UniProtKB - P07203 (GPX1_HUMAN)
Protein
Glutathione peroxidase 1
Gene
GPX1
Organism
Homo sapiens (Human)
Status
Functioni
Protects the hemoglobin in erythrocytes from oxidative breakdown. In platelets, plays a crucial role of glutathione peroxidase in the arachidonic acid metabolism (PubMed:11115402).1 Publication
Catalytic activityi
- EC:1.11.1.91 PublicationThis reaction proceeds in the forward1 Publication direction.
- (12S)-hydroperoxy-(5Z,8Z,10E,14Z)-eicosatetraenoate + 2 glutathione = (12S)-hydroxy-(5Z,8Z,10E,14Z)-eicosatetraenoate + glutathione disulfide + H2O1 PublicationThis reaction proceeds in the forward1 Publication direction.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 49 | 1 | ||
Sitei | 49 | Subject to oxidation and hydroselenide loss to dehydroalanineBy similarity | 1 |
GO - Molecular functioni
- glutathione peroxidase activity Source: BHF-UCL
- peroxidase activity Source: GO_Central
- phospholipid-hydroperoxide glutathione peroxidase activity Source: UniProtKB
- SH3 domain binding Source: BHF-UCL
GO - Biological processi
- angiogenesis involved in wound healing Source: Ensembl
- arachidonic acid metabolic process Source: UniProtKB
- blood vessel endothelial cell migration Source: Ensembl
- cell redox homeostasis Source: BHF-UCL
- cellular response to oxidative stress Source: Reactome
- endothelial cell development Source: Ensembl
- fat cell differentiation Source: Ensembl
- glutathione metabolic process Source: BHF-UCL
- heart contraction Source: BHF-UCL
- hydrogen peroxide catabolic process Source: BHF-UCL
- interaction with symbiont Source: Ensembl
- lipoxygenase pathway Source: UniProtKB
- myoblast proliferation Source: Ensembl
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: BHF-UCL
- negative regulation of extrinsic apoptotic signaling pathway via death domain receptors Source: BHF-UCL
- negative regulation of inflammatory response to antigenic stimulus Source: Ensembl
- negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway Source: Ensembl
- negative regulation of release of cytochrome c from mitochondria Source: BHF-UCL
- positive regulation of protein kinase B signaling Source: Ensembl
- positive regulation of supramolecular fiber organization Source: ParkinsonsUK-UCL
- protein oxidation Source: Ensembl
- purine nucleotide catabolic process Source: Reactome
- regulation of gene expression, epigenetic Source: BHF-UCL
- regulation of mammary gland epithelial cell proliferation Source: BHF-UCL
- regulation of proteasomal protein catabolic process Source: BHF-UCL
- response to gamma radiation Source: Ensembl
- response to hydrogen peroxide Source: BHF-UCL
- response to hydroperoxide Source: Ensembl
- response to selenium ion Source: BHF-UCL
- response to symbiotic bacterium Source: Ensembl
- response to xenobiotic stimulus Source: Ensembl
- sensory perception of sound Source: Ensembl
- skeletal muscle fiber development Source: Ensembl
- skeletal muscle tissue regeneration Source: Ensembl
- temperature homeostasis Source: Ensembl
- triglyceride metabolic process Source: Ensembl
- UV protection Source: BHF-UCL
- vasodilation Source: Ensembl
Keywordsi
Molecular function | Oxidoreductase, Peroxidase |
Enzyme and pathway databases
BioCyci | MetaCyc:HS00019-MONOMER |
BRENDAi | 1.11.1.9, 2681 |
PathwayCommonsi | P07203 |
Reactomei | R-HSA-2142688, Synthesis of 5-eicosatetraenoic acids R-HSA-2142712, Synthesis of 12-eicosatetraenoic acid derivatives R-HSA-2142770, Synthesis of 15-eicosatetraenoic acid derivatives R-HSA-3299685, Detoxification of Reactive Oxygen Species R-HSA-74259, Purine catabolism |
SIGNORi | P07203 |
Protein family/group databases
PeroxiBasei | 3600, HsGPx01-A |
