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Protein

Saccharopepsin

Gene

PEP4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Aspartyl protease implicated in the post-translational regulation of S.cerevisiae vacuolar proteinases. Acts on YSCB, on YSCY and on itself.

Catalytic activityi

Hydrolysis of proteins with broad specificity for peptide bonds. Cleaves -Leu-Leu-|-Val-Tyr- bond in a synthetic substrate. Does not act on esters of Tyr or Arg.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei109PROSITE-ProRule annotation1
Active sitei294PROSITE-ProRule annotation1

GO - Molecular functioni

  • aspartic-type endopeptidase activity Source: CAFA
  • disordered domain specific binding Source: CAFA
  • oligosaccharide binding Source: CAFA
  • peptidase activity Source: SGD

GO - Biological processi

  • autophagy Source: SGD
  • cellular response to starvation Source: SGD
  • fungal-type cell wall organization Source: GO_Central
  • lysosomal microautophagy Source: SGD
  • macroautophagy Source: SGD
  • protein localization by the Cvt pathway Source: SGD
  • proteolysis involved in cellular protein catabolic process Source: SGD
  • vacuolar protein processing Source: SGD

Keywordsi

Molecular functionAspartyl protease, Hydrolase, Protease

Enzyme and pathway databases

BioCyciYEAST:YPL154C-MONOMER
BRENDAi3.4.23.25 984
ReactomeiR-SCE-2132295 MHC class II antigen presentation
R-SCE-6798695 Neutrophil degranulation

Protein family/group databases

MEROPSiA01.018

Names & Taxonomyi

Protein namesi
Recommended name:
Saccharopepsin (EC:3.4.23.25)
Alternative name(s):
Aspartate protease
Short name:
PrA
Short name:
Proteinase A
Carboxypeptidase Y-deficient protein 4
Proteinase YSCA
Gene namesi
Name:PEP4
Synonyms:PHO9, PRA1
Ordered Locus Names:YPL154C
ORF Names:P2585
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL154C
SGDiS000006075 PEP4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Vacuole

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi294D → A: Inactivation. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL4451

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 221 PublicationAdd BLAST22
PropeptideiPRO_000002587323 – 76Activation peptide1 PublicationAdd BLAST54
ChainiPRO_000002587477 – 405SaccharopepsinAdd BLAST329

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi122 ↔ 127
Glycosylationi144N-linked (GlcNAc...) asparagine1
Disulfide bondi328 ↔ 361
Glycosylationi345N-linked (GlcNAc...) asparagine1

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

MaxQBiP07267
PaxDbiP07267
PRIDEiP07267

2D gel databases

UCD-2DPAGEiP07267

Miscellaneous databases

PMAP-CutDBiP07267

Interactioni

Binary interactionsi

Show more details

GO - Molecular functioni

  • disordered domain specific binding Source: CAFA

Protein-protein interaction databases

BioGridi36029, 179 interactors
DIPiDIP-4442N
IntActiP07267, 9 interactors
MINTiP07267
STRINGi4932.YPL154C

Chemistry databases

BindingDBiP07267

Structurei

Secondary structure

1405
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi79 – 82Combined sources4
Beta strandi84 – 86Combined sources3
Turni87 – 89Combined sources3
Beta strandi90 – 97Combined sources8
Turni98 – 101Combined sources4
Beta strandi102 – 109Combined sources8
Beta strandi115 – 119Combined sources5
Helixi125 – 128Combined sources4
Helixi135 – 137Combined sources3
Beta strandi142 – 152Combined sources11
Beta strandi155 – 168Combined sources14
Beta strandi171 – 183Combined sources13
Helixi186 – 189Combined sources4
Beta strandi195 – 199Combined sources5
Helixi203 – 205Combined sources3
Helixi207 – 209Combined sources3
Helixi213 – 219Combined sources7
Beta strandi224 – 232Combined sources9
Helixi235 – 237Combined sources3
Beta strandi240 – 249Combined sources10
Helixi252 – 254Combined sources3
Beta strandi255 – 263Combined sources9
Turni267 – 270Combined sources4
Beta strandi271 – 279Combined sources9
Beta strandi282 – 285Combined sources4
Beta strandi290 – 293Combined sources4
Beta strandi300 – 302Combined sources3
Helixi304 – 314Combined sources11
Beta strandi317 – 319Combined sources3
Beta strandi322 – 326Combined sources5
Helixi328 – 333Combined sources6
Beta strandi337 – 341Combined sources5
Beta strandi344 – 348Combined sources5
Turni350 – 352Combined sources3
Beta strandi353 – 357Combined sources5
Beta strandi360 – 363Combined sources4
Beta strandi365 – 367Combined sources3
Turni372 – 374Combined sources3
Beta strandi376 – 380Combined sources5
Helixi382 – 385Combined sources4
Beta strandi388 – 393Combined sources6
Turni394 – 397Combined sources4
Beta strandi398 – 404Combined sources7

