UniProtKB - P07004 (PROA_ECOLI)
Protein
Gamma-glutamyl phosphate reductase
Gene
proA
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the NADPH-dependent reduction of L-glutamate 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate.UniRule annotation2 Publications
Catalytic activityi
- L-glutamate 5-semialdehyde + NADP+ + phosphate = H+ + L-glutamyl 5-phosphate + NADPHUniRule annotation2 PublicationsEC:1.2.1.41UniRule annotation2 Publications
Activity regulationi
Inhibited by p-chloromercuribenzoate, copper and nickel ions.1 Publication
pH dependencei
Optimum pH is 7.0.1 Publication
: L-proline biosynthesis Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate.UniRule annotation1 PublicationProteins known to be involved in the 2 steps of the subpathway in this organism are:
- Glutamate 5-kinase (proB), Glutamate 5-kinase (proB)
- Gamma-glutamyl phosphate reductase (proA), Gamma-glutamyl phosphate reductase (proA)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.
GO - Molecular functioni
- glutamate-5-semialdehyde dehydrogenase activity Source: EcoCyc
- identical protein binding Source: EcoCyc
- NADP binding Source: InterPro
GO - Biological processi
- L-proline biosynthetic process Source: EcoCyc
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Amino-acid biosynthesis, Proline biosynthesis |
Ligand | NADP |
Enzyme and pathway databases
BioCyci | EcoCyc:GLUTSEMIALDEHYDROG-MONOMER MetaCyc:GLUTSEMIALDEHYDROG-MONOMER |
UniPathwayi | UPA00098;UER00360 |
Names & Taxonomyi
Protein namesi | Recommended name: Gamma-glutamyl phosphate reductaseUniRule annotation (EC:1.2.1.41UniRule annotation)Short name: GPRUniRule annotation Alternative name(s): Glutamate-5-semialdehyde dehydrogenaseUniRule annotation Glutamyl-gamma-semialdehyde dehydrogenaseUniRule annotation Short name: GSA dehydrogenaseUniRule annotation |
Gene namesi | Name:proAUniRule annotation Ordered Locus Names:b0243, JW0233 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation
Cytosol
- cytosol Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000189723 | 1 – 417 | Gamma-glutamyl phosphate reductaseAdd BLAST | 417 |
Proteomic databases
jPOSTi | P07004 |
PaxDbi | P07004 |
PRIDEi | P07004 |
2D gel databases
SWISS-2DPAGEi | P07004 |
Interactioni
Subunit structurei
Homodimer (Probable). May form a complex with glutamate 5-kinase (proB).
Curated2 PublicationsBinary interactionsi
Hide detailsP07004
With | #Exp. | IntAct |
---|---|---|
btsR [P0AFT5] | 4 | EBI-548584,EBI-556431 |
cysA [P16676] | 3 | EBI-548584,EBI-556404 |
sfsA [P0A823] | 4 | EBI-548584,EBI-556413 |
GO - Molecular functioni
- identical protein binding Source: EcoCyc
Protein-protein interaction databases
BioGRIDi | 4259768, 49 interactors 851022, 4 interactors |
DIPi | DIP-10568N |
IntActi | P07004, 17 interactors |
STRINGi | 511145.b0243 |
Family & Domainsi
Sequence similaritiesi
Belongs to the gamma-glutamyl phosphate reductase family.UniRule annotation
Phylogenomic databases
eggNOGi | COG0014, Bacteria |
HOGENOMi | CLU_030231_0_0_6 |
InParanoidi | P07004 |
PhylomeDBi | P07004 |
Family and domain databases
CDDi | cd07079, ALDH_F18-19_ProA-GPR, 1 hit |
Gene3Di | 3.40.309.10, 1 hit 3.40.605.10, 1 hit |
HAMAPi | MF_00412, ProA, 1 hit |
InterProi | View protein in InterPro IPR016161, Ald_DH/histidinol_DH IPR016163, Ald_DH_C IPR016162, Ald_DH_N IPR015590, Aldehyde_DH_dom IPR020593, G-glutamylP_reductase_CS IPR012134, Glu-5-SA_DH IPR000965, GPR_dom |
Pfami | View protein in Pfam PF00171, Aldedh, 1 hit |
PIRSFi | PIRSF000151, GPR, 1 hit |
SUPFAMi | SSF53720, SSF53720, 1 hit |
TIGRFAMsi | TIGR00407, proA, 1 hit |
PROSITEi | View protein in PROSITE PS01223, PROA, 1 hit |
i Sequence
Sequence statusi: Complete.
P07004-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLEQMGIAAK QASYKLAQLS SREKNRVLEK IADELEAQSE IILNANAQDV
60 70 80 90 100
ADARANGLSE AMLDRLALTP ARLKGIADDV RQVCNLADPV GQVIDGGVLD
110 120 130 140 150
SGLRLERRRV PLGVIGVIYE ARPNVTVDVA SLCLKTGNAV ILRGGKETCR
160 170 180 190 200
TNAATVAVIQ DALKSCGLPA GAVQAIDNPD RALVSEMLRM DKYIDMLIPR
210 220 230 240 250
GGAGLHKLCR EQSTIPVITG GIGVCHIYVD ESVEIAEALK VIVNAKTQRP
260 270 280 290 300
STCNTVETLL VNKNIADSFL PALSKQMAES GVTLHADAAA LAQLQAGPAK
310 320 330 340 350
VVAVKAEEYD DEFLSLDLNV KIVSDLDDAI AHIREHGTQH SDAILTRDMR
360 370 380 390 400
NAQRFVNEVD SSAVYVNAST RFTDGGQFGL GAEVAVSTQK LHARGPMGLE
410
ALTTYKWIGI GDYTIRA
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 358 – 367 | Missing (PubMed:6089111).Curated | 10 | |
Sequence conflicti | 358 – 367 | Missing (Ref. 2) Curated | 10 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X00786 Genomic DNA Translation: CAA25364.1 U70214 Genomic DNA Translation: AAB08663.1 U00096 Genomic DNA Translation: AAC73347.1 AP009048 Genomic DNA Translation: BAA77912.2 Z12832 Genomic DNA Translation: CAA78292.1 |
PIRi | D64749, RDECER |
RefSeqi | NP_414778.1, NC_000913.3 WP_000893278.1, NZ_SSZK01000050.1 |
Genome annotation databases
EnsemblBacteriai | AAC73347; AAC73347; b0243 BAA77912; BAA77912; BAA77912 |
GeneIDi | 57728932 946680 |
KEGGi | ecj:JW0233 eco:b0243 |
PATRICi | fig|1411691.4.peg.2040 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X00786 Genomic DNA Translation: CAA25364.1 U70214 Genomic DNA Translation: AAB08663.1 U00096 Genomic DNA Translation: AAC73347.1 AP009048 Genomic DNA Translation: BAA77912.2 Z12832 Genomic DNA Translation: CAA78292.1 |
PIRi | D64749, RDECER |
RefSeqi | NP_414778.1, NC_000913.3 WP_000893278.1, NZ_SSZK01000050.1 |
3D structure databases
SMRi | P07004 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4259768, 49 interactors 851022, 4 interactors |
DIPi | DIP-10568N |
IntActi | P07004, 17 interactors |
STRINGi | 511145.b0243 |
2D gel databases
SWISS-2DPAGEi | P07004 |
Proteomic databases
jPOSTi | P07004 |
PaxDbi | P07004 |
PRIDEi | P07004 |
Genome annotation databases
EnsemblBacteriai | AAC73347; AAC73347; b0243 BAA77912; BAA77912; BAA77912 |
GeneIDi | 57728932 946680 |
KEGGi | ecj:JW0233 eco:b0243 |
PATRICi | fig|1411691.4.peg.2040 |
Organism-specific databases
EchoBASEi | EB0760 |
Phylogenomic databases
eggNOGi | COG0014, Bacteria |
HOGENOMi | CLU_030231_0_0_6 |
InParanoidi | P07004 |
PhylomeDBi | P07004 |
Enzyme and pathway databases
UniPathwayi | UPA00098;UER00360 |
BioCyci | EcoCyc:GLUTSEMIALDEHYDROG-MONOMER MetaCyc:GLUTSEMIALDEHYDROG-MONOMER |
Miscellaneous databases
PROi | PR:P07004 |
Family and domain databases
CDDi | cd07079, ALDH_F18-19_ProA-GPR, 1 hit |
Gene3Di | 3.40.309.10, 1 hit 3.40.605.10, 1 hit |
HAMAPi | MF_00412, ProA, 1 hit |
InterProi | View protein in InterPro IPR016161, Ald_DH/histidinol_DH IPR016163, Ald_DH_C IPR016162, Ald_DH_N IPR015590, Aldehyde_DH_dom IPR020593, G-glutamylP_reductase_CS IPR012134, Glu-5-SA_DH IPR000965, GPR_dom |
Pfami | View protein in Pfam PF00171, Aldedh, 1 hit |
PIRSFi | PIRSF000151, GPR, 1 hit |
SUPFAMi | SSF53720, SSF53720, 1 hit |
TIGRFAMsi | TIGR00407, proA, 1 hit |
PROSITEi | View protein in PROSITE PS01223, PROA, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PROA_ECOLI | |
Accessioni | P07004Primary (citable) accession number: P07004 Secondary accession number(s): P77428 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1988 |
Last sequence update: | November 1, 1997 | |
Last modified: | February 10, 2021 | |
This is version 184 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families