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Protein

Phosphoglycerate kinase 2

Gene

PGK2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Essential for sperm motility and male fertility (PubMed:26677959). Not required for the completion of spermatogenesis (By similarity).By similarity1 Publication

Catalytic activityi

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.

Pathwayi: glycolysis

This protein is involved in step 2 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase, testis-specific (GAPDHS), Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Glyceraldehyde-3-phosphate dehydrogenase (epd), Glyceraldehyde-3-phosphate dehydrogenase, Glyceraldehyde-3-phosphate dehydrogenase (HEL-S-278), Glyceraldehyde-3-phosphate dehydrogenase (HEL-S-162eP)
  2. Phosphoglycerate kinase 1 (PGK1), Phosphoglycerate kinase 2 (PGK2), Phosphoglycerate kinase, Phosphoglycerate kinase, Phosphoglycerate kinase, Phosphoglycerate kinase (pgk), Phosphoglycerate kinase, Phosphoglycerate kinase (HEL-S-68p), Phosphoglycerate kinase (HEL-S-272), Phosphoglycerate kinase
  3. no protein annotated in this organism
  4. Gamma-enolase (ENO2), Alpha-enolase (ENO1), Enolase 4 (ENO4), Beta-enolase (ENO3)
  5. Pyruvate kinase PKM (PKM), Pyruvate kinase PKLR (PKLR), Pyruvate kinase (PKM), Pyruvate kinase (PKM2), Pyruvate kinase (PKM), Pyruvate kinase (PKM2), Pyruvate kinase, Pyruvate kinase (PKM), Pyruvate kinase (PKM2), Pyruvate kinase, Pyruvate kinase (pyk), Pyruvate kinase (PKM), Pyruvate kinase, Pyruvate kinase (HEL-S-30), Pyruvate kinase, Pyruvate kinase (PKM2)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei39SubstrateBy similarity1
Binding sitei123SubstrateBy similarity1
Binding sitei171SubstrateBy similarity1
Binding sitei220ATPBy similarity1
Binding sitei313ATP; via carbonyl oxygenBy similarity1
Binding sitei344ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi373 – 376ATPBy similarity4

GO - Molecular functioni

  • ADP binding Source: GO_Central
  • ATP binding Source: UniProtKB
  • phosphoglycerate kinase activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processGlycolysis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:HS10215-MONOMER
ReactomeiR-HSA-70171 Glycolysis
R-HSA-70263 Gluconeogenesis
UniPathwayi
UPA00109;UER00185

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoglycerate kinase 2 (EC:2.7.2.3)
Alternative name(s):
Phosphoglycerate kinase, testis specific
Gene namesi
Name:PGK2
Synonyms:PGKB
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiHostDB:ENSG00000170950.5
HGNCiHGNC:8898 PGK2
MIMi172270 gene
neXtProtiNX_P07205

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi5232
OpenTargetsiENSG00000170950
PharmGKBiPA33237

Chemistry databases

ChEMBLiCHEMBL2096677

Polymorphism and mutation databases

BioMutaiPGK2
DMDMi21264485

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001458322 – 417Phosphoglycerate kinase 2Add BLAST416

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei2PhosphoserineBy similarity1
Modified residuei4PhosphoserineBy similarity1
Modified residuei11N6-acetyllysineBy similarity1
Modified residuei48N6-acetyllysineBy similarity1
Modified residuei75N6-acetyllysineBy similarity1
Modified residuei86N6-acetyllysineBy similarity1
Modified residuei97N6-acetyllysineBy similarity1
Modified residuei131N6-acetyllysineBy similarity1
Modified residuei146N6-acetyllysineBy similarity1
Modified residuei196PhosphotyrosineBy similarity1
Modified residuei199N6-acetyllysineBy similarity1
Modified residuei267N6-acetyllysineBy similarity1
Modified residuei291N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP07205
MaxQBiP07205
PaxDbiP07205
PeptideAtlasiP07205
PRIDEiP07205
ProteomicsDBi51974

