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Protein

SPARC

Gene

Sparc

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca2+ with a low affinity and an EF-hand loop that binds a Ca2+ ion with a high affinity.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi273 – 284By similarityAdd BLAST12

GO - Molecular functioni

  • calcium ion binding Source: CAFA
  • collagen binding Source: MGI
  • extracellular matrix binding Source: MGI

GO - Biological processi

Keywordsi

LigandCalcium, Copper, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-114608 Platelet degranulation
R-MMU-3000178 ECM proteoglycans
R-MMU-3000497 Scavenging by Class H Receptors

Names & Taxonomyi

Protein namesi
Recommended name:
SPARC
Alternative name(s):
Basement-membrane protein 40
Short name:
BM-40
Osteonectin
Short name:
ON
Secreted protein acidic and rich in cysteine
Gene namesi
Name:Sparc
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:98373 Sparc

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Basement membrane, Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 171 PublicationAdd BLAST17
ChainiPRO_000002030518 – 302SPARCAdd BLAST285

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi71 ↔ 82PROSITE-ProRule annotation
Disulfide bondi76 ↔ 92PROSITE-ProRule annotation
Disulfide bondi94 ↔ 129PROSITE-ProRule annotation
Disulfide bondi100 ↔ 122PROSITE-ProRule annotation
Disulfide bondi111 ↔ 148PROSITE-ProRule annotation
Glycosylationi115N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi154 ↔ 264
Disulfide bondi272 ↔ 288

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP07214
PeptideAtlasiP07214
PRIDEiP07214

PTM databases

iPTMnetiP07214
PhosphoSitePlusiP07214

Expressioni

Developmental stagei

Expressed at high levels in tissues undergoing morphogenesis, remodeling and wound repair.

Gene expression databases

BgeeiENSMUSG00000018593
CleanExiMM_SPARC
ExpressionAtlasiP07214 baseline and differential
GenevisibleiP07214 MM

Interactioni

Protein-protein interaction databases

BioGridi203423, 2 interactors
IntActiP07214, 4 interactors
MINTiP07214
STRINGi10090.ENSMUSP00000018737

Structurei

3D structure databases

DisProtiDP00052
ProteinModelPortaliP07214
SMRiP07214
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini70 – 92Follistatin-likeAdd BLAST23
Domaini88 – 150Kazal-likePROSITE-ProRule annotationAdd BLAST63
Domaini260 – 295EF-handAdd BLAST36

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi23 – 68Asp/Glu-rich (acidic; binds calcium)Add BLAST46

Sequence similaritiesi

Belongs to the SPARC family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4004 Eukaryota
ENOG41101WW LUCA
GeneTreeiENSGT00510000046787
HOGENOMiHOG000088654
HOVERGENiHBG002746
InParanoidiP07214
OMAiKDFPRRM
OrthoDBiEOG091G0CPG
PhylomeDBiP07214
TreeFamiTF319356

Family and domain databases

InterProiView protein in InterPro
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR003645 Fol_N
IPR015369 Follistatin/Osteonectin_EGF
IPR002350 Kazal_dom
IPR036058 Kazal_dom_sf
IPR001999 Osteonectin_CS
IPR037641 SPARC
IPR019577 SPARC/Testican_Ca-bd-dom
PANTHERiPTHR13866 PTHR13866, 1 hit
PfamiView protein in Pfam
PF09289 FOLN, 1 hit
PF00050 Kazal_1, 1 hit
PF10591 SPARC_Ca_bdg, 1 hit
SMARTiView protein in SMART
SM00274 FOLN, 1 hit
SM00280 KAZAL, 1 hit
SUPFAMiSSF100895 SSF100895, 1 hit
SSF47473 SSF47473, 1 hit
PROSITEiView protein in PROSITE
PS00018 EF_HAND_1, 1 hit
PS51465 KAZAL_2, 1 hit
PS00612 OSTEONECTIN_1, 1 hit
PS00613 OSTEONECTIN_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07214-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRAWIFFLLC LAGRALAAPQ QTEVAEEIVE EETVVEETGV PVGANPVQVE
60 70 80 90 100
MGEFEDGAEE TVEEVVADNP CQNHHCKHGK VCELDESNTP MCVCQDPTSC
110 120 130 140 150
PAPIGEFEKV CSNDNKTFDS SCHFFATKCT LEGTKKGHKL HLDYIGPCKY
160 170 180 190 200
IAPCLDSELT EFPLRMRDWL KNVLVTLYER DEGNNLLTEK QKLRVKKIHE
210 220 230 240 250
NEKRLEAGDH PVELLARDFE KNYNMYIFPV HWQFGQLDQH PIDGYLSHTE
260 270 280 290 300
LAPLRAPLIP MEHCTTRFFE TCDLDNDKYI ALEEWAGCFG IKEQDINKDL

VI
Length:302
Mass (Da):34,450
Last modified:April 1, 1988 - v1
Checksum:iA1FF717CA32F0F5C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04017 mRNA Translation: CAA27642.1
M20692
, M20684, M20685, M20686, M20687, M20688, M20689, M20690, M20691 Genomic DNA Translation: AAA40125.1
BC004638 mRNA Translation: AAH04638.1
CCDSiCCDS24712.1
PIRiA29227 GEMSN
RefSeqiNP_033268.1, NM_009242.5
UniGeneiMm.291442

Genome annotation databases

EnsembliENSMUST00000018737; ENSMUSP00000018737; ENSMUSG00000018593
ENSMUST00000214685; ENSMUSP00000151000; ENSMUSG00000018593
GeneIDi20692
KEGGimmu:20692
UCSCiuc007izh.3 mouse

Similar proteinsi

Entry informationi

Entry nameiSPRC_MOUSE
AccessioniPrimary (citable) accession number: P07214
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: July 18, 2018
This is version 166 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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