UniProtKB - P07117 (PUTP_ECOLI)
Protein
Sodium/proline symporter
Gene
putP
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the sodium-dependent uptake of extracellular L-proline. This protein is also capable of using lithium as the transport cation. Also catalyzes the uptake of propionate.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 344 | Involved in high-affinity binding for sodium and proline | 1 |
GO - Molecular functioni
- proline:sodium symporter activity Source: EcoCyc
- sodium ion binding Source: InterPro
GO - Biological processi
- proline transmembrane transport Source: GO_Central
- proline transport Source: EcoCyc
- short-chain fatty acid transport Source: EcoCyc
Keywordsi
Biological process | Amino-acid transport, Ion transport, Sodium transport, Symport, Transport |
Ligand | Sodium |
Enzyme and pathway databases
BioCyci | EcoCyc:PUTP-MONOMER MetaCyc:PUTP-MONOMER |
Protein family/group databases
TCDBi | 2.A.21.2.1, the solute:sodium symporter (sss) family |
Names & Taxonomyi
Protein namesi | Recommended name: Sodium/proline symporterAlternative name(s): Proline permease Propionate transporter |
Gene namesi | Name:putP Ordered Locus Names:b1015, JW1001 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell inner membrane 1 Publication; Multi-pass membrane protein 1 Publication
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 3 | CytoplasmicSequence analysis | 3 | |
Transmembranei | 4 – 26 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 27 – 66 | PeriplasmicSequence analysisAdd BLAST | 40 | |
Transmembranei | 67 – 87 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 88 – 124 | CytoplasmicSequence analysisAdd BLAST | 37 | |
Transmembranei | 125 – 150 | HelicalSequence analysisAdd BLAST | 26 | |
Topological domaini | 151 – 162 | PeriplasmicSequence analysisAdd BLAST | 12 | |
Transmembranei | 163 – 184 | HelicalSequence analysisAdd BLAST | 22 | |
Topological domaini | 185 – 189 | CytoplasmicSequence analysis | 5 | |
Transmembranei | 190 – 213 | HelicalSequence analysisAdd BLAST | 24 | |
Topological domaini | 214 – 231 | PeriplasmicSequence analysisAdd BLAST | 18 | |
Transmembranei | 232 – 248 | HelicalSequence analysisAdd BLAST | 17 | |
Topological domaini | 249 – 274 | CytoplasmicSequence analysisAdd BLAST | 26 | |
Transmembranei | 275 – 295 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 296 – 319 | PeriplasmicSequence analysisAdd BLAST | 24 | |
Transmembranei | 320 – 340 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 341 – 370 | CytoplasmicSequence analysisAdd BLAST | 30 | |
Transmembranei | 371 – 391 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 392 – 397 | PeriplasmicSequence analysis | 6 | |
Transmembranei | 398 – 423 | HelicalSequence analysisAdd BLAST | 26 | |
Topological domaini | 424 – 430 | CytoplasmicSequence analysis | 7 | |
Transmembranei | 431 – 445 | HelicalSequence analysisAdd BLAST | 15 | |
Topological domaini | 446 – 448 | PeriplasmicSequence analysis | 3 | |
Transmembranei | 449 – 475 | HelicalSequence analysisAdd BLAST | 27 | |
Topological domaini | 476 – 502 | CytoplasmicSequence analysisAdd BLAST | 27 |
GO - Cellular componenti
- integral component of plasma membrane Source: EcoCyc
- plasma membrane Source: EcoCyc
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Reduced growth rate when grown on propionate as sole carbon source.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 257 | R → C: Sodium-independent binding affinity for proline. | 1 | |
Mutagenesisi | 376 | R → E or Q: No change in activity. 1 Publication | 1 | |
Mutagenesisi | 376 | R → K: Loss of activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000105398 | 1 – 502 | Sodium/proline symporterAdd BLAST | 502 |
Proteomic databases
PaxDbi | P07117 |
PRIDEi | P07117 |
Expressioni
Inductioni
Up-regulated by propionate.1 Publication
Interactioni
Subunit structurei
Has been isolated from inner membrane preparations as a homodimer.
1 PublicationProtein-protein interaction databases
BioGRIDi | 4260051, 14 interactors 849976, 3 interactors |
IntActi | P07117, 4 interactors |
STRINGi | 511145.b1015 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 27 – 66 | HydrophilicAdd BLAST | 40 | |
Regioni | 88 – 124 | HydrophilicAdd BLAST | 37 | |
Regioni | 151 – 162 | HydrophilicAdd BLAST | 12 | |
Regioni | 185 – 189 | Hydrophilic | 5 | |
Regioni | 214 – 231 | HydrophilicAdd BLAST | 18 | |
Regioni | 249 – 274 | HydrophilicAdd BLAST | 26 | |
Regioni | 296 – 319 | HydrophilicAdd BLAST | 24 | |
Regioni | 341 – 370 | HydrophilicAdd BLAST | 30 | |
Regioni | 392 – 397 | Hydrophilic | 6 | |
Regioni | 424 – 430 | Hydrophilic | 7 | |
Regioni | 446 – 448 | Hydrophilic | 3 | |
Regioni | 476 – 502 | HydrophilicAdd BLAST | 27 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG0591, Bacteria |
HOGENOMi | CLU_018808_15_2_6 |
InParanoidi | P07117 |
PhylomeDBi | P07117 |
Family and domain databases
CDDi | cd11475, SLC5sbd_PutP, 1 hit |
Gene3Di | 1.20.1730.10, 1 hit |
InterProi | View protein in InterPro IPR038377, Na/Glc_symporter_sf IPR011851, Na/Pro_symporter IPR001734, Na/solute_symporter IPR018212, Na/solute_symporter_CS |
Pfami | View protein in Pfam PF00474, SSF, 1 hit |
TIGRFAMsi | TIGR02121, Na_Pro_sym, 1 hit TIGR00813, sss, 1 hit |
PROSITEi | View protein in PROSITE PS00456, NA_SOLUT_SYMP_1, 1 hit PS00457, NA_SOLUT_SYMP_2, 1 hit PS50283, NA_SOLUT_SYMP_3, 1 hit |
i Sequence
Sequence statusi: Complete.
