UniProtKB - P07118 (SYV_ECOLI)
Protein
Valine--tRNA ligase
Gene
valS
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a 'posttransfer' editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner.
Catalytic activityi
- EC:6.1.1.9
Kineticsi
- KM=0.1 µM for tRNA1 Publication
- KM=47 µM for valine1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 557 | ATPBy similarity | 1 |
GO - Molecular functioni
- aminoacyl-tRNA editing activity Source: EcoCyc
- ATP binding Source: UniProtKB-UniRule
- magnesium ion binding Source: EcoCyc
- valine-tRNA ligase activity Source: EcoCyc
GO - Biological processi
- cytosolic valyl-tRNA aminoacylation Source: EcoCyc
- positive regulation of translational fidelity Source: EcoCyc
- valyl-tRNA aminoacylation Source: EcoCyc
Keywordsi
Molecular function | Aminoacyl-tRNA synthetase, Ligase |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:VALS-MONOMER MetaCyc:VALS-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Valine--tRNA ligase (EC:6.1.1.9)Alternative name(s): Valyl-tRNA synthetase Short name: ValRS |
Gene namesi | Name:valS Ordered Locus Names:b4258, JW4215 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
Cytosol
- cytosol Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 222 | T → P: Produces mischarged Thr-tRNA(Val) and Cys-tRNA(Val). 1 Publication | 1 | |
Mutagenesisi | 277 | K → A: Reduces posttransfer Thr-tRNA(Val) editing rate significantly and alters amino acid discrimination in the editing site, resulting in hydrolysis of the correctly charged cognate product. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000106224 | 1 – 951 | Valine--tRNA ligaseAdd BLAST | 951 |
Proteomic databases
jPOSTi | P07118 |
PaxDbi | P07118 |
PRIDEi | P07118 |
Interactioni
Subunit structurei
Monomer.
Protein-protein interaction databases
BioGRIDi | 4262724, 7 interactors 853071, 1 interactor |
DIPi | DIP-11112N |
IntActi | P07118, 6 interactors |
STRINGi | 511145.b4258 |
Family & Domainsi
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 880 – 944 | Sequence analysisAdd BLAST | 65 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 42 – 52 | 'HIGH' regionAdd BLAST | 11 | |
Motifi | 554 – 558 | 'KMSKS' region | 5 |
Domaini
ValRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated threonine is translocated from the active site to the editing site.
The C-terminal coiled-coil domain is crucial for aminoacylation activity.By similarity
Sequence similaritiesi
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | COG0525, Bacteria |
HOGENOMi | CLU_001493_0_2_6 |
InParanoidi | P07118 |
PhylomeDBi | P07118 |
Family and domain databases
CDDi | cd07962, Anticodon_Ia_Val, 1 hit |
Gene3Di | 1.10.287.380, 1 hit 3.40.50.620, 2 hits 3.90.740.10, 1 hit |
HAMAPi | MF_02004, Val_tRNA_synth_type1, 1 hit |
InterProi | View protein in InterPro IPR001412, aa-tRNA-synth_I_CS IPR002300, aa-tRNA-synth_Ia IPR033705, Anticodon_Ia_Val IPR013155, M/V/L/I-tRNA-synth_anticd-bd IPR014729, Rossmann-like_a/b/a_fold IPR010978, tRNA-bd_arm IPR009080, tRNAsynth_Ia_anticodon-bd IPR037118, Val-tRNA_synth_C_sf IPR019499, Val-tRNA_synth_tRNA-bd IPR009008, Val/Leu/Ile-tRNA-synth_edit IPR002303, Valyl-tRNA_ligase |
Pfami | View protein in Pfam PF08264, Anticodon_1, 1 hit PF00133, tRNA-synt_1, 1 hit PF10458, Val_tRNA-synt_C, 1 hit |
PRINTSi | PR00986, TRNASYNTHVAL |
SUPFAMi | SSF46589, SSF46589, 1 hit SSF47323, SSF47323, 1 hit SSF50677, SSF50677, 1 hit |
TIGRFAMsi | TIGR00422, valS, 1 hit |
PROSITEi | View protein in PROSITE PS00178, AA_TRNA_LIGASE_I, 1 hit |
i Sequence
Sequence statusi: Complete.