Chemistry databases
SwissLipidsi | SLP:000001632 |
Names & Taxonomyi
Protein namesi | Recommended name: Glutathione peroxidase 1Curated (EC:1.11.1.91 Publication)Short name: GPx-11 Publication Short name: GSHPx-1 Alternative name(s): Cellular glutathione peroxidase |
Gene namesi | Name:GPX1Imported |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:4553, GPX1 |
MIMi | 138320, gene+phenotype |
neXtProti | NX_P07203 |
VEuPathDBi | HostDB:ENSG00000233276.3 |
Subcellular locationi
Other locations
Cytosol
- cytosol Source: GO_Central
Mitochondrion
- mitochondrial matrix Source: Reactome
- mitochondrion Source: BHF-UCL
Other locations
- cytoplasm Source: BHF-UCL
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Organism-specific databases
DisGeNETi | 2876 |
MalaCardsi | GPX1 |
MIMi | 138320, gene+phenotype |
OpenTargetsi | ENSG00000233276 |
PharmGKBi | PA28949 |
Miscellaneous databases
Pharosi | P07203, Tbio |
Chemistry databases
ChEMBLi | CHEMBL2163186 |
DrugBanki | DB09061, Cannabidiol DB00143, Glutathione DB03310, Glutathione disulfide DB11091, Hydrogen peroxide DB14009, Medical Cannabis DB14011, Nabiximols DB09221, Polaprezinc DB11127, Selenious acid DB11590, Thimerosal |
Genetic variation databases
BioMutai | GPX1 |
DMDMi | 311033481 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000066610 | 1 – 203 | Glutathione peroxidase 1Add BLAST | 203 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 34 | PhosphoserineBy similarity | 1 | |
Modified residuei | 88 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 88 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 114 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 114 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 148 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 148 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 197 | PhosphoserineBy similarity | 1 | |
Modified residuei | 201 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
During periods of oxidative stress, Sec-49 may react with a superoxide radical, irreversibly lose hydroselenide and be converted to dehydroalanine.By similarity
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | P07203 |
jPOSTi | P07203 |
MassIVEi | P07203 |
PaxDbi | P07203 |
PeptideAtlasi | P07203 |
PRIDEi | P07203 |
ProteomicsDBi | 19069 51972 [P07203-1] |
2D gel databases
OGPi | P07203 |
SWISS-2DPAGEi | P07203 |
PTM databases
iPTMneti | P07203 |
MetOSitei | P07203 |
PhosphoSitePlusi | P07203 |
SwissPalmi | P07203 |
Expressioni
Tissue specificityi
Expressed in platelets (at protein level).1 Publication
Gene expression databases
Bgeei | ENSG00000233276, Expressed in monocyte and 113 other tissues |
ExpressionAtlasi | P07203, baseline and differential |
Genevisiblei | P07203, HS |
Interactioni
Subunit structurei
Homotetramer.
Interacts with MIEN1.
GO - Molecular functioni
- SH3 domain binding Source: BHF-UCL
Protein-protein interaction databases
BioGRIDi | 109134, 43 interactors |
CORUMi | P07203 |
IntActi | P07203, 15 interactors |
MINTi | P07203 |
STRINGi | 9606.ENSP00000407375 |
Chemistry databases
BindingDBi | P07203 |
Miscellaneous databases
RNActi | P07203, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P07203 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P07203 |
Family & Domainsi
Sequence similaritiesi
Belongs to the glutathione peroxidase family.Curated
Phylogenomic databases
eggNOGi | KOG1651, Eukaryota |
GeneTreei | ENSGT00940000156150 |
HOGENOMi | CLU_029507_2_0_1 |
InParanoidi | P07203 |
OMAi | MNDLQRR |
PhylomeDBi | P07203 |
TreeFami | TF105318 |
Family and domain databases
CDDi | cd00340, GSH_Peroxidase, 1 hit |
