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Protein

Aequorin-1

Gene
N/A
Organism
Aequorea victoria (Jellyfish)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Ca2+-dependent bioluminescence photoprotein. Displays an emission peak at 470 nm (blue light). Trace amounts of calcium ion trigger the intramolecular oxidation of the chromophore, coelenterazine into coelenteramide and CO2 with the concomitant emission of light.

Caution

Was originally thought to have a internal disulfide bond.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi31 – 421Add BLAST12
Calcium bindingi124 – 1352Add BLAST12
Calcium bindingi160 – 1713Add BLAST12

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionPhotoprotein
Biological processLuminescence
LigandCalcium, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-20288
BRENDAi1.13.12.5 8923

Names & Taxonomyi

Protein namesi
Recommended name:
Aequorin-1
OrganismiAequorea victoria (Jellyfish)
Taxonomic identifieri6100 [NCBI]
Taxonomic lineageiEukaryotaMetazoaCnidariaHydrozoaHydroidolinaLeptothecataAequoreidaeAequorea

Pathology & Biotechi

Biotechnological usei

Aequorin is used as an intracellular Ca2+ indicator. Aequorin has a number of advantages over other Ca2+ indicators, for example, low leakage rate from cells, lack of intracellular compartmentalization or sequestration and it does not disrupt cell functions or embryo development.

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi196Missing : Loss of bioluminescence. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00000041261 – 71 Publication7
ChainiPRO_00000041278 – 196Aequorin-1Add BLAST189

Post-translational modificationi

The reduction of the disulfide bond is necessary to regenerate aequorin from apoaequorin.

Keywords - PTMi

Disulfide bond

Structurei

Secondary structure

1196
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP07164
SMRiP07164
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07164

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini18 – 53EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini54 – 108EF-hand 2PROSITE-ProRule annotationAdd BLAST55
Domaini111 – 146EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini147 – 182EF-hand 4PROSITE-ProRule annotationAdd BLAST36

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni47 – 57May interact with the chromophoreAdd BLAST11
Regioni62 – 72May interact with the chromophoreAdd BLAST11
Regioni107 – 117May interact with the chromophoreAdd BLAST11

Sequence similaritiesi

Belongs to the aequorin family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

KOiK22619

Family and domain databases

CDDicd00051 EFh, 1 hit
InterProiView protein in InterPro
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
PfamiView protein in Pfam
PF13202 EF-hand_5, 2 hits
SMARTiView protein in SMART
SM00054 EFh, 3 hits
SUPFAMiSSF47473 SSF47473, 1 hit
PROSITEiView protein in PROSITE
PS00018 EF_HAND_1, 3 hits
PS50222 EF_HAND_2, 3 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07164-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTSEQYSVKL TPDFDNPKWI GRHKHMFNFL DVNHNGRISL DEMVYKASDI
60 70 80 90 100
VINNLGATPE QAKRHKDAVE AFFGGAGMKY GVETEWPEYI EGWKRLASEE
110 120 130 140 150
LKRYSKNQIT LIRLWGDALF DIIDKDQNGA ISLDEWKAYT KSDGIIQSSE
160 170 180 190
DCEETFRVCD IDESGQLDVD EMTRQHLGFW YTMDPACEKL YGGAVP
Length:196
Mass (Da):22,514
Last modified:April 1, 1988 - v1
Checksum:i9AA5B636288A5B8F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16103 mRNA Translation: AAA27716.1
PIRiA26623

Genome annotation databases

KEGGiag:AAA27716

Similar proteinsi

Cross-referencesi

Web resourcesi

Wikipedia

Aequorin entry

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16103 mRNA Translation: AAA27716.1
PIRiA26623

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SL8X-ray1.70A7-196[»]
ProteinModelPortaliP07164
SMRiP07164
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:AAA27716

Phylogenomic databases

KOiK22619

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-20288
BRENDAi1.13.12.5 8923

Miscellaneous databases

EvolutionaryTraceiP07164

Family and domain databases

CDDicd00051 EFh, 1 hit
InterProiView protein in InterPro
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
PfamiView protein in Pfam
PF13202 EF-hand_5, 2 hits
SMARTiView protein in SMART
SM00054 EFh, 3 hits
SUPFAMiSSF47473 SSF47473, 1 hit
PROSITEiView protein in PROSITE
PS00018 EF_HAND_1, 3 hits
PS50222 EF_HAND_2, 3 hits
ProtoNetiSearch...

Entry informationi

Entry nameiAEQ1_AEQVI
AccessioniPrimary (citable) accession number: P07164
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: June 20, 2018
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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