UniProtKB - P07147 (TYRP1_MOUSE)
Protein
5,6-dihydroxyindole-2-carboxylic acid oxidase
Gene
Tyrp1
Organism
Mus musculus (Mouse)
Status
Functioni
Plays a role in melanin biosynthesis (PubMed:2245916). Catalyzes the oxidation of 5,6-dihydroxyindole-2-carboxylic acid (DHICA) into indole-5,6-quinone-2-carboxylic acid (PubMed:7813420). May regulate or influence the type of melanin synthesized (PubMed:7813420, PubMed:2245916). Also to a lower extent, capable of hydroxylating tyrosine and producing melanin (PubMed:1537333).3 Publications
Caution
The precise function of this protein in melanin biosynthesis is still under debate. DHICA oxidase activity is controversial. The mouse protein has been shown to have DHICA oxidase activity (PubMed:7813420). In contrast, the human protein was shown lack DHICA oxidase activity, or to have DHICA oxidase activity only in the presence of Cu2+, but not with Zn2+ (By similarity).By similarityCurated1 Publication
Cofactori
Cu2+By similarity, Zn2+By similarityNote: Contains bound zinc ions after heterologous expression in insect cells.By similarity
: melanin biosynthesis Pathwayi
This protein is involved in the pathway melanin biosynthesis, which is part of Pigment biosynthesis.1 PublicationView all proteins of this organism that are known to be involved in the pathway melanin biosynthesis and in Pigment biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 192 | Zinc ABy similarity | 1 | |
Metal bindingi | 215 | Zinc ABy similarity | 1 | |
Metal bindingi | 224 | Zinc ABy similarity | 1 | |
Metal bindingi | 377 | Zinc BBy similarity | 1 | |
Metal bindingi | 381 | Zinc BBy similarity | 1 | |
Metal bindingi | 404 | Zinc BBy similarity | 1 |
GO - Molecular functioni
- identical protein binding Source: MGI
- metal ion binding Source: UniProtKB-KW
- monophenol monooxygenase activity Source: UniProtKB
- protein homodimerization activity Source: UniProtKB
GO - Biological processi
- acetoacetic acid metabolic process Source: MGI
- melanin biosynthetic process from tyrosine Source: UniProtKB
- melanin metabolic process Source: MGI
- melanocyte differentiation Source: MGI
- melanosome organization Source: MGI
- pigmentation Source: MGI
- positive regulation of melanin biosynthetic process Source: CACAO
Keywordsi
Molecular function | Monooxygenase, Oxidoreductase |
Biological process | Melanin biosynthesis |
Ligand | Copper, Metal-binding, Zinc |
Enzyme and pathway databases
BioCyci | MetaCyc:MONOMER-15620 |
Reactomei | R-MMU-5662702, Melanin biosynthesis |
UniPathwayi | UPA00785 |
Names & Taxonomyi
Protein namesi | Recommended name: 5,6-dihydroxyindole-2-carboxylic acid oxidase (EC:1.14.18.-1 Publication)Short name: DHICA oxidase1 Publication Alternative name(s): Brown locus protein Catalase B Tyrosinase-related protein 1 Short name: TRP Short name: TRP-1 Short name: TRP1 |
Gene namesi | Name:Tyrp1 Synonyms:Tyrp-1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:98881, Tyrp1 |
Subcellular locationi
Other locations
- Melanosome membrane 2 Publications; Single-pass type I membrane protein 1 Publication
- Melanosome 1 Publication
Note: Located to mature stage III and IV melanosomes and apposed endosomal tubular membranes. Transported to pigmented melanosomes by the BLOC-1 complex. Proper trafficking to melanosome is regulated by SGSM2, ANKRD27, RAB9A, RAB32 and RAB38.2 Publications
Endosome
- endosome membrane Source: MGI
Other locations
- clathrin-coated endocytic vesicle membrane Source: MGI
- cytoplasm Source: MGI
- integral component of membrane Source: UniProtKB-KW
- intracellular vesicle Source: MGI
- melanosome Source: UniProtKB
- melanosome membrane Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 25 – 477 | Lumenal, melanosomeSequence analysisAdd BLAST | 453 | |
Transmembranei | 478 – 501 | HelicalSequence analysisAdd BLAST | 24 | |
Topological domaini | 502 – 537 | CytoplasmicSequence analysisAdd BLAST | 36 |
Keywords - Cellular componenti
MembranePathology & Biotechi
Involvement in diseasei
Defects in Tyrp1 are the cause of the brown (b) phenotype. Brown mice have a brown or hypopigmented coat.1 Publication
Keywords - Diseasei
Albinism, Disease variantChemistry databases
ChEMBLi | CHEMBL3638355 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 24 | Add BLAST | 24 | |
ChainiPRO_0000035890 | 25 – 537 | 5,6-dihydroxyindole-2-carboxylic acid oxidaseAdd BLAST | 513 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 30 ↔ 41 | By similarity | ||
Disulfide bondi | 42 ↔ 65 | By similarity | ||
Disulfide bondi | 56 ↔ 99 | By similarity | ||
Glycosylationi | 96 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 101 ↔ 110 | By similarity | ||
Glycosylationi | 104 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 113 ↔ 122 | By similarity | ||
Glycosylationi | 181 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 258 ↔ 261 | By similarity | ||
Disulfide bondi | 290 ↔ 303 | By similarity | ||
Glycosylationi | 304 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 350 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 385 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Post-translational modificationi
Glycosylated.1 Publication
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
PaxDbi | P07147 |
PRIDEi | P07147 |
ProteomicsDBi | 297764 |
PTM databases
GlyGeni | P07147, 6 sites |
iPTMneti | P07147 |
PhosphoSitePlusi | P07147 |
Expressioni
Tissue specificityi
Pigment cells.
Gene expression databases
Bgeei | ENSMUSG00000005994, Expressed in iris and 92 other tissues |
ExpressionAtlasi | P07147, baseline and differential |
Genevisiblei | P07147, MM |
Interactioni
Subunit structurei
Binary interactionsi
P07147
With | #Exp. | IntAct |
---|---|---|
Tyr [P11344] | 8 | EBI-821614,EBI-821603 |
GO - Molecular functioni
- identical protein binding Source: MGI
- protein homodimerization activity Source: UniProtKB
Protein-protein interaction databases
IntActi | P07147, 1 interactor |
STRINGi | 10090.ENSMUSP00000006151 |
Chemistry databases
BindingDBi | P07147 |
Miscellaneous databases
RNActi | P07147, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the tyrosinase family.Curated
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502QRNA, Eukaryota |
GeneTreei | ENSGT00940000155804 |
HOGENOMi | CLU_038693_1_0_1 |
InParanoidi | P07147 |
OMAi | RPHSHHY |
OrthoDBi | 518234at2759 |
PhylomeDBi | P07147 |
TreeFami | TF315865 |
Family and domain databases
Gene3Di | 1.10.1280.10, 1 hit |
InterProi | View protein in InterPro IPR002227, Tyrosinase_Cu-bd IPR008922, Unchr_di-copper_centre |
Pfami | View protein in Pfam PF00264, Tyrosinase, 1 hit |
PRINTSi | PR00092, TYROSINASE |
SUPFAMi | SSF48056, SSF48056, 1 hit |
PROSITEi | View protein in PROSITE PS00497, TYROSINASE_1, 1 hit PS00498, TYROSINASE_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
P07147-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKSYNVLPLA YISLFLMLFY QVWAQFPREC ANIEALRRGV CCPDLLPSSG
60 70 80 90 100
PGTDPCGSSS GRGRCVAVIA DSRPHSRHYP HDGKDDREAW PLRFFNRTCQ
110 120 130 140 150
CNDNFSGHNC GTCRPGWRGA ACNQKILTVR RNLLDLSPEE KSHFVRALDM
160 170 180 190 200
AKRTTHPQFV IATRRLEDIL GPDGNTPQFE NISVYNYFVW THYYSVKKTF
210 220 230 240 250
LGTGQESFGD VDFSHEGPAF LTWHRYHLLQ LERDMQEMLQ EPSFSLPYWN
260 270 280 290 300
FATGKNVCDV CTDDLMGSRS NFDSTLISPN SVFSQWRVVC ESLEEYDTLG
310 320 330 340 350
TLCNSTEGGP IRRNPAGNVG RPAVQRLPEP QDVTQCLEVR VFDTPPFYSN
360 370 380 390 400
