UniProtKB - P06993 (MALT_ECOLI)
HTH-type transcriptional regulator MalT
malT
Functioni
Positively regulates the transcription of the maltose regulon whose gene products are responsible for uptake and catabolism of malto-oligosaccharides (PubMed:7040340, PubMed:3305511, PubMed:2538630, PubMed:2524384).
Specifically binds to the promoter region of its target genes, recognizing a short DNA motif called the MalT box (5'-GGA[TG]GA-3') (PubMed:2538630, PubMed:2524384).
Displays weak ATPase activity, but this activity is not required for promoter binding (PubMed:2524384).
4 PublicationsMiscellaneous
Activity regulationi
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 39 – 46 | ATPUniRule annotation | 8 | |
DNA bindingi | 853 – 872 | H-T-H motifUniRule annotationAdd BLAST | 20 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- DNA binding Source: UniProtKB-KW
- DNA-binding transcription factor activity Source: UniProtKB-UniRule
- identical protein binding Source: IntAct
- trisaccharide binding Source: EcoCyc
GO - Biological processi
- carbohydrate metabolic process Source: UniProtKB-KW
- positive regulation of carbohydrate metabolic process Source: UniProtKB-UniRule
- positive regulation of transcription, DNA-templated Source: UniProtKB-UniRule
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Carbohydrate metabolism, Transcription, Transcription regulation |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:PD00237 |
Names & Taxonomyi
Protein namesi | Recommended name: HTH-type transcriptional regulator MalTUniRule annotationCuratedAlternative name(s): ATP-dependent transcriptional activator MalT1 PublicationUniRule annotation |
Gene namesi | Name:malT1 PublicationUniRule annotation Synonyms:malA Ordered Locus Names:b3418, JW3381 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000184163 | 1 – 901 | HTH-type transcriptional regulator MalTAdd BLAST | 901 |
Proteomic databases
jPOSTi | P06993 |
PaxDbi | P06993 |
PRIDEi | P06993 |
Expressioni
Inductioni
Interactioni
Subunit structurei
Monomer in solution (PubMed:3305511, PubMed:10559195). Oligomerizes to an active state in the presence of the positive effectors ATP and maltotriose (PubMed:10559195).
Interacts with MalY, MalK and Aes, all of which negatively regulate MalT activity by antagonizing maltotriose binding (PubMed:9822819, PubMed:10692154, PubMed:11867639).
5 PublicationsBinary interactionsi
P06993
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 4261189, 24 interactors 852230, 16 interactors |
DIPi | DIP-10149N |
IntActi | P06993, 30 interactors |
STRINGi | 511145.b3418 |
Structurei
Secondary structure
3D structure databases
SMRi | P06993 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P06993 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 829 – 894 | HTH luxR-typeUniRule annotationAdd BLAST | 66 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 241 | DT11 PublicationAdd BLAST | 241 | |
Regioni | 242 – 436 | DT21 PublicationAdd BLAST | 195 | |
Regioni | 437 – 806 | DT32 PublicationsAdd BLAST | 370 | |
Regioni | 807 – 901 | DT41 PublicationAdd BLAST | 95 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG2909, Bacteria |
HOGENOMi | CLU_006325_3_0_6 |
InParanoidi | P06993 |
PhylomeDBi | P06993 |
Family and domain databases
CDDi | cd06170, LuxR_C_like, 1 hit |
Gene3Di | 1.10.10.10, 1 hit 1.25.40.10, 1 hit 3.40.50.300, 1 hit |
HAMAPi | MF_01247, HTH_type_MalT, 1 hit |
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR016032, Sig_transdc_resp-reg_C-effctor IPR011990, TPR-like_helical_dom_sf IPR041617, TPR_MalT IPR023768, Tscrpt_reg_HTH_MalT IPR000792, Tscrpt_reg_LuxR_C IPR036388, WH-like_DNA-bd_sf |
Pfami | View protein in Pfam PF00196, GerE, 1 hit PF17874, TPR_MalT, 1 hit |
PRINTSi | PR00038, HTHLUXR |
SMARTi | View protein in SMART SM00421, HTH_LUXR, 1 hit |
SUPFAMi | SSF46894, SSF46894, 1 hit SSF48452, SSF48452, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00622, HTH_LUXR_1, 1 hit PS50043, HTH_LUXR_2, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MLIPSKLSRP VRLDHTVVRE RLLAKLSGAN NFRLALITSP AGYGKTTLIS
60 70 80 90 100
QWAAGKNDIG WYSLDEGDNQ QERFASYLIA AVQQATNGHC AICETMAQKR
110 120 130 140 150
QYASLTSLFA QLFIELAEWH SPLYLVIDDY HLITNPVIHE SMRFFIRHQP
160 170 180 190 200
ENLTLVVLSR NLPQLGIANL RVRDQLLEIG SQQLAFTHQE AKQFFDCRLS
210 220 230 240 250
