InterProi | View protein in InterPro IPR010285, DNA_helicase_pif1-like IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF05970, PIF1, 2 hits |
SUPFAMi | SSF52540, SSF52540, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PIF1_YEAST | |
Accessioni | P07271Primary (citable) accession number: P07271 Secondary accession number(s): D6VZB2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1988 |
Last sequence update: | July 1, 2008 | |
Last modified: | December 2, 2020 | |
This is version 189 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Yeast chromosome XIII
Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD