UniProtKB - P07197 (NFM_HUMAN)
Neurofilament medium polypeptide
NEFM
Functioni
Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).
By similarityGO - Molecular functioni
- microtubule binding Source: BHF-UCL
- structural constituent of cytoskeleton Source: ProtInc
GO - Biological processi
- neurofilament bundle assembly Source: BHF-UCL
Enzyme and pathway databases
PathwayCommonsi | P07197 |
SignaLinki | P07197 |
SIGNORi | P07197 |
Names & Taxonomyi
Protein namesi | Recommended name: Neurofilament medium polypeptideShort name: NF-M Alternative name(s): 160 kDa neurofilament protein Neurofilament 3 Neurofilament triplet M protein |
Gene namesi | Name:NEFM Synonyms:NEF3, NFM |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:7734, NEFM |
MIMi | 162250, gene |
neXtProti | NX_P07197 |
VEuPathDBi | HostDB:ENSG00000104722 |
Subcellular locationi
Cytoskeleton
- cytoskeleton By similarity
Other locations
- axon By similarity
Cytoskeleton
- intermediate filament cytoskeleton Source: HPA
- neurofilament Source: ProtInc
Other locations
- axon Source: UniProtKB
- neurofibrillary tangle Source: BHF-UCL
Keywords - Cellular componenti
Cell projection, Cytoplasm, Cytoskeleton, Intermediate filamentPathology & Biotechi
Organism-specific databases
DisGeNETi | 4741 |
OpenTargetsi | ENSG00000104722 |
PharmGKBi | PA162397442 |
Miscellaneous databases
Pharosi | P07197, Tbio |
Genetic variation databases
BioMutai | NEFM |
DMDMi | 281185500 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000063794 | 2 – 916 | Neurofilament medium polypeptideAdd BLAST | 915 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineBy similarity | 1 | |
Modified residuei | 30 | PhosphoserineBy similarity | 1 | |
Modified residuei | 42 | Omega-N-methylarginineBy similarity | 1 | |
Glycosylationi | 47 | O-linked (GlcNAc) threonineBy similarity | 1 | |
Modified residuei | 99 | PhosphoserineBy similarity | 1 | |
Modified residuei | 226 | PhosphoserineBy similarity | 1 | |
Modified residuei | 320 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 346 | PhosphoserineBy similarity | 1 | |
Modified residuei | 418 | PhosphoserineBy similarity | 1 | |
Glycosylationi | 431 | O-linked (GlcNAc) threonineBy similarity | 1 | |
Modified residuei | 467 | PhosphoserineBy similarity | 1 | |
Modified residuei | 483 | PhosphoserineBy similarity | 1 | |
Modified residuei | 511 | PhosphoserineBy similarity | 1 | |
Modified residuei | 545 | PhosphoserineBy similarity | 1 | |
Modified residuei | 553 | PhosphoserineBy similarity | 1 | |
Modified residuei | 558 | PhosphoserineBy similarity | 1 | |
Modified residuei | 559 | PhosphoserineBy similarity | 1 | |
Modified residuei | 571 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 641 | PhosphoserineBy similarity | 1 | |
Modified residuei | 646 | PhosphoserineBy similarity | 1 | |
Modified residuei | 680 | PhosphoserineBy similarity | 1 | |
Modified residuei | 685 | PhosphoserineBy similarity | 1 | |
Modified residuei | 736 | PhosphoserineBy similarity | 1 | |
Modified residuei | 783 | PhosphoserineBy similarity | 1 | |
Modified residuei | 821 | PhosphoserineBy similarity | 1 | |
Modified residuei | 837 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Glycoprotein, Methylation, PhosphoproteinProteomic databases
EPDi | P07197 |
jPOSTi | P07197 |
MassIVEi | P07197 |
MaxQBi | P07197 |
PaxDbi | P07197 |
PeptideAtlasi | P07197 |
PRIDEi | P07197 |
ProteomicsDBi | 19213 51962 [P07197-1] |
2D gel databases
UCD-2DPAGEi | P07197 |
PTM databases
GlyGeni | P07197, 7 sites, 1 O-linked glycan (6 sites) |
iPTMneti | P07197 |
PhosphoSitePlusi | P07197 |
SwissPalmi | P07197 |
Expressioni
Gene expression databases
Bgeei | ENSG00000104722, Expressed in dorsal root ganglion and 178 other tissues |
ExpressionAtlasi | P07197, baseline and differential |
Genevisiblei | P07197, HS |
Organism-specific databases
HPAi | ENSG00000104722, Tissue enriched (brain) |
Interactioni
Subunit structurei
Forms heterodimers with NEFL; which can further hetero-oligomerize (in vitro) (By similarity). Forms heterodimers with INA (in vitro) (By similarity).
