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UniProtKB - P07001 (PNTA_ECOLI)
Protein
NAD(P) transhydrogenase subunit alpha
Gene
pntA
Organism
Escherichia coli (strain K12)
Status
Functioni
The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane.
1 PublicationCatalytic activityi
- EC:7.1.1.11 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 175 | NAD; via amide nitrogen1 Publication | 1 | |
Binding sitei | 238 | NAD1 Publication | 1 | |
Binding sitei | 257 | NAD; via carbonyl oxygen1 Publication | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 120 – 122 | NAD1 Publication | 3 | |
Nucleotide bindingi | 195 – 197 | NAD1 Publication | 3 |
GO - Molecular functioni
- NAD(P)+ transhydrogenase (AB-specific) activity Source: UniProtKB
- NAD(P)+ transhydrogenase activity Source: GO_Central
- NAD binding Source: EcoCyc
- NADP binding Source: GO_Central
- protein dimerization activity Source: UniProtKB
GO - Biological processi
- NADPH regeneration Source: UniProtKB
- proton export across plasma membrane Source: ComplexPortal
Keywordsi
Molecular function | Translocase |
Ligand | NAD, NADP, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:PNTA-MONOMER |
BRENDAi | 1.6.1.2, 2026 7.1.1.1, 2026 |
Protein family/group databases
TCDBi | 3.D.2.1.1, the proton-translocating transhydrogenase (pth) family |
Names & Taxonomyi
Protein namesi | Recommended name: NAD(P) transhydrogenase subunit alpha (EC:7.1.1.11 Publication)Alternative name(s): Nicotinamide nucleotide transhydrogenase subunit alpha Pyridine nucleotide transhydrogenase subunit alpha |
Gene namesi | Name:pntA Ordered Locus Names:b1603, JW1595 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
Plasma Membrane
- integral component of plasma membrane Source: UniProtKB
- plasma membrane Source: EcoCyc
Other locations
- NAD(P)+ transhydrogenase complex (AB-specific) Source: ComplexPortal
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 401 | CytoplasmicSequence analysisAdd BLAST | 401 | |
Transmembranei | 402 – 422 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 423 – 443 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 444 – 452 | CytoplasmicSequence analysis | 9 | |
Transmembranei | 453 – 473 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 474 – 476 | PeriplasmicSequence analysis | 3 | |
Transmembranei | 477 – 497 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 498 – 510 | CytoplasmicSequence analysisAdd BLAST | 13 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 151 – 161 | Missing : Dimerizes; affinity for PntB unchanged, but maximum transhydrogenation rate lowered significantly. Add BLAST | 11 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000199017 | 1 – 510 | NAD(P) transhydrogenase subunit alphaAdd BLAST | 510 |
Proteomic databases
jPOSTi | P07001 |
PaxDbi | P07001 |
PRIDEi | P07001 |
Interactioni
Subunit structurei
Heterodimer of an alpha (PntA) and a beta (PntB) chain. Alpha subunit serves as the dimerization unit.
2 PublicationsGO - Molecular functioni
- protein dimerization activity Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 4260811, 46 interactors 850974, 2 interactors |
ComplexPortali | CPX-5623, NAD(P) transhydrogenase complex |
DIPi | DIP-366N |
IntActi | P07001, 2 interactors |
STRINGi | 511145.b1603 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P07001 |
SMRi | P07001 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P07001 |
Family & Domainsi
Sequence similaritiesi
Belongs to the AlaDH/PNT family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG3288, Bacteria |
HOGENOMi | CLU_003376_2_1_6 |
InParanoidi | P07001 |
OMAi | YFPMLMT |
PhylomeDBi | P07001 |
Family and domain databases
InterProi | View protein in InterPro IPR008143, Ala_DH/PNT_CS2 IPR008142, AlaDH/PNT_CS1 IPR007886, AlaDH/PNT_N IPR007698, AlaDH/PNT_NAD(H)-bd IPR036291, NAD(P)-bd_dom_sf IPR026255, NADP_transhyd_a IPR024605, NADP_transhyd_a_C |
Pfami | View protein in Pfam PF01262, AlaDh_PNT_C, 1 hit PF05222, AlaDh_PNT_N, 1 hit PF12769, PNTB_4TM, 1 hit |
PIRSFi | PIRSF000203, NADP_transhydrogenase_alpha, 1 hit |
SMARTi | View protein in SMART SM01002, AlaDh_PNT_C, 1 hit SM01003, AlaDh_PNT_N, 1 hit |
