UniProtKB - P07143 (CY1_YEAST)
Cytochrome c1, heme protein, mitochondrial
CYT1
Functioni
Miscellaneous
Catalytic activityi
- 2 [Fe(III)cytochrome c](out) + a quinol = 2 [Fe(II)cytochrome c](out) + a quinone + 2 H+(out)1 PublicationEC:7.1.1.81 Publication
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 101 | Heme (covalent)Combined sources4 Publications | 1 | |
Binding sitei | 104 | Heme (covalent)Combined sources4 Publications | 1 | |
Metal bindingi | 105 | Iron (heme axial ligand); via tele nitrogenCombined sources3 Publications | 1 | |
Metal bindingi | 225 | Iron (heme axial ligand)Combined sources4 Publications | 1 |
GO - Molecular functioni
- electron transporter, transferring electrons within CoQH2-cytochrome c reductase complex activity Source: SGD
- heme binding Source: InterPro
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- mitochondrial ATP synthesis coupled proton transport Source: GO_Central
- mitochondrial electron transport, ubiquinol to cytochrome c Source: SGD
Keywordsi
Molecular function | Translocase |
Biological process | Electron transport, Respiratory chain, Transport |
Ligand | Heme, Iron, Metal-binding |
Enzyme and pathway databases
Reactomei | R-SCE-611105, Respiratory electron transport |
Protein family/group databases
TCDBi | 3.D.3.3.1, the proton-translocating quinol:cytochrome c reductase (qcr) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: Cytochrome c1, heme protein, mitochondrial (EC:7.1.1.81 Publication)Alternative name(s): Complex III subunit 4 Complex III subunit IV Cytochrome b-c1 complex subunit 4 Ubiquinol-cytochrome c oxidoreductase cytochrome c1 subunit Short name: Cytochrome c-1 |
Gene namesi | Name:CYT1 Synonyms:CTC1 Ordered Locus Names:YOR065W ORF Names:YOR29-16 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YOR065W |
SGDi | S000005591, CYT1 |
Subcellular locationi
Mitochondrion
- Mitochondrion inner membrane 2 Publications; Single-pass membrane protein 2 Publications
Cytosol
- cytosol Source: Reactome
Mitochondrion
- mitochondrial intermembrane space Source: Reactome
- mitochondrial respiratory chain complex III Source: SGD
- mitochondrion Source: SGD
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 62 – 262 | Mitochondrial intermembrane3 PublicationsAdd BLAST | 201 | |
Transmembranei | 263 – 296 | Helical2 PublicationsAdd BLAST | 34 | |
Topological domaini | 297 – 309 | Mitochondrial matrix3 PublicationsAdd BLAST | 13 |
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion inner membranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 166 | R → G: Abolishes catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 272 | K → A: Loss of RIP1 from the bc1 complex. | 1 | |
Mutagenesisi | 288 | K → L: Loss of CYT1 and COB from the bc1 complex; when associated with L-289 and L-296. 1 Publication | 1 | |
Mutagenesisi | 289 | K → L: Loss of CYT1 and COB from the bc1 complex; when associated with L-288 and L-296. 1 Publication | 1 | |
Mutagenesisi | 296 | K → L: Loss of CYT1 and COB from the bc1 complex; when associated with L-288 and L-289. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 61 | Mitochondrion2 PublicationsAdd BLAST | 61 | |
ChainiPRO_0000006566 | 62 – 309 | Cytochrome c1, heme protein, mitochondrialAdd BLAST | 248 |
Proteomic databases
MaxQBi | P07143 |
PaxDbi | P07143 |
PRIDEi | P07143 |
Interactioni
Subunit structurei
Component of the ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII), a multisubunit enzyme composed of 10 subunits. The complex is composed of 3 respiratory subunits cytochrome b (COB), cytochrome c1 (CYT1) and Rieske protein (RIP1), 2 core protein subunits COR1 and QCR2, and 5 low-molecular weight protein subunits QCR6, QCR7, QCR8, QCR9 and QCR10 (PubMed:10873857, PubMed:11880631, PubMed:18390544, PubMed:30598554). The complex exists as an obligatory dimer and forms supercomplexes (SCs) in the inner mitochondrial membrane with a monomer or a dimer of cytochrome c oxidase (complex IV, CIV), resulting in 2 different assemblies (supercomplexes III2IV and III2IV2) (PubMed:10775262, PubMed:10764779, PubMed:30598556, PubMed:30598554). CYT1 interacts with COX5A at the CIII-CIV interface (PubMed:30598554).