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR000889, Glutathione_peroxidase IPR029759, GPX_AS IPR029760, GPX_CS IPR036249, Thioredoxin-like_sf |
PANTHERi | PTHR11592, PTHR11592, 1 hit |
Pfami | View protein in Pfam PF00255, GSHPx, 1 hit |
PIRSFi | PIRSF000303, Glutathion_perox, 1 hit |
PRINTSi | PR01011, GLUTPROXDASE |
SUPFAMi | SSF52833, SSF52833, 1 hit |
PROSITEi | View protein in PROSITE PS00460, GLUTATHIONE_PEROXID_1, 1 hit PS00763, GLUTATHIONE_PEROXID_2, 1 hit PS51355, GLUTATHIONE_PEROXID_3, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: P07203-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MCAARLAAAA AAAQSVYAFS ARPLAGGEPV SLGSLRGKVL LIENVASLUG
60 70 80 90 100
TTVRDYTQMN ELQRRLGPRG LVVLGFPCNQ FGHQENAKNE EILNSLKYVR
110 120 130 140 150
PGGGFEPNFM LFEKCEVNGA GAHPLFAFLR EALPAPSDDA TALMTDPKLI
160 170 180 190 200
TWSPVCRNDV AWNFEKFLVG PDGVPLRRYS RRFQTIDIEP DIEALLSQGP
SCA
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A087WUQ6 | A0A087WUQ6_HUMAN | Glutathione peroxidase | GPX1 | 202 | Annotation score: | ||
A0A2R8Y6B6 | A0A2R8Y6B6_HUMAN | Glutathione peroxidase 1 | GPX1 | 157 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 93 | L → Q in CAA31992 (PubMed:2955287).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_020912 | 5 | R → P1 PublicationCorresponds to variant dbSNP:rs8179169Ensembl. | 1 | |
Natural variantiVAR_020913 | 7 – 8 | Missing 5 Publications | 2 | |
Natural variantiVAR_020914 | 8 | Missing 3 Publications | 1 | |
Natural variantiVAR_020915 | 194 | A → T1 PublicationCorresponds to variant dbSNP:rs6446261Ensembl. | 1 | |
Natural variantiVAR_007904 | 200 | P → L4 PublicationsCorresponds to variant dbSNP:rs1050450EnsemblClinVar. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_047369 | 85 – 98 | ENAKN…NSLKY → VRRAERGGAGADVQ in isoform 2. CuratedAdd BLAST | 14 | |
Alternative sequenceiVSP_047370 | 99 – 203 | Missing in isoform 2. CuratedAdd BLAST | 105 |
Non-standard residue
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Non-standard residuei | 49 | Selenocysteine | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y00433 mRNA Translation: CAA68491.1 Y00483 Genomic DNA Translation: CAB37833.1 X13709 mRNA Translation: CAA31992.1 X13710 mRNA Translation: CAA31993.1 M21304 mRNA Translation: AAA75389.2 M83094 Genomic DNA Translation: AAA67540.2 AY327818 Genomic DNA Translation: AAP80181.1 AC121247 Genomic DNA No translation available. BC000742 mRNA Translation: AAH00742.3 |
CCDSi | CCDS43091.1 [P07203-1] CCDS54582.1 [P07203-2] |
PIRi | A42152, OPHUE |
RefSeqi | NP_000572.2, NM_000581.3 [P07203-1] |
Genome annotation databases
Ensembli | ENST00000419349; ENSP00000391316; ENSG00000233276 [P07203-2] ENST00000419783; ENSP00000407375; ENSG00000233276 [P07203-1] |
GeneIDi | 2876 |
KEGGi | hsa:2876 |
UCSCi | uc021wxw.2, human [P07203-1] |
Keywords - Coding sequence diversityi
Alternative splicing, SelenocysteineSimilar proteinsi
Cross-referencesi
Web resourcesi
NIEHS-SNPs |
Wikipedia Glutathione peroxidase entry |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y00433 mRNA Translation: CAA68491.1 Y00483 Genomic DNA Translation: CAB37833.1 X13709 mRNA Translation: CAA31992.1 X13710 mRNA Translation: CAA31993.1 M21304 mRNA Translation: AAA75389.2 M83094 Genomic DNA Translation: AAA67540.2 AY327818 Genomic DNA Translation: AAP80181.1 AC121247 Genomic DNA No translation available. BC000742 mRNA Translation: AAH00742.3 |
CCDSi | CCDS43091.1 [P07203-1] CCDS54582.1 [P07203-2] |
PIRi | A42152, OPHUE |
RefSeqi | NP_000572.2, NM_000581.3 [P07203-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2F8A | X-ray | 1.50 | A/B | 14-198 | [»] | |
SMRi | P07203 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 109134, 43 interactors |
