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Protein

HTH-type transcriptional regulator MalT

Gene

malT

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Positively regulates the transcription of the maltose regulon whose gene products are responsible for uptake and catabolism of malto-oligosaccharides (PubMed:7040340, PubMed:3305511, PubMed:2538630, PubMed:2524384). Specifically binds to the promoter region of its target genes, recognizing a short DNA motif called the MalT box (5'-GGA[TG]GA-3') (PubMed:2538630, PubMed:2524384). Displays weak ATPase activity, but this activity is not required for promoter binding (PubMed:2524384).4 Publications

Miscellaneous

A study on malT mutants has shown that some of them became nearly completely resistant to Aes repression while still being sensitive to MalY. These mutations are located at positions 38, 220, 243, and 359, most likely defining the interaction patch with Aes on the three-dimensional structure of MalT.1 Publication

Enzyme regulationi

Activated by ATP and maltotriose, which are both required for DNA binding (PubMed:3305511, PubMed:2524384). Negatively regulated by MalY, MalK and Aes, which bind to MalT and block maltotriose activation (PubMed:9822819, PubMed:10692154, PubMed:11867639).5 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi39 – 46ATPUniRule annotation8
DNA bindingi853 – 872H-T-H motifUniRule annotationAdd BLAST20

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • DNA binding Source: UniProtKB-KW
  • identical protein binding Source: IntAct
  • trisaccharide binding Source: EcoCyc

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processCarbohydrate metabolism, Transcription, Transcription regulation
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:PD00237
MetaCyc:PD00237

Names & Taxonomyi

Protein namesi
Recommended name:
HTH-type transcriptional regulator MalTUniRule annotationCurated
Alternative name(s):
ATP-dependent transcriptional activator MalT1 PublicationUniRule annotation
Gene namesi
Name:malT1 PublicationUniRule annotation
Synonyms:malA
Ordered Locus Names:b3418, JW3381
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10562 malT

Pathology & Biotechi

Chemistry databases

DrugBankiDB03793 Benzoic Acid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001841631 – 901HTH-type transcriptional regulator MalTAdd BLAST901

Proteomic databases

PaxDbiP06993
PRIDEiP06993

Expressioni

Inductioni

Expression is induced by the cAMP-activated global transcriptional regulator CRP.1 Publication

Interactioni

Subunit structurei

Monomer in solution (PubMed:3305511, PubMed:10559195). Oligomerizes to an active state in the presence of the positive effectors ATP and maltotriose (PubMed:10559195). Interacts with MalY, MalK and Aes, all of which negatively regulate MalT activity by antagonizing maltotriose binding (PubMed:9822819, PubMed:10692154, PubMed:11867639).5 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi4261189, 24 interactors
852230, 1 interactor
DIPiDIP-10149N
IntActiP06993, 30 interactors
STRINGi316385.ECDH10B_3593

Structurei

Secondary structure

1901
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi442 – 461Combined sources20
Helixi465 – 477Combined sources13
Helixi484 – 501Combined sources18
Helixi504 – 520Combined sources17
Helixi524 – 540Combined sources17
Helixi544 – 560Combined sources17
Helixi568 – 582Combined sources15
Helixi586 – 599Combined sources14
Turni600 – 602Combined sources3
Helixi605 – 608Combined sources4
Helixi609 – 622Combined sources14
Helixi625 – 639Combined sources15
Helixi646 – 662Combined sources17
Helixi666 – 675Combined sources10
Helixi685 – 687Combined sources3
Helixi688 – 700Combined sources13
Helixi704 – 720Combined sources17
Helixi724 – 741Combined sources18
Helixi744 – 761Combined sources18
Helixi765 – 768Combined sources4
Helixi771 – 783Combined sources13
Helixi789 – 802Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HZ4X-ray1.45A437-806[»]
ProteinModelPortaliP06993
SMRiP06993
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP06993

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini829 – 894HTH luxR-typeUniRule annotationAdd BLAST66

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 241DT11 PublicationAdd BLAST241
Regioni242 – 436DT21 PublicationAdd BLAST195
Regioni437 – 806DT32 PublicationsAdd BLAST370
Regioni807 – 901DT41 PublicationAdd BLAST95

Domaini

Consists of four structural domains: the ATP binding site resides in domain I (DT1); DT3 binds the positive effector maltotriose with a low affinity, and the binding affinity is increased in the presence of DT2; the C-terminal domain DT4 contains the helix-turn-helix DNA binding motif. DT1 also contains the region that interacts with MalY (but not with MalK), and the binding site for Aes is also likely to be contained in the N-terminal portion of MalT encompassing DT1 and DT2 (PubMed:12003949). Domain DT3 may mediate oligomerization (PubMed:11709169).2 Publications

Sequence similaritiesi

Belongs to the MalT family.UniRule annotationCurated

Phylogenomic databases

eggNOGiENOG41061ZR Bacteria
COG2909 LUCA
HOGENOMiHOG000218261
InParanoidiP06993
KOiK03556
OMAiSDWVSNA
PhylomeDBiP06993