3D structure databases

ProteinModelPortaliP07267
SMRiP07267
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07267

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini91 – 402Peptidase A1PROSITE-ProRule annotationAdd BLAST312

Sequence similaritiesi

Belongs to the peptidase A1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

GeneTreeiENSGT00760000118929
HOGENOMiHOG000197681
InParanoidiP07267
KOiK01381
OMAiWNGQYTV
OrthoDBiEOG092C33N0

Family and domain databases

CDDicd05488 Proteinase_A_fungi, 1 hit
Gene3Di2.40.70.10, 2 hits
InterProiView protein in InterPro
IPR001461 Aspartic_peptidase_A1
IPR001969 Aspartic_peptidase_AS
IPR033121 PEPTIDASE_A1
IPR021109 Peptidase_aspartic_dom_sf
IPR033819 Saccharopepsin
PANTHERiPTHR13683 PTHR13683, 1 hit
PfamiView protein in Pfam
PF00026 Asp, 1 hit
PRINTSiPR00792 PEPSIN
SUPFAMiSSF50630 SSF50630, 2 hits
PROSITEiView protein in PROSITE
PS00141 ASP_PROTEASE, 2 hits
PS51767 PEPTIDASE_A1, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07267-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFSLKALLPL ALLLVSANQV AAKVHKAKIY KHELSDEMKE VTFEQHLAHL
60 70 80 90 100
GQKYLTQFEK ANPEVVFSRE HPFFTEGGHD VPLTNYLNAQ YYTDITLGTP
110 120 130 140 150
PQNFKVILDT GSSNLWVPSN ECGSLACFLH SKYDHEASSS YKANGTEFAI
160 170 180 190 200
QYGTGSLEGY ISQDTLSIGD LTIPKQDFAE ATSEPGLTFA FGKFDGILGL
210 220 230 240 250
GYDTISVDKV VPPFYNAIQQ DLLDEKRFAF YLGDTSKDTE NGGEATFGGI
260 270 280 290 300
DESKFKGDIT WLPVRRKAYW EVKFEGIGLG DEYAELESHG AAIDTGTSLI
310 320 330 340 350
TLPSGLAEMI NAEIGAKKGW TGQYTLDCNT RDNLPDLIFN FNGYNFTIGP
360 370 380 390 400
YDYTLEVSGS CISAITPMDF PEPVGPLAIV GDAFLRKYYS IYDLGNNAVG

LAKAI
Length:405
Mass (Da):44,499
Last modified:April 1, 1988 - v1
Checksum:iB0AA36BA098D2BF7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13358 Genomic DNA Translation: AAB63975.1
X96770 Genomic DNA Translation: CAA65567.1
Z73510 Genomic DNA Translation: CAA97859.1
Z11963 Genomic DNA Translation: CAA78020.1
BK006949 Genomic DNA Translation: DAA11280.1
PIRiA25379
RefSeqiNP_015171.1, NM_001183968.1

Genome annotation databases

EnsemblFungiiYPL154C; YPL154C; YPL154C
GeneIDi855949
KEGGisce:YPL154C

Similar proteinsi

Entry informationi

Entry nameiCARP_YEAST
AccessioniPrimary (citable) accession number: P07267
Secondary accession number(s): D6W3L4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: July 18, 2018
This is version 197 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

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