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Protein

Regulatory protein SdiA

Gene

sdiA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Activates cell division by specifically increasing transcription from one of the two promoters that lie immediately upstream of the ftsQAZ gene cluster. Activates ydiV expression in response to extracellular autoinducer AI-1 (Vibrio fischeri autoinducer oxoC6).2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi197 – 216H-T-H motifPROSITE-ProRule annotationAdd BLAST20

GO - Molecular functioni

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cell division Source: UniProtKB-KW
  • positive regulation of cytokinesis Source: EcoCyc
  • positive regulation of DNA-templated transcription, initiation Source: EcoCyc
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActivator, DNA-binding
Biological processCell cycle, Cell division, Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciEcoCyc:PD02198

Names & Taxonomyi

Protein namesi
Recommended name:
Regulatory protein SdiA
Gene namesi
Name:sdiA
Ordered Locus Names:b1916, JW1901
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10935 sdiA

Pathology & Biotechi

Disruption phenotypei

A double sdiA/ydiV deletion mutant leads to decreased cAMP levels which inhibits quorum sensing system 2.1 Publication

Chemistry databases

DrugBankiDB07928 N-(2-OXOTETRAHYDROFURAN-3-YL)OCTANAMIDE

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001841871 – 240Regulatory protein SdiAAdd BLAST240

Proteomic databases

PaxDbiP07026
PRIDEiP07026

Expressioni

Inductioni

Repressed by glucose.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4259602, 108 interactors
IntActiP07026, 8 interactors
STRINGi316385.ECDH10B_2057

Structurei

Secondary structure

1240
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 19Combined sources14
Helixi23 – 36Combined sources14
Beta strandi40 – 47Combined sources8
Beta strandi51 – 54Combined sources4
Beta strandi57 – 61Combined sources5
Helixi65 – 73Combined sources9
Helixi76 – 78Combined sources3
Helixi81 – 83Combined sources3
Helixi85 – 87Combined sources3
Beta strandi92 – 94Combined sources3
Helixi97 – 100Combined sources4
Helixi104 – 112Combined sources9
Beta strandi117 – 124Combined sources8
Beta strandi130 – 138Combined sources9
Helixi147 – 167Combined sources21
Turni171 – 173Combined sources3
Helixi182 – 192Combined sources11
Helixi197 – 204Combined sources8
Helixi208 – 222Combined sources15
Helixi227 – 236Combined sources10

3D structure databases

ProteinModelPortaliP07026
SMRiP07026
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07026

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini173 – 238HTH luxR-typePROSITE-ProRule annotationAdd BLAST66

Domaini

Binding to the autoinducer occurs via the N-terminal 170 residues; as E.coli does not produce LuxI autoinducers endogenously it should be able to bind to a number of different AI-1 autoinducers, which would enable it to detect other bacteria. This was shown to be the case.

Phylogenomic databases

eggNOGiENOG4105VJY Bacteria
COG2771 LUCA
HOGENOMiHOG000111053
InParanoidiP07026
KOiK07782
OMAiNHTVDWY
PhylomeDBiP07026

Family and domain databases

CDDicd06170 LuxR_C_like, 1 hit
Gene3Di1.10.10.10, 1 hit
3.30.450.80, 1 hit
InterProiView protein in InterPro
IPR016032 Sig_transdc_resp-reg_C-effctor
IPR005143 TF_LuxR_autoind-bd_dom
IPR036693 TF_LuxR_autoind-bd_dom_sf
IPR000792 Tscrpt_reg_LuxR_C
IPR036388 WH-like_DNA-bd_sf
PfamiView protein in Pfam
PF03472 Autoind_bind, 1 hit
PF00196 GerE, 1 hit
PRINTSiPR00038 HTHLUXR
SMARTiView protein in SMART
SM00421 HTH_LUXR, 1 hit
SUPFAMiSSF46894 SSF46894, 1 hit
SSF75516 SSF75516, 1 hit
PROSITEiView protein in PROSITE
PS00622 HTH_LUXR_1, 1 hit
PS50043 HTH_LUXR_2, 1 hit

Sequencei

Sequence statusi: Complete.

P07026-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQDKDFFSWR RTMLLRFQRM ETAEEVYHEI ELQAQQLEYD YYSLCVRHPV
60 70 80 90 100
PFTRPKVAFY TNYPEAWVSY YQAKNFLAID PVLNPENFSQ GHLMWNDDLF
110 120 130 140 150
SEAQPLWEAA RAHGLRRGVT QYLMLPNRAL GFLSFSRCSA REIPILSDEL
160 170 180 190 200
QLKMQLLVRE SLMALMRLND EIVMTPEMNF SKREKEILRW TAEGKTSAEI
210 220 230 240
AMILSISENT VNFHQKNMQK KINAPNKTQV ACYAAATGLI
Length:240
Mass (Da):28,117
Last modified:November 1, 1997 - v2
Checksum:iC04CCC50C06135C4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti120 – 128TQYLMLPNR → HSVFNAAQTG in CAA27327 (PubMed:3515318).Curated9
Sequence conflicti140A → R in CAA27327 (PubMed:3515318).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03691 Genomic DNA Translation: CAA27327.1
U00096 Genomic DNA Translation: AAC74983.1
AP009048 Genomic DNA Translation: BAA15736.1
PIRiA64955 QQECU1
RefSeqiNP_416426.1, NC_000913.3
WP_001152715.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74983; AAC74983; b1916
BAA15736; BAA15736; BAA15736
GeneIDi946421
KEGGiecj:JW1901
eco:b1916
PATRICifig|1411691.4.peg.333

Similar proteinsi

Entry informationi

Entry nameiSDIA_ECOLI
AccessioniPrimary (citable) accession number: P07026
Secondary accession number(s): P76313
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 141 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

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