P07117-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAISTPMLVT FCVYIFGMIL IGFIAWRSTK NFDDYILGGR SLGPFVTALS
60 70 80 90 100
AGASDMSGWL LMGLPGAVFL SGISESWIAI GLTLGAWINW KLVAGRLRVH
110 120 130 140 150
TEYNNNALTL PDYFTGRFED KSRILRIISA LVILLFFTIY CASGIVAGAR
160 170 180 190 200
LFESTFGMSY ETALWAGAAA TILYTFIGGF LAVSWTDTVQ ASLMIFALIL
210 220 230 240 250
TPVIVIISVG GFGDSLEVIK QKSIENVDML KGLNFVAIIS LMGWGLGYFG
260 270 280 290 300
QPHILARFMA ADSHHSIVHA RRISMTWMIL CLAGAVAVGF FGIAYFNDHP
310 320 330 340 350
ALAGAVNQNA ERVFIELAQI LFNPWIAGIL LSAILAAVMS TLSCQLLVCS
360 370 380 390 400
SAITEDLYKA FLRKHASQKE LVWVGRVMVL VVALVAIALA ANPENRVLGL
410 420 430 440 450
VSYAWAGFGA AFGPVVLFSV MWSRMTRNGA LAGMIIGALT VIVWKQFGWL
460 470 480 490 500
GLYEIIPGFI FGSIGIVVFS LLGKAPSAAM QKRFAEADAH YHSAPPSRLQ
ES
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X05653 Genomic DNA Translation: CAA29143.1 U00096 Genomic DNA Translation: AAC74100.1 AP009048 Genomic DNA Translation: BAA35793.1 L01150 Genomic DNA Translation: AAA24463.1 L01151 Genomic DNA Translation: AAA24464.1 M35174 Genomic DNA Translation: AAA24458.1 |
PIRi | A30258, JGECPP |
RefSeqi | NP_415535.1, NC_000913.3 WP_001018479.1, NZ_SSZK01000002.1 |
Genome annotation databases
EnsemblBacteriai | AAC74100; AAC74100; b1015 BAA35793; BAA35793; BAA35793 |
GeneIDi | 945602 |
KEGGi | ecj:JW1001 eco:b1015 |
PATRICi | fig|1411691.4.peg.1254 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X05653 Genomic DNA Translation: CAA29143.1 U00096 Genomic DNA Translation: AAC74100.1 AP009048 Genomic DNA Translation: BAA35793.1 L01150 Genomic DNA Translation: AAA24463.1 L01151 Genomic DNA Translation: AAA24464.1 M35174 Genomic DNA Translation: AAA24458.1 |
PIRi | A30258, JGECPP |
RefSeqi | NP_415535.1, NC_000913.3 WP_001018479.1, NZ_SSZK01000002.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 4260051, 14 interactors 849976, 3 interactors |
IntActi | P07117, 4 interactors |
STRINGi | 511145.b1015 |
Protein family/group databases
TCDBi | 2.A.21.2.1, the solute:sodium symporter (sss) family |
Proteomic databases
PaxDbi | P07117 |
PRIDEi | P07117 |
Genome annotation databases
EnsemblBacteriai | AAC74100; AAC74100; b1015 BAA35793; BAA35793; BAA35793 |
GeneIDi | 945602 |
KEGGi | ecj:JW1001 eco:b1015 |
PATRICi | fig|1411691.4.peg.1254 |
Organism-specific databases
EchoBASEi | EB0795 |
Phylogenomic databases
eggNOGi | COG0591, Bacteria |
HOGENOMi | CLU_018808_15_2_6 |
InParanoidi | P07117 |
PhylomeDBi | P07117 |
Enzyme and pathway databases
BioCyci | EcoCyc:PUTP-MONOMER MetaCyc:PUTP-MONOMER |
Miscellaneous databases
PROi | PR:P07117 |
Family and domain databases
CDDi | cd11475, SLC5sbd_PutP, 1 hit |
Gene3Di | 1.20.1730.10, 1 hit |
InterProi | View protein in InterPro IPR038377, Na/Glc_symporter_sf IPR011851, Na/Pro_symporter IPR001734, Na/solute_symporter IPR018212, Na/solute_symporter_CS |
Pfami | View protein in Pfam PF00474, SSF, 1 hit |
TIGRFAMsi | TIGR02121, Na_Pro_sym, 1 hit TIGR00813, sss, 1 hit |
PROSITEi | View protein in PROSITE PS00456, NA_SOLUT_SYMP_1, 1 hit PS00457, NA_SOLUT_SYMP_2, 1 hit PS50283, NA_SOLUT_SYMP_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PUTP_ECOLI | |
Accessioni | P07117Primary (citable) accession number: P07117 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1988 |
Last sequence update: | April 1, 1988 | |
Last modified: | December 2, 2020 | |
This is version 172 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families