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Protein

Macrophage colony-stimulating factor 1

Gene

Csf1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance.

Caution

The sequence reported in PubMed:8357831 was thought to originate from rat, but was later shown (PubMed:12074592 and PubMed:12379742) to be derived from mouse.Curated

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCytokine, Growth factor
Biological processImmunity, Inflammatory response, Innate immunity

Enzyme and pathway databases

ReactomeiR-MMU-381426 Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs)
R-MMU-449836 Other interleukin signaling
R-MMU-8957275 Post-translational protein phosphorylation

Names & Taxonomyi

Protein namesi
Recommended name:
Macrophage colony-stimulating factor 1
Short name:
CSF-1
Short name:
MCSF
Cleaved into the following chain:
Gene namesi
Name:Csf1
Synonyms:Csfm
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1339753 Csf1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini33 – 492ExtracellularSequence analysisAdd BLAST460
Transmembranei493 – 515HelicalSequence analysisAdd BLAST23
Topological domaini516 – 552CytoplasmicSequence analysisAdd BLAST37

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

Pathology & Biotechi

Involvement in diseasei

A defect in Csf1 is the cause of osteopetrosis. Osteopetrotic mice (op/op) are severely deficient in mature macrophages and osteoclasts, display failed tooth eruption, and have a restricted capacity for bone remodeling.1 Publication

Chemistry databases

ChEMBLiCHEMBL3638330

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 321 PublicationAdd BLAST32
ChainiPRO_000000585833 – 552Macrophage colony-stimulating factor 1Add BLAST520
ChainiPRO_000029623233 – 447Processed macrophage colony-stimulating factor 1By similarityAdd BLAST415

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi39 ↔ 1221 Publication
Disulfide bondi63Interchain1 Publication
Disulfide bondi80 ↔ 1711 Publication
Glycosylationi107N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi134 ↔ 1781 Publication
Glycosylationi154N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi172N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi189InterchainBy similarity
Disulfide bondi191InterchainBy similarity

Post-translational modificationi

N-glycosylated. The predominant soluble form is a chondroitin sulfate-containing proteoglycan.

Keywords - PTMi

Disulfide bond, Glycoprotein, Proteoglycan

Proteomic databases

PaxDbiP07141
PeptideAtlasiP07141
PRIDEiP07141

PTM databases

iPTMnetiP07141
PhosphoSitePlusiP07141
SwissPalmiP07141

Expressioni

Gene expression databases

BgeeiENSMUSG00000014599 Expressed in 268 organ(s), highest expression level in uterus
CleanExiMM_CSF1
ExpressionAtlasiP07141 baseline and differential
GenevisibleiP07141 MM

Interactioni

Subunit structurei

Homodimer or heterodimer; disulfide-linked. Interacts with CSF1R.1 Publication

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-45278N
IntActiP07141, 43 interactors
STRINGi10090.ENSMUSP00000014743

Chemistry databases

BindingDBiP07141

Structurei

Secondary structure

1552
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP07141
SMRiP07141
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07141

Family & Domainsi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410II8R Eukaryota
ENOG410Z5Q9 LUCA
GeneTreeiENSGT00390000015805
HOGENOMiHOG000112010
HOVERGENiHBG005410
InParanoidiP07141
KOiK05453
OMAiPMAGLTW
OrthoDBiEOG091G0TMC
PhylomeDBiP07141
TreeFamiTF337718

