UniProtKB - P07214 (SPRC_MOUSE)

BLAST

>sp|P07214|SPRC_MOUSE SPARC OS=Mus musculus OX=10090 GN=Sparc PE=1 SV=1
MRAWIFFLLCLAGRALAAPQQTEVAEEIVEEETVVEETGVPVGANPVQVEMGEFEDGAEE
TVEEVVADNPCQNHHCKHGKVCELDESNTPMCVCQDPTSCPAPIGEFEKVCSNDNKTFDS
SCHFFATKCTLEGTKKGHKLHLDYIGPCKYIAPCLDSELTEFPLRMRDWLKNVLVTLYER
DEGNNLLTEKQKLRVKKIHENEKRLEAGDHPVELLARDFEKNYNMYIFPVHWQFGQLDQH
PIDGYLSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALEEWAGCFGIKEQDINKDL
VI

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History
Entry version 169 (07 Nov 2018)
Sequence version 1 (01 Apr 1988)
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Protein

SPARC

Gene

Sparc

Organism

Mus musculus (Mouse)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca²⁺ with a low affinity and an EF-hand loop that binds a Ca²⁺ ion with a high affinity.

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Calcium bindingⁱ	273 – 284	By similarityAdd BLAST		12

GO - Molecular functionⁱ

calcium ion binding
Source: CAFA
Inferred from direct assayⁱ
- 3427055
collagen binding Source: MGI
extracellular matrix binding
Source: MGI
Inferred from direct assayⁱ
- 18757743
extracellular matrix structural constituent
Source: BHF-UCL
Inferred from high throughput direct assayⁱ
- 28071719

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

bone development
Source: MGI
Inferred from mutant phenotypeⁱ
- 22876197
cellular response to growth factor stimulus
Source: MGI
Inferred from direct assayⁱ
- 7848824
heart development Source: Ensembl
inner ear development Source: Ensembl
lung development Source: MGI
negative regulation of angiogenesis Source: MGI
negative regulation of endothelial cell proliferation Source: MGI
ossification Source: Ensembl
pigmentation
Source: MGI
Inferred from mutant phenotypeⁱ
- 25340873
positive regulation of endothelial cell migration Source: MGI
regulation of cell morphogenesis Source: MGI
regulation of cell proliferation
Source: MGI
Inferred from genetic interactionⁱ
- 17299058
regulation of synapse organization Source: MGI
response to cadmium ion Source: Ensembl
response to calcium ion Source: Ensembl
response to cAMP Source: Ensembl
response to cytokine Source: Ensembl
response to ethanol Source: Ensembl
response to glucocorticoid Source: Ensembl
response to gravity Source: Ensembl
response to L-ascorbic acid Source: Ensembl
response to lead ion Source: Ensembl
response to lipopolysaccharide Source: Ensembl
response to peptide hormone Source: Ensembl
wound healing Source: Ensembl

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Ligand	Calcium, Copper, Metal-binding

Ligand

Calcium, Copper, Metal-binding

Enzyme and pathway databases

Reactomeⁱ	R-MMU-114608 Platelet degranulation R-MMU-3000178 ECM proteoglycans R-MMU-3000497 Scavenging by Class H Receptors

Reactomeⁱ

R-MMU-114608 Platelet degranulation
R-MMU-3000178 ECM proteoglycans
R-MMU-3000497 Scavenging by Class H Receptors

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: SPARC Alternative name(s): Basement-membrane protein 40 Short name: BM-40 Osteonectin Short name: ON Secreted protein acidic and rich in cysteine
Gene namesⁱ	Name:Sparc
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 11

Organism-specific databases

MGIⁱ	MGI:98373 Sparc

Keywords - Cellular componentⁱ

Basement membrane, Extracellular matrix, Secreted

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Signal peptideⁱ	1 – 17	1 PublicationAdd BLAST		17
ChainⁱPRO_0000020305	18 – 302	SPARCAdd BLAST		285

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Disulfide bondⁱ	71 ↔ 82	PROSITE-ProRule annotation
Disulfide bondⁱ	76 ↔ 92	PROSITE-ProRule annotation
Disulfide bondⁱ	94 ↔ 129	PROSITE-ProRule annotation
Disulfide bondⁱ	100 ↔ 122	PROSITE-ProRule annotation
Disulfide bondⁱ	111 ↔ 148	PROSITE-ProRule annotation
Glycosylationⁱ	115	N-linked (GlcNAc...) asparagine1 Publication	1
Disulfide bondⁱ	154 ↔ 264
Disulfide bondⁱ	272 ↔ 288

Post-translational modificationⁱ

N-glycosylated.1 Publication

Keywords - PTMⁱ

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbⁱ	P07214
PeptideAtlas More...PeptideAtlasⁱ	P07214
PRIDEⁱ	P07214

PTM databases

iPTMnetⁱ	P07214
PhosphoSitePlusⁱ	P07214

Expressionⁱ

Developmental stageⁱ

Expressed at high levels in tissues undergoing morphogenesis, remodeling and wound repair.

