UniProtKB - P07214 (SPRC_MOUSE)
Protein
SPARC
Gene
Sparc
Organism
Mus musculus (Mouse)
Status
Functioni
Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca2+ with a low affinity and an EF-hand loop that binds a Ca2+ ion with a high affinity.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Calcium bindingi | 273 – 284 | By similarityAdd BLAST | 12 |
GO - Molecular functioni
- calcium ion binding Source: CAFA
- collagen binding Source: MGI
- extracellular matrix binding Source: MGI
GO - Biological processi
- anatomical structure development Source: GO_Central
- bone development Source: MGI
- cellular response to growth factor stimulus Source: MGI
- heart development Source: Ensembl
- inner ear development Source: Ensembl
- lung development Source: MGI
- negative regulation of angiogenesis Source: MGI
- negative regulation of endothelial cell proliferation Source: MGI
- ossification Source: Ensembl
- pigmentation Source: MGI
- positive regulation of endothelial cell migration Source: MGI
- regulation of cell morphogenesis Source: MGI
- regulation of cell population proliferation Source: MGI
- regulation of synapse organization Source: MGI
- response to cadmium ion Source: Ensembl
- response to calcium ion Source: Ensembl
- response to cAMP Source: Ensembl
- response to cytokine Source: Ensembl
- response to ethanol Source: Ensembl
- response to glucocorticoid Source: Ensembl
- response to gravity Source: Ensembl
- response to L-ascorbic acid Source: Ensembl
- response to lead ion Source: Ensembl
- response to lipopolysaccharide Source: Ensembl
- response to peptide hormone Source: Ensembl
- wound healing Source: Ensembl
Keywordsi
Ligand | Calcium, Copper, Metal-binding |
Enzyme and pathway databases
Reactomei | R-MMU-114608, Platelet degranulation R-MMU-3000178, ECM proteoglycans R-MMU-3000497, Scavenging by Class H Receptors |
Names & Taxonomyi
Protein namesi | Recommended name: SPARCAlternative name(s): Basement-membrane protein 40 Short name: BM-40 Osteonectin Short name: ON Secreted protein acidic and rich in cysteine |
Gene namesi | Name:Sparc |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:98373, Sparc |
Subcellular locationi
Extracellular region or secreted
- basement membrane 1 Publication
Note: In or around the basement membrane.
Extracellular region or secreted
- basement membrane Source: UniProtKB-SubCell
- collagen-containing extracellular matrix Source: BHF-UCL
- extracellular matrix Source: MGI
- extracellular space Source: CAFA
Nucleus
- nuclear matrix Source: MGI
- nucleus Source: MGI
Other locations
- cell surface Source: MGI
- cytoplasm Source: MGI
- glutamatergic synapse Source: MGI
- intracellular membrane-bounded organelle Source: MGI
- platelet alpha granule Source: MGI
- platelet alpha granule membrane Source: MGI
- vesicle Source: MGI
Keywords - Cellular componenti
Basement membrane, Extracellular matrix, SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 17 | 1 PublicationAdd BLAST | 17 | |
ChainiPRO_0000020305 | 18 – 302 | SPARCAdd BLAST | 285 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 71 ↔ 82 | PROSITE-ProRule annotation | ||
Disulfide bondi | 76 ↔ 92 | PROSITE-ProRule annotation | ||
Disulfide bondi | 94 ↔ 129 | PROSITE-ProRule annotation | ||
Disulfide bondi | 100 ↔ 122 | PROSITE-ProRule annotation | ||
Disulfide bondi | 111 ↔ 148 | PROSITE-ProRule annotation | ||
Glycosylationi | 115 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Disulfide bondi | 154 ↔ 264 | |||
Disulfide bondi | 272 ↔ 288 |
Post-translational modificationi
N-glycosylated.1 Publication
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
CPTACi | non-CPTAC-3394 |
jPOSTi | P07214 |
PaxDbi | P07214 |
PeptideAtlasi | P07214 |
PRIDEi | P07214 |
PTM databases
GlyConnecti | 2736, 9 N-Linked glycans (1 site) |
GlyGeni | P07214, 1 site |
iPTMneti | P07214 |
PhosphoSitePlusi | P07214 |
Expressioni
Developmental stagei
Expressed at high levels in tissues undergoing morphogenesis, remodeling and wound repair.
