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Protein

NAD(P) transhydrogenase subunit alpha

Gene

pntA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane.

Catalytic activityi

NADPH + NAD+ = NADP+ + NADH.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei175NAD; via amide nitrogen1 Publication1
Binding sitei238NAD1 Publication1
Binding sitei257NAD; via carbonyl oxygen1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi120 – 122NAD1 Publication3
Nucleotide bindingi195 – 197NAD1 Publication3

GO - Molecular functioni

  • NAD(P)+ transhydrogenase (AB-specific) activity Source: UniProtKB
  • NAD binding Source: EcoCyc
  • NADP binding Source: GO_Central
  • protein dimerization activity Source: UniProtKB

GO - Biological processi

  • NADPH regeneration Source: UniProtKB
  • proton transmembrane transport Source: InterPro

Keywordsi

Molecular functionOxidoreductase
LigandNAD, NADP, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:PNTA-MONOMER
MetaCyc:PNTA-MONOMER
BRENDAi1.6.1.2 2026

Protein family/group databases

TCDBi3.D.2.1.1 the proton-translocating transhydrogenase (pth) family

Names & Taxonomyi

Protein namesi
Recommended name:
NAD(P) transhydrogenase subunit alpha (EC:1.6.1.2)
Alternative name(s):
Nicotinamide nucleotide transhydrogenase subunit alpha
Pyridine nucleotide transhydrogenase subunit alpha
Gene namesi
Name:pntA
Ordered Locus Names:b1603, JW1595
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10744 pntA

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 401CytoplasmicSequence analysisAdd BLAST401
Transmembranei402 – 422HelicalSequence analysisAdd BLAST21
Transmembranei423 – 443HelicalSequence analysisAdd BLAST21
Topological domaini444 – 452CytoplasmicSequence analysis9
Transmembranei453 – 473HelicalSequence analysisAdd BLAST21
Topological domaini474 – 476PeriplasmicSequence analysis3
Transmembranei477 – 497HelicalSequence analysisAdd BLAST21
Topological domaini498 – 510CytoplasmicSequence analysisAdd BLAST13

GO - Cellular componenti

  • integral component of plasma membrane Source: UniProtKB
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi151 – 161Missing : Dimerizes; affinity for PntB unchanged, but maximum transhydrogenation rate lowered significantly. Add BLAST11

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001990171 – 510NAD(P) transhydrogenase subunit alphaAdd BLAST510

Proteomic databases

EPDiP07001
PaxDbiP07001
PRIDEiP07001

Interactioni

Subunit structurei

Heterodimer of an alpha (PntA) and a beta (PntB) chain. Alpha subunit serves as the dimerization unit.2 Publications

GO - Molecular functioni

  • protein dimerization activity Source: UniProtKB

Protein-protein interaction databases

BioGridi4260811, 46 interactors
DIPiDIP-366N
IntActiP07001, 2 interactors
STRINGi316385.ECDH10B_1736

Structurei

Secondary structure

1510
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 5Combined sources4
Helixi20 – 28Combined sources9
Beta strandi32 – 36Combined sources5
Turni37 – 40Combined sources4
Helixi41 – 43Combined sources3
Helixi47 – 53Combined sources7
Beta strandi56 – 58Combined sources3
Helixi60 – 64Combined sources5
Beta strandi65 – 70Combined sources6
Helixi77 – 80Combined sources4
Beta strandi88 – 91Combined sources4
Helixi95 – 97Combined sources3
Helixi99 – 107Combined sources9
Beta strandi111 – 114Combined sources4
Helixi115 – 117Combined sources3
Helixi122 – 127Combined sources6
Helixi129 – 148Combined sources20
Beta strandi156 – 158Combined sources3
Beta strandi161 – 163Combined sources3
Beta strandi167 – 171Combined sources5
Helixi175 – 186Combined sources12
Beta strandi190 – 194Combined sources5
Helixi198 – 200Combined sources3
Helixi201 – 206Combined sources6
Beta strandi209 – 211Combined sources3
Helixi225 – 230Combined sources6
Helixi232 – 248Combined sources17
Beta strandi250 – 254Combined sources5
Beta strandi259 – 261Combined sources3
Helixi269 – 273Combined sources5
Beta strandi280 – 283Combined sources4
Helixi286 – 288Combined sources3
Beta strandi291 – 294Combined sources4
Beta strandi299 – 302Combined sources4
Turni304 – 306Combined sources3
Beta strandi308 – 310Combined sources3
Helixi316 – 318Combined sources3
Helixi320 – 338Combined sources19
Beta strandi341 – 344Combined sources4
Beta strandi350 – 352Combined sources3
Helixi353 – 358Combined sources6
Beta strandi359 – 362Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1X13X-ray1.90A/B2-394[»]
1X14X-ray1.94A/B2-394[»]
1X15X-ray2.04A/B2-394[»]
2BRUNMR-A/B2-394[»]
ProteinModelPortaliP07001
SMRiP07001
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07001