2D gel databases

UCD-2DPAGEiP07205

PTM databases

iPTMnetiP07205
PhosphoSitePlusiP07205
SwissPalmiP07205

Expressioni

Tissue specificityi

Mainly found in round spermatids. Localized on the principle piece in the sperm (at protein level). Testis-specific. Expression significantly decreased in the testis of elderly men.1 Publication

Gene expression databases

BgeeiENSG00000170950 Expressed in 47 organ(s), highest expression level in sperm
CleanExiHS_PGK2
ExpressionAtlasiP07205 baseline and differential
GenevisibleiP07205 HS

Organism-specific databases

HPAiHPA045385
HPA073644
HPA073656

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi111253, 32 interactors
STRINGi9606.ENSP00000305995

Structurei

3D structure databases

ProteinModelPortaliP07205
SMRiP07205
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni24 – 26Substrate bindingBy similarity3
Regioni63 – 66Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the phosphoglycerate kinase family.Curated

Phylogenomic databases

eggNOGiKOG1367 Eukaryota
COG0126 LUCA
GeneTreeiENSGT00390000008820
HOGENOMiHOG000227107
HOVERGENiHBG008177
InParanoidiP07205
KOiK00927
OMAiAEPDKIE
OrthoDBiEOG091G08S6
PhylomeDBiP07205
TreeFamiTF300489

Family and domain databases

CDDicd00318 Phosphoglycerate_kinase, 1 hit
Gene3Di3.40.50.1260, 3 hits
HAMAPiMF_00145 Phosphoglyc_kinase, 1 hit
InterProiView protein in InterPro
IPR001576 Phosphoglycerate_kinase
IPR015911 Phosphoglycerate_kinase_CS
IPR015824 Phosphoglycerate_kinase_N
IPR036043 Phosphoglycerate_kinase_sf
PANTHERiPTHR11406 PTHR11406, 1 hit
PfamiView protein in Pfam
PF00162 PGK, 1 hit
PIRSFiPIRSF000724 Pgk, 1 hit
PRINTSiPR00477 PHGLYCKINASE
SUPFAMiSSF53748 SSF53748, 1 hit
PROSITEiView protein in PROSITE
PS00111 PGLYCERATE_KINASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07205-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSLSKKLTLD KLDVRGKRVI MRVDFNVPMK KNQITNNQRI KASIPSIKYC
60 70 80 90 100
LDNGAKAVVL MSHLGRPDGV PMPDKYSLAP VAVELKSLLG KDVLFLKDCV
110 120 130 140 150
GAEVEKACAN PAPGSVILLE NLRFHVEEEG KGQDPSGKKI KAEPDKIEAF
160 170 180 190 200
RASLSKLGDV YVNDAFGTAH RAHSSMVGVN LPHKASGFLM KKELDYFAKA
210 220 230 240 250
LENPVRPFLA ILGGAKVADK IQLIKNMLDK VNEMIIGGGM AYTFLKVLNN
260 270 280 290 300
MEIGASLFDE EGAKIVKDIM AKAQKNGVRI TFPVDFVTGD KFDENAQVGK
310 320 330 340 350
ATVASGISPG WMGLDCGPES NKNHAQVVAQ ARLIVWNGPL GVFEWDAFAK
360 370 380 390 400
GTKALMDEIV KATSKGCITV IGGGDTATCC AKWNTEDKVS HVSTGGGASL
410
ELLEGKILPG VEALSNM
Length:417
Mass (Da):44,796
Last modified:January 23, 2007 - v3
Checksum:i0CD5C71C2D3A9272
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti396G → R in CAA28872 (PubMed:3453121).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05246 Genomic DNA Translation: CAA28872.1
AK312770 mRNA Translation: BAG35635.1
AL121974 Genomic DNA No translation available.
BC038843 mRNA Translation: AAH38843.1
CCDSiCCDS4930.1
PIRiA24030
A27816
RefSeqiNP_620061.2, NM_138733.4
UniGeneiHs.367727

Genome annotation databases

EnsembliENST00000304801; ENSP00000305995; ENSG00000170950
GeneIDi5232
KEGGihsa:5232
UCSCiuc003ozu.4 human

Similar proteinsi

Cross-referencesi

Web resourcesi

Wikipedia

Phosphoglycerate kinase entry

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05246 Genomic DNA Translation: CAA28872.1
AK312770 mRNA Translation: BAG35635.1
AL121974 Genomic DNA No translation available.
BC038843 mRNA Translation: AAH38843.1
CCDSiCCDS4930.1
PIRiA24030
A27816
RefSeqiNP_620061.2, NM_138733.4
UniGeneiHs.367727

3D structure databases

ProteinModelPortaliP07205
SMRiP07205
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111253, 32 interactors
STRINGi9606.ENSP00000305995

Chemistry databases

ChEMBLiCHEMBL2096677

PTM databases

iPTMnetiP07205
PhosphoSitePlusiP07205
SwissPalmiP07205

Polymorphism and mutation databases

BioMutaiPGK2
DMDMi21264485

2D gel databases

UCD-2DPAGEiP07205

Proteomic databases

EPDiP07205
MaxQBiP07205
PaxDbiP07205
PeptideAtlasiP07205
PRIDEiP07205
ProteomicsDBi51974

Protocols and materials databases

DNASUi5232
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000304801; ENSP00000305995; ENSG00000170950
GeneIDi5232
KEGGihsa:5232
UCSCiuc003ozu.4 human

Organism-specific databases

CTDi5232
DisGeNETi5232
EuPathDBiHostDB:ENSG00000170950.5
GeneCardsiPGK2
HGNCiHGNC:8898 PGK2
HPAiHPA045385
HPA073644
HPA073656
MIMi172270 gene
neXtProtiNX_P07205
OpenTargetsiENSG00000170950
PharmGKBiPA33237
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1367 Eukaryota
COG0126 LUCA
GeneTreeiENSGT00390000008820
HOGENOMiHOG000227107
HOVERGENiHBG008177
InParanoidiP07205
KOiK00927
OMAiAEPDKIE
OrthoDBiEOG091G08S6
PhylomeDBiP07205
TreeFamiTF300489

Enzyme and pathway databases

UniPathwayi
UPA00109;UER00185

BioCyciMetaCyc:HS10215-MONOMER
ReactomeiR-HSA-70171 Glycolysis
R-HSA-70263 Gluconeogenesis

Miscellaneous databases

GenomeRNAii5232
PROiPR:P07205
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000170950 Expressed in 47 organ(s), highest expression level in sperm
CleanExiHS_PGK2
ExpressionAtlasiP07205 baseline and differential
GenevisibleiP07205 HS

Family and domain databases

CDDicd00318 Phosphoglycerate_kinase, 1 hit
Gene3Di3.40.50.1260, 3 hits
HAMAPiMF_00145 Phosphoglyc_kinase, 1 hit
InterProiView protein in InterPro
IPR001576 Phosphoglycerate_kinase
IPR015911 Phosphoglycerate_kinase_CS
IPR015824 Phosphoglycerate_kinase_N
IPR036043 Phosphoglycerate_kinase_sf
PANTHERiPTHR11406 PTHR11406, 1 hit
PfamiView protein in Pfam
PF00162 PGK, 1 hit
PIRSFiPIRSF000724 Pgk, 1 hit
PRINTSiPR00477 PHGLYCKINASE
SUPFAMiSSF53748 SSF53748, 1 hit
PROSITEiView protein in PROSITE
PS00111 PGLYCERATE_KINASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPGK2_HUMAN
AccessioniPrimary (citable) accession number: P07205
Secondary accession number(s): B2R6Y8, Q9H107
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 170 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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