Gene expression databases
Bgeei | ENSMUSG00000018593, Expressed in vault of skull and 342 other tissues |
ExpressionAtlasi | P07214, baseline and differential |
Genevisiblei | P07214, MM |
Interactioni
Protein-protein interaction databases
BioGRIDi | 203423, 3 interactors |
IntActi | P07214, 4 interactors |
MINTi | P07214 |
STRINGi | 10090.ENSMUSP00000018737 |
Miscellaneous databases
RNActi | P07214, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 70 – 92 | Follistatin-likeAdd BLAST | 23 | |
Domaini | 88 – 150 | Kazal-likePROSITE-ProRule annotationAdd BLAST | 63 | |
Domaini | 260 – 295 | EF-handAdd BLAST | 36 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 23 – 68 | Asp/Glu-rich (acidic; binds calcium)Add BLAST | 46 |
Sequence similaritiesi
Belongs to the SPARC family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG4004, Eukaryota |
GeneTreei | ENSGT00510000046787 |
InParanoidi | P07214 |
OrthoDBi | 1528521at2759 |
PhylomeDBi | P07214 |
TreeFami | TF319356 |
Family and domain databases
DisProti | DP00052 |
InterProi | View protein in InterPro IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR003645, Fol_N IPR015369, Follistatin/Osteonectin_EGF IPR002350, Kazal_dom IPR036058, Kazal_dom_sf IPR001999, Osteonectin_CS IPR037641, SPARC IPR019577, SPARC/Testican_Ca-bd-dom |
PANTHERi | PTHR13866:SF6, PTHR13866:SF6, 1 hit |
Pfami | View protein in Pfam PF09289, FOLN, 1 hit PF00050, Kazal_1, 1 hit PF10591, SPARC_Ca_bdg, 1 hit |
SMARTi | View protein in SMART SM00274, FOLN, 1 hit SM00280, KAZAL, 1 hit |
SUPFAMi | SSF100895, SSF100895, 1 hit SSF47473, SSF47473, 1 hit |
PROSITEi | View protein in PROSITE PS00018, EF_HAND_1, 1 hit PS51465, KAZAL_2, 1 hit PS00612, OSTEONECTIN_1, 1 hit PS00613, OSTEONECTIN_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
P07214-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRAWIFFLLC LAGRALAAPQ QTEVAEEIVE EETVVEETGV PVGANPVQVE
60 70 80 90 100
MGEFEDGAEE TVEEVVADNP CQNHHCKHGK VCELDESNTP MCVCQDPTSC
110 120 130 140 150
PAPIGEFEKV CSNDNKTFDS SCHFFATKCT LEGTKKGHKL HLDYIGPCKY
160 170 180 190 200
IAPCLDSELT EFPLRMRDWL KNVLVTLYER DEGNNLLTEK QKLRVKKIHE
210 220 230 240 250
NEKRLEAGDH PVELLARDFE KNYNMYIFPV HWQFGQLDQH PIDGYLSHTE
260 270 280 290 300
LAPLRAPLIP MEHCTTRFFE TCDLDNDKYI ALEEWAGCFG IKEQDINKDL
VI
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ5NCU4 | Q5NCU4_MOUSE | Osteonectin | Sparc | 301 | Annotation score: | ||
A0A1L1SSH9 | A0A1L1SSH9_MOUSE | Osteonectin | Sparc | 332 | Annotation score: | ||
Q5NCU3 | Q5NCU3_MOUSE | Osteonectin | Sparc | 172 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X04017 mRNA Translation: CAA27642.1 M20692 , M20684, M20685, M20686, M20687, M20688, M20689, M20690, M20691 Genomic DNA Translation: AAA40125.1 BC004638 mRNA Translation: AAH04638.1 |
CCDSi | CCDS24712.1 |
PIRi | A29227, GEMSN |
RefSeqi | NP_033268.1, NM_009242.5 |
Genome annotation databases
Ensembli | ENSMUST00000018737; ENSMUSP00000018737; ENSMUSG00000018593 ENSMUST00000214685; ENSMUSP00000151000; ENSMUSG00000018593 |
GeneIDi | 20692 |
KEGGi | mmu:20692 |
UCSCi | uc007izh.3, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X04017 mRNA Translation: CAA27642.1 M20692 , M20684, M20685, M20686, M20687, M20688, M20689, M20690, M20691 Genomic DNA Translation: AAA40125.1 BC004638 mRNA Translation: AAH04638.1 |
CCDSi | CCDS24712.1 |
PIRi | A29227, GEMSN |
RefSeqi | NP_033268.1, NM_009242.5 |
3D structure databases
SMRi | P07214 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 203423, 3 interactors |
IntActi | P07214, 4 interactors |
MINTi | P07214 |
STRINGi | 10090.ENSMUSP00000018737 |
PTM databases
GlyConnecti | 2736, 9 N-Linked glycans (1 site) |
GlyGeni | P07214, 1 site |
iPTMneti | P07214 |
PhosphoSitePlusi | P07214 |
Proteomic databases
CPTACi | non-CPTAC-3394 |
jPOSTi | P07214 |
PaxDbi | P07214 |
PeptideAtlasi | P07214 |
PRIDEi | P07214 |
Protocols and materials databases
Antibodypediai | 878, 758 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000018737; ENSMUSP00000018737; ENSMUSG00000018593 ENSMUST00000214685; ENSMUSP00000151000; ENSMUSG00000018593 |
GeneIDi | 20692 |
KEGGi | mmu:20692 |
UCSCi | uc007izh.3, mouse |
Organism-specific databases
CTDi | 6678 |
MGIi | MGI:98373, Sparc |
Phylogenomic databases
eggNOGi | KOG4004, Eukaryota |
GeneTreei | ENSGT00510000046787 |
InParanoidi | P07214 |
OrthoDBi | 1528521at2759 |
PhylomeDBi | P07214 |
TreeFami | TF319356 |
Enzyme and pathway databases
Reactomei | R-MMU-114608, Platelet degranulation R-MMU-3000178, ECM proteoglycans R-MMU-3000497, Scavenging by Class H Receptors |
Miscellaneous databases
BioGRID-ORCSi | 20692, 1 hit in 19 CRISPR screens |
ChiTaRSi | Sparc, mouse |
PROi | PR:P07214 |
RNActi | P07214, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000018593, Expressed in vault of skull and 342 other tissues |
ExpressionAtlasi | P07214, baseline and differential |
Genevisiblei | P07214, MM |
Family and domain databases
DisProti | DP00052 |
InterProi | View protein in InterPro IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR003645, Fol_N IPR015369, Follistatin/Osteonectin_EGF IPR002350, Kazal_dom IPR036058, Kazal_dom_sf IPR001999, Osteonectin_CS IPR037641, SPARC IPR019577, SPARC/Testican_Ca-bd-dom |
PANTHERi | PTHR13866:SF6, PTHR13866:SF6, 1 hit |
Pfami | View protein in Pfam PF09289, FOLN, 1 hit PF00050, Kazal_1, 1 hit PF10591, SPARC_Ca_bdg, 1 hit |
SMARTi | View protein in SMART SM00274, FOLN, 1 hit SM00280, KAZAL, 1 hit |
SUPFAMi | SSF100895, SSF100895, 1 hit SSF47473, SSF47473, 1 hit |
PROSITEi | View protein in PROSITE PS00018, EF_HAND_1, 1 hit PS51465, KAZAL_2, 1 hit PS00612, OSTEONECTIN_1, 1 hit PS00613, OSTEONECTIN_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SPRC_MOUSE | |
Accessioni | P07214Primary (citable) accession number: P07214 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1988 |
Last sequence update: | April 1, 1988 | |
Last modified: | February 10, 2021 | |
This is version 185 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families