Family & Domainsi

Sequence similaritiesi

Belongs to the AlaDH/PNT family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4108IIE Bacteria
COG3288 LUCA
HOGENOMiHOG000022121
InParanoidiP07001
KOiK00324
OMAiAKVFIMG
PhylomeDBiP07001

Family and domain databases

InterProiView protein in InterPro
IPR008143 Ala_DH/PNT_CS2
IPR008142 AlaDH/PNT_CS1
IPR007886 AlaDH/PNT_N
IPR007698 AlaDH/PNT_NAD(H)-bd
IPR036291 NAD(P)-bd_dom_sf
IPR026255 NADP_transhyd_a
IPR024605 NADP_transhyd_a_C
PfamiView protein in Pfam
PF01262 AlaDh_PNT_C, 1 hit
PF05222 AlaDh_PNT_N, 1 hit
PF12769 PNTB_4TM, 1 hit
PIRSFiPIRSF000203 NADP_transhydrogenase_alpha, 1 hit
SMARTiView protein in SMART
SM01002 AlaDh_PNT_C, 1 hit
SM01003 AlaDh_PNT_N, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR00561 pntA, 1 hit
PROSITEiView protein in PROSITE
PS00836 ALADH_PNT_1, 1 hit
PS00837 ALADH_PNT_2, 1 hit

Sequencei

Sequence statusi: Complete.

P07001-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRIGIPRERL TNETRVAATP KTVEQLLKLG FTVAVESGAG QLASFDDKAF
60 70 80 90 100
VQAGAEIVEG NSVWQSEIIL KVNAPLDDEI ALLNPGTTLV SFIWPAQNPE
110 120 130 140 150
LMQKLAERNV TVMAMDSVPR ISRAQSLDAL SSMANIAGYR AIVEAAHEFG
160 170 180 190 200
RFFTGQITAA GKVPPAKVMV IGAGVAGLAA IGAANSLGAI VRAFDTRPEV
210 220 230 240 250
KEQVQSMGAE FLELDFKEEA GSGDGYAKVM SDAFIKAEME LFAAQAKEVD
260 270 280 290 300
IIVTTALIPG KPAPKLITRE MVDSMKAGSV IVDLAAQNGG NCEYTVPGEI
310 320 330 340 350
FTTENGVKVI GYTDLPGRLP TQSSQLYGTN LVNLLKLLCK EKDGNITVDF
360 370 380 390 400
DDVVIRGVTV IRAGEITWPA PPIQVSAQPQ AAQKAAPEVK TEEKCTCSPW
410 420 430 440 450
RKYALMALAI ILFGWMASVA PKEFLGHFTV FALACVVGYY VVWNVSHALH
460 470 480 490 500
TPLMSVTNAI SGIIVVGALL QIGQGGWVSF LSFIAVLIAS INIFGGFTVT
510
QRMLKMFRKN
Length:510
Mass (Da):54,623
Last modified:April 1, 1993 - v2
Checksum:i801742097BEA6943
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04195 Genomic DNA Translation: CAB37089.1
X66086 Genomic DNA Translation: CAA46884.1
U00096 Genomic DNA Translation: AAC74675.1
AP009048 Genomic DNA Translation: BAA15342.1
PIRiS24380 DEECXA
RefSeqiNP_416120.1, NC_000913.3
WP_001300486.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74675; AAC74675; b1603
BAA15342; BAA15342; BAA15342
GeneIDi946628
KEGGiecj:JW1595
eco:b1603
PATRICifig|1411691.4.peg.659

Similar proteinsi

Entry informationi

Entry nameiPNTA_ECOLI
AccessioniPrimary (citable) accession number: P07001
Secondary accession number(s): P76888
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1993
Last modified: May 23, 2018
This is version 172 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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