P07118-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEKTYNPQDI EQPLYEHWEK QGYFKPNGDE SQESFCIMIP PPNVTGSLHM
60 70 80 90 100
GHAFQQTIMD TMIRYQRMQG KNTLWQVGTD HAGIATQMVV ERKIAAEEGK
110 120 130 140 150
TRHDYGREAF IDKIWEWKAE SGGTITRQMR RLGNSVDWER ERFTMDEGLS
160 170 180 190 200
NAVKEVFVRL YKEDLIYRGK RLVNWDPKLR TAISDLEVEN RESKGSMWHI
210 220 230 240 250
RYPLADGAKT ADGKDYLVVA TTRPETLLGD TGVAVNPEDP RYKDLIGKYV
260 270 280 290 300
ILPLVNRRIP IVGDEHADME KGTGCVKITP AHDFNDYEVG KRHALPMINI
310 320 330 340 350
LTFDGDIRES AQVFDTKGNE SDVYSSEIPA EFQKLERFAA RKAVVAAVDA
360 370 380 390 400
LGLLEEIKPH DLTVPYGDRG GVVIEPMLTD QWYVRADVLA KPAVEAVENG
410 420 430 440 450
DIQFVPKQYE NMYFSWMRDI QDWCISRQLW WGHRIPAWYD EAGNVYVGRN
460 470 480 490 500
EDEVRKENNL GADVVLRQDE DVLDTWFSSA LWTFSTLGWP ENTDALRQFH
510 520 530 540 550
PTSVMVSGFD IIFFWIARMI MMTMHFIKDE NGKPQVPFHT VYMTGLIRDD
560 570 580 590 600
EGQKMSKSKG NVIDPLDMVD GISLPELLEK RTGNMMQPQL ADKIRKRTEK
610 620 630 640 650
QFPNGIEPHG TDALRFTLAA LASTGRDINW DMKRLEGYRN FCNKLWNASR
660 670 680 690 700
FVLMNTEGQD CGFNGGEMTL SLADRWILAE FNQTIKAYRE ALDSFRFDIA
710 720 730 740 750
AGILYEFTWN QFCDWYLELT KPVMNGGTEA ELRGTRHTLV TVLEGLLRLA
760 770 780 790 800
HPIIPFITET IWQRVKVLCG ITADTIMLQP FPQYDASQVD EAALADTEWL
810 820 830 840 850
KQAIVAVRNI RAEMNIAPGK PLELLLRGCS ADAERRVNEN RGFLQTLARL
860 870 880 890 900
ESITVLPADD KGPVSVTKII DGAELLIPMA GLINKEDELA RLAKEVAKIE
910 920 930 940 950
GEISRIENKL ANEGFVARAP EAVIAKEREK LEGYAEAKAK LIEQQAVIAA
L
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 107 | R → A in AAA24657 (PubMed:3275660).Curated | 1 | |
Sequence conflicti | 148 | G → D in AAP43521 (Ref. 6) Curated | 1 | |
Sequence conflicti | 452 | D → E in AAP43521 (Ref. 6) Curated | 1 | |
Sequence conflicti | 465 | V → A in AAP43521 (Ref. 6) Curated | 1 | |
Sequence conflicti | 694 | S → T in CAA29322 (PubMed:3317277).Curated | 1 | |
Sequence conflicti | 833 | A → R in AAA24657 (PubMed:3275660).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X05891 Genomic DNA Translation: CAA29322.1 J03497 Genomic DNA Translation: AAA24657.1 U14003 Genomic DNA Translation: AAA97155.1 U00096 Genomic DNA Translation: AAC77215.1 AP009048 Genomic DNA Translation: BAE78255.1 AY283771 Genomic DNA Translation: AAP43521.1 |
PIRi | E65238, SYECVT |
RefSeqi | NP_418679.1, NC_000913.3 WP_000416392.1, NZ_STEB01000013.1 |
Genome annotation databases
EnsemblBacteriai | AAC77215; AAC77215; b4258 BAE78255; BAE78255; BAE78255 |
GeneIDi | 57730692 948785 |
KEGGi | ecj:JW4215 eco:b4258 |