By similarityBinary interactionsi
P07197
With | #Exp. | IntAct |
---|---|---|
JUN [P05412] | 2 | EBI-1105035,EBI-852823 |
NEFL [P07196] | 4 | EBI-1105035,EBI-475646 |
RB1 [P06400] | 2 | EBI-1105035,EBI-491274 |
SMAD2 [Q15796] | 3 | EBI-1105035,EBI-1040141 |
VIM [P08670] | 5 | EBI-1105035,EBI-353844 |
GO - Molecular functioni
- microtubule binding Source: BHF-UCL
Protein-protein interaction databases
BioGRIDi | 110818, 111 interactors |
IntActi | P07197, 47 interactors |
MINTi | P07197 |
STRINGi | 9606.ENSP00000221166 |
Miscellaneous databases
RNActi | P07197, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 101 – 412 | IF rodPROSITE-ProRule annotationAdd BLAST | 312 | |
Repeati | 614 – 626 | 1Add BLAST | 13 | |
Repeati | 627 – 639 | 2Add BLAST | 13 | |
Repeati | 640 – 652 | 3Add BLAST | 13 | |
Repeati | 653 – 665 | 4Add BLAST | 13 | |
Repeati | 666 – 678 | 5Add BLAST | 13 | |
Repeati | 679 – 691 | 6Add BLAST | 13 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 51 | DisorderedSequence analysisAdd BLAST | 51 | |
Regioni | 2 – 104 | HeadAdd BLAST | 103 | |
Regioni | 105 – 136 | Coil 1AAdd BLAST | 32 | |
Regioni | 137 – 149 | Linker 1Add BLAST | 13 | |
Regioni | 150 – 248 | Coil 1BAdd BLAST | 99 | |
Regioni | 249 – 265 | Linker 12Add BLAST | 17 | |
Regioni | 266 – 287 | Coil 2AAdd BLAST | 22 | |
Regioni | 288 – 291 | Linker 2 | 4 | |
Regioni | 292 – 412 | Coil 2BAdd BLAST | 121 | |
Regioni | 413 – 916 | TailAdd BLAST | 504 | |
Regioni | 485 – 851 | DisorderedSequence analysisAdd BLAST | 367 | |
Regioni | 614 – 691 | 6 X 13 AA approximate tandem repeats of K-S-P-V-[PS]-K-S-P-V-E-E-[KA]-[GAK]Add BLAST | 78 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 504 – 522 | Basic and acidic residuesSequence analysisAdd BLAST | 19 | |
Compositional biasi | 523 – 545 | Acidic residuesSequence analysisAdd BLAST | 23 | |
Compositional biasi | 546 – 562 | Basic and acidic residuesSequence analysisAdd BLAST | 17 | |
Compositional biasi | 563 – 579 | Acidic residuesSequence analysisAdd BLAST | 17 | |
Compositional biasi | 580 – 740 | Basic and acidic residuesSequence analysisAdd BLAST | 161 | |
Compositional biasi | 752 – 830 | Basic and acidic residuesSequence analysisAdd BLAST | 79 |
Sequence similaritiesi
Keywords - Domaini
Coiled coil, RepeatPhylogenomic databases
eggNOGi | KOG1216, Eukaryota |
GeneTreei | ENSGT00940000154418 |
HOGENOMi | CLU_524743_0_0_1 |
InParanoidi | P07197 |
OMAi | GPIFTHR |
OrthoDBi | 898483at2759 |
PhylomeDBi | P07197 |
TreeFami | TF330122 |
Family and domain databases
InterProi | View protein in InterPro IPR018039, IF_conserved IPR039008, IF_rod_dom IPR006821, Intermed_filament_DNA-bd IPR002957, Keratin_I IPR027697, NF-M |
PANTHERi | PTHR45652:SF3, PTHR45652:SF3, 1 hit |
Pfami | View protein in Pfam PF00038, Filament, 1 hit PF04732, Filament_head, 1 hit |
PRINTSi | PR01248, TYPE1KERATIN |
SMARTi | View protein in SMART SM01391, Filament, 1 hit |
PROSITEi | View protein in PROSITE PS00226, IF_ROD_1, 1 hit PS51842, IF_ROD_2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSYTLDSLGN PSAYRRVTET RSSFSRVSGS PSSGFRSQSW SRGSPSTVSS
60 70 80 90 100
SYKRSMLAPR LAYSSAMLSS AESSLDFSQS SSLLNGGSGP GGDYKLSRSN
110 120 130 140 150
EKEQLQGLND RFAGYIEKVH YLEQQNKEIE AEIQALRQKQ ASHAQLGDAY
160 170 180 190 200
DQEIRELRAT LEMVNHEKAQ VQLDSDHLEE DIHRLKERFE EEARLRDDTE
210 220 230 240 250
AAIRALRKDI EEASLVKVEL DKKVQSLQDE VAFLRSNHEE EVADLLAQIQ
260 270 280 290 300
ASHITVERKD YLKTDISTAL KEIRSQLESH SDQNMHQAEE WFKCRYAKLT
310 320 330 340 350
EAAEQNKEAI RSAKEEIAEY RRQLQSKSIE LESVRGTKES LERQLSDIEE
360 370 380 390 400
RHNHDLSSYQ DTIQQLENEL RGTKWEMARH LREYQDLLNV KMALDIEIAA
410 420 430 440 450
YRKLLEGEET RFSTFAGSIT GPLYTHRPPI TISSKIQKPK VEAPKLKVQH
460 470 480 490 500
KFVEEIIEET KVEDEKSEME EALTAITEEL AVSMKEEKKE AAEEKEEEPE
510 520 530 540 550
AEEEEVAAKK SPVKATAPEV KEEEGEKEEE EGQEEEEEED EGAKSDQAEE
560 570 580 590 600
GGSEKEGSSE KEEGEQEEGE TEAEAEGEEA EAKEEKKVEE KSEEVATKEE
610 620 630 640 650
LVADAKVEKP EKAKSPVPKS PVEEKGKSPV PKSPVEEKGK SPVPKSPVEE
660 670 680 690 700
KGKSPVPKSP VEEKGKSPVS KSPVEEKAKS PVPKSPVEEA KSKAEVGKGE
710 720 730 740 750
QKEEEEKEVK EAPKEEKVEK KEEKPKDVPE KKKAESPVKE EAVAEVVTIT
760 770 780 790 800
KSVKVHLEKE TKEEGKPLQQ EKEKEKAGGE GGSEEEGSDK GAKGSRKEDI
810 820 830 840 850
AVNGEVEGKE EVEQETKEKG SGREEEKGVV TNGLDLSPAD EKKGGDKSEE
860 870 880 890 900
KVVVTKTVEK ITSEGGDGAT KYITKSVTVT QKVEEHEETF EEKLVSTKKV
910
EKVTSHAIVK EVTQSD
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE7EMV2 | E7EMV2_HUMAN | 160 kDa neurofilament protein | NEFM | 698 | Annotation score: | ||
E7ESP9 | E7ESP9_HUMAN | 160 kDa neurofilament protein | NEFM | 877 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 482 | V → A in CAA68276 (PubMed:3608989).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_060732 | 439 | P → TCombined sources5 PublicationsCorresponds to variant dbSNP:rs196864Ensembl. | 1 | |
Natural variantiVAR_056024 | 725 | P → Q. Corresponds to variant dbSNP:rs196863EnsemblClinVar. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_046306 | 1 – 376 | Missing in isoform 2. 1 PublicationAdd BLAST | 376 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y00067 Genomic DNA Translation: CAA68276.1 EF560736 mRNA Translation: ABQ59046.1 AK294681 mRNA Translation: BAG57843.1 AF106564 Genomic DNA No translation available. CH471080 Genomic DNA Translation: EAW63602.1 BC096757 mRNA Translation: AAH96757.1 |
CCDSi | CCDS47831.1 [P07197-2] CCDS6046.1 [P07197-1] |
PIRi | A27864 |
RefSeqi | NP_005373.2, NM_005382.2 [P07197-1] |
Genome annotation databases
Ensembli | ENST00000221166; ENSP00000221166; ENSG00000104722 ENST00000433454; ENSP00000412295; ENSG00000104722 [P07197-2] |
GeneIDi | 4741 |
KEGGi | hsa:4741 |
MANE-Selecti | ENST00000221166.10; ENSP00000221166.5; NM_005382.2; NP_005373.2 |
UCSCi | uc003xed.5, human [P07197-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Web resourcesi
Human Intermediate Filament Mutation Database |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y00067 Genomic DNA Translation: CAA68276.1 EF560736 mRNA Translation: ABQ59046.1 AK294681 mRNA Translation: BAG57843.1 AF106564 Genomic DNA No translation available. CH471080 Genomic DNA Translation: EAW63602.1 BC096757 mRNA Translation: AAH96757.1 |
CCDSi | CCDS47831.1 [P07197-2] CCDS6046.1 [P07197-1] |