STDSFRNTVE GYSAPTGKYD PAVRSLHNLA HLFLNGTGGQ THLSPNDPIF
410 420 430 440 450
VLLHTFTDAV FDEWLRRYNA DISTFPLENA PIGHNRQYNM VPFWPPVTNT
460 470 480 490 500
EMFVTAPDNL GYAYEVQWPG QEFTVSEIIT IAVVAALLLV AAIFGVASCL
510 520 530
IRSRSTKNEA NQPLLTDHYQ RYAEDYEELP NPNHSMV
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA2ADJ4 | A2ADJ4_MOUSE | 5,6-dihydroxyindole-2-carboxylic ac... | Tyrp1 RP23-372M15.1-003 | 229 | Annotation score: | ||
A2ADJ6 | A2ADJ6_MOUSE | 5,6-dihydroxyindole-2-carboxylic ac... | Tyrp1 RP23-372M15.1-002 | 176 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 110 | C → Y in brown. 1 Publication | 1 | |
Natural varianti | 326 | R → H in brown. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X03687 mRNA Translation: CAA27323.1 BC076598 mRNA Translation: AAH76598.1 |
CCDSi | CCDS18290.1 |
PIRi | A24933, YRMSB6 |
RefSeqi | NP_001268943.1, NM_001282014.1 NP_001268944.1, NM_001282015.1 NP_112479.1, NM_031202.3 |
Genome annotation databases
Ensembli | ENSMUST00000006151; ENSMUSP00000006151; ENSMUSG00000005994 ENSMUST00000102831; ENSMUSP00000099895; ENSMUSG00000005994 |
GeneIDi | 22178 |
KEGGi | mmu:22178 |
UCSCi | uc008tjq.3, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X03687 mRNA Translation: CAA27323.1 BC076598 mRNA Translation: AAH76598.1 |
CCDSi | CCDS18290.1 |
PIRi | A24933, YRMSB6 |
RefSeqi | NP_001268943.1, NM_001282014.1 NP_001268944.1, NM_001282015.1 NP_112479.1, NM_031202.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6MP0 | X-ray | 2.00 | A | 455-463 | [»] | |
6MP1 | X-ray | 2.21 | A | 455-463 | [»] | |
SMRi | P07147 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | P07147, 1 interactor |
STRINGi | 10090.ENSMUSP00000006151 |
Chemistry databases
BindingDBi | P07147 |
ChEMBLi | CHEMBL3638355 |
PTM databases
GlyGeni | P07147, 6 sites |
iPTMneti | P07147 |
PhosphoSitePlusi | P07147 |
Proteomic databases
PaxDbi | P07147 |
PRIDEi | P07147 |
ProteomicsDBi | 297764 |
Protocols and materials databases
Antibodypediai | 745, 740 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000006151; ENSMUSP00000006151; ENSMUSG00000005994 ENSMUST00000102831; ENSMUSP00000099895; ENSMUSG00000005994 |
GeneIDi | 22178 |
KEGGi | mmu:22178 |
UCSCi | uc008tjq.3, mouse |
Organism-specific databases
CTDi | 7306 |
MGIi | MGI:98881, Tyrp1 |
Phylogenomic databases
eggNOGi | ENOG502QRNA, Eukaryota |
GeneTreei | ENSGT00940000155804 |
HOGENOMi | CLU_038693_1_0_1 |
InParanoidi | P07147 |
OMAi | RPHSHHY |
OrthoDBi | 518234at2759 |
PhylomeDBi | P07147 |
TreeFami | TF315865 |
Enzyme and pathway databases
UniPathwayi | UPA00785 |
BioCyci | MetaCyc:MONOMER-15620 |
Reactomei | R-MMU-5662702, Melanin biosynthesis |
Miscellaneous databases
BioGRID-ORCSi | 22178, 2 hits in 53 CRISPR screens |
ChiTaRSi | Tyrp1, mouse |
PROi | PR:P07147 |
RNActi | P07147, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000005994, Expressed in iris and 92 other tissues |
ExpressionAtlasi | P07147, baseline and differential |
Genevisiblei | P07147, MM |
Family and domain databases
Gene3Di | 1.10.1280.10, 1 hit |
InterProi | View protein in InterPro IPR002227, Tyrosinase_Cu-bd IPR008922, Unchr_di-copper_centre |
Pfami | View protein in Pfam PF00264, Tyrosinase, 1 hit |
PRINTSi | PR00092, TYROSINASE |
SUPFAMi | SSF48056, SSF48056, 1 hit |
PROSITEi | View protein in PROSITE PS00497, TYROSINASE_1, 1 hit PS00498, TYROSINASE_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TYRP1_MOUSE | |
Accessioni | P07147Primary (citable) accession number: P07147 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1988 |
Last sequence update: | April 1, 1988 | |
Last modified: | April 7, 2021 | |
This is version 180 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families