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Protein

Stromelysin-2

Gene

Mmp10

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Can degrade fibronectin, gelatins of type I, III, IV, and V; weakly collagens III, IV, and V. Activates procollagenase.

Catalytic activityi

Similar to stromelysin 1, but action on collagen types III, IV and V is weak.

Cofactori

Protein has several cofactor binding sites:
  • Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity
  • Ca2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi92Zinc; in inhibited formBy similarity1
Metal bindingi168Zinc 1By similarity1
Metal bindingi170Zinc 1By similarity1
Metal bindingi183Zinc 1By similarity1
Metal bindingi196Zinc 1By similarity1
Metal bindingi218Zinc 2; catalyticBy similarity1
Active sitei219PROSITE-ProRule annotation1
Metal bindingi222Zinc 2; catalyticBy similarity1
Metal bindingi228Zinc 2; catalyticBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Metalloprotease, Protease
Biological processCollagen degradation
LigandCalcium, Metal-binding, Zinc

Protein family/group databases

MEROPSiM10.011

Names & Taxonomyi

Protein namesi
Recommended name:
Stromelysin-2 (EC:3.4.24.22)
Short name:
SL-2
Alternative name(s):
Matrix metalloproteinase-10
Short name:
MMP-10
Transformation-associated protein 34A
Transin-2
Gene namesi
Name:Mmp10
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620192 Mmp10

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 17CuratedAdd BLAST17
PropeptideiPRO_000002876818 – 99Activation peptideBy similarityAdd BLAST82
ChainiPRO_0000028769100 – 476Stromelysin-2Add BLAST377

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi289 ↔ 476By similarity

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

PaxDbiP07152
PRIDEiP07152

Interactioni

Protein-protein interaction databases

IntActiP07152, 1 interactor
STRINGi10116.ENSRNOP00000013118

Structurei

3D structure databases

ProteinModelPortaliP07152
SMRiP07152
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati286 – 335Hemopexin 1Add BLAST50
Repeati336 – 382Hemopexin 2Add BLAST47
Repeati384 – 432Hemopexin 3Add BLAST49
Repeati433 – 476Hemopexin 4Add BLAST44

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi90 – 97Cysteine switchBy similarity8

Domaini

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Sequence similaritiesi

Belongs to the peptidase M10A family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG1565 Eukaryota
ENOG410XQ5D LUCA
HOGENOMiHOG000217927
HOVERGENiHBG052484
InParanoidiP07152
KOiK01396
PhylomeDBiP07152

Family and domain databases

CDDicd00094 HX, 1 hit
cd04278 ZnMc_MMP, 1 hit
Gene3Di1.10.101.10, 1 hit
2.110.10.10, 1 hit
3.40.390.10, 2 hits
InterProiView protein in InterPro
IPR000585 Hemopexin-like_dom
IPR036375 Hemopexin-like_dom_sf
IPR018487 Hemopexin-like_repeat
IPR018486 Hemopexin_CS
IPR033739 M10A_MMP
IPR024079 MetalloPept_cat_dom_sf
IPR001818 Pept_M10_metallopeptidase
IPR021190 Pept_M10A
IPR021158 Pept_M10A_Zn_BS
IPR006026 Peptidase_Metallo
IPR002477 Peptidoglycan-bd-like
IPR036365 PGBD-like_sf
IPR036366 PGBDSf
PfamiView protein in Pfam
PF00045 Hemopexin, 4 hits
PF00413 Peptidase_M10, 1 hit
PF01471 PG_binding_1, 1 hit
PIRSFiPIRSF001191 Peptidase_M10A_matrix, 1 hit
PRINTSiPR00138 MATRIXIN
SMARTiView protein in SMART
SM00120 HX, 4 hits
SM00235 ZnMc, 1 hit
SUPFAMiSSF47090 SSF47090, 1 hit
SSF50923 SSF50923, 1 hit
PROSITEiView protein in PROSITE
PS00546 CYSTEINE_SWITCH, 1 hit
PS00024 HEMOPEXIN, 1 hit
PS51642 HEMOPEXIN_2, 4 hits
PS00142 ZINC_PROTEASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07152-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPLAILVLL CFPICSAYPL HGAVRQDHST MDLAQQYLEK YYNFRKNEKQ
60 70 80 90 100
FFKRKDSSPV VKKIEEMQKF LGLEMTGKLD SNTVEMMHKP RCGVPDVGGF
110 120 130 140 150
STFPGSPKWR KNHISYRIVN YTLDLPRESV DSAIERALKV WEEVTPLTFS
160 170 180 190 200
RISEGEADIM ISFAVGEHGD FYPFDGVGQS LAHAYPPGPG FYGDAHFDDD
210 220 230 240 250
EKWSLGPSGT NLFLVAAHEL GHSLGLFHSN NKESLMYPVY RFSTSQANIR
260 270 280 290 300
LSQDDIEGIQ SLYGARPSSD ATVVPVPSVS PKPETPVKCD PALSFDAVTM
310 320 330 340 350
LRGEFLFFKD RHFWRRTQWN PEPEFHLISA FWPSLPSGLD AAYEANNKDR
360 370 380 390 400
VLIFKGSQFW AVRGNEVQAG YPKRIHTLGF PPTVKKIDAA VFEKEKKKTY
410 420 430 440 450
FFVGDKYWRF DETRQLMDKG FPRLITDDFP GIEPQVDAVL HAFGFFYFFC
460 470
GSSQFEFDPN ARTVTHTLKS NSWLLC
Length:476
Mass (Da):54,222
Last modified:April 1, 1988 - v1
Checksum:iB556B6FB1D8BA7EE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05083 mRNA Translation: CAA28739.1
M65253 mRNA Translation: AAA42202.1
PIRiB26403 KCRTS2
RefSeqiNP_598198.1, NM_133514.1
UniGeneiRn.9946

Genome annotation databases

GeneIDi117061
KEGGirno:117061
UCSCiRGD:620192 rat

Similar proteinsi

Entry informationi

Entry nameiMMP10_RAT
AccessioniPrimary (citable) accession number: P07152
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: June 20, 2018
This is version 154 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

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