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Protein

Pullulanase

Gene

pulA

Organism
Klebsiella pneumoniae
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of (1->6)-alpha-D-glucosidic linkages in pullulan, amylopectin and glycogen, and in the alpha- and beta-limit dextrins of amylopectin and glycogen.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei684NucleophileBy similarity1
Active sitei713Proton donorBy similarity1
Sitei841Transition state stabilizerBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase

Protein family/group databases

CAZyiCBM41 Carbohydrate-Binding Module Family 41
CBM48 Carbohydrate-Binding Module Family 48
GH13 Glycoside Hydrolase Family 13

Names & Taxonomyi

Protein namesi
Recommended name:
Pullulanase (EC:3.2.1.41)
Alternative name(s):
Alpha-dextrin endo-1,6-alpha-glucosidase
Pullulan 6-glucanohydrolase
Gene namesi
Name:pulA
OrganismiKlebsiella pneumoniae
Taxonomic identifieri573 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeKlebsiella

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19PROSITE-ProRule annotationAdd BLAST19
ChainiPRO_000000142720 – 1090PullulanaseAdd BLAST1071

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi20N-palmitoyl cysteinePROSITE-ProRule annotation1
Lipidationi20S-diacylglycerol cysteinePROSITE-ProRule annotation1

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

PRIDEiP07206

Interactioni

Subunit structurei

Homotrimer.

Structurei

Secondary structure

11090
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP07206
SMRiP07206
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07206

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Signal

Family and domain databases

CDDicd10315 CBM41_pullulanase, 1 hit
Gene3Di2.60.40.10, 1 hit
2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR013784 Carb-bd-like_fold
IPR005323 CBM41_pullulanase
IPR006047 Glyco_hydro_13_cat_dom
IPR004193 Glyco_hydro_13_N
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
IPR013783 Ig-like_fold
IPR014756 Ig_E-set
IPR011839 Pullul_strch
IPR024561 Pullul_strch_C
PfamiView protein in Pfam
PF02922 CBM_48, 1 hit
PF11852 DUF3372, 1 hit
PF03714 PUD, 1 hit
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SUPFAMiSSF49452 SSF49452, 1 hit
SSF51445 SSF51445, 1 hit
SSF81296 SSF81296, 2 hits
TIGRFAMsiTIGR02103 pullul_strch, 1 hit
PROSITEiView protein in PROSITE
PS51257 PROKAR_LIPOPROTEIN, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07206-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLRYTRNALV LGSLVLLSGC DNGSSSSSSG NPDTPDNQDV VVRLPDVAVP
60 70 80 90 100
GEAVTAVENQ AVIHLVDIAG ITSSSAADYS SKNLYLWNNE TCDALSAPVA
110 120 130 140 150
DWNDVSTTPS GSDKYGPYWV IPLNKESGCI NVIVRDGTDK LIDSDLRVAF
160 170 180 190 200
GDFTDRTVSV IAGNSAVYDS RADAFRAAFG VALAEAHWVD KNTLLWPGGQ
210 220 230 240 250
DKPIVRLYYS HSSKVAADGE GKFTDRYLKL TPTTVSQQVS MRFPHLSSYA
260 270 280 290 300
AFKLPDNANV DELLQGETVA IAAAEDGILI SATQVQTAGV LDDAYAEAAE
310 320 330 340 350
ALSYGAQLAD GGVTFRVWAP TAQQVDVVVY SADKKVIGSH PMTRDSASGA
360 370 380 390 400
WSWQGGSDLK GAFYRYAMTV YHPQSRKVEQ YEVTDPYAHS LSTNSEYSQV
410 420 430 440 450
VDLNDSALKP DGWDNLTMPH AQKTKADLAK MTIHESHIRD LSAWDQTVPA
460 470 480 490 500
ELRGKYLALT AGDSNMVQHL KTLSASGVTH VELLPVFDLA TVNEFSDKVA
510 520 530 540 550
DIQQPFSRLC EVNSAVKSSE FAGYCDSGST VEEVLNQLKQ SDSQDNPQVQ
560 570 580 590 600
ALNTLVAQTD SYNWGYDPFH YTVPEGSYAT DPEGTTRIKE FRTMIQAIKQ
610 620 630 640 650
DLGMNVIMDV VYNHTNAAGP TDRTSVLDKI VPWYYQRLNE TTGSVESATC
660 670 680 690 700
CSDSAPEHRM FAKLIADSLA VWTTDYKIDG FRFDLMGYHP KAQILSAWER
710 720 730 740 750
IKALNPDIYF FGEGWDSNQS DRFEIASQIN LKGTGIGTFS DRLRDSVRGG
760 770 780 790 800
GPFDSGDALR QNQGIGSGAG VLPNELASLS DDQVRHLADL TRLGMAGNLA
810 820 830 840 850
DFVMIDKDGA AKKGSEIDYN GAPGGYAADP TEVVNYVSKH DNQTLWDMIS
860 870 880 890 900
YKASQEADLA TRVRMQAVSL ATVMLGQGIA FDQQGSELLR SKSFTRDSYD
910 920 930 940 950
SGDWFNRVDY SLQDNNYNVG MPRISDDGSN YEVITRVKEM VATPGEAELK
960 970 980 990 1000
QMTAFYQELT ELRKSSPLFT LGDGSAVMKR VDFRNTGSDQ QAGLLVMTVD
1010 1020 1030 1040 1050
DGMKAGASLD SRLDGLVVAI NAAPESRTLN EFAGETLQLS AIQQTAGENS
1060 1070 1080 1090
LANGVQIAAD GTVTLPAWSV AVLELPQGEA QGAGLPVSSK
Length:1,090
Mass (Da):118,098
Last modified:April 1, 1990 - v2
Checksum:i240AE7DFB3FF1BD6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti6R → C in AAA25087 (PubMed:3902792).Curated1
Sequence conflicti10V → F in AAA25087 (PubMed:3902792).Curated1
Sequence conflicti15V → I in AAA25087 (PubMed:3902792).Curated1
Sequence conflicti23G → S in AAA25087 (PubMed:3902792).Curated1
Sequence conflicti31N → S in AAA25087 (PubMed:3902792).Curated1
Sequence conflicti34T → N in AAA25087 (PubMed:3902792).Curated1
Sequence conflicti36D → DGNP in AAA25087 (PubMed:3902792).Curated1
Sequence conflicti55 – 58TAVE → MATA in AAA25087 (PubMed:3902792).Curated4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52181 Genomic DNA Translation: CAA36431.1
M12503 Genomic DNA Translation: AAA25087.2
M29097 Genomic DNA Translation: AAA61976.1
PIRiS11823
S38801

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52181 Genomic DNA Translation: CAA36431.1
M12503 Genomic DNA Translation: AAA25087.2
M29097 Genomic DNA Translation: AAA61976.1
PIRiS11823
S38801

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2YOCX-ray2.88A/B21-1089[»]
ProteinModelPortaliP07206
SMRiP07206
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiCBM41 Carbohydrate-Binding Module Family 41
CBM48 Carbohydrate-Binding Module Family 48
GH13 Glycoside Hydrolase Family 13

Proteomic databases

PRIDEiP07206

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP07206

Family and domain databases

CDDicd10315 CBM41_pullulanase, 1 hit
Gene3Di2.60.40.10, 1 hit
2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR013784 Carb-bd-like_fold
IPR005323 CBM41_pullulanase
IPR006047 Glyco_hydro_13_cat_dom
IPR004193 Glyco_hydro_13_N
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
IPR013783 Ig-like_fold
IPR014756 Ig_E-set
IPR011839 Pullul_strch
IPR024561 Pullul_strch_C
PfamiView protein in Pfam
PF02922 CBM_48, 1 hit
PF11852 DUF3372, 1 hit
PF03714 PUD, 1 hit
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SUPFAMiSSF49452 SSF49452, 1 hit
SSF51445 SSF51445, 1 hit
SSF81296 SSF81296, 2 hits
TIGRFAMsiTIGR02103 pullul_strch, 1 hit
PROSITEiView protein in PROSITE
PS51257 PROKAR_LIPOPROTEIN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPULA_KLEPN
AccessioniPrimary (citable) accession number: P07206
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1990
Last modified: May 23, 2018
This is version 136 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
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