PIRi | A27864 |
RefSeqi | NP_005373.2, NM_005382.2 [P07197-1] |
3D structure databases
SMRi | P07197 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 110818, 111 interactors |
IntActi | P07197, 47 interactors |
MINTi | P07197 |
STRINGi | 9606.ENSP00000221166 |
PTM databases
GlyGeni | P07197, 7 sites, 1 O-linked glycan (6 sites) |
iPTMneti | P07197 |
PhosphoSitePlusi | P07197 |
SwissPalmi | P07197 |
Genetic variation databases
BioMutai | NEFM |
DMDMi | 281185500 |
2D gel databases
UCD-2DPAGEi | P07197 |
Proteomic databases
EPDi | P07197 |
jPOSTi | P07197 |
MassIVEi | P07197 |
MaxQBi | P07197 |
PaxDbi | P07197 |
PeptideAtlasi | P07197 |
PRIDEi | P07197 |
ProteomicsDBi | 19213 51962 [P07197-1] |
Protocols and materials databases
Antibodypediai | 4396, 702 antibodies from 47 providers |
DNASUi | 4741 |
Genome annotation databases
Ensembli | ENST00000221166; ENSP00000221166; ENSG00000104722 ENST00000433454; ENSP00000412295; ENSG00000104722 [P07197-2] |
GeneIDi | 4741 |
KEGGi | hsa:4741 |
MANE-Selecti | ENST00000221166.10; ENSP00000221166.5; NM_005382.2; NP_005373.2 |
UCSCi | uc003xed.5, human [P07197-1] |
Organism-specific databases
CTDi | 4741 |
DisGeNETi | 4741 |
GeneCardsi | NEFM |
HGNCi | HGNC:7734, NEFM |
HPAi | ENSG00000104722, Tissue enriched (brain) |
MIMi | 162250, gene |
neXtProti | NX_P07197 |
OpenTargetsi | ENSG00000104722 |
PharmGKBi | PA162397442 |
VEuPathDBi | HostDB:ENSG00000104722 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1216, Eukaryota |
GeneTreei | ENSGT00940000154418 |
HOGENOMi | CLU_524743_0_0_1 |
InParanoidi | P07197 |
OMAi | GPIFTHR |
OrthoDBi | 898483at2759 |
PhylomeDBi | P07197 |
TreeFami | TF330122 |
Enzyme and pathway databases
PathwayCommonsi | P07197 |
SignaLinki | P07197 |
SIGNORi | P07197 |
Miscellaneous databases
BioGRID-ORCSi | 4741, 27 hits in 1033 CRISPR screens |
ChiTaRSi | NEFM, human |
GeneWikii | NEFM |
GenomeRNAii | 4741 |
Pharosi | P07197, Tbio |
PROi | PR:P07197 |
RNActi | P07197, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000104722, Expressed in dorsal root ganglion and 178 other tissues |
ExpressionAtlasi | P07197, baseline and differential |
Genevisiblei | P07197, HS |
Family and domain databases
InterProi | View protein in InterPro IPR018039, IF_conserved IPR039008, IF_rod_dom IPR006821, Intermed_filament_DNA-bd IPR002957, Keratin_I IPR027697, NF-M |
PANTHERi | PTHR45652:SF3, PTHR45652:SF3, 1 hit |
Pfami | View protein in Pfam PF00038, Filament, 1 hit PF04732, Filament_head, 1 hit |
PRINTSi | PR01248, TYPE1KERATIN |
SMARTi | View protein in SMART SM01391, Filament, 1 hit |
PROSITEi | View protein in PROSITE PS00226, IF_ROD_1, 1 hit PS51842, IF_ROD_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NFM_HUMAN | |
Accessioni | P07197Primary (citable) accession number: P07197 Secondary accession number(s): B4DGN2, E9PBF7, Q4QRK6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1988 |
Last sequence update: | December 15, 2009 | |
Last modified: | February 23, 2022 | |
This is version 210 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 8
Human chromosome 8: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families