SPIEAAESSR ICDDVSGWAT ALQLIALSAR QNTHSAHKSA RRLAGINASH
260 270 280 290 300
LSDYLVDEVL DNVDLATRHF LLKSAILRSM NDALITRVTG EENGQMRLEE
310 320 330 340 350
IERQGLFLQR MDDTGEWFCY HPLFGNFLRQ RCQWELAAEL PEIHRAAAES
360 370 380 390 400
WMAQGFPSEA IHHALAAGDA LMLRDILLNH AWSLFNHSEL SLLEESLKAL
410 420 430 440 450
PWDSLLENPQ LVLLQAWLMQ SQHRYGEVNT LLARAEHEIK DIREDTMHAE
460 470 480 490 500
FNALRAQVAI NDGNPDEAER LAKLALEELP PGWFYSRIVA TSVLGEVLHC
510 520 530 540 550
KGELTRSLAL MQQTEQMARQ HDVWHYALWS LIQQSEILFA QGFLQTAWET
560 570 580 590 600
QEKAFQLINE QHLEQLPMHE FLVRIRAQLL WAWARLDEAE ASARSGIEVL
610 620 630 640 650
SSYQPQQQLQ CLAMLIQCSL ARGDLDNARS QLNRLENLLG NGKYHSDWIS
660 670 680 690 700
NANKVRVIYW QMTGDKAAAA NWLRHTAKPE FANNHFLQGQ WRNIARAQIL
710 720 730 740 750
LGEFEPAEIV LEELNENARS LRLMSDLNRN LLLLNQLYWQ AGRKSDAQRV
760 770 780 790 800
LLDALKLANR TGFISHFVIE GEAMAQQLRQ LIQLNTLPEL EQHRAQRILR
810 820 830 840 850
EINQHHRHKF AHFDENFVER LLNHPEVPEL IRTSPLTQRE WQVLGLIYSG
860 870 880 890 900
YSNEQIAGEL EVAATTIKTH IRNLYQKLGV AHRQDAVQHA QQLLKMMGYG
V
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 192 – 193 | KQ → NE in AAA83888 (PubMed:3015733).Curated | 2 | |
Sequence conflicti | 192 – 193 | KQ → NE in AAA58216 (PubMed:3015733).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M13585 Genomic DNA Translation: AAA83888.1 U18997 Genomic DNA Translation: AAA58216.1 Sequence problems. U00096 Genomic DNA Translation: AAC76443.1 AP009048 Genomic DNA Translation: BAE77873.1 M24342 Genomic DNA Translation: AAA24107.2 V00304 Genomic DNA Translation: CAA23583.1 X02003 Genomic DNA Translation: CAA26034.1 |
PIRi | E65137, RGECMT |
RefSeqi | NP_417877.1, NC_000913.3 WP_000906961.1, NZ_SSZK01000008.1 |
Genome annotation databases
EnsemblBacteriai | AAC76443; AAC76443; b3418 BAE77873; BAE77873; BAE77873 |
GeneIDi | 947921 |
KEGGi | ecj:JW3381 eco:b3418 |
PATRICi | fig|1411691.4.peg.3310 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M13585 Genomic DNA Translation: AAA83888.1 U18997 Genomic DNA Translation: AAA58216.1 Sequence problems. U00096 Genomic DNA Translation: AAC76443.1 AP009048 Genomic DNA Translation: BAE77873.1 M24342 Genomic DNA Translation: AAA24107.2 V00304 Genomic DNA Translation: CAA23583.1 X02003 Genomic DNA Translation: CAA26034.1 |
PIRi | E65137, RGECMT |
RefSeqi | NP_417877.1, NC_000913.3 WP_000906961.1, NZ_SSZK01000008.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1HZ4 | X-ray | 1.45 | A | 437-806 | [»] | |
SMRi | P06993 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261189, 24 interactors 852230, 16 interactors |
DIPi | DIP-10149N |
IntActi | P06993, 30 interactors |
STRINGi | 511145.b3418 |
Proteomic databases
jPOSTi | P06993 |
PaxDbi | P06993 |
PRIDEi | P06993 |
Genome annotation databases
EnsemblBacteriai | AAC76443; AAC76443; b3418 BAE77873; BAE77873; BAE77873 |
GeneIDi | 947921 |
KEGGi | ecj:JW3381 eco:b3418 |
PATRICi | fig|1411691.4.peg.3310 |
Organism-specific databases
EchoBASEi | EB0557 |
Phylogenomic databases
eggNOGi | COG2909, Bacteria |
HOGENOMi | CLU_006325_3_0_6 |
InParanoidi | P06993 |
PhylomeDBi | P06993 |
Enzyme and pathway databases
BioCyci | EcoCyc:PD00237 |
Miscellaneous databases
EvolutionaryTracei | P06993 |
PROi | PR:P06993 |
Family and domain databases
CDDi | cd06170, LuxR_C_like, 1 hit |
Gene3Di | 1.10.10.10, 1 hit 1.25.40.10, 1 hit 3.40.50.300, 1 hit |
HAMAPi | MF_01247, HTH_type_MalT, 1 hit |
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR016032, Sig_transdc_resp-reg_C-effctor IPR011990, TPR-like_helical_dom_sf IPR041617, TPR_MalT IPR023768, Tscrpt_reg_HTH_MalT IPR000792, Tscrpt_reg_LuxR_C IPR036388, WH-like_DNA-bd_sf |
Pfami | View protein in Pfam PF00196, GerE, 1 hit PF17874, TPR_MalT, 1 hit |
PRINTSi | PR00038, HTHLUXR |
SMARTi | View protein in SMART SM00421, HTH_LUXR, 1 hit |
SUPFAMi | SSF46894, SSF46894, 1 hit SSF48452, SSF48452, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00622, HTH_LUXR_1, 1 hit PS50043, HTH_LUXR_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MALT_ECOLI | |
Accessioni | P06993Primary (citable) accession number: P06993 Secondary accession number(s): Q2M783 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1988 |
Last sequence update: | July 19, 2003 | |
Last modified: | February 23, 2022 | |
This is version 187 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families