SUPFAMi | SSF51735, SSF51735, 1 hit |
TIGRFAMsi | TIGR00561, pntA, 1 hit |
PROSITEi | View protein in PROSITE PS00836, ALADH_PNT_1, 1 hit PS00837, ALADH_PNT_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P07001-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRIGIPRERL TNETRVAATP KTVEQLLKLG FTVAVESGAG QLASFDDKAF
60 70 80 90 100
VQAGAEIVEG NSVWQSEIIL KVNAPLDDEI ALLNPGTTLV SFIWPAQNPE
110 120 130 140 150
LMQKLAERNV TVMAMDSVPR ISRAQSLDAL SSMANIAGYR AIVEAAHEFG
160 170 180 190 200
RFFTGQITAA GKVPPAKVMV IGAGVAGLAA IGAANSLGAI VRAFDTRPEV
210 220 230 240 250
KEQVQSMGAE FLELDFKEEA GSGDGYAKVM SDAFIKAEME LFAAQAKEVD
260 270 280 290 300
IIVTTALIPG KPAPKLITRE MVDSMKAGSV IVDLAAQNGG NCEYTVPGEI
310 320 330 340 350
FTTENGVKVI GYTDLPGRLP TQSSQLYGTN LVNLLKLLCK EKDGNITVDF
360 370 380 390 400
DDVVIRGVTV IRAGEITWPA PPIQVSAQPQ AAQKAAPEVK TEEKCTCSPW
410 420 430 440 450
RKYALMALAI ILFGWMASVA PKEFLGHFTV FALACVVGYY VVWNVSHALH
460 470 480 490 500
TPLMSVTNAI SGIIVVGALL QIGQGGWVSF LSFIAVLIAS INIFGGFTVT
510
QRMLKMFRKN
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X04195 Genomic DNA Translation: CAB37089.1 X66086 Genomic DNA Translation: CAA46884.1 U00096 Genomic DNA Translation: AAC74675.1 AP009048 Genomic DNA Translation: BAA15342.1 |
PIRi | S24380, DEECXA |
RefSeqi | NP_416120.1, NC_000913.3 WP_001300486.1, NZ_SSZK01000001.1 |
Genome annotation databases
EnsemblBacteriai | AAC74675; AAC74675; b1603 BAA15342; BAA15342; BAA15342 |
GeneIDi | 946628 |
KEGGi | ecj:JW1595 eco:b1603 |
PATRICi | fig|1411691.4.peg.659 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X04195 Genomic DNA Translation: CAB37089.1 X66086 Genomic DNA Translation: CAA46884.1 U00096 Genomic DNA Translation: AAC74675.1 AP009048 Genomic DNA Translation: BAA15342.1 |
PIRi | S24380, DEECXA |
RefSeqi | NP_416120.1, NC_000913.3 WP_001300486.1, NZ_SSZK01000001.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1X13 | X-ray | 1.90 | A/B | 2-394 | [»] | |
1X14 | X-ray | 1.94 | A/B | 2-394 | [»] | |
1X15 | X-ray | 2.04 | A/B | 2-394 | [»] | |
2BRU | NMR | - | A/B | 2-394 | [»] | |
AlphaFoldDBi | P07001 | |||||
SMRi | P07001 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260811, 46 interactors 850974, 2 interactors |
ComplexPortali | CPX-5623, NAD(P) transhydrogenase complex |
DIPi | DIP-366N |
IntActi | P07001, 2 interactors |
STRINGi | 511145.b1603 |
Protein family/group databases
TCDBi | 3.D.2.1.1, the proton-translocating transhydrogenase (pth) family |
Proteomic databases
jPOSTi | P07001 |
PaxDbi | P07001 |
PRIDEi | P07001 |
Genome annotation databases
EnsemblBacteriai | AAC74675; AAC74675; b1603 BAA15342; BAA15342; BAA15342 |
GeneIDi | 946628 |
KEGGi | ecj:JW1595 eco:b1603 |
PATRICi | fig|1411691.4.peg.659 |
Organism-specific databases
EchoBASEi | EB0737 |
Phylogenomic databases
eggNOGi | COG3288, Bacteria |
HOGENOMi | CLU_003376_2_1_6 |
InParanoidi | P07001 |
OMAi | YFPMLMT |
PhylomeDBi | P07001 |
Enzyme and pathway databases
BioCyci | EcoCyc:PNTA-MONOMER |
BRENDAi | 1.6.1.2, 2026 7.1.1.1, 2026 |
Miscellaneous databases
EvolutionaryTracei | P07001 |
PROi | PR:P07001 |
Family and domain databases
InterProi | View protein in InterPro IPR008143, Ala_DH/PNT_CS2 IPR008142, AlaDH/PNT_CS1 IPR007886, AlaDH/PNT_N IPR007698, AlaDH/PNT_NAD(H)-bd IPR036291, NAD(P)-bd_dom_sf IPR026255, NADP_transhyd_a IPR024605, NADP_transhyd_a_C |
Pfami | View protein in Pfam PF01262, AlaDh_PNT_C, 1 hit PF05222, AlaDh_PNT_N, 1 hit PF12769, PNTB_4TM, 1 hit |
PIRSFi | PIRSF000203, NADP_transhydrogenase_alpha, 1 hit |
SMARTi | View protein in SMART SM01002, AlaDh_PNT_C, 1 hit SM01003, AlaDh_PNT_N, 1 hit |
SUPFAMi | SSF51735, SSF51735, 1 hit |
TIGRFAMsi | TIGR00561, pntA, 1 hit |
PROSITEi | View protein in PROSITE PS00836, ALADH_PNT_1, 1 hit PS00837, ALADH_PNT_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PNTA_ECOLI | |
Accessioni | P07001Primary (citable) accession number: P07001 Secondary accession number(s): P76888 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1988 |
Last sequence update: | April 1, 1993 | |
Last modified: | May 25, 2022 | |
This is version 193 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families