PATRICi | fig|1411691.4.peg.2446 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X05891 Genomic DNA Translation: CAA29322.1 J03497 Genomic DNA Translation: AAA24657.1 U14003 Genomic DNA Translation: AAA97155.1 U00096 Genomic DNA Translation: AAC77215.1 AP009048 Genomic DNA Translation: BAE78255.1 AY283771 Genomic DNA Translation: AAP43521.1 |
PIRi | E65238, SYECVT |
RefSeqi | NP_418679.1, NC_000913.3 WP_000416392.1, NZ_STEB01000013.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 4262724, 7 interactors 853071, 1 interactor |
DIPi | DIP-11112N |
IntActi | P07118, 6 interactors |
STRINGi | 511145.b4258 |
Proteomic databases
jPOSTi | P07118 |
PaxDbi | P07118 |
PRIDEi | P07118 |
Genome annotation databases
EnsemblBacteriai | AAC77215; AAC77215; b4258 BAE78255; BAE78255; BAE78255 |
GeneIDi | 57730692 948785 |
KEGGi | ecj:JW4215 eco:b4258 |
PATRICi | fig|1411691.4.peg.2446 |
Organism-specific databases
EchoBASEi | EB1060 |
Phylogenomic databases
eggNOGi | COG0525, Bacteria |
HOGENOMi | CLU_001493_0_2_6 |
InParanoidi | P07118 |
PhylomeDBi | P07118 |
Enzyme and pathway databases
BioCyci | EcoCyc:VALS-MONOMER MetaCyc:VALS-MONOMER |
Miscellaneous databases
PROi | PR:P07118 |
Family and domain databases
CDDi | cd07962, Anticodon_Ia_Val, 1 hit |
Gene3Di | 1.10.287.380, 1 hit 3.40.50.620, 2 hits 3.90.740.10, 1 hit |
HAMAPi | MF_02004, Val_tRNA_synth_type1, 1 hit |
InterProi | View protein in InterPro IPR001412, aa-tRNA-synth_I_CS IPR002300, aa-tRNA-synth_Ia IPR033705, Anticodon_Ia_Val IPR013155, M/V/L/I-tRNA-synth_anticd-bd IPR014729, Rossmann-like_a/b/a_fold IPR010978, tRNA-bd_arm IPR009080, tRNAsynth_Ia_anticodon-bd IPR037118, Val-tRNA_synth_C_sf IPR019499, Val-tRNA_synth_tRNA-bd IPR009008, Val/Leu/Ile-tRNA-synth_edit IPR002303, Valyl-tRNA_ligase |
Pfami | View protein in Pfam PF08264, Anticodon_1, 1 hit PF00133, tRNA-synt_1, 1 hit PF10458, Val_tRNA-synt_C, 1 hit |
PRINTSi | PR00986, TRNASYNTHVAL |
SUPFAMi | SSF46589, SSF46589, 1 hit SSF47323, SSF47323, 1 hit SSF50677, SSF50677, 1 hit |
TIGRFAMsi | TIGR00422, valS, 1 hit |
PROSITEi | View protein in PROSITE PS00178, AA_TRNA_LIGASE_I, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SYV_ECOLI | |
Accessioni | P07118Primary (citable) accession number: P07118 Secondary accession number(s): P78142, Q2M651, Q7X4V7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1988 |
Last sequence update: | November 1, 1997 | |
Last modified: | April 7, 2021 | |
This is version 185 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Aminoacyl-tRNA synthetases
List of aminoacyl-tRNA synthetase entries - SIMILARITY comments
Index of protein domains and families