Family and domain databases

InterProiView protein in InterPro
IPR009079 4_helix_cytokine-like_core
IPR008001 MCSF-1
PANTHERiPTHR10058 PTHR10058, 1 hit
PfamiView protein in Pfam
PF05337 CSF-1, 1 hit
PIRSFiPIRSF001948 MCSF-1, 1 hit
SUPFAMiSSF47266 SSF47266, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P07141-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MTARGAAGRC PSSTWLGSRL LLVCLLMSRS IAKEVSEHCS HMIGNGHLKV
60 70 80 90 100
LQQLIDSQME TSCQIAFEFV DQEQLDDPVC YLKKAFFLVQ DIIDETMRFK
110 120 130 140 150
DNTPNANATE RLQELSNNLN SCFTKDYEEQ NKACVRTFHE TPLQLLEKIK
160 170 180 190 200
NFFNETKNLL EKDWNIFTKN CNNSFAKCSS RDVVTKPDCN CLYPKATPSS
210 220 230 240 250
DPASASPHQP PAPSMAPLAG LAWDDSQRTE GSSLLPSELP LRIEDPGSAK
260 270 280 290 300
QRPPRSTCQT LESTEQPNHG DRLTEDSQPH PSAGGPVPGV EDILESSLGT
310 320 330 340 350
NWVLEEASGE ASEGFLTQEA KFSPSTPVGG SIQAETDRPR ALSASPFPKS
360 370 380 390 400
TEDQKPVDIT DRPLTEVNPM RPIGQTQNNT PEKTDGTSTL REDHQEPGSP
410 420 430 440 450
HIATPNPQRV SNSATPVAQL LLPKSHSWGI VLPLGELEGK RSTRDRRSPA
460 470 480 490 500
ELEGGSASEG AARPVARFNS IPLTDTGHVE QHEGSSDPQI PESVFHLLVP
510 520 530 540 550
GIILVLLTVG GLLFYKWKWR SHRDPQTLDS SVGRPEDSSL TQDEDRQVEL

PV
Length:552
Mass (Da):60,649
Last modified:February 1, 1996 - v2
Checksum:i3886D72D70E770AF
GO
Isoform 2 (identifier: P07141-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     182-476: Missing.

Note: No experimental confirmation available.
Show »
Length:257
Mass (Da):29,353
Checksum:iE1EF2A20C416E591
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3Z090D3Z090_MOUSE
Macrophage colony-stimulating facto...
Csf1
182Annotation score:
D3YTW1D3YTW1_MOUSE
Macrophage colony-stimulating facto...
Csf1
163Annotation score:
F6RNW8F6RNW8_MOUSE
Macrophage colony-stimulating facto...
Csf1
278Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti3Missing in AAA37480 (PubMed:3493488).Curated1
Sequence conflicti6A → R in AAA37480 (PubMed:3493488).Curated1
Sequence conflicti7 – 8AG → PR in AAA37480 (PubMed:3493488).Curated2
Sequence conflicti246P → A in CAA28660 (PubMed:3494232).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti292D → G. 1
Natural varianti345S → P. 1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_001189182 – 476Missing in isoform 2. 1 PublicationAdd BLAST295

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05010 mRNA Translation: CAA28660.1
M21952 mRNA Translation: AAA37481.1
M21149 mRNA Translation: AAA37482.1
M84361 mRNA Translation: AAA03032.1
AK138489 mRNA Translation: BAE23681.1
AK154872 mRNA Translation: BAE32893.1
AK160995 mRNA Translation: BAE36140.1
CH466607 Genomic DNA Translation: EDL01916.1
CH466607 Genomic DNA Translation: EDL01919.1
BC025593 mRNA Translation: AAH25593.1
BC066187 mRNA Translation: AAH66187.1
BC066200 mRNA Translation: AAH66200.1
BC066205 mRNA Translation: AAH66205.1
M15692 mRNA Translation: AAA37480.1
M81316 Unassigned DNA Translation: AAA19866.1
CCDSiCCDS17740.1 [P07141-1]
CCDS51044.1 [P07141-2]
PIRiA31401
RefSeqiNP_001107001.1, NM_001113529.1 [P07141-2]
NP_001107002.1, NM_001113530.1 [P07141-1]
NP_031804.3, NM_007778.4 [P07141-1]
UniGeneiMm.795