Gene expression databases

Bgeeⁱ	ENSMUSG00000018593 Expressed in 324 organ(s), highest expression level in vault of skull
CleanExⁱ	MM_SPARC
ExpressionAtlasⁱ	P07214 baseline and differential
Genevisibleⁱ	P07214 MM

Interactionⁱ

Protein-protein interaction databases

BioGridⁱ	203423, 2 interactors
IntActⁱ	P07214, 4 interactors
Molecular INTeraction database More...MINTⁱ	P07214
STRINGⁱ	10090.ENSMUSP00000018737

Structureⁱ

3D structure databases

Database of protein disorder More...DisProtⁱ	DP00052
ProteinModelPortalⁱ	P07214
SMRⁱ	P07214
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Length
Domainⁱ	70 – 92	Follistatin-likeAdd BLAST	23
Domainⁱ	88 – 150	Kazal-likePROSITE-ProRule annotationAdd BLAST	63
Domainⁱ	260 – 295	EF-handAdd BLAST	36

Compositional bias

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Compositional biasⁱ	23 – 68	Asp/Glu-rich (acidic; binds calcium)Add BLAST		46

Sequence similaritiesⁱ

Belongs to the SPARC family.Curated

Keywords - Domainⁱ

Signal

Phylogenomic databases

eggNOGⁱ	KOG4004 Eukaryota ENOG41101WW LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00510000046787
HOGENOMⁱ	HOG000088654
HOVERGENⁱ	HBG002746
InParanoidⁱ	P07214
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0CPG
PhylomeDBⁱ	P07214
TreeFamⁱ	TF319356

Family and domain databases

InterProⁱ	View protein in InterPro IPR011992 EF-hand-dom_pair IPR018247 EF_Hand_1_Ca_BS IPR003645 Fol_N IPR015369 Follistatin/Osteonectin_EGF IPR002350 Kazal_dom IPR036058 Kazal_dom_sf IPR001999 Osteonectin_CS IPR037641 SPARC IPR019577 SPARC/Testican_Ca-bd-dom
PANTHERⁱ	PTHR13866 PTHR13866, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF09289 FOLN, 1 hit PF00050 Kazal_1, 1 hit PF10591 SPARC_Ca_bdg, 1 hit
SMARTⁱ	View protein in SMART SM00274 FOLN, 1 hit SM00280 KAZAL, 1 hit
SUPFAMⁱ	SSF100895 SSF100895, 1 hit SSF47473 SSF47473, 1 hit
PROSITEⁱ	View protein in PROSITE PS00018 EF_HAND_1, 1 hit PS51465 KAZAL_2, 1 hit PS00612 OSTEONECTIN_1, 1 hit PS00613 OSTEONECTIN_2, 1 hit

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show all Align All

P07214-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MRAWIFFLLC LAGRALAAPQ QTEVAEEIVE EETVVEETGV PVGANPVQVE 
        60         70         80         90        100
MGEFEDGAEE TVEEVVADNP CQNHHCKHGK VCELDESNTP MCVCQDPTSC 
       110        120        130        140        150
PAPIGEFEKV CSNDNKTFDS SCHFFATKCT LEGTKKGHKL HLDYIGPCKY 
       160        170        180        190        200
IAPCLDSELT EFPLRMRDWL KNVLVTLYER DEGNNLLTEK QKLRVKKIHE 
       210        220        230        240        250
NEKRLEAGDH PVELLARDFE KNYNMYIFPV HWQFGQLDQH PIDGYLSHTE 
       260        270        280        290        300
LAPLRAPLIP MEHCTTRFFE TCDLDNDKYI ALEEWAGCFG IKEQDINKDL 

VI

Length:302

Mass (Da):34,450

Last modified:April 1, 1988 - v1

Checksum:ⁱA1FF717CA32F0F5C

Computationally mapped potential isoform sequencesⁱ

There are 3 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

Q5NCU4	Q5NCU4_MOUSE	SPARC SPARC	Sparc	301	Annotation score:
Q5NCU3	Q5NCU3_MOUSE	SPARC SPARC	Sparc	172	Annotation score:
A0A1L1SSH9	A0A1L1SSH9_MOUSE	SPARC SPARC	Sparc	332	Annotation score:

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X04017 mRNA Translation: CAA27642.1 M20692 M20691 Genomic DNA Translation: AAA40125.1 BC004638 mRNA Translation: AAH04638.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS24712.1
PIRⁱ	A29227 GEMSN
NCBI Reference Sequences More...RefSeqⁱ	NP_033268.1, NM_009242.5
UniGeneⁱ	Mm.291442

Genome annotation databases

Ensemblⁱ	ENSMUST00000018737; ENSMUSP00000018737; ENSMUSG00000018593 ENSMUST00000214685; ENSMUSP00000151000; ENSMUSG00000018593
GeneIDⁱ	20692
KEGGⁱ	mmu:20692
UCSC genome browser More...UCSCⁱ	uc007izh.3 mouse

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P07214	SPARC		MOUSE		332	UniRef100_P07214
Sparc protein		MOUSE		302

Protein	Similar proteins		Species	Score	Length	Source
P07214	SPARC		MOUSE		332	UniRef90_P07214
Sparc protein		MOUSE		302

Protein	Similar proteins		Species	Score	Length	Source
P07214	SPARC		RAT		301	UniRef50_P07214
SPARC		PIG		300
SPARC (Fragment)		RABIT		273
SPARC		COTJA		298
SPARC		XENLA		300
+412

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X04017 mRNA Translation: CAA27642.1 M20692 M20691 Genomic DNA Translation: AAA40125.1 BC004638 mRNA Translation: AAH04638.1
CCDSⁱ	CCDS24712.1
PIRⁱ	A29227 GEMSN
RefSeqⁱ	NP_033268.1, NM_009242.5
UniGeneⁱ	Mm.291442

3D structure databases

DisProtⁱ	DP00052
ProteinModelPortalⁱ	P07214
SMRⁱ	P07214
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	203423, 2 interactors
IntActⁱ	P07214, 4 interactors
MINTⁱ	P07214
STRINGⁱ	10090.ENSMUSP00000018737

PTM databases

iPTMnetⁱ	P07214
PhosphoSitePlusⁱ	P07214

Proteomic databases

PaxDbⁱ	P07214
PeptideAtlasⁱ	P07214
PRIDEⁱ	P07214

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000018737; ENSMUSP00000018737; ENSMUSG00000018593 ENSMUST00000214685; ENSMUSP00000151000; ENSMUSG00000018593
GeneIDⁱ	20692
KEGGⁱ	mmu:20692
UCSCⁱ	uc007izh.3 mouse

Organism-specific databases

CTDⁱ	6678
MGIⁱ	MGI:98373 Sparc

Phylogenomic databases

eggNOGⁱ	KOG4004 Eukaryota ENOG41101WW LUCA
GeneTreeⁱ	ENSGT00510000046787
HOGENOMⁱ	HOG000088654
HOVERGENⁱ	HBG002746
InParanoidⁱ	P07214
OrthoDBⁱ	EOG091G0CPG
PhylomeDBⁱ	P07214
TreeFamⁱ	TF319356

Enzyme and pathway databases

Reactomeⁱ	R-MMU-114608 Platelet degranulation R-MMU-3000178 ECM proteoglycans R-MMU-3000497 Scavenging by Class H Receptors

Reactomeⁱ

R-MMU-114608 Platelet degranulation
R-MMU-3000178 ECM proteoglycans
R-MMU-3000497 Scavenging by Class H Receptors

Miscellaneous databases

ChiTaRSⁱ	Sparc mouse
Protein Ontology More...PROⁱ	PR:P07214
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000018593 Expressed in 324 organ(s), highest expression level in vault of skull
CleanExⁱ	MM_SPARC
ExpressionAtlasⁱ	P07214 baseline and differential
Genevisibleⁱ	P07214 MM

Family and domain databases

InterProⁱ	View protein in InterPro IPR011992 EF-hand-dom_pair IPR018247 EF_Hand_1_Ca_BS IPR003645 Fol_N IPR015369 Follistatin/Osteonectin_EGF IPR002350 Kazal_dom IPR036058 Kazal_dom_sf IPR001999 Osteonectin_CS IPR037641 SPARC IPR019577 SPARC/Testican_Ca-bd-dom
PANTHERⁱ	PTHR13866 PTHR13866, 1 hit
Pfamⁱ	View protein in Pfam PF09289 FOLN, 1 hit PF00050 Kazal_1, 1 hit PF10591 SPARC_Ca_bdg, 1 hit
SMARTⁱ	View protein in SMART SM00274 FOLN, 1 hit SM00280 KAZAL, 1 hit
SUPFAMⁱ	SSF100895 SSF100895, 1 hit SSF47473 SSF47473, 1 hit
PROSITEⁱ	View protein in PROSITE PS00018 EF_HAND_1, 1 hit PS51465 KAZAL_2, 1 hit PS00612 OSTEONECTIN_1, 1 hit PS00613 OSTEONECTIN_2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	SPRC_MOUSE
Accessionⁱ	P07214Primary (citable) accession number: P07214
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
	Last sequence update:	April 1, 1988
	Last modified:	November 7, 2018
	This is version 169 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Direct protein sequencing, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P07214 (SPRC_MOUSE)

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SPARC

Sparc

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted

Extracellular region or secreted

Nucleus

Other locations

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Compositional bias

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

UniProt is an ELIXIR core data resource

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