UniProtKB - P07258 (CARA_YEAST)
Protein
Carbamoyl-phosphate synthase arginine-specific small chain
Gene
CPA1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Miscellaneous
In eukaryotes this enzyme is synthesized by two pathway-specific (arginine and pyrimidine) under separate control.
Present with 2580 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- 2 ATP + H2O + hydrogencarbonate + L-glutamine = 2 ADP + carbamoyl phosphate + 2 H+ + L-glutamate + phosphateEC:6.3.5.5
: L-arginine biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes carbamoyl phosphate from bicarbonate.Proteins known to be involved in this subpathway in this organism are:
- Carbamoyl-phosphate synthase arginine-specific large chain (CPA2), Carbamoyl-phosphate synthase arginine-specific small chain (CPA1)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes carbamoyl phosphate from bicarbonate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 264 | NucleophileBy similarity | 1 | |
Active sitei | 349 | By similarity | 1 | |
Active sitei | 351 | By similarity | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Source: UniProtKB-EC
GO - Biological processi
- 'de novo' pyrimidine nucleobase biosynthetic process Source: InterPro
- arginine biosynthetic process Source: SGD
- glutamine metabolic process Source: InterPro
- nitrogen compound metabolic process Source: GO_Central
Keywordsi
Molecular function | Ligase |
Biological process | Amino-acid biosynthesis, Arginine biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | MetaCyc:YOR303W-MONOMER |
UniPathwayi | UPA00068;UER00171 |
Names & Taxonomyi
Protein namesi | Recommended name: Carbamoyl-phosphate synthase arginine-specific small chain (EC:6.3.5.5)Short name: CPS-A Alternative name(s): Arginine-specific carbamoyl-phosphate synthetase, glutamine chain |
Gene namesi | Name:CPA1 Ordered Locus Names:YOR303W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000005829, CPA1 |
VEuPathDBi | FungiDB:YOR303W |
Subcellular locationi
Other locations
Other locations
- carbamoyl-phosphate synthase complex Source: SGD
- cytoplasm Source: SGD
- cytoplasmic stress granule Source: SGD
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000112372 | 1 – 411 | Carbamoyl-phosphate synthase arginine-specific small chainAdd BLAST | 411 |
Proteomic databases
MaxQBi | P07258 |
PaxDbi | P07258 |
PRIDEi | P07258 |
Interactioni
Subunit structurei
Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.
Protein-protein interaction databases
BioGRIDi | 34691, 31 interactors |
ComplexPortali | CPX-579, Carbamoyl-phosphate synthase arginine-specific |
DIPi | DIP-1024N |
IntActi | P07258, 5 interactors |
MINTi | P07258 |
STRINGi | 4932.YOR303W |
Miscellaneous databases
RNActi | P07258, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 185 – 376 | Glutamine amidotransferase type-1Add BLAST | 192 |
Sequence similaritiesi
Belongs to the CarA family.Curated
Phylogenomic databases
eggNOGi | KOG0370, Eukaryota |
GeneTreei | ENSGT00940000157241 |
HOGENOMi | CLU_035901_1_1_1 |
InParanoidi | P07258 |
OMAi | CFNTGMT |
Family and domain databases
CDDi | cd01744, GATase1_CPSase, 1 hit |
Gene3Di | 3.40.50.880, 1 hit 3.50.30.20, 1 hit |
HAMAPi | MF_01209, CPSase_S_chain, 1 hit |
InterProi | View protein in InterPro IPR006274, CarbamoylP_synth_ssu IPR002474, CarbamoylP_synth_ssu_N IPR036480, CarbP_synth_ssu_N_sf IPR029062, Class_I_gatase-like IPR035686, CPSase_GATase1 IPR017926, GATASE |
Pfami | View protein in Pfam PF00988, CPSase_sm_chain, 1 hit PF00117, GATase, 1 hit |
SMARTi | View protein in SMART SM01097, CPSase_sm_chain, 1 hit |
SUPFAMi | SSF52021, SSF52021, 1 hit SSF52317, SSF52317, 1 hit |
TIGRFAMsi | TIGR01368, CPSaseIIsmall, 1 hit |
PROSITEi | View protein in PROSITE PS51273, GATASE_TYPE_1, 1 hit |
i Sequence
Sequence statusi: Complete.
P07258-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSSAATKATF CIQNGPSFEG ISFGANKSVA GETVFTTSLV GYPESMTDPS
60 70 80 90 100
YRGQILVFTQ PLIGNYGVPS GEARDEYNLL KYFESPHIHV VGIVVAEYAY
110 120 130 140 150
QYSHWTAVES LAQWCQREGV AAITGVDTRE LVQYLREQGS SLGRITLADH
160 170 180 190 200
DPVPYVNPMK TNLVAQVTTK KPFHVSALPG KAKANVALID CGVKENIIRC
210 220 230 240 250
LVKRGANVTV FPYDYRIQDV ASEFDGIFLS NGPGNPELCQ ATISNVRELL
260 270 280 290 300
NNPVYDCIPI FGICLGHQLL ALASGASTHK LKYGNRAHNI PAMDLTTGQC
310 320 330 340 350
HITSQNHGYA VDPETLPKDQ WKPYFVNLND KSNEGMIHLQ RPIFSTQFHP
360 370 380 390 400
EAKGGPLDTA ILFDKFFDNI EKYQLQSQAK SSISLKVTYS TDKSRLQSIN
410
VTKLAKERVL F
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X01764 Genomic DNA Translation: CAA25905.1 K02132 Genomic DNA Translation: AAA34525.1 Z75211 Genomic DNA Translation: CAA99621.1 M16690 Genomic DNA Translation: AAA34527.1 BK006948 Genomic DNA Translation: DAA11068.1 |
PIRi | S67207, SYBYCS |
RefSeqi | NP_014947.3, NM_001183722.3 |
Genome annotation databases
EnsemblFungii | YOR303W_mRNA; YOR303W; YOR303W |
GeneIDi | 854479 |
KEGGi | sce:YOR303W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X01764 Genomic DNA Translation: CAA25905.1 K02132 Genomic DNA Translation: AAA34525.1 Z75211 Genomic DNA Translation: CAA99621.1 M16690 Genomic DNA Translation: AAA34527.1 BK006948 Genomic DNA Translation: DAA11068.1 |
PIRi | S67207, SYBYCS |
RefSeqi | NP_014947.3, NM_001183722.3 |
3D structure databases
SMRi | P07258 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 34691, 31 interactors |
ComplexPortali | CPX-579, Carbamoyl-phosphate synthase arginine-specific |
DIPi | DIP-1024N |
IntActi | P07258, 5 interactors |
MINTi | P07258 |
STRINGi | 4932.YOR303W |
Proteomic databases
MaxQBi | P07258 |
PaxDbi | P07258 |
PRIDEi | P07258 |
Genome annotation databases
EnsemblFungii | YOR303W_mRNA; YOR303W; YOR303W |
GeneIDi | 854479 |
KEGGi | sce:YOR303W |
Organism-specific databases
SGDi | S000005829, CPA1 |
VEuPathDBi | FungiDB:YOR303W |
Phylogenomic databases
eggNOGi | KOG0370, Eukaryota |
GeneTreei | ENSGT00940000157241 |
HOGENOMi | CLU_035901_1_1_1 |
InParanoidi | P07258 |
OMAi | CFNTGMT |
Enzyme and pathway databases
UniPathwayi | UPA00068;UER00171 |
BioCyci | MetaCyc:YOR303W-MONOMER |
Miscellaneous databases
PROi | PR:P07258 |
RNActi | P07258, protein |
Family and domain databases
CDDi | cd01744, GATase1_CPSase, 1 hit |
Gene3Di | 3.40.50.880, 1 hit 3.50.30.20, 1 hit |
HAMAPi | MF_01209, CPSase_S_chain, 1 hit |
InterProi | View protein in InterPro IPR006274, CarbamoylP_synth_ssu IPR002474, CarbamoylP_synth_ssu_N IPR036480, CarbP_synth_ssu_N_sf IPR029062, Class_I_gatase-like IPR035686, CPSase_GATase1 IPR017926, GATASE |
Pfami | View protein in Pfam PF00988, CPSase_sm_chain, 1 hit PF00117, GATase, 1 hit |
SMARTi | View protein in SMART SM01097, CPSase_sm_chain, 1 hit |
SUPFAMi | SSF52021, SSF52021, 1 hit SSF52317, SSF52317, 1 hit |
TIGRFAMsi | TIGR01368, CPSaseIIsmall, 1 hit |
PROSITEi | View protein in PROSITE PS51273, GATASE_TYPE_1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CARA_YEAST | |
Accessioni | P07258Primary (citable) accession number: P07258 Secondary accession number(s): D6W302 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1988 |
Last sequence update: | April 1, 1988 | |
Last modified: | February 10, 2021 | |
This is version 188 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XV
Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families