Genome annotation databases

EnsembliENSMUST00000014743; ENSMUSP00000014743; ENSMUSG00000014599 [P07141-1]
ENSMUST00000118593; ENSMUSP00000113136; ENSMUSG00000014599 [P07141-2]
ENSMUST00000120243; ENSMUSP00000113617; ENSMUSG00000014599 [P07141-1]
GeneIDi12977
KEGGimmu:12977
UCSCiuc008qxk.2 mouse [P07141-1]
uc008qxl.2 mouse [P07141-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05010 mRNA Translation: CAA28660.1
M21952 mRNA Translation: AAA37481.1
M21149 mRNA Translation: AAA37482.1
M84361 mRNA Translation: AAA03032.1
AK138489 mRNA Translation: BAE23681.1
AK154872 mRNA Translation: BAE32893.1
AK160995 mRNA Translation: BAE36140.1
CH466607 Genomic DNA Translation: EDL01916.1
CH466607 Genomic DNA Translation: EDL01919.1
BC025593 mRNA Translation: AAH25593.1
BC066187 mRNA Translation: AAH66187.1
BC066200 mRNA Translation: AAH66200.1
BC066205 mRNA Translation: AAH66205.1
M15692 mRNA Translation: AAA37480.1
M81316 Unassigned DNA Translation: AAA19866.1
CCDSiCCDS17740.1 [P07141-1]
CCDS51044.1 [P07141-2]
PIRiA31401
RefSeqiNP_001107001.1, NM_001113529.1 [P07141-2]
NP_001107002.1, NM_001113530.1 [P07141-1]
NP_031804.3, NM_007778.4 [P07141-1]
UniGeneiMm.795

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3EJJX-ray2.40A/B36-180[»]
3UF5X-ray2.80A/B33-181[»]
4ADQX-ray4.50E/F/G/H33-181[»]
ProteinModelPortaliP07141
SMRiP07141
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-45278N
IntActiP07141, 43 interactors
STRINGi10090.ENSMUSP00000014743

Chemistry databases

BindingDBiP07141
ChEMBLiCHEMBL3638330

PTM databases

iPTMnetiP07141
PhosphoSitePlusiP07141
SwissPalmiP07141

Proteomic databases

PaxDbiP07141
PeptideAtlasiP07141
PRIDEiP07141

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000014743; ENSMUSP00000014743; ENSMUSG00000014599 [P07141-1]
ENSMUST00000118593; ENSMUSP00000113136; ENSMUSG00000014599 [P07141-2]
ENSMUST00000120243; ENSMUSP00000113617; ENSMUSG00000014599 [P07141-1]
GeneIDi12977
KEGGimmu:12977
UCSCiuc008qxk.2 mouse [P07141-1]
uc008qxl.2 mouse [P07141-2]

Organism-specific databases

CTDi1435
MGIiMGI:1339753 Csf1

Phylogenomic databases

eggNOGiENOG410II8R Eukaryota
ENOG410Z5Q9 LUCA
GeneTreeiENSGT00390000015805
HOGENOMiHOG000112010
HOVERGENiHBG005410
InParanoidiP07141
KOiK05453
OMAiPMAGLTW
OrthoDBiEOG091G0TMC
PhylomeDBiP07141
TreeFamiTF337718

Enzyme and pathway databases

ReactomeiR-MMU-381426 Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs)
R-MMU-449836 Other interleukin signaling
R-MMU-8957275 Post-translational protein phosphorylation

Miscellaneous databases

EvolutionaryTraceiP07141
PROiPR:P07141
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000014599 Expressed in 268 organ(s), highest expression level in uterus
CleanExiMM_CSF1
ExpressionAtlasiP07141 baseline and differential
GenevisibleiP07141 MM

Family and domain databases

InterProiView protein in InterPro
IPR009079 4_helix_cytokine-like_core
IPR008001 MCSF-1
PANTHERiPTHR10058 PTHR10058, 1 hit
PfamiView protein in Pfam
PF05337 CSF-1, 1 hit
PIRSFiPIRSF001948 MCSF-1, 1 hit
SUPFAMiSSF47266 SSF47266, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCSF1_MOUSE
AccessioniPrimary (citable) accession number: P07141
Secondary accession number(s): Q3U395, Q8R3C8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: February 1, 1996
Last modified: November 7, 2018
This is version 170 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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