Family and domain databases

CDDicd06170 LuxR_C_like, 1 hit
Gene3Di1.10.10.10, 1 hit
1.25.40.10, 1 hit
HAMAPiMF_01247 HTH_type_MalT, 1 hit
InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR016032 Sig_transdc_resp-reg_C-effctor
IPR011990 TPR-like_helical_dom_sf
IPR023768 Tscrpt_reg_HTH_MalT
IPR000792 Tscrpt_reg_LuxR_C
IPR036388 WH-like_DNA-bd_sf
PfamiView protein in Pfam
PF00196 GerE, 1 hit
PRINTSiPR00038 HTHLUXR
SMARTiView protein in SMART
SM00421 HTH_LUXR, 1 hit
SUPFAMiSSF46894 SSF46894, 1 hit
SSF48452 SSF48452, 1 hit
SSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00622 HTH_LUXR_1, 1 hit
PS50043 HTH_LUXR_2, 1 hit

Sequencei

Sequence statusi: Complete.

P06993-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLIPSKLSRP VRLDHTVVRE RLLAKLSGAN NFRLALITSP AGYGKTTLIS
60 70 80 90 100
QWAAGKNDIG WYSLDEGDNQ QERFASYLIA AVQQATNGHC AICETMAQKR
110 120 130 140 150
QYASLTSLFA QLFIELAEWH SPLYLVIDDY HLITNPVIHE SMRFFIRHQP
160 170 180 190 200
ENLTLVVLSR NLPQLGIANL RVRDQLLEIG SQQLAFTHQE AKQFFDCRLS
210 220 230 240 250
SPIEAAESSR ICDDVSGWAT ALQLIALSAR QNTHSAHKSA RRLAGINASH
260 270 280 290 300
LSDYLVDEVL DNVDLATRHF LLKSAILRSM NDALITRVTG EENGQMRLEE
310 320 330 340 350
IERQGLFLQR MDDTGEWFCY HPLFGNFLRQ RCQWELAAEL PEIHRAAAES
360 370 380 390 400
WMAQGFPSEA IHHALAAGDA LMLRDILLNH AWSLFNHSEL SLLEESLKAL
410 420 430 440 450
PWDSLLENPQ LVLLQAWLMQ SQHRYGEVNT LLARAEHEIK DIREDTMHAE
460 470 480 490 500
FNALRAQVAI NDGNPDEAER LAKLALEELP PGWFYSRIVA TSVLGEVLHC
510 520 530 540 550
KGELTRSLAL MQQTEQMARQ HDVWHYALWS LIQQSEILFA QGFLQTAWET
560 570 580 590 600
QEKAFQLINE QHLEQLPMHE FLVRIRAQLL WAWARLDEAE ASARSGIEVL
610 620 630 640 650
SSYQPQQQLQ CLAMLIQCSL ARGDLDNARS QLNRLENLLG NGKYHSDWIS
660 670 680 690 700
NANKVRVIYW QMTGDKAAAA NWLRHTAKPE FANNHFLQGQ WRNIARAQIL
710 720 730 740 750
LGEFEPAEIV LEELNENARS LRLMSDLNRN LLLLNQLYWQ AGRKSDAQRV
760 770 780 790 800
LLDALKLANR TGFISHFVIE GEAMAQQLRQ LIQLNTLPEL EQHRAQRILR
810 820 830 840 850
EINQHHRHKF AHFDENFVER LLNHPEVPEL IRTSPLTQRE WQVLGLIYSG
860 870 880 890 900
YSNEQIAGEL EVAATTIKTH IRNLYQKLGV AHRQDAVQHA QQLLKMMGYG

V
Length:901
Mass (Da):103,118
Last modified:July 19, 2003 - v2
Checksum:i9B27CE0F632AD417
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti192 – 193KQ → NE in AAA83888 (PubMed:3015733).Curated2
Sequence conflicti192 – 193KQ → NE in AAA58216 (PubMed:3015733).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13585 Genomic DNA Translation: AAA83888.1
U18997 Genomic DNA Translation: AAA58216.1 Sequence problems.
U00096 Genomic DNA Translation: AAC76443.1
AP009048 Genomic DNA Translation: BAE77873.1
M24342 Genomic DNA Translation: AAA24107.2
V00304 Genomic DNA Translation: CAA23583.1
X02003 Genomic DNA Translation: CAA26034.1
PIRiE65137 RGECMT
RefSeqiNP_417877.1, NC_000913.3
WP_000906961.1, NZ_CP014272.1

Genome annotation databases

EnsemblBacteriaiAAC76443; AAC76443; b3418
BAE77873; BAE77873; BAE77873
GeneIDi947921
KEGGiecj:JW3381
eco:b3418
PATRICifig|1411691.4.peg.3310

Similar proteinsi

Entry informationi

Entry nameiMALT_ECOLI
AccessioniPrimary (citable) accession number: P06993
Secondary accession number(s): Q2M783
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: July 19, 2003
Last modified: May 23, 2018
This is version 166 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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