CORUMi | P07203 |
IntActi | P07203, 15 interactors |
MINTi | P07203 |
STRINGi | 9606.ENSP00000407375 |
Chemistry databases
BindingDBi | P07203 |
ChEMBLi | CHEMBL2163186 |
DrugBanki | DB09061, Cannabidiol DB00143, Glutathione DB03310, Glutathione disulfide DB11091, Hydrogen peroxide DB14009, Medical Cannabis DB14011, Nabiximols DB09221, Polaprezinc DB11127, Selenious acid DB11590, Thimerosal |
SwissLipidsi | SLP:000001632 |
Protein family/group databases
PeroxiBasei | 3600, HsGPx01-A |
PTM databases
iPTMneti | P07203 |
MetOSitei | P07203 |
PhosphoSitePlusi | P07203 |
SwissPalmi | P07203 |
Genetic variation databases
BioMutai | GPX1 |
DMDMi | 311033481 |
2D gel databases
OGPi | P07203 |
SWISS-2DPAGEi | P07203 |
Proteomic databases
EPDi | P07203 |
jPOSTi | P07203 |
MassIVEi | P07203 |
PaxDbi | P07203 |
PeptideAtlasi | P07203 |
PRIDEi | P07203 |
ProteomicsDBi | 19069 51972 [P07203-1] |
Protocols and materials databases
Antibodypediai | 39716, 521 antibodies |
DNASUi | 2876 |
Genome annotation databases
Ensembli | ENST00000419349; ENSP00000391316; ENSG00000233276 [P07203-2] ENST00000419783; ENSP00000407375; ENSG00000233276 [P07203-1] |
GeneIDi | 2876 |
KEGGi | hsa:2876 |
UCSCi | uc021wxw.2, human [P07203-1] |
Organism-specific databases
CTDi | 2876 |
DisGeNETi | 2876 |
GeneCardsi | GPX1 |
HGNCi | HGNC:4553, GPX1 |
MalaCardsi | GPX1 |
MIMi | 138320, gene+phenotype |
neXtProti | NX_P07203 |
OpenTargetsi | ENSG00000233276 |
PharmGKBi | PA28949 |
VEuPathDBi | HostDB:ENSG00000233276.3 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1651, Eukaryota |
GeneTreei | ENSGT00940000156150 |
HOGENOMi | CLU_029507_2_0_1 |
InParanoidi | P07203 |
OMAi | MNDLQRR |
PhylomeDBi | P07203 |
TreeFami | TF105318 |
Enzyme and pathway databases
BioCyci | MetaCyc:HS00019-MONOMER |
BRENDAi | 1.11.1.9, 2681 |
PathwayCommonsi | P07203 |
Reactomei | R-HSA-2142688, Synthesis of 5-eicosatetraenoic acids R-HSA-2142712, Synthesis of 12-eicosatetraenoic acid derivatives R-HSA-2142770, Synthesis of 15-eicosatetraenoic acid derivatives R-HSA-3299685, Detoxification of Reactive Oxygen Species R-HSA-74259, Purine catabolism |
SIGNORi | P07203 |
Miscellaneous databases
BioGRID-ORCSi | 2876, 3 hits in 873 CRISPR screens |
ChiTaRSi | GPX1, human |
EvolutionaryTracei | P07203 |
GeneWikii | GPX1 |
GenomeRNAii | 2876 |
Pharosi | P07203, Tbio |
PROi | PR:P07203 |
RNActi | P07203, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000233276, Expressed in monocyte and 113 other tissues |
ExpressionAtlasi | P07203, baseline and differential |
Genevisiblei | P07203, HS |
Family and domain databases
CDDi | cd00340, GSH_Peroxidase, 1 hit |
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR000889, Glutathione_peroxidase IPR029759, GPX_AS IPR029760, GPX_CS IPR036249, Thioredoxin-like_sf |
PANTHERi | PTHR11592, PTHR11592, 1 hit |
Pfami | View protein in Pfam PF00255, GSHPx, 1 hit |
PIRSFi | PIRSF000303, Glutathion_perox, 1 hit |
PRINTSi | PR01011, GLUTPROXDASE |
SUPFAMi | SSF52833, SSF52833, 1 hit |
PROSITEi | View protein in PROSITE PS00460, GLUTATHIONE_PEROXID_1, 1 hit PS00763, GLUTATHIONE_PEROXID_2, 1 hit PS51355, GLUTATHIONE_PEROXID_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GPX1_HUMAN | |
Accessioni | P07203Primary (citable) accession number: P07203 Secondary accession number(s): E9PAS1, Q7Z5H1, Q9BW12 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1988 |
Last sequence update: | November 2, 2010 | |
Last modified: | February 10, 2021 | |
This is version 225 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 3
Human chromosome 3: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families