7 PublicationsProtein-protein interaction databases
BioGRIDi | 34464, 487 interactors |
ComplexPortali | CPX-567, Mitochondrial electron transport complex III |
DIPi | DIP-4166N |
IntActi | P07143, 15 interactors |
MINTi | P07143 |
STRINGi | 4932.YOR065W |
Miscellaneous databases
RNActi | P07143, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P07143 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P07143 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 88 – 241 | Cytochrome cPROSITE-ProRule annotationAdd BLAST | 154 |
Sequence similaritiesi
Keywords - Domaini
Transit peptide, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3052, Eukaryota |
GeneTreei | ENSGT00390000012445 |
HOGENOMi | CLU_040334_1_1_1 |
InParanoidi | P07143 |
OMAi | YQQVCAA |
Family and domain databases
Gene3Di | 1.10.760.10, 1 hit |
InterProi | View protein in InterPro IPR009056, Cyt_c-like_dom IPR036909, Cyt_c-like_dom_sf IPR002326, Cyt_c1 IPR021157, Cyt_c1_TM_anchor_C |
PANTHERi | PTHR10266, PTHR10266, 1 hit |
Pfami | View protein in Pfam PF02167, Cytochrom_C1, 1 hit |
PRINTSi | PR00603, CYTOCHROMEC1 |
SUPFAMi | SSF46626, SSF46626, 1 hit SSF81496, SSF81496, 1 hit |
PROSITEi | View protein in PROSITE PS51007, CYTC, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MFSNLSKRWA QRTLSKSFYS TATGAASKSG KLTQKLVTAG VAAAGITAST
60 70 80 90 100
LLYADSLTAE AMTAAEHGLH APAYAWSHNG PFETFDHASI RRGYQVYREV
110 120 130 140 150
CAACHSLDRV AWRTLVGVSH TNEEVRNMAE EFEYDDEPDE QGNPKKRPGK
160 170 180 190 200
LSDYIPGPYP NEQAARAANQ GALPPDLSLI VKARHGGCDY IFSLLTGYPD
210 220 230 240 250
EPPAGVALPP GSNYNPYFPG GSIAMARVLF DDMVEYEDGT PATTSQMAKD
260 270 280 290 300
VTTFLNWCAE PEHDERKRLG LKTVIILSSL YLLSIWVKKF KWAGIKTRKF
VFNPPKPRK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X00791 Genomic DNA Translation: CAA25375.1 Z74973 Genomic DNA Translation: CAA99258.1 Z70678 Genomic DNA Translation: CAA94550.1 BK006948 Genomic DNA Translation: DAA10844.1 |
PIRi | A22737, CCBY1H |
RefSeqi | NP_014708.1, NM_001183484.1 |
Genome annotation databases
EnsemblFungii | YOR065W_mRNA; YOR065W; YOR065W |
GeneIDi | 854231 |
KEGGi | sce:YOR065W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X00791 Genomic DNA Translation: CAA25375.1 Z74973 Genomic DNA Translation: CAA99258.1 Z70678 Genomic DNA Translation: CAA94550.1 BK006948 Genomic DNA Translation: DAA10844.1 |
PIRi | A22737, CCBY1H |
RefSeqi | NP_014708.1, NM_001183484.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1EZV | X-ray | 2.30 | D | 62-306 | [»] | |
1KB9 | X-ray | 2.30 | D | 62-307 | [»] | |
1KYO | X-ray | 2.97 | D/O | 62-309 | [»] | |
1P84 | X-ray | 2.50 | D | 62-307 | [»] | |
2IBZ | X-ray | 2.30 | D | 62-309 | [»] | |
3CX5 | X-ray | 1.90 | D/O | 62-309 | [»] | |
3CXH | X-ray | 2.50 | D/O | 62-309 | [»] | |
4PD4 | X-ray | 3.04 | D | 62-309 | [»] | |
6GIQ | electron microscopy | 3.23 | D/O | 1-309 | [»] | |
6HU9 | electron microscopy | 3.35 | D/O | 62-309 | [»] | |
6T0B | electron microscopy | 2.80 | D/O | 62-309 | [»] | |
6T15 | electron microscopy | 3.29 | D/O | 62-309 | [»] | |
6YMX | electron microscopy | 3.17 | D/O | 62-309 | [»] | |
SMRi | P07143 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 34464, 487 interactors |
ComplexPortali | CPX-567, Mitochondrial electron transport complex III |
DIPi | DIP-4166N |
IntActi | P07143, 15 interactors |
MINTi | P07143 |
STRINGi | 4932.YOR065W |
Protein family/group databases
TCDBi | 3.D.3.3.1, the proton-translocating quinol:cytochrome c reductase (qcr) superfamily |
Proteomic databases
MaxQBi | P07143 |
PaxDbi | P07143 |
PRIDEi | P07143 |
Genome annotation databases
EnsemblFungii | YOR065W_mRNA; YOR065W; YOR065W |
GeneIDi | 854231 |
KEGGi | sce:YOR065W |
Organism-specific databases
EuPathDBi | FungiDB:YOR065W |
SGDi | S000005591, CYT1 |
Phylogenomic databases
eggNOGi | KOG3052, Eukaryota |
GeneTreei | ENSGT00390000012445 |
HOGENOMi | CLU_040334_1_1_1 |
InParanoidi | P07143 |
OMAi | YQQVCAA |
Enzyme and pathway databases
Reactomei | R-SCE-611105, Respiratory electron transport |
Miscellaneous databases
EvolutionaryTracei | P07143 |
PROi | PR:P07143 |
RNActi | P07143, protein |
Family and domain databases
Gene3Di | 1.10.760.10, 1 hit |
InterProi | View protein in InterPro IPR009056, Cyt_c-like_dom IPR036909, Cyt_c-like_dom_sf IPR002326, Cyt_c1 IPR021157, Cyt_c1_TM_anchor_C |
PANTHERi | PTHR10266, PTHR10266, 1 hit |
Pfami | View protein in Pfam PF02167, Cytochrom_C1, 1 hit |
PRINTSi | PR00603, CYTOCHROMEC1 |
SUPFAMi | SSF46626, SSF46626, 1 hit SSF81496, SSF81496, 1 hit |
PROSITEi | View protein in PROSITE PS51007, CYTC, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CY1_YEAST | |
Accessioni | P07143Primary (citable) accession number: P07143 Secondary accession number(s): D6W2C8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1988 |
Last sequence update: | April 1, 1988 | |
Last modified: | December 2, 2020 | |
This is version 204 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast chromosome XV
Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD