UniProtKB - P07018 (MCP4_ECOLI)
Protein
Methyl-accepting chemotaxis protein IV
Gene
tap
Organism
Escherichia coli (strain K12)
Status
Functioni
Mediates taxis toward dipeptides via an interaction with the periplasmic dipeptide-binding protein.
Chemotactic-signal transducers respond to changes in the concentration of attractants and repellents in the environment, transduce a signal from the outside to the inside of the cell, and facilitate sensory adaptation through the variation of the level of methylation. Attractants increase the level of methylation while repellents decrease the level of methylation, the methyl groups are added by the methyltransferase CheR and removed by the methylesterase CheB.
GO - Molecular functioni
- transmembrane signaling receptor activity Source: EcoCyc
GO - Biological processi
- chemotaxis Source: EcoCyc
- signal transduction Source: UniProtKB-KW
Keywordsi
Molecular function | Transducer |
Biological process | Chemotaxis |
Enzyme and pathway databases
BioCyci | EcoCyc:TAP-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Methyl-accepting chemotaxis protein IVShort name: MCP-IV Alternative name(s): Dipeptide chemoreceptor protein |
Gene namesi | Name:tap Ordered Locus Names:b1885, JW1874 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane 1 Publication; Multi-pass membrane protein 1 Publication
Note: Found predominantly at cell poles.
Plasma Membrane
- integral component of plasma membrane Source: GO_Central
- plasma membrane Source: EcoCyc
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 6 | CytoplasmicSequence analysis | 6 | |
Transmembranei | 7 – 33 | HelicalSequence analysisAdd BLAST | 27 | |
Topological domaini | 34 – 188 | PeriplasmicSequence analysisAdd BLAST | 155 | |
Transmembranei | 189 – 209 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 210 – 533 | CytoplasmicSequence analysisAdd BLAST | 324 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000110541 | 1 – 533 | Methyl-accepting chemotaxis protein IVAdd BLAST | 533 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 293 | Glutamate methyl ester (Gln)By similarity | 1 | |
Modified residuei | 300 | Glutamate methyl ester (Gln)By similarity | 1 | |
Modified residuei | 307 | Glutamate methyl ester (Gln)By similarity | 1 | |
Modified residuei | 489 | Glutamate methyl ester (Glu)By similarity | 1 |
Keywords - PTMi
MethylationProteomic databases
PaxDbi | P07018 |
PRIDEi | P07018 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4262072, 172 interactors |
DIPi | DIP-10955N |
IntActi | P07018, 2 interactors |
STRINGi | 511145.b1885 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 212 – 264 | HAMPPROSITE-ProRule annotationAdd BLAST | 53 | |
Domaini | 269 – 498 | Methyl-accepting transducerPROSITE-ProRule annotationAdd BLAST | 230 |
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG0840, Bacteria |
HOGENOMi | CLU_000445_107_16_6 |
InParanoidi | P07018 |
PhylomeDBi | P07018 |
Family and domain databases
CDDi | cd00181, Tar_Tsr_LBD, 1 hit |
InterProi | View protein in InterPro IPR035440, 4HB_MCP_dom_sf IPR004090, Chemotax_Me-accpt_rcpt IPR004091, Chemotax_Me-accpt_rcpt_Me-site IPR003660, HAMP_dom IPR004089, MCPsignal_dom IPR003122, Tar_rcpt_lig-bd |
Pfami | View protein in Pfam PF00672, HAMP, 1 hit PF00015, MCPsignal, 1 hit PF02203, TarH, 1 hit |
PRINTSi | PR00260, CHEMTRNSDUCR |
SMARTi | View protein in SMART SM00304, HAMP, 1 hit SM00283, MA, 1 hit SM00319, TarH, 1 hit |
SUPFAMi | SSF47170, SSF47170, 1 hit |
PROSITEi | View protein in PROSITE PS00538, CHEMOTAXIS_TRANSDUC_1, 1 hit PS50111, CHEMOTAXIS_TRANSDUC_2, 1 hit PS50885, HAMP, 1 hit |
i Sequence
Sequence statusi: Complete.
P07018-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MFNRIRISTT LFLILILCGI LQIGSNGMSF WAFRDDLQRL NQVEQSNQQR
60 70 80 90 100
AALAQTRAVM LQASTALNKA GTLTALSYPA DDIKTLMTTA RASLTQSTTL
110 120 130 140 150
FKSFMAMTAG NEHVRGLQKE TEKSFARWHN DLEHQATWLE SNQLSDFLTA
160 170 180 190 200
PVQGSQNAFD VNFEAWQLEI NHVLEAASAQ SQRNYQISAL VFISMIIVAA
210 220 230 240 250
IYISSALWWT RKMIVQPLAI IGSHFDSIAA GNLARPIAVY GRNEITAIFA
260 270 280 290 300
SLKTMQQALR GTVSDVRKGS QEMHIGIAEI VAGNNDLSSR TEQQAASLAQ
310 320 330 340 350
TAASMEQLTA TVGQNADNAR QASELAKNAA TTAQAGGVQV STMTHTMQEI
360 370 380 390 400
ATSSQKIGDI ISVIDGIAFQ TNILALNAAV EAARAGEQGR GFAVVAGEVR
410 420 430 440 450
NLASRSAQAA KEIKGLIEES VNRVQQGSKL VNNAAATMID IVSSVTRVND
460 470 480 490 500
IMGEIASASE EQQRGIEQVA QAVSQMDQVT QQNASLVEEA AVATEQLANQ
510 520 530
ADHLSSRVAV FTLEEHEVAR HESVQLQIAP VVS
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 335 | A → G in AAA23567 (PubMed:6305515).Curated | 1 | |
Sequence conflicti | 503 | H → R in AAA23567 (PubMed:6305515).Curated | 1 | |
Sequence conflicti | 527 – 533 | QIAPVVS → TNCASGILK in AAA23567 (PubMed:6305515).Curated | 7 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J01705 Genomic DNA Translation: AAA23567.1 U00096 Genomic DNA Translation: AAC74955.1 AP009048 Genomic DNA Translation: BAA15701.1 |
PIRi | E64951, QRECM2 |
RefSeqi | NP_416399.1, NC_000913.3 WP_000483239.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC74955; AAC74955; b1885 BAA15701; BAA15701; BAA15701 |
GeneIDi | 946397 |
KEGGi | ecj:JW1874 eco:b1885 |
PATRICi | fig|1411691.4.peg.362 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J01705 Genomic DNA Translation: AAA23567.1 U00096 Genomic DNA Translation: AAC74955.1 AP009048 Genomic DNA Translation: BAA15701.1 |
PIRi | E64951, QRECM2 |
RefSeqi | NP_416399.1, NC_000913.3 WP_000483239.1, NZ_LN832404.1 |
3D structure databases
SMRi | P07018 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4262072, 172 interactors |
DIPi | DIP-10955N |
IntActi | P07018, 2 interactors |
STRINGi | 511145.b1885 |
Proteomic databases
PaxDbi | P07018 |
PRIDEi | P07018 |
Genome annotation databases
EnsemblBacteriai | AAC74955; AAC74955; b1885 BAA15701; BAA15701; BAA15701 |
GeneIDi | 946397 |
KEGGi | ecj:JW1874 eco:b1885 |
PATRICi | fig|1411691.4.peg.362 |
Organism-specific databases
EchoBASEi | EB0980 |
Phylogenomic databases
eggNOGi | COG0840, Bacteria |
HOGENOMi | CLU_000445_107_16_6 |
InParanoidi | P07018 |
PhylomeDBi | P07018 |
Enzyme and pathway databases
BioCyci | EcoCyc:TAP-MONOMER |
Miscellaneous databases
PROi | PR:P07018 |
Family and domain databases
CDDi | cd00181, Tar_Tsr_LBD, 1 hit |
InterProi | View protein in InterPro IPR035440, 4HB_MCP_dom_sf IPR004090, Chemotax_Me-accpt_rcpt IPR004091, Chemotax_Me-accpt_rcpt_Me-site IPR003660, HAMP_dom IPR004089, MCPsignal_dom IPR003122, Tar_rcpt_lig-bd |
Pfami | View protein in Pfam PF00672, HAMP, 1 hit PF00015, MCPsignal, 1 hit PF02203, TarH, 1 hit |
PRINTSi | PR00260, CHEMTRNSDUCR |
SMARTi | View protein in SMART SM00304, HAMP, 1 hit SM00283, MA, 1 hit SM00319, TarH, 1 hit |
SUPFAMi | SSF47170, SSF47170, 1 hit |
PROSITEi | View protein in PROSITE PS00538, CHEMOTAXIS_TRANSDUC_1, 1 hit PS50111, CHEMOTAXIS_TRANSDUC_2, 1 hit PS50885, HAMP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MCP4_ECOLI | |
Accessioni | P07018Primary (citable) accession number: P07018 Secondary accession number(s): P76300 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1988 |
Last sequence update: | November 1, 1997 | |
Last modified: | April 7, 2021